Soft Tissue Manipulation Alters RANTES/CCL5 and IL-4 Cytokine Levels in a Rat Model of Chronic Low Back Pain DOI Open Access

Carmela L. Marciano,

Taylor Hiland,

Krista L. Jackson

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(18), P. 14392 - 14392

Published: Sept. 21, 2023

Low back pain (LBP) is a common musculoskeletal complaint that can impede physical function and mobility. Current management often involves medication, but there need for non-pharmacological non-invasive interventions. Soft tissue manipulation (STM), such as massage, has been shown to be effective in human subjects, the molecular mechanisms underlying these findings are not well understood. In this paper, we evaluated potential changes soft levels of more than thirty pro- or anti-inflammatory cytokines following instrument-assisted STM (IASTM) rats with chronic, induced LBP using Complete Freund's Adjuvant. Our results indicate IASTM associated reduced Regulated on Activation, Normal T cell Expressed Secreted (RANTES)/Chemokine (C-C motif) ligand 5 (CCL5) increased Interleukin (IL)-4, which pro-inflammatory factors, respectively, by 120 min post-treatment. was tissue-level C-X-C Motif Chemokine Ligand (CXCL)-5/Lipopolysaccharide-Induced CXC (LIX)-which murine homologue IL-8, CXCL-7, Granulocyte-Macrophage-Colony Simulating Factor (GM-CSF), Intercellular Adhesion Molecule (ICAM)-1, IL1-Receptor Antagonist (IL-1ra), IL-6, Interferon-Inducible Protein (IP)-10/CXCL-10, L-selectin, Tumor Necrosis (TNF)-α, Vascular Endothelial Growth (VEGF) at either 30 Combined, our raise possibility may exert effects improved clinical outcomes potentially beneficial pro-/anti-inflammatory circulation level.

Language: Английский

Role of macrophage in intervertebral disc degeneration DOI Creative Commons

Yiming Dou,

Yiming Zhang, Yang Liu

et al.

Bone Research, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 23, 2025

Language: Английский

Citations

1
Mechanisms of inhibition of nucleus pulposus cells pyroptosis through SDF1/CXCR4-NFkB-NLRP3 axis in the treatment of intervertebral disc degeneration by Duhuo Jisheng Decoction DOI Creative Commons

Daru Guo,

Kang Cheng,

Chao Song

et al.

International Immunopharmacology, Journal Year: 2023, Volume and Issue: 124, P. 110844 - 110844

Published: Aug. 28, 2023

Intervertebral disc degeneration (IVDD) is one of the leading causes lower back pain and most common health problem in world. Inflammasomes, which mainly caused by NLRP3, mediated nucleus pulposus pyroptosis has been discovered to be strongly related IVDD. In addition, Duhuo Jisheng Decoction (DHJSD) anti-inflammatory regulatory effects on NLRP3 inflammasome, but molecular mechanism whether DHJSD can regulate through treat IVDD unclear. this study, we used a bioinformatics way discover regulation IVDD, validated our predictions vitro vivo experiments. Through bioinformatics, found that GSDMD, IL-1βand other hub proteins were highly expressed SD rats, network pharmacology may control cellular senescence, apoptosis, order Additional findings demonstrated could successfully brought imaging histomorphological analysis. Western blot showed key protein pyroptosis, was elevated rat degenerated tissue lipopolysaccharide-treated Nucleus Cells (NPCs), intervention effective reducing LPS-induced inflammatory responses further suppressing expression improve The specific inhibits NPCs via SDF-1/CXCR4-NF-kB-NLRP3 axis. conclusion, revealed intrinsic its value for treatment.

Language: Английский

Citations

19
Molecular mechanism of mechanical pressure induced changes in the microenvironment of intervertebral disc degeneration DOI
Fei Liu, Chao Song, Lei Yang

et al.

Inflammation Research, Journal Year: 2024, Volume and Issue: 73(12), P. 2153 - 2164

Published: Oct. 8, 2024

Language: Английский

Citations

5
Neuroinflammation and Nociception in Intervertebral Disc Degeneration: A Review of Precision Medicine Perspective DOI Creative Commons

Nurul Fariha Zàaba,

Raed H. Ogaili, Fairus Ahmad

et al.

The Spine Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Intervertebral disc (IVD) degeneration is a major cause of low back pain (LBP), which results in disability worldwide. However, the pathogenesis IVD mediating LBP remains unclear. Current conservative treatments and surgical interventions are both to relieve symptoms minimise pain; nevertheless, they unable reverse degeneration. Previous studies have shown that inflammation nociception markers important indicators mechanisms underlying LBP. As such, multi-omics profiling allows discovery these target understand key pathological underpinnings This article provides insights into precision medicine approach for identifying understanding pathophysiology associated with LPB based on severity disease from early mild severe degenerative stages. Molecular IVDs patient stratification at early, mild, stages will contribute identification signalling pathways neuroinflammation, innervation, painful offer an establishing personalised clinical strategies tailored treatment

Language: Английский

Citations

0
Low‐Frequency Cyclic Stretch Upregulates the Expression of Nuclear Factor Erythroid 2‐Related Factor 2 in Human Nucleus Pulposus Cells to Inhibit the Resistin‐Induced Interleukin‐20 Expression DOI Creative Commons

Chia‐Kung Yen,

Hongbo Pan, Hsin‐I Chang

et al.

JOR Spine, Journal Year: 2025, Volume and Issue: 8(1)

Published: Jan. 20, 2025

ABSTRACT Background Resistin may connect obesity and intervertebral disc (IVD) degeneration (IDD) is linked with chronic inflammation. Furthermore, human IDD characterized by high expression of interleukin‐20 (IL‐20). The response nucleus pulposus (NP) cells to tensile forces depends on both the duration magnitude force applied. Nonetheless, interactions among resistin, IL‐20, in regulating function IVD NP remain yet be fully understood. Nuclear factor erythroid 2‐related 2 (NRF2) has been identified as a pleiotropic protein that enhances cellular resistance stress stimuli inflammatory challenges. role NRF2 not well defined, whether influences levels known. Aims This study investigated subjected low‐frequency cyclic stretch stimulation, underlying mechanisms involved. Materials Methods Human were cultured chambers, serum‐starved for 12 h, then 5% strain at 0.1 Hz bioreactor. Control chambers had no stretching. Cells lysed analysis post‐loading. Results stimulation induced IL‐20 dose‐ time‐dependent manner. activation p38 mitogen‐activated kinase, Akt signaling pathways, production toll‐like receptor 4, are necessary render resistin cause release IL‐20. In cells, transcription enzyme‐linked immunosorbent assays revealed led an increase nuclear (NF)‐κB‐DNA binding activities. Exposure inhibited this resistin‐induced NF‐κB expression. Discussion These findings elucidate molecular through which induces also demonstrate can protect against induction.

Language: Английский

Citations

0
ASIC1a mediated nucleus pulposus cells pyroptosis and glycolytic crosstalk as a molecular basis for intervertebral disc degeneration DOI
Chao Song, Fei Liu, Xiaofei Wu

et al.

Inflammation Research, Journal Year: 2025, Volume and Issue: 74(1)

Published: Jan. 28, 2025

Language: Английский

Citations

0
HMGB1-mediated macrophage regulation of NF-κB activation and MMP3 upregulation in nucleus pulposus cells: A critical mechanism in the vicious cycle of intervertebral disc degeneration DOI
Shixin Lu,

Ming Li,

Ziying Cheng

et al.

Cellular Signalling, Journal Year: 2025, Volume and Issue: unknown, P. 111628 - 111628

Published: Jan. 1, 2025

Language: Английский

Citations

0
Pathophysiology and emerging therapeutic strategies for cervical spondylosis: The role of pro-inflammatory mediators, kinase inhibitors, and Organogel based drug delivery systems DOI

Yeshna,

Sachin Kumar Singh,

Monika Monika

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 151, P. 114350 - 114350

Published: Feb. 25, 2025

Language: Английский

Citations

0
Development and Degeneration of the Intervertebral Disc—Insights from Across Species DOI Creative Commons

Kathryn M. Murphy,

Thomas Lufkin, Petra Kraus

et al.

Veterinary Sciences, Journal Year: 2023, Volume and Issue: 10(9), P. 540 - 540

Published: Aug. 24, 2023

Back pain caused by intervertebral disc (IVD) degeneration has a major socio-economic impact in humans, yet historically received minimal attention species other than mice and dogs. However, general growing interest this unique organ prompted the expansion of IVD research rats, rabbits, cats, horses, monkeys, cows, further illuminating complex nature both healthy degenerative states. Application recent biotechnological advancements, including single cell RNA sequencing data analysis methods begun to explain shifting inflammatory signaling, variation cellular subpopulations, differential gene expression, mechanical loading, metabolic stresses which contribute age stress related IVD. This increase across introduces need for chronicling advancements tissue biomarkers within between species. Here we provide comprehensive review alongside existing case reports histo/morphological highlight complexity challenges that is structural importance all vertebrates.

Language: Английский

Citations

9
Effects of Mesenchymal Stem Cell-conditioned Media with Natural Immunomodulatory Agent Resveratrol on Type 1 Diabetes DOI
Krushna Abhale,

Veeranjaneyulu Addepalli,

Shivani Desai

et al.

Current Drug Discovery Technologies, Journal Year: 2024, Volume and Issue: 22(1)

Published: March 12, 2024

Background: Type 1 diabetes mellitus (T1DM) is a condition marked by elevated blood sugar levels and primarily recognized the destruction of beta cells caused an autoimmune attack, which significant characteristic T1DM. Recent studies have demonstrated regenerative potential conditioned medium therapy. In light this, current research sought to assess impact Mesenchymal Stem Cell media (CM) CM with resveratrol (CM+ Resveratrol) on management T1DM in Swiss albino mice. By leveraging modifying existing therapy, this study aims evaluate its effectiveness treating Materials & Methods: Diabetes was induced animals using diabetes-inducing agent streptozotocin (STZ). The were then divided into five groups: Normal control, Disease Control, Resveratrol, Condition Media, + Resveratrol. Treatments given accordingly. period 28 days. During time, monitored for foodwater intake twice week, glucose levels, body weight. At conclusion 28-day period, biochemical estimations performed serum insulin C-peptide anti-inflammatory cytokines pro-inflammatory levels. Additionally, histopathology pancreas performed. Results: test groups showed decrease increase Cpeptide cytokine compared disease group. However, no statistically change within observed terms improvement diabetic symptoms, such as polyphagia, polydipsia, weight loss, treatment group, along pancreatic regeneration, indicated improved secretion. Conclusion: investigation, we concluded that CM+ natural immunomodulators, capacity regenerate injured antidiabetic action, together immunomodulating impact. Nonetheless, future therapy appear be promising.

Language: Английский

Citations

3