Determination of optimal gamma radiation dose for mutation breeding in ’Sabz’ fig (Ficus carica L.) cuttings based on radiosensitivity and phenotypic changes

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0313017 - e0313017

Published: Jan. 15, 2025

The dried fig cv. Sabz of Iran, distinguishes out among the several cultivars for its unique characteristics and excellent properties. aims to this study were 1) Carefully monitoring resulting phenotypic changes in growth patterns, leaf morphology, shoot traits, root characteristics, other relevant traits after irradiated with different gamma rays; 2) Investigating LD 25 , 50 75 GR values at radiation doses chose optimum dose. According our results, was 70 Gy, while approximately 48 95 respectively. Data analysis revealed that higher doses, ranging from 90 led a reduction area hardwood cuttings compared those exposed lower irradiation (10, 20, 30, 40 Gy). In cuttings, plant height gradually decreased line increasing up 60 Gy. Among number particularly influenced by doses. On hand, when Gy dosage, average count dropped 50%. However, subjected dose, surged 90.7% comparison control treatment. Additionally, 63 internode length, 67 56 aerial biomass. number, volume, biomass 46 57 51 An based on indicated height, exhibited greater sensitivity radioactivity portions fig. biological responses ‘Sabz’ fig, is suitable dose initial mutagenesis studies.

Language: Английский

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Differential Participation of Plant Ribosomal Proteins from the Small Ribosomal Subunit in Protein Translation under Stress

Biomolecules, Journal Year: 2023, Volume and Issue: 13(7), P. 1160 - 1160

Published: July 21, 2023

Upon exposure to biotic and abiotic stress, plants have developed strategies adapt the challenges imposed by these unfavorable conditions. The energetically demanding translation process is one of main elements regulated reduce energy consumption selectively synthesize proteins involved in establishment an adequate response. Emerging data shown that ribosomes remodel stresses. In Arabidopsis thaliana, consist approximately eighty-one distinct ribosomal (RPs), each which encoded two seven genes. Recent research has revealed a mutation given single RP can not only affect functions itself but also influence properties ribosome, could bring about changes varying degrees. However, pending question whether some RPs enable preferentially translate specific mRNAs. To reveal role from small subunit (RPS) translation, we novel approach visualize effect RPS silencing on reporter mRNA (GFP) combined 5’UTR different housekeeping defense genes encoding for NbRPSaA, NbRPS5A, NbRPS24A Nicotiana benthamiana decreased NbRACK1A-silenced plant showed compromised translations antioxidant enzymes. all tested were affected NbRPS27D-silenced plants. These findings suggest may be potentially control protein translation.

Language: Английский

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Enemies or Allies? Hormetic and Apparent Non-Dose-Dependent Effects of Natural Bioactive Antioxidants in the Treatment of Inflammation

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1892 - 1892

Published: Feb. 4, 2024

This review aims to analyze the emerging number of studies on biological media that describe unexpected effects different natural bioactive antioxidants. Hormetic effects, with a biphasic response depending dose, or activities are apparently non-dose-dependent, have been described for compounds such as resveratrol, curcumin, ferulic acid linoleic acid, among others. The analysis reported confirms incidence these types which should be taken into account by researchers, discarding initial interpretations imprecise methodologies measurements. enhance research mechanisms action, particularly those studied in field basic research, will help us understand causes unusual behaviors, inactivation signaling pathways immune defense system. Antioxidative and anti-inflammatory addressed ways go beyond mere statistical approach. In this work, some may explain understanding revised, paying special attention ability selected (curcumin, acid) form metal complexes activity media.

Language: Английский

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Molecular Mechanisms of Cold Stress Response in Cotton: Transcriptional Reprogramming and Genetic Strategies for Tolerance

Plant Science, Journal Year: 2025, Volume and Issue: unknown, P. 112390 - 112390

Published: Jan. 1, 2025

Language: Английский

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Arabidopsis thaliana DNA Damage Response Mutants Challenged with Genotoxic Agents—A Different Experimental Approach to Investigate the TDP1α and TDP1β Genes

Genes, Journal Year: 2025, Volume and Issue: 16(1), P. 103 - 103

Published: Jan. 19, 2025

Background/Objectives: DNA damage response (DDR) is a highly conserved and complex signal transduction network required for preserving genome integrity. repair pathways downstream of DDR include the tyrosyl-DNA phosphodiesterase1 (TDP1) enzyme that hydrolyses phosphodiester bond between tyrosine residue topoisomerase I (TopI) 3′-phosphate end DNA. A small TDP1 subfamily, composed TDP1α TDP1β, present in plants. The aim this work was to investigate role two genes context. Methods: series Arabidopsis thaliana single double mutants defective sog1, e2fb, pol2A, atm, atr genes, treated with genotoxic agents camptothecin (CPT, inhibitor TopI) NSC120686 (NSC, TDP1), were used. These compounds specifically used due their known impact on function. effect treatments assessed via phenotypic analyses included germination percentage, speed, seedling growth. Subsequently, expression TDP1β monitored through qRT-PCR. Results: Overall, gathered data indicate atm mutant sensitive NSC120686, both phenotypically concerning gene profiles. Alternatively, upregulation pol2a, supports its implication replication stress response. Conclusions: current study demonstrates induced by CPT NSC has genotype-dependent reflected differential early development.

Language: Английский

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Genome-wide characterization of Chrysanthemum indicum TCP genes reveals a positive role for CiTCP23 in salt response tolerance

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 225, P. 120567 - 120567

Published: Jan. 27, 2025

Language: Английский

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Dynamic changes in the transcriptome of tropical region-originated king grasses in response to cold stress

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 18, 2025

Cold acclimatization in tropical region-originated plants involves complex gene expression reprogramming to adapt fluctuating temperatures. However, the molecular mechanisms and networks regulating cold tolerance king grass remain largely unknown. To address this, we established a full-length reference transcriptome of enhance assembly quality performed multiple time-point transcriptomic analyses following treatment at 4°C. Differentially expressed genes (DEGs) transcription factors (TFs) involved stress response were identified analyzed through clustering co-expression network analysis. A total 13,056 DEGs classified into nine clusters via k-means The exhibited three distinct phases: early (before 3 h), middle (6-24 late (48-72 h). Early-responsive enriched glycolipid metabolism photosynthesis, middle-stage carbohydrate metabolism, late-stage stress, osmotic endogenous stimuli responses. Key regulators ICE-CBF-COR signaling module, including 13 positive negative regulators, identified. further revealed mutual regulatory interactions within this highlighting its role adaptation. Our findings provide insights grass, offering genetic basis for modifying regulators. This research contributes broader understanding low-temperature adaptive supports future breeding strategies improved tolerance.

Language: Английский

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Early Warning Signs in Tree Crowns as a Response to the Impact of Drought

Forests, Journal Year: 2025, Volume and Issue: 16(3), P. 405 - 405

Published: Feb. 24, 2025

The interaction between trees’ water needs during drought and the signals that appear in their canopies is not fully understood. first visually detectable signs, which we describe as early warning tree canopies, are often noticeable at glance. When these signs become widely apparent, decline already underway. In this study, focus on identifying visible of stress crowns, such very small leaves, premature needle/leaf discolouration abscission, defoliation. We provide guidance recognising initial offer specific examples, comprehensively analyse each signal. Our crowns intense prolonged droughts, confirmed by calculating Standardised Precipitation Evapotranspiration Index (SPEI). findings based 20 years (2004–2024) continuous fieldwork data collection from permanent sample plots Serbia, was conducted part International Co-operative Programme Assessment Monitoring Air Pollution Effects Forests (ICP Forests). also a comprehensive review literature key related to address. This research further motivated observed summer 2024 due extreme climatic events, classify year one hottest recorded Serbia. However, still cannot conclusively determine trees will die back solely signals, some manage withstand severe conditions. Nonetheless, widespread appearance indicators clear significant ecosystem instability, potentially leading individual or larger groups.

Language: Английский

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Evaluation of Physiological and Biochemical Responses of Four Tomato (Solanum lycopersicum L.) Cultivars at Different Drought Stress Levels

Agronomy, Journal Year: 2025, Volume and Issue: 15(3), P. 653 - 653

Published: March 5, 2025

Drought, one of the abiotic stress factors that threatens world food security, destructively limits growth and development agricultural plants. Therefore, determining drought-resistant cultivars is vital importance against increasing climate change. Tomato (Solanum lycopersicum L.) most important economic plants grown worldwide. In this study, different drought tolerances (10% PEG (Polyethylene Glycol 6000) water scarcity) were applied to four commercial tomato (Rio Grande, Falcon, H−2274, Tyfrane F1) effects evaluated within scope physiological (germination percentage, shoot length, root fresh weight, dry total chlorophyll content, relative content) biochemical (protein amount, superoxide dismutase (SOD), peroxidase activity (POX), catalase (CAT), hydrogen peroxide content (H2O2) lipid peroxidation (TBARs)) parameters. According research results, it was determined leads decreased root–shoot lengths, weights, antioxidant enzyme activities in Falcon H−2274 cultures, TBARs H2O2 amounts. While which an indicator stress, shows status plant, systems are evidence resilience defense mechanisms cultures. context, cultivar had significantly reduced length (21%, 37%), (20%, 30%), (7%, 23%), weight (51%, 49%) (9%, 29%) under scarcity application; contrast these reductions, (2%, 14%) (3%, 15%) increased compared control, proving a susceptible cultivar. On other hand, slight decrease (1%, 3%), increase (6%), intense CAT (50%, 67%) SOD (30%), but level (5%, 22%) (11%, 15%), detected Rio Grande treatment resistant can be effectively water-scarce areas. It perception stress. As result, when levels terms parameters, tolerance as > F1 ≈ Falcon. responses for selection strategies plant productivity conditions.

Language: Английский

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A Potential Common Pyrroline-5-Carboxylate Synthetase (P5CS) Gene As A Stress Marker For qRT-PCR In Legume Plants

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Journal Year: 2025, Volume and Issue: 8(2), P. 739 - 753

Published: March 12, 2025

Leguminosae (or Fabaceae), commonly known as legumes and including plants of great agricultural ecological importance, are important plant families with a very wide distribution worldwide. Stress is one the main environmental factors affecting growth productivity cultivated plants. Plants designed to minimize impact stress resistance at biochemical level by producing osmolytes such proline protect its components. Pyrroline-5-carboxylate synthetase (P5CS) key enzyme in biosynthesis pathway involved initial step production. In this study, common primer was identified enable qRT-PCR-based studies an alternative methods for determining increase content. With aim, plants, Phaseolus vulgaris, Lens culinaris, Cicer arietinum, Glycine max, Pisum sativum, Medicago sativa, were exposed three different stresses (drought, heat, salt). Proline amounts samples determined biochemically then gene expression levels calculated using primer. our results, significantly increased all samples. Relative analysis results also correlated results. conclusion, study revealed existence marker that can be used determine level, which indicating some legume under stress. Our findings show investigating genes help understand responses molecular level. addition, analysis, especially methods, provides faster more precise This allows observe dynamic changes active shorter time allow us plant's readiness or early

Language: Английский

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