hAMSCs regulate EMT in the progression of experimental pulmonary fibrosis through delivering miR-181a-5p targeting TGFBR1

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 5, 2025

Pulmonary fibrosis (PF) is a common and multidimensional devastating interstitial lung disease. The development of novel more effective interventions for PF an urgent clinical need. A previous study has found that miR-181a-5p plays important role in the PF, human amniotic mesenchymal stem cells (hAMSCs) exert potent therapeutic potential on PF. However, whether hAMSCs act by delivering its detailed mechanism still remain unknown. Thus, this was designed to investigate underlying possible bleomycin (BLM)-induced mouse model, co-culture system A549 epithelial transition (EMT) focusing effects collagen deposition, EMT, cell cycle regulation. with different expression levels were constructed. BLM (4 mg/kg) used create while TGF-β1 induce construct EMT model. Furthermore, deposition during assessed vivo vitro. We exerted anti-fibrotic effect BLM-induced Moreover, also protective TGFβ1-induced ameliorated promoting proliferation, reducing apoptosis, attenuating through paracrine effects. regulated targeting TGFBR1. Our findings reveal first time inhibit EMT. Mechanistically, hMASCs achieved

Language: Английский

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The Relationship Between Differential Expression of Non-coding RNAs (TP53TG1, LINC00342, MALAT1, DNM3OS, miR-126-3p, miR-200a-3p, miR-18a-5p) and Protein-Coding Genes (PTEN, FOXO3) and Risk of Idiopathic Pulmonary Fibrosis

Biochemical Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Language: Английский

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Deciphering the interplay: circulating cell-free DNA, signaling pathways, and disease progression in idiopathic pulmonary fibrosis

3 Biotech, Journal Year: 2025, Volume and Issue: 15(4)

Published: March 29, 2025

Language: Английский

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Crocin-I mitigates diquat-induced pulmonary fibrosis via activation of the SIRT3/FOXO3a pathway

Biomedicine & Pharmacotherapy, Journal Year: 2025, Volume and Issue: 186, P. 118043 - 118043

Published: April 8, 2025

Language: Английский

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A Systematic Review of Mortality Risk Prediction Models for Idiopathic Pulmonary Fibrosis

British Journal of Hospital Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 22

Published: April 21, 2025

Aims/Background Idiopathic pulmonary fibrosis (IPF) is associated with an increased mortality risk. However, the factors that contribute to this risk remain unknown. This study aimed systematically review existing predictive models for IPF-related and evaluate prognostic patient outcomes. Methods A comprehensive literature search was conducted on PubMed, Cochrane Library, Web of Science, Embase studies IPF prediction published between 1 January 1984 15 November 2024. Two independent reviewers screened, extracted, cross-checked data. The bias model applicability were also evaluated. Results total 17 identified. area under receiver operating characteristic (ROC) curve (AUC) ranged from 0.728 0.907, while validation results 0.750 0.920. concordance index (C-index) 10 more than 0.7, indicating good performance. encompassed a incorporating 3 8 combined variables, most frequently included predictors being forced vital capacity as percentage predicted value (FVC%pred), carbon monoxide diffusion (DLCO%pred), gender, age, six-minute walk test (6MWT) results, dyspnea severity. Conclusion Current in exploratory phase, generally high bias. Furthermore, lack external some limits their generalizability. Future research should focus improving enhance clinical application.

Language: Английский

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The role of epithelial-mesenchymal transition in pulmonary fibrosis: lessons from idiopathic pulmonary fibrosis and COVID-19

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 13, 2024

Despite the tremendous advancements in knowledge of pathophysiology and clinical aspects SARS-CoV-2 infection, still many issues remain unanswered, especially long-term effects. Mounting evidence suggests that pulmonary fibrosis (PF) is one most severe complications associated with COVID-19. Therefore, understanding molecular mechanisms behind its development helpful to develop successful therapeutic strategies. Epithelial mesenchymal transition (EMT) cell specific variants endothelial (EndMT) mesothelial (MMT) are physio-pathologic cellular reprogramming processes induced by several infectious, inflammatory biomechanical stimuli. Cells undergoing EMT acquire invasive, profibrogenic proinflammatory activities secreting extracellular mediators. Their activity has been implicated pathogenesis PF a variety lung disorders, including idiopathic (IPF) Aim this article provide an updated survey mechanisms, emphasis on EMT-related processes, genesis IFP

Language: Английский

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Spatial transcriptomics unveils the in situ cellular and molecular hallmarks of the lung in fatal COVID-19

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 4, 2024

ABSTRACT Severe Coronavirus disease 2019 (COVID-19) induces heterogeneous and progressive diffuse alveolar damage (DAD) highly disrupting lung tissue architecture homeostasis, hampering management leading to fatal outcomes. Characterizing DAD pathophysiology across progression is of ultimate importance better understand the molecular cellular features driving different patterns optimize treatment strategies. To contextualize interplay between cell types assess their distribution, spatial transcriptomics (ST) techniques have emerged, allowing unprecedented resolution investigate tissues. this end, post-mortem provides valuable insights into composition relationships at time death. Here, we leveraged VisumST technology in COVID-19 induced acute proliferative lungs including control samples with normal morphological appearance, unravel immunopathological mechanisms underlying DAD, providing novel communication events COVID-19. We report a loss endothelial types, pneumocytes type I natural killer cells coupled continuous increase myeloid stromal cells, mostly peribronchial fibroblasts, over progression. Spatial organization analysis identified variable compartments, ranging from major compartments defined by lineages increased more specific compartmentalization immune-specific clusters spectrum. Importantly, spatially informed ligand-receptor interaction (LRI) revealed an intercellular signature defining lungs. Transcription factor (TF) activity enrichment TGF-B pathway as driver, highlighting SMAD3 SMAD7 TFs role during fibrosis. Integration deregulated LRIs allowed us propose downstream intracellular signaling suggesting potential therapeutic targets. Finally, spatio-temporal trajectories provided epithelium regeneration program, characterizing markers II differentiation towards I. In conclusion, provide characterization upon progression, identifying hallmarks that may help patient management.

Language: Английский

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Five New Indole Alkaloid Derivatives from Deep-Sea Fungus Aspergillus fumigatus AF1

Marine Drugs, Journal Year: 2024, Volume and Issue: 23(1), P. 4 - 4

Published: Dec. 25, 2024

One new gliotoxin derivative fumianthrogliotoxin (1), one indoquizoline alkaloid N3-(methyl propionate) (2), and three novel indole alkaloids, anthroxyindole (3), (±)-asperfumiindole A (4), B (5), together with 16 known compounds (6–21), were isolated from the culture of deep-sea derived fungus Aspergillus fumigatus AF1. Their chemical structures absolute configurations determined through analysis NMR data in combination electronic circular dichroism (ECD) calculations other spectroscopic analyses. Compounds 2–11 13–21 evaluated for anti-pulmonary fibrosis activity. 8 13 displayed significant downregulation mRNA expression levels all molecular markers (COL1A1, α-SMA FN1), compound exhibiting best performance among tested compounds.

Language: Английский

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