Proteomics analysis of porcine endometrial cell-derived extracellular vesicles involved in embryo attachment
Molecular & Cellular Proteomics,
Journal Year:
2025,
Volume and Issue:
unknown, P. 100942 - 100942
Published: March 1, 2025
Maternal-embryo
interactions
play
a
critical
role
in
early
mammalian
development,
with
extracellular
vesicles
(EVs)
playing
key
intercellular
communication.
Recent
studies
have
focused
on
the
mechanisms
by
which
maternal-derived
factors,
such
as
RNA,
proteins,
and
metabolites
influence
gap
junctions,
EVs,
direct
cell-to-cell
interactions,
contributing
to
embryonic
development.
In
this
study,
using
proteomics
approach,
we
investigated
impact
of
EVs
secreted
from
porcine
endometrial
cells
(pEECs)
their
protein
cargoes
We
characterized
isolated
pEECs
(pEEC-EVs)
during
diestrus
stage
nanoparticle
tracking
analysis
cryo-transmission
electron
microscopy.
Furthermore,
effects
pEEC-EVs
or
without
hormone
treatment
vitro
attachment
hatched
blastocysts
were
evaluated.
The
rate
embryos
was
significantly
higher
for
group
than
control
(23.0
±
1.7%
vs.
36.9
1.9%
pEEC-EVs,
respectively).
altered
expression
proteins
involved
cellular
organization,
transport,
immunity.
Proteomic
revealed
distinct
biological
processes
between
groups:
supported
cytoskeletal
organization
adhesion,
while
hormone-treated
enriched
immune
regulation,
stress
response
pathways.
Key
signaling
pathways,
including
VEGFA-VEGFR2,
focal
TGF-β,
modulated,
influencing
implantation
embryogenesis.
crucial
maternal-embryo
optimizing
conditions
supporting
embryo-derived
stem
cell
establishment.
These
findings
enhance
our
understanding
EV-mediated
communication
suggest
potential
applications
improving
reproductive
health
assisted
technologies.
Language: Английский
Opportunities and limitations of introducing artificial intelligence technologies into reproductive medicine
V. A. Lebina,
No information about this author
O. Kh. Shikhalakhova,
No information about this author
A. A. Kokhan
No information about this author
et al.
Obstetrics Gynecology and Reproduction,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 14, 2025
Given
the
increasing
problem
of
infertility
in
Russian
Federation,
assisted
reproductive
technologies
(ART)
have
proven
to
be
one
most
effective
treatments
for
this
condition.
Notably,
introduction
ART
methods,
particularly
vitro
fertilization
(IVF),
has
led
markedly
increased
birth
rates
over
past
two
decades.
Studies
show
that
machine
learning
algorithms
can
process
images
embryos
assess
their
quality,
thus
facilitating
selection
viable
among
them
transfer.
There
are
ethical
and
technical
barriers
hindering
widespread
adoption
artificial
intelligence
(AI)
clinical
practice,
including
concerns
data
privacy
as
well
a
need
train
specialists
deal
with
new
technologies.
AI
analyze
vast
amounts
data,
medical
histories
research
results,
more
accurately
predict
pregnancy
outcomes.
This
enables
doctors
make
justified
decisions.
In
future,
will
able
patient
efficiently,
helping
identify
causes
at
earlier
stages.
Language: Английский
Elastography in Reproductive Medicine, a Game-Changer for Diagnosing Polycystic Ovary Syndrome, Predicting Intrauterine Insemination Success, and Enhancing In Vitro Fertilization Outcomes: A Systematic Review
Biomedicines,
Journal Year:
2025,
Volume and Issue:
13(4), P. 784 - 784
Published: March 24, 2025
Background:
Elastography
is
an
ultrasound-based
imaging
technology
that
allows
for
quantitative
measurement
of
tissue
stiffness
and
elasticity.
In
reproductive
medicine,
it
a
potential
non-invasive
method
assessing
ovarian
activity,
uterine
contractility,
endometrial
receptivity.
While
conventional
ultrasound
provides
anatomical
vascular
information,
does
not
assess
biomechanical
properties,
which
are
important
understanding
polycystic
ovary
syndrome
(PCOS),
predicting
intrauterine
insemination
(IUI)
success,
determining
receptivity
in
vitro
fertilization
(IVF).
Methods:
A
systematic
review
was
conducted
accordance
with
the
PRISMA
principles,
protocol
recorded
PROSPERO.
comprehensive
literature
search
across
several
databases
to
uncover
studies
used
real-time
elastography
(RTE)
or
shear
wave
(SWE)
PCOS
diagnosis,
IUI
result
prediction,
evaluation
IVF.
The
risk
bias
assessed
using
ROBINS-I
technique.
Results:
Four
fulfilled
inclusion
criteria.
One
study
indicated
patients
had
considerably
increased
stiffness,
supports
as
diagnostic
marker.
Another
found
flexibility
decreased
contractility
were
related
better
outcomes.
retrospective
cohort
research
discovered
non-uniform
echogenicity
no
influence
on
IVF
results.
Furthermore,
SWE
successfully
evaluated
unexplained
infertility,
higher
being
reduced
implantation
potential.
Conclusions:
gives
real-time,
insights
into
biomechanics,
applications
infertility
diagnosis
ART
improvement.
However,
absence
defined
procedures
confirmed
clinical
criteria
prevent
its
broad
use.
More
large-scale
prospective
investigations
required
improve
elastographic
parameters
define
cutoffs
Language: Английский
A review of artificial intelligence applications in in vitro fertilization
Journal of Assisted Reproduction and Genetics,
Journal Year:
2024,
Volume and Issue:
42(1), P. 3 - 14
Published: Oct. 14, 2024
Language: Английский
Motile Cilia in Female and Male Reproductive Tracts and Fertility
Cells,
Journal Year:
2024,
Volume and Issue:
13(23), P. 1974 - 1974
Published: Nov. 28, 2024
Motile
cilia
are
evolutionarily
conserved
organelles.
In
humans,
multiciliated
cells
(MCCs),
assembling
several
hundred
motile
on
their
apical
surface,
components
of
the
monolayer
epithelia
lining
lower
and
upper
airways,
brain
ventricles,
parts
reproductive
tracts,
fallopian
tube
uterus
in
females,
efferent
ductules
males.
The
coordinated
beating
generates
a
force
that
enables
shift
tubular
fluid,
particles,
or
along
surface
ciliated
epithelia.
Uncoordinated
altered
motion
immotility
may
result
subfertility
even
infertility.
Here,
we
summarize
current
knowledge
regarding
localization
function
MCCs
human
discuss
how
beating-generated
fluid
flow
directly
indirectly
contribute
to
processes
these
organs,
lack
improper
functioning
influence
fertility.
Language: Английский
Add-ons in medically assisted reproduction: from evidence to clinical practice
Human Reproduction,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 27, 2024
Graphical
AbstractThe
live
edition
of
the
ESHRE
Journal
Club
discussion
at
40th
Annual
Meeting
focused
on
good
practice
recommendations
add-ons
in
reproductive
medicine
that
formulated
42
covering
diagnosis
and
diagnostic
tests,
laboratory
tests
interventions,
clinical
management
(ESHRE
Add-Ons
Working
Group
et
al.,
2023)
with
discussion's
central
focal
points
being
recommended
use
management,
quality
evidence
add-on
recommendation
use,
suitable
markers
for
assessing
efficacy,
standardization
treatment
patient
perspectives.
MAR,
medically
assisted
reproduction;
RCTs,
randomized
controlled
trials.Open
new
tabDownload
slide
Language: Английский