Aquaculture Reports, Journal Year: 2024, Volume and Issue: 40, P. 102598 - 102598
Published: Dec. 27, 2024
Language: Английский
Digestive Diseases and Sciences, Journal Year: 2024, Volume and Issue: 69(10), P. 3729 - 3741
Published: Aug. 7, 2024
The Bacillus-derived cyclic lipopeptides (surfactin, iturin, and fengycin) form potent Heterogeneous Lipopeptide Micelle (HeLM) complexes. HeLM is a small molecule that has been shown to have immunomodulatory effects. However, how regulates inflammation not clear, moreover its application Inflammatory Bowel Disease (IBD), specifically Ulcerative Colitis (UC), tested before. To use murine model of IBD determine the effects related molecular mechanisms action. was induced in mice by administration 4% Dextran Sodium Sulfate. Three preparations were against negative positive controls: Purified HeLM, wild-type strain produces it, an isogenic mutant does produce HeLM. Clinical, biochemical, histological scoring systems used assess severity colitis. RT-qPCR cell cultures levels signaling. Fecal samples processed for metagenomic analysis. strain, significantly decreased colitis as determined disease activity index (DAI), mouse histology (MCHI), fecal calprotectin, colonic length. This effect seen mutant. found be agonist TLR-2, seemingly activating Toll-Like Receptor 2/IL-10 pathway, with subsequent downregulation inflammatory cytokines (TNF-α, IL-1β, IL-6). At higher concentrations inhibited lipopolysaccharide ligands from TLR-4. reduction due microbiome modulation, had previously hypothesized. Our results indicate ameliorates TLR-2-induced IL-10 production possibly via inhibition lipopolysaccharide.
Language: Английский
Citations
3
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11267 - 11267
Published: Oct. 19, 2024
Discovered in late 2019, the SARS-CoV-2 coronavirus has caused largest pandemic of 21st century, claiming more than seven million lives. In most cases, COVID-19 disease by virus is relatively mild and affects only upper respiratory tract; it often manifests itself with fever, chills, cough, sore throat, but also less-common symptoms. patients do not require hospitalization, fully recover. However, some infection leads to development a severe form COVID-19, which characterized life-threatening complications affecting lungs, other organs systems. particular, various forms thrombotic are common among COVID-19. The mechanisms for remain unclear. Accumulated data indicate that pathogenesis based on disruptions functioning innate immune key role primary response viral assigned two These pattern recognition receptors, primarily members toll-like receptor (TLR) family, complement system. Both systems first engage fight against launch whole range aimed at its rapid elimination. Normally, their joint activity destruction pathogen recovery. these can cause an excessive inflammatory dangerous body. turn, inflammation entails activation damage vascular endothelium, as well hypercoagulable state observed seriously ill Activation endothelium hypercoagulation lead thrombosis and, result, tissues. Immune-mediated termed "immunothrombosis". this review, we discuss detail features immunothrombosis associated potential underlying mechanisms.
Language: Английский
Citations
3
Journal of Pharmacology and Experimental Therapeutics, Journal Year: 2024, Volume and Issue: unknown, P. JPET - 002006
Published: June 7, 2024
While acute inflammation serves essential functions in maintaining tissue homeostasis, chronic is causally linked to many diseases. Macrophages are a major cell-type that orchestrates inflammatory processes. During inflammation, macrophages undergo polarization and activation, thereby mobilizing pro-inflammatory anti-inflammatory transcriptional programs regulate ensuing macrophage functions. Fatty acid binding protein 5 (FABP5) lipid chaperone highly expressed macrophages. FABP5 deletion implicated driving towards an phenotype, yet signaling pathways regulated by macrophage-FABP5 have not been systematically profiled. We leveraged proteomic phosphoproteomic approaches characterize modulated M1 M2 polarized bone marrow derived (BMDMs). Stable isotope labeling amino acids (SILAC) based analysis of wild-type (WT) knockout (KO) BMDMs revealed numerous differentially proteins phosphoproteins. impacted downstream associated with cytokine production, oxidative stress, kinase activity. Toll-like receptor 2 (TLR2) emerged as novel target pharmacological inhibition blunted TLR2-mediated activation pathways, ascribing role for TLR2 signaling. This study represents comprehensive characterization the impact upon landscape BMDMs. Loss altered responses, function, work provides foundation future studies seeking investigate therapeutic potential pathophysiological states resulting from dysregulated Significance Statement employed quantitative profiles obtained fatty mice. Our findings multiple compared controls, notably those related inflammation. These results expand our understanding function support recent highlighting targeting treat
Language: Английский
Citations
1
Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11
Published: July 18, 2024
Introduction Coronavirus (CoV) has become a public health crisis that causes numerous illnesses in humans and certain animals. Studies have identified the small, lipid-bound structures called extracellular vesicles (EVs) as mechanism through which viruses can enter host cells, spread, evade host’s immune defenses. EVs are able to package carry viral compounds, including proteins, genetic substances, lipids, receptor proteins. We proposed coronavirus could alter EV production content, well influence biogenesis composition cells. Methods In current research, Crandell-Rees feline kidney (CRFK) cells were infected with (FCoV) an exosome-free media at multiplicity of infection (MOI) 2,500 infectious units (IFU) 48 h 72 time points. Cell viability was analyzed found be significantly decreased by 9% (48 h) 15% (72 due FCoV infection. isolated ultracentrifugation, surface morphology via Scanning Electron Microscope (SEM). Results NanoSight particle tracking analysis (NTA) confirmed mean sizes control 131.9 nm 126.6 nm, while infected-derived 143.4 120.9 h, respectively. Total DNA, RNA, protein levels determined both incubation points; however, total increased h. Expression specific markers such TMPRSS2, ACE2, Alix, TSG101, CDs (29, 47, 63), TLRs (3, 6, 7), TNF-α, others altered infection-derived when compared control-derived after Discussion Our findings suggested affects progression disease evolution. One purpose studying various animal coronaviruses close contact is provide significant information about development, transmission, adaptation. Hence, this study suggests diagnostic therapeutic applications CoVs, understanding prevent future outbreaks.
Language: Английский
Citations
1
Journal of Endodontics, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 1, 2024
Language: Английский
Citations
1
Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 8335 - 8353
Published: Nov. 1, 2024
Vestibular schwannomas (VSs) exhibit a range of tumor behaviors, such as growth patterns and auditory dysfunction. Recent research has offered insights into the inflammatory microenvironment in modulating dynamics. This study investigates role genes immune infiltration VS pathogenesis.
Language: Английский
Citations
1
Experimental Hematology, Journal Year: 2024, Volume and Issue: 136, P. 104272 - 104272
Published: July 6, 2024
Macrophages are fascinating immune cells involved in a variety of processes both health and disease. Although they were first discovered characterized by their functions as professional phagocytes antigen-presenting cells, it is now clear that macrophages have multiple roles within embryonic development, tissue homeostasis, regulation inflammation, host response to pathogens insults. Interestingly, macrophages, or macrophage-like exist organisms, from echinoderms humans, present also species lack an adaptive system hematopoietic stem (HSCs). In mammals, can be generated bone marrow precursors through monocyte intermediate, but known during earlier waves the embryo. Seeding tissues at different times, contribute organogenesis homeostasis. where before HSC specification, seem important component generative microenvironment. There many excellent reviews reporting current knowledge on ontogeny adult tissues. Here, we aim summarize development across most used animal models, with special focus developmental hematopoiesis.
Language: Английский
Citations
1
Anesthesia & Analgesia, Journal Year: 2024, Volume and Issue: 138(6), P. e44 - e45
Published: May 20, 2024
Vandyck, Kofi B. MD; Mazzeffi, Michael A. MD, MPH, MSc; Tanaka, Kenichi MSc Author Information
Language: Английский
Citations
0
Medical Immunology (Russia), Journal Year: 2024, Volume and Issue: 26(4), P. 711 - 716
Published: July 25, 2024
TLR2 is an exceptional pattern-recognizing receptor because of its ability to heterodimerise with different types TLRs, which allows it recognize a wide range molecular structures on the surface pathogens. Polymorphisms in genes involved TLRs signaling cascade may be factor host susceptibility development inflammation, affecting outcome number infectious diseases and immune diseases. The variant Arg753Gln (rs5743708) gene most characterized missense mutation coding region TIR domain, involves substitution arginine for glutamine at position 753 protein sequence. This functionally significant leads altered associated inflammatory responses. In this study, we investigated association polymorphism level expression nonagenarians. study included 82 Frailty was detected 41 subjects using short physical performance battery, registration test ≤ 7 points. It shown that carriage Gln allele statistically significantly increased risk developing frailty; patients Arg/Gln genotype have 12.8-fold higher chance geriatric syndrome. Arg Arg/Arg were found protective factors frailty Analysis nonagenarians revealed 2.79-fold increase relative donors. Evaluation groups presence absence showed 1.4-fold genotype, 1.3 times than group 1.6 Gln/Gln genotype. frequency occurrence due receptor. necessary conduct further functional genetic studies.
Language: Английский
Citations
0
IntechOpen eBooks, Journal Year: 2024, Volume and Issue: unknown
Published: July 31, 2024
Type I interferons are a class of potent and tightly regulated cytokines important for antiviral anti-tumoural innate adaptive immunity. Dysregulated production can have serious neurologic consequences as exemplified in family rare diseases called type interferonopathies. Interferonopathies represent group genetically determined conditions characterised by upregulated interferon causing spectrum neuroinflammatory systemic manifestations. This chapter delves into the historical discovery interferons, their role immunity, subsequent identification interferonopathies placing emphasis on mechanisms dysfunction that often dominate clinical picture. The insights gained from studying these offer valuable lessons neurodegenerative neuropsychiatric which demonstrate considerable overlap with interferonopathies, underscoring broader significance more common diseases. Relevant therapeutic strategies targeting this pathway discussed, emphasising need brain-penetrant approaches.
Language: Английский
Citations
0