ACS Applied Bio Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 12, 2024
Antibiotic
misuse
and
bacterial
resistance
are
pressing
issues
threatening
public
health.
Natural
plant
extracts
with
bactericidal
properties
offer
potential
alternatives
to
reduce
or
replace
antibiotic
use.
This
study
aims
develop
a
thermosensitive
hydrogel
containing
daphnetin
(DAP-TG)
using
poloxamers
407
(P407),
polyvinylpyrrolidone
(PVP),
188
(P188).
We
systematically
evaluated
the
gel's
antibacterial
activity
against
Escherichia
coli
(E.
coli)
Staphylococcus
aureus
(S.
aureus),
as
well
its
mechanisms.
By
examining
gelation
temperature
time,
degradation
in
vitro
release
performance
of
DAP-TG,
we
produced
sustained-release
DAP-TG
rapid
phase
transition
at
(31.6
±
0.1)
°C.
Its
structure
was
characterized
through
Fourier
transform
infrared
(FTIR)
spectroscopy
scanning
electron
microscopy
(SEM).
The
results
indicated
that
DAP
formed
presented
3D
network
spatial
structure.
biocompatibility
explored
hemolysis
test
cytotoxicity
test.
possessed
excellent
biocompatibility.
efficacy
E.
S.
assessed
minimum
inhibitory
concentration
(MIC),
(MBC),
growth
curve,
inhibition
zone
tests.
Results
showed
exhibited
both
aureus,
MIC
values
1.28
0.32
mg/mL.
mechanism
preliminarily
investigation
cell
content
leakage,
AKP
membrane
permeability,
SEM,
ROS
production,
biofilm
activity.
induced
irreversible
damage
membranes
resulting
enhanced
elevated
levels,
inhibited
formation.
Our
indicates
exhibits
effective
vitro,
making
it
promising
candidate
for
applications
food
pharmaceutical
products.
Microorganisms,
Journal Year:
2025,
Volume and Issue:
13(2), P. 401 - 401
Published: Feb. 12, 2025
The
increasing
demand
for
food
safety
and
the
need
to
combat
emerging
foodborne
pathogens
have
driven
development
of
innovative
packaging
solutions.
Active
packaging,
particularly
those
incorporating
antimicrobial
agents,
has
emerged
as
a
promising
approach
enhance
preservation
safety.
Among
these
bacteriophages
(phages)
gained
significant
attention
due
their
specificity,
efficacy,
natural
origin.
This
manuscript
explores
role
active
in
protecting
against
pathogens,
with
particular
focus
on
bacteriophages.
review
overviews
recent
advances
antimicrobials
followed
by
detailed
discussion
bacteriophages,
including
classification,
mode
action,
multidisciplinary
applications,
use
agents
packaging.
also
highlights
commercially
available
bacteriophage-based
products
addresses
challenges
limitations
associated
integration
into
materials.
Despite
potential,
issues
such
stability,
regulatory
hurdles,
consumer
acceptance
remain
critical
considerations.
In
conclusion,
represent
tool
enhancing
safety,
but
further
research
innovation
are
needed
overcome
existing
barriers
fully
realize
potential
industry.
Frontiers in Molecular Biosciences,
Journal Year:
2024,
Volume and Issue:
11
Published: July 9, 2024
Background
Diabetic
foot
ulcers
are
the
most
common
and
serious
complication
of
diabetes
mellitus,
high
morbidity,
mortality,
disability
which
greatly
diminish
quality
life
patients
impose
a
heavy
socioeconomic
burden.
Thus,
it
is
urgent
to
identify
potential
biomarkers
targeted
drugs
for
diabetic
ulcers.
Methods
In
this
study,
we
downloaded
datasets
related
from
gene
expression
omnibus.
Dysregulation
mitophagy-related
genes
was
identified
by
differential
analysis
weighted
co-expression
network
analysis.
Multiple
machine
algorithms
were
utilized
hub
genes,
novel
artificial
neural
model
assisting
in
diagnosis
constructed
based
on
their
transcriptome
patterns.
Finally,
that
can
target
using
Enrichr
platform
molecular
docking
methods.
Results
702
differentially
expressed
ulcers,
enrichment
showed
these
associated
with
mitochondria
energy
metabolism.
Subsequently,
hexokinase-2,
small
ribosomal
subunit
protein
us3,
l-lactate
dehydrogenase
A
chain
as
multiple
learning
validated
diagnostic
performance
validation
cohort
independent
present
study
(The
areas
under
roc
curve
0.671,
0.870,
0.739,
respectively).
Next,
training
good,
0.924
0.840,
respectively.
retinoic
acid
estradiol
promising
anti-diabetic
targeting
hexokinase-2
(−6.6
−7.2
kcal/mol),
us3
(−7.5
−8.3
(−7.6
−8.5
kcal/mol).
Conclusion
The
chain,
emphasized
critical
roles
treatment
through
dimensions,
providing
Frontiers in Microbiology,
Journal Year:
2025,
Volume and Issue:
16
Published: Jan. 27, 2025
Currently,
multidrug-resistant
(MDR)
bacterial
wound
infections
(WIs)
are
an
extremely
challenging
clinical
problem
for
physicians.
Recently,
compared
to
traditional
single
liquid
delivery
drugs,
the
study
of
five
novel
drug
systems
(i.e.,
hydrogel,
liposomes,
electrospun
fibers,
nanoparticles
and
nanoemulsion)
phages
their
encoded
lysins
in
WI
management
has
become
a
hot
topic.
To
assess
current
landscape
these
emerging
technologies,
we
conducted
comprehensive
literature
search
across
PubMed,
Scopus
Web
Science
up
July
2024,
using
terms
such
as
“phage,”
“lysin,”
“wound,”
“hydrogel,”
“liposomes,”
“fibers,”
“nanoparticles,”
“nanoemulsion.”
The
criteria
included
original
studies
management.
findings
highlighted
positive
effects
management,
significantly
reducing
populations,
accelerating
healing
at
injury
site.
However,
available
on
remains
limited,
particularly
lysins.
In
conclusion,
application
showed
great
potential
combating
MDR
WIs.
Infectious Medicine,
Journal Year:
2025,
Volume and Issue:
4(1), P. 100168 - 100168
Published: Feb. 6, 2025
Phages,
including
the
viruses
that
lyse
bacterial
pathogens,
offer
unique
therapeutic
advantages,
their
capacity
to
antibiotic-resistant
bacteria
and
disrupt
biofilms
without
harming
host
microbiota.
The
lack
of
new
effective
antibiotics
growing
limitations
existing
have
refocused
attention
on
phage
therapy
as
an
option
in
complex
clinical
cases
such
burn
wounds,
cystic
fibrosis,
pneumonia.
This
review
describes
preclinical
studies
which
has
been
both
human
veterinary
medicine,
agricultural
context.
In
addition,
critical
challenges,
narrow
range
bacteriophages,
possibility
resistance,
regulatory
constraints
widespread
use
therapy,
are
addressed.
Future
directions
include
optimizing
through
strategies
ranging
from
cocktails
broadening
genetic
modification,
using
phages
vaccines
or
biocontrol
agents.
future,
if
can
be
efficiently
delivered,
maintained
a
stable
state,
phage-antibiotic
synergy
achieved,
will
much
needed
treatment
options.
However,
successful
implementation
within
current
standards
practice
also
require
considerable
development
infrastructure
greater
public
acceptance.
closing,
this
highlights
promise
backup
substitute
for
antibiotics.
It
proposes
role
significant
adjunct
to,
even
replacement
for,
treating
multidrug-resistant
infections.
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(7), P. 2914 - 2914
Published: March 23, 2025
The
ideal
bacteriophages
(phages)
for
the
treatment
of
bacterial
disease
(phage
therapy)
would
bypass
evolution
to
phage
resistance.
However,
this
feature
(called
a
hyper-aggression
feature)
has
never
been
observed
our
knowledge.
Here,
we
microbiologically
characterize,
fractionate,
genomically
classify,
and
perform
electron
microscopy
newly
isolated
Bacillus
thuringiensis
0524phi7-1,
which
find
have
feature.
Even
visible
colonies
are
cleared.
Phage
0524phi7-1
also
three
other
features
classified
under
(four-feature-hyper-aggressive
phage).
(1)
forms
plaques
that,
although
sometimes
beginning
as
semi-turbid,
eventually
clear.
(2)
Clear
continue
enlarge
days.
No
phage-resistant
bacteria
detected
in
cleared
zones.
(3)
Plaques
smaller
satellite
plaques,
even
gels
so
concentrated
that
implied
satellite-generating
motion
is
not
host
generated.
In
addition,
reveals
myophage
with
an
isometric,
91
nm-head
(diameter)
210
nm-long
contractile
tail,
undergoes
extensive
aggregation,
inhibits
typical
studies
physiology.
genome
linear
double-stranded
DNA,
which,
by
sequencing,
157.103
Kb
long:
family,
Herelleviridae;
genus,
tsarbombavirus.
data
suggest
hypothesis
both
swimming
hibernation.
Techniques
isolating
better
phages
therapy.
