Genome-wide identification and expression analysis of the WRKY transcription factors related to sesquiterpenes biosynthesis in Atractylodes lancea DOI Creative Commons
Liang Hua, Weiwei Liu, Zhiqiang Zhao

et al.

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16

Published: May 15, 2025

Introduction Atractylodes lancea (Thunb.) DC., a widely utilized herb in traditional Chinese medicine, contains sesquiterpenoids and polyacetylenes as its primary bioactive components. The WRKY gene family plays critical role regulating various biological processes plants. However, the molecular mechanism underlying AlWRKY regulation of terpenoid synthesis unclear. Methods members were identified through bioinformatics approaches. Gene structures, motifs, phylogenetic relationships analyzed. Subsequently, their expression profiles across different geographical origins investigated using transcriptome data. Furthermore, preliminary validation was performed via methyl jasmonate treatment docking, with focus on AlWRKY20 AlWRKY37 genes. Results In this study, 65 genes conserved domains A. classified into three groups: Group I (17 members), II (33 III (15 members). Tissue-specific profiling revealed five rhizome-enriched ( AlWRKY13 , AlWRKY21 AlWRKY49 ) highly expressed Hubei accessions compared to Jiangsu accessions, co-expression analysis demonstrated strong correlation 16 AlTPS Quantitative PCR (qPCR) confirmed specific upregulation rhizomes, consistent accumulation patterns sesquiterpenes (hinesol, γ-eudesmol, elemol). Methyl (MeJA) induction experiments (12 h) coordinated AlTPS70 AlTPS71 concomitant significantly increased cis-β-farnesene α-curcumene content. Molecular docking binding affinities AlTPS70/AlTPS71 promoter promoter. Subcellular localization that both are localized nucleus. These results suggest likely function regulators sesquiterpene biosynthesis, positively production modulation. Discussion This study lays groundwork for further exploration mechanisms functional transcription factors .

Language: Английский

Alternative splicing of CsbHLH133 regulates geraniol biosynthesis in tea plants DOI
Long Cheng,

Gefei Tu,

Huicong Ma

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: 120(2), P. 598 - 614

Published: Aug. 29, 2024

Geraniol is one of the most abundant aromatic compounds in fresh tea leaves and contributes to pleasant odor products. Additionally, it functions as an airborne signal that interacts with other members ecosystem. To date, regulation geraniol biosynthesis plants remains be investigated. In this study, a correlation test content its glycosides gene expression data revealed nudix hydrolase, CsNudix26, transcription factor, CsbHLH133 are involved biosynthesis. vitro enzyme assays metabolic analyses genetically modified confirmed CsNudix26 responsible for formation geraniol. Yeast one-hybrid, dual-luciferase reporter, EMSA were used verify binding promoter. Overexpression enhanced accumulation, whereas silencing reduced transcript levels content. Interestingly, CsbHLH133-AS, produced by alternative splicing, was discovered proved primary expressed response various environmental stresses. Furthermore, release found affected factors alter patterns CsbHLH133-AS. Our findings indicate distinct splicing regulate different regulatory factors.

Language: Английский

Citations

5
Comprehensive analysis of NAC transcription factors in Scutellaria baicalensis and their response to exogenous ABA and GA3 DOI

Huan He,

Qiuyue Li,

Liang Fang

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 244, P. 125290 - 125290

Published: June 10, 2023

Language: Английский

Citations

12
CsNAC17 enhances resistance to Colletotrichum gloeosporioides by interacting with CsbHLH62 in Camellia sinensis DOI Creative Commons
Rui Han,

Huiling Mei,

Qiwei Huang

et al.

Horticulture Research, Journal Year: 2024, Volume and Issue: 12(2)

Published: Oct. 14, 2024

The pathogen Colletotrichum gloeosporioides causes anthracnose, a serious threat to tea trees around the world, particularly in warm and humid regions. RNA-Seq data have previously indicated NAC transcription factors are involved anthracnose resistance, but underlying mechanisms remain unclear. BiFC, Split-LUC, Co-IP assays validated interaction between CsbHLH62 CsNAC17 identified through yeast two-hybrid (Y2H) screening. or overexpression enhanced as well levels of H2O2, hypersensitivity, cell death Nicotiana benthamiana. NBS-LRR gene CsRPM1 is regulated by binding directly its promoter (i.e. CACG, CATGTG), while facilitates CsNAC17's increases transcriptional activity CsRPM1. Additionally, transient silencing plant leaves using virus-induced (VIGS) system resulted decreased resistance anthracnose. Conversely, significantly against Based on these results, it appears that CsRPM1, contributing increased resistance.

Language: Английский

Citations

4
Multiomics Analysis Reveals the Genetic Basis of Volatile Terpenoid Formation in Oolong Tea DOI
Kun Chai, Shuai Chen, Pengjie Wang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(49), P. 19888 - 19899

Published: Dec. 4, 2023

Oolong tea has gained great popularity in China due to its pleasant floral and fruity aromas. Although numerous studies have investigated the aroma differences across various cultivars, genetic mechanism is unclear. This study performed multiomics analysis of three varieties suitable for oolong others with different processing suitability. Our revealed that contained higher levels cadinane sesquiterpenoids. PanTFBS was developed identify variants transcription factor binding sites (TFBSs). We found CsDCS gene had two TFBS promoter sequence a single nucleotide polymorphism (SNP) coding sequence. Integrating data on variations, expression, protein-binding indicated might be pivotal involved biosynthesis These findings advance our understanding factors formation offer insights into enhancement aroma.

Language: Английский

Citations

10
Zooming into CTC black-tea wine metabolites: A GC-MS-based study DOI Creative Commons
Soumya Majumder, Arındam Ghosh, Sourav Chakraborty

et al.

Acta Universitatis Sapientiae Alimentaria, Journal Year: 2025, Volume and Issue: 17, P. 15 - 38

Published: Jan. 16, 2025

This research was designed to propose a report on fermentation metabolomics of CTC (crush-tear-curl) tea wine (TW), yeast-fermented broth sugared black infusion. The gas chromatography-mass spectrometry analysis the revealed presence thirty-five metabolites, including major compound glycerine with some potential antioxidant molecules and other bioactive agents (4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-; furfural; furfuryl alcohol; succinic acid; levulinic palmitic tyrosol, pyruvaldehyde; 1-hexadecanol). role metabolites in physicochemical, biochemical, medicinal properties TW has been discussed. Biomolecules responsible for flavour were as follows: derivatives; acetic, levulinic, succinic, acids, etc. – which might develop sweet, caramel-like, astringent, slightly sour wine-like taste. Furthermore, basis yeast metabolism, possible biosynthesis pathways aiming metabolomics. outcome this study cross-verified suggesting its acceptability. As fields both science continue evolve, findings may encourage technology product development from that also boost growth industry.

Language: Английский

Citations

0
Elucidating the regulatory network of valerenic acid biosynthesis in Valeriana officinalis hairy roots under different light qualities: A coexpression perspective DOI Creative Commons
Arash Mokhtari,

Morteza Ebrahimi,

Pejman Azadi

et al.

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 226, P. 120625 - 120625

Published: Feb. 5, 2025

Language: Английский

Citations

0
A global overview of transcriptome dynamics during the late stage of flower bud development in Camellia oleifera DOI Creative Commons
Huiyun Song,

Zhihao Duan,

Heqiang Huo

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 24, 2025

Camellia oleifera Abel., recognized as a significant oil plant, is of immense potential health and economic value. Due to the self-incompatibility C. oleifera, pollination relies on cross-pollination from other flowers. Additionally, asynchronous flowering periods individual plants result in low fruit set yield, which limits broader cultivation utilization this species. The study investigated dynamic changes regulatory patterns different tissues within flower buds bud development flowering, employing multi-faceted approach that included cellular dissection, analysis hormone content, transcriptome analysis. This demonstrates ABA SA, rather than GA3, IAA, ZT, acts potentially effective endogenous agents promote later stages development, critical period for maturation pollen embryo sacs; while ZT plays more role early development. Transcriptome indicated C.oleifera primarily regulates late via regulating genes involved starch sucrose metabolism petals, monoterpene synthesis ABC transporters pistils stamens. WGCNA identified four key modules associated with stamens stage buds, also screened out core genes, including CoBMY7/8, CoTPP6/10, CoG8H7/11, are regulation time. These findings enhance our understanding developmental stamens, pistils, petals during oleifera. Moreover, they provide foundation manipulating time improving by expression future studies.

Language: Английский

Citations

0
Deep transcriptome and metabolome analysis to dissect untapped spatial dynamics of specialized metabolism in Saussurea costus (Falc.) Lipsch DOI
Aasim Majeed, Romit Seth, Balraj Sharma

et al.

Functional & Integrative Genomics, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 28, 2025

Language: Английский

Citations

0
Over-expression of AeWRKY2 promotes oleanolic acid and hederagenin accumulation in Aralia elata DOI
Honghao Xu, Hongfei Liu, Ye Liu

et al.

Biochimie, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Multi-Omics Analysis Revealed the Accumulation of Flavonoids and Shift of Fungal Community Structure Caused by Tea Grafting (Camellia sinensis L.) DOI Creative Commons
Yue Liu, Jun Liu,

Yiping Tian

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(8), P. 1176 - 1176

Published: April 10, 2025

Grafting is an important approach to improving tea plant varieties, and grafting can result in changes secondary metabolites. However, the shifting pattern of metabolites between scions, rootstocks, non-grafted leaves unclear. We employed “Yuncha 1” as scion “Zijuan” rootstock with significant differences leaf color traits reveal influences on generation transcriptional activities. By non-targeted metabolomic analysis, we identified that led obvious shift scion, rootstock, leaves. Importantly, found significantly enhanced accumulation flavonoids, vital component for flavor quality, purple color, health-beneficial effect Zijuan tea. Via transcriptomics, key biosynthetic genes DFR EC 1.1.1.219 synthesizing flavonoids were compared Zijuan. Concurrently, ANS biodegrading was suppressed These results revealed mechanism during grafting. In addition, after possessed no influence bacterial community diversity, but slightly fungal diversity scions. driven by rootstocks a higher diversity. This study systematically reveals which provides novel comprehensive understanding theoretical basis breeding using

Language: Английский

Citations

0