Clinical and laboratory investigations of a sudden death syndrome in newborn Boer goats DOI Creative Commons
Maged El-Ashker, Reham Karam,

Shady Shalaby

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 15, 2023

Abstract Background The aim of the present study was to determine cause a sudden death syndrome in Boer goats on an organised farm Dakahlia Governorate, Egypt, and investigate associated haematobiochemical changes. In December 2021, outbreak detected with two hundred adult thirty kids. complaint that seventeen newborn kids died suddenly after showing brief episode apathy, while adults remained healthy showed no systemic disease. As FMD infections occurred simultaneously large ruminant farms region, buccal nasal swabs were taken from thirteen diagnose virus one step RT-PCR. Further typing tests carried out circulating FMDV. Blood samples also A post-mortem examination eight carcasses, internal organs intestinal contents for bacteriological examination. Tissue fixed 10% neutral buffered formalin histopathological Results results confirmed FMDV type O strain, C. perfringens cultured by DNA sequencing as strain B1. alpha toxin gene ( CPA ) detected. affected picture intravascular haemolysis, which is indicative toxin. Conclusions highlights role Clostridia infection complication This first report has been association this valuable goat species. These improve our understanding clinical presentation complicated cases under natural conditions.

Language: Английский

Cu-doped Fe3O4 nanoparticles for efficient detoxification of epsilon toxin: Toward substituting magnetically recyclable detoxifying agent for formaldehyde DOI Creative Commons
Parvaneh Esmaeilnejad-Ahranjani, Marzieh Lotfi, Azadeh Zahmatkesh

et al.

Toxicon, Journal Year: 2024, Volume and Issue: 242, P. 107707 - 107707

Published: April 4, 2024

This research presents the synthesis and characterization of Cu-doped Fe

Language: Английский

Citations

4
Integrated Assessment of Mucilage Impact on Human Health Using the One Health Approach: Prevalence and Antimicrobial Resistance Profiles of Escherichia coli and Clostridium perfringens in the Marmara Sea, Türkiye DOI Creative Commons
Artun Yıbar, Hilal Ay, Fuat Aydın

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(3), P. e42103 - e42103

Published: Jan. 18, 2025

This study employed a One Health approach to assess the potential impact of mucilage on human health by characterizing prevalence and antimicrobial resistance (AMR) profiles Escherichia coli Clostridium perfringens strains isolated during 2021 event in Marmara Sea, Türkiye. Mucilage, gelatinous organic substance exacerbated climate change, disrupts marine ecosystems depleting oxygen, threatening biodiversity, serving as reservoir for pathogenic microorganisms. Surface benthic samples collected from Sea were analysed AMR using genome analysis, BD Phoenix™ 100 automated system, E-test methods. The identified 13 E. one C. strain, harboring 244 six genes 21 eight drug classes, respectively, along with multiple virulence factors (VFs). exhibited four distinct serotypes (O138:H28 [Mu-3], O18:H49 [Mu-4], O128:H12 [Mu-35] O101:H10 [Mu-125]), reported first time Türkiye mucilage. Notably, anaerobic microorganisms like thrived mucilage, underscoring their ecological significance. Seasonal climatic influencing formation amplify its role transmitting antimicrobial-resistant pathogens, posing significant risks public environmental health. findings highlight urgent need continuous monitoring mitigation strategies mucilage-related hazards.

Language: Английский

Citations

0
A Narrative Review on The Beneficial Effects of <i>Lactobacillus</i> Probiotics Against Necrotic Enteritis in Poultry DOI Creative Commons

Sobia Razzaq,

Qazal Hina, Muhammad Muneeb

et al.

Published: March 19, 2025

Necrotic enteritis is an important disease of poultry that causes economic loss to the broiler industry. Clostridium perfringens bacterium responsible for causing necrotic enteritis. Antibiotics are mainly used control C. but due resistance antibiotics banned in many countries like Canada, Hong Kong, and European Union. Many alternatives such as probiotics, essential oils, postbiotics have been developed perfringens. Among them, probiotics very because they can increase beneficial bacteria intestine, create a competitive environment gut region, prevent adhesion colonization pathogenic Probiotics cause immune system modulation, reducing inflammatory markers cytokines. Lactobacillus based also weight gain, improve feed conversion ratio, decrease mortality which turn profit margin. Several studies reported when populations were challenged with then these prevented intestinal lesions, provided anti-inflammatory effects damage villi, did not allow form its colony intestine. The main aim this review paper explain updated information on enteritis, damages caused gut, mechanism actions through Lactobacilli work against

Language: Английский

Citations

0
Broad host range phages target global Clostridium perfringens bacterial strains and clear infection in five-strain model systems DOI Creative Commons
Anisha M. Thanki, Emmanuel Kuffour Osei, Natasha Whenham

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(5)

Published: March 21, 2024

ABSTRACT Clostridium perfringens is a prevalent bacterial pathogen in poultry, and due to the spread of antimicrobial resistance, alternative treatments are needed prevent treat infection. Bacteriophages (phages), viruses that kill bacteria, offer viable option can be used therapeutically C. infections. The aim this study was isolate phages against strains currently circulating on farms across world establish their virulence development potential using host range screening, assays, larva infection studies. We isolated 32 which 19 lysed 80%–92% our global poultry strain collection ( n = 97). these individual different phage combinations quantified liquid culture at multiple doses. then developed multi-strain model, mimic an effective model by industry. tested efficacy 16/32 cocktails model. From this, we identified cocktail consisting CPLM2, CPLM15, CPLS41 most reducing colonization infected larvae when administered before challenge. These data suggest do have significant poultry. IMPORTANCE causes foodborne illness worldwide, 95% human infections linked consumption contaminated meat, including chicken products. In necrotic enteritis, associated mortality rates up 50%. However, treating difficult as bacterium becoming antibiotic-resistant. Furthermore, industry striving toward reduced antibiotic usage. (phages) promising alternative, progress approach, robust suitable laboratory models required. study, targeting found many lyse from chickens worldwide. Consistent with other published studies, systems assayed here, some were combined cocktails, cleared effectively compared use.

Language: Английский

Citations

3
Lumbar Spondylodiscitis Caused by Clostridium perfringens: A Case Report Highlighting Diagnostic Challenges and Conservative Management DOI Open Access
Bruno Rodrigues Cancela,

Dara Mbanze,

João Miranda

et al.

Cureus, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

This case report describes a 70-year-old male with history of hepatic cirrhosis and diabetes who was admitted severe low back pain, fever, sepsis. Initially, the pain appeared consistent muscle strain. However, persistence symptoms, ongoing patient's underlying conditions raised suspicion for more serious pathology. Blood cultures identified Clostridium perfringens, MRI confirmed L4-L5 spondylodiscitis accompanied by an epidural abscess. rare musculoskeletal infection, known its high mortality risk, successfully treated targeted antibiotic therapy alone, as there were no neurological deficits to warrant surgical intervention. After nine weeks treatment, follow-up showed resolution abscess, patient made full clinical recovery. The likely mechanism in this is bacterial translocation driven portal hypertension immune dysregulation associated liver cirrhosis. underscores importance investigating causes patients presenting red flag it demonstrates that conservative management can be effective without involvement, even when caused high-risk pathogens such C. perfringens.

Language: Английский

Citations

0
Novel toxin-based mRNA vaccine against Clostridium perfringens using in silico approaches DOI
Parisa Asadollahi, Behrooz Sadeghi Kalani

Toxicon, Journal Year: 2024, Volume and Issue: 238, P. 107584 - 107584

Published: Jan. 5, 2024

Language: Английский

Citations

3
Clostridial Myonecrosis: A Comprehensive Review of Toxin Pathophysiology and Management Strategies DOI Creative Commons

Hussain Hussain,

Aya Fadel,

Efrain Garcia

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(7), P. 1464 - 1464

Published: July 18, 2024

Clostridial myonecrosis, commonly known as gas gangrene (GG), is a rapidly progressing and potentially fatal bacterial infection that primarily affects muscle soft tissue. In the United States, incidence of GG roughly 1000 cases per year, while, in developing countries, higher. This condition most often caused by

Language: Английский

Citations

3
Utilization of AI – reshaping the future of food safety, agriculture and food security – a critical review DOI
Jerina Rugji, Zeki Erol, Fulya Taşçı

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 45

Published: Dec. 7, 2024

Artificial intelligence is an emerging technology which harbors a suite of mechanisms that have the potential to be leveraged for reaping value across multiple domains. Lately, there increased interest in embracing applications associated with Intelligence positively contribute food safety. These such as machine learning, computer vision, predictive analytics algorithms, sensor networks, robotic inspection systems, and supply chain optimization tools been established several domains safety early warning outbreaks, risk prediction, detection identification pathogens. Simultaneously, ambition toward establishing sustainable system has motivated adoption cutting-edge technologies strengthen security. Given myriad challenges confronting stakeholders their endeavors safeguard security, emerges promising tool capable crafting holistic management strategies This entails maximizing crop yields, mitigating losses, trimming operational expenses. AI models present notable benefits efficiency, precision, uniformity, automation, pattern identification, accessibility, scalability security endeavors. The escalation global trend adopting alternative protein sources edible insects microalgae source reflects growing recognition need resilient systems address population growth, environmental degradation, insecurity. offers range capabilities enhance production consumption proteins like insects, contributing secure system.

Language: Английский

Citations

3
Arginine alleviates Clostridium perfringens α toxin-induced intestinal injury in vivo and in vitro via the SLC38A9/mTORC1 pathway DOI Creative Commons
Xiaohui Wang,

Tong Zhang,

Wenli Li

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: April 4, 2024

Introduction Clostridium perfringens α toxin is a main virulence factor responsible for gut damage in animals. Arginine functional amino acid exhibiting significant immunoregulatory activities. However, the effects and mechanisms of arginine supplementation on toxin-induced intestinal injury remain unclear. Methods In vivo , 256 male Arbor Acres chickens were randomly assigned to 2×2 factorial arrangement, involving diet treatments (with or without 0.3% supplementation) immunological stress challenge). vitro IEC-6 cells treated with presence absence toxin. Moreover, transfected siRNA targeting mTOR SLC38A9 explore underlying mechanisms. Results discussion The results showed that significantly alleviated growth performance impairment, decreases serum immunoglobulin (Ig)A IgG levels, morphology damage. also reduced increase jejunal proinflammatory cytokines interleukin (IL)-1β IL-6 IL-17 mRNA expression. decreased mechanistic target rapamycin (mTOR) solute carrier family 38 member 9 ( SLC38A9) expression, while increased pretreatment mitigated decrease cell viability cytotoxicity apoptosis. upregulation expression inflammation-related C-X-C motif chemokine ligand CXCL ) 10 CXCL11 transforming factor-β TGF-β ), as well apoptosis-related genes B-cell lymphoma-2 associated X protein Bax Bcl-2 lymphoma-extra large Bcl-XL cysteinyl aspartate specific proteinase 3 Caspase-3 ratio . eukaryotic translation initiation 4E (eIF4E)-binding 1 4EBP1 ribosomal S6 kinase S6K Knockdown largely abrogated positive intracellular changes. Furthermore, silencing abolished caused by pretreatment. conclusion, administration attenuated which could be downregulation inflammation via regulating SLC38A9/mTORC1 pathway.

Language: Английский

Citations

2
Formal Single Atom Editing of the Glycosylated Natural Product Fidaxomicin Improves Acid Stability and Retains Antibiotic Activity DOI Creative Commons
Isabella Ferrara, Gleb A. Chesnokov,

Silvia Dittmann

et al.

JACS Au, Journal Year: 2024, Volume and Issue: 4(6), P. 2267 - 2280

Published: May 21, 2024

Fidaxomicin (Fdx) constitutes a glycosylated natural product with excellent antibacterial activity against various Gram-positive bacteria but is approved only for Clostridioides difficile infections. Poor water solubility and acid lability preclude its use other Herein, we describe our strategy to overcome the by introducing acid-stable S-linked glycosides. We direct, diastereoselective modification of unprotected Fdx without need avoid air or moisture. Using newly established approach, was converted single atom exchanged analogue S-Fdx, in which labile O-glycosidic bond noviose sugar replaced stable S-glycosidic bond. Studies structurally diverse set thioglycoside derivatives revealed high potency acyl S-Fdx (MIC range: 0.12–4 μg/mL) Clostridium perfringens 0.06–0.5 μg/mL).

Language: Английский

Citations

2