A case study of the diet-microbiota-parasite interplay in bumble bees DOI
Antoine Gekière, Maryse Vanderplanck, Amanda Hettiarachchi

et al.

Journal of Applied Microbiology, Journal Year: 2023, Volume and Issue: 134(12)

Published: Dec. 1, 2023

Diets and parasites influence the gut bacterial symbionts of bumble bees, but potential interactive effects remain overlooked. The main objective this study was to assess isolated sunflower pollen, its phenolamides, widespread trypanosomatid Crithidia sp. on Bombus terrestris males.Bumble bee males emerged in microcolonies fed either (i) willow pollen (control), (ii) or (iii) spiked with phenolamide extracts from pollen. These were infected by pathogen-free. Using 16S rRNA amplicon sequencing (V3-V4 region), we observed a significant alteration beta diversity not alpha microbial communities compared control Similarly, infection parasite altered males, irrespective diet. By contrast, did observe any phenolamide-enriched Changes indicate dissimilarities taxa between treatment groups, while lack difference demonstrates no changes within each group.Bumble bees harbour consistent microbiota worldwide, our results suggest that are somewhat shaped their diets as well interaction these two factors. This confirms suitable biological surrogates effect diet infections communities.

Language: Английский

Microbiome assembly and maintenance across the lifespan of bumble bee workers DOI
Tobin J. Hammer, August Easton‐Calabria, Nancy A. Moran

et al.

Molecular Ecology, Journal Year: 2022, Volume and Issue: 32(3), P. 724 - 740

Published: Nov. 5, 2022

Abstract How a host's microbiome changes over its lifespan can influence development and ageing. As these temporal patterns have only been described in detail for handful of hosts, an important next step is to compare succession more broadly investigate why it varies. Here we characterize the dynamics stability bumble bee worker gut microbiome. Bumble bees simple host‐specific microbiomes, their microbial may health pollination services. We used 16S rRNA gene sequencing, quantitative PCR metagenomics microbiomes Bombus impatiens workers. also sequenced transcriptomes examine host factors that control At community level, assembly highly predictable similar primary observed human gut. However, at strain partitioning bacterial variants among colonies suggests stochastic colonization events those flies nematodes. find strong differences symbiont species, suggesting ecological members persistence. Finally, show both transcriptome—including expression key immunity genes—stabilize, as opposed senesce, with age. suggest social groups such bees, maintenance contribute inclusive fitness, thus remain under selection even old Our findings provide foundation exploring mechanisms functional outcomes succession.

Language: Английский

Citations

31
Bumble bee microbiota shows temporal succession and increase of lactic acid bacteria when exposed to outdoor environments DOI Creative Commons
Arne Weinhold,

Elisabeth Grüner,

Alexander Keller

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: March 4, 2024

The large earth bumble bee (

Language: Английский

Citations

6
The wild solitary bees Andrena vaga, Anthophora plumipes, Colletes cunicularius, and Osmia cornuta microbiota are host specific and dominated by endosymbionts and environmental microorganisms DOI
Amanda Hettiarachchi, Margo Cnockaert, Marie Joossens

et al.

Microbial Ecology, Journal Year: 2023, Volume and Issue: 86(4), P. 3013 - 3026

Published: Oct. 5, 2023

Language: Английский

Citations

12
Bumble bee gut microbial community structure differs between species and commercial suppliers, but metabolic potential remains largely consistent DOI Creative Commons
Michelle Hotchkiss, Alexandre J. Poulain, Jessica R. K. Forrest

et al.

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

ABSTRACT Bumble bees are key pollinators for natural and agricultural plant communities. Their health performance supported by a core gut microbiota composed of few bacterial taxa. However, the taxonomic composition community structure bumble bee microbiotas can vary with species, environment, origin (i.e., whether colonies come from wild or commercial rearing facility), it is unclear metabolic capabilities therefore as well. Here we used metagenomic sequencing to examine composition, structure, potential across two different Bombus impatiens suppliers, B. , three other species sampled sites within native range all four species. We found that varied between populations origins suppliers. Notably, Apibacter consistently present in some species—suggesting may be previously unrecognized phylotype bees—and lack phylotypes populations. despite variation high-level was largely consistent hosts, including related host performance, though activity remains investigated. IMPORTANCE Our study first compare genome-level whole suppliers In addition, profiled full time. Overall, our results provide new insight into function will help researchers evaluate how well studies conducted one population translate Research on increasing relevance continue discover ways social influence health, decline abundance.

Language: Английский

Citations

0
Captive-rearing changes the gut microbiota of the bumblebee Bombus lantschouensis native to China DOI Creative Commons
Feng Zhou,

Shuning Sun,

Xinxin Song

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18964 - e18964

Published: Feb. 13, 2025

Bumblebees play crucial roles as pollinators in both natural agricultural and ecological systems. Their health overall fitness are significantly affected by the gut microbiota, which can maintain intestinal homeostasis hosts regulating their nutritional metabolism. However, information about diversity of microbiota related functional changes during artificial rearing native species is currently limited. This study investigated dynamic remodeling Chinese bumblebee Bombus lantschouensis under captive rearing, supported 16S rRNA amplicon sequencing bacterial DNA. The typical microbial community composition was detected wild B. , with genus Gilliamella Snodgrassella identified dominant strains. Conversely, captive-reared group showed increased decreased abundance certain microorganisms. populations Bifidobacterium Saccharibacter Lactobacillus including Firm-4 Firm-5, were dramatically after captive-rearing became bacteria, while strikingly reduced. Notably, this found that pathogenic bacteria appeared intestines wild-caught disappeared when host reared conditions. shows bumblebees facilitates physiological metabolism commercial insects.

Language: Английский

Citations

0
Bee Microbiomes: Unveiling Diversity and Social Dynamics in Managed and Wild Bees DOI
Maria Ludovica Saccà, Ilaria Resci,

Giovanni Cilia

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Patterns of Insect Distribution in Fruit Trees of South Romania and Their Role as Bacterial Vectors DOI Creative Commons

Dana S. Copoiu,

Paris Lavín, Corina Iţcuş

et al.

Diversity, Journal Year: 2025, Volume and Issue: 17(4), P. 295 - 295

Published: April 20, 2025

This study is the first investigation of tree–insect–bacteria interactions in southern Romania, documenting distribution 19 insect species across various fruit trees and their insect-associated bacterial diversity. Insect were identified through DNA barcoding, while communities Anthomyia, Botanophila, Drosophila, Scaptomyza insects analyzed via 16S rRNA gene sequencing. diversity varied apple, cherry, plum, peach, quince trees, with most showing tree-specific distribution, except for Drosophila melanogaster, which was found on all tree species. Its presence primarily influenced by development stages rather than temperature changes. comprised 51 genera four phyla, predominantly Pseudomonadota Bacillota, that species, suggesting potential role these flies as vectors. Several pathogenic biomarkers within microbiomes, involvement disease transmission, particularly affecting apple cherry trees. also provides report seven being microbiome characterization dipteran associated regional

Language: Английский

Citations

0
Bee-Associated Beneficial Microbes—Importance for Bees and for Humans DOI Creative Commons
Svetoslav Dimitrov Todorov,

Marcos Vinício Alves,

Gisana Cristina Alves Bueno

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(6), P. 430 - 430

Published: June 6, 2024

Bees are one of the best-known and, at same time, perhaps most enigmatic insects on our planet, known for their organization and social structure, being essential pollination agricultural crops several other plants, playing an role in food production balance ecosystems, associated with high-value-added inputs, a unique universe relation to bees’ microbiota. In this review, we summarize information regarding different varieties bees, emphasis specificity related microbial variations. Noteworthy fructophilic bacteria, lesser-known bacterial group, which use fructose fermentation as main source energy, some strains closely health status. The beneficial properties bacteria may be extendable humans animals probiotics. addition, biotechnological potential ease development new-generation antimicrobials applications biopreservation. concept “One Health” brings together fundamental applied research aim clarifying that connections between components ecosystems must considered part mega-structure, bees iconic example healthy functionality microbiota is directly indirectly production, bee health, quality products, functional prosperity animals. fact, good clearly stable relates humans’ wellbeing, Health”.

Language: Английский

Citations

3
A comparative genomic analysis of Fructobacillus evanidus sp. nov. from bumble bees DOI
Juliana Botero, Charlotte Peeters,

Evelien De Canck

et al.

Systematic and Applied Microbiology, Journal Year: 2024, Volume and Issue: 47(2-3), P. 126505 - 126505

Published: March 22, 2024

Language: Английский

Citations

1
Studies on the novel effects of electron beam treated pollen on colony reproductive output in commercially-reared bumblebees (Bombus terrestris) for mass pollination applications DOI Creative Commons
John Eakins, Mark Lynch, James C. Carolan

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 899, P. 165614 - 165614

Published: July 19, 2023

Commercially-reared bumblebees provide an important pollinator service that helps support food production and security. The deployment of appropriate non-thermal disinfection technology for the bulk treatment pollen collected from honeybees feeding commercial is in order to mitigate against complex diseases unwanted pathogen spillover native bees. High level was achieved using electron (e)-beam dose 100 kGy corresponded 78 % loss cellular viability bee pathogens before as measured by novel vitro use flow cytometry (FCM). Novel findings showed e-beam treated-pollen fed produced fewer females, gynes exhibited absence males when compared control bumblebee colonies were untreated pollen. A similar trend emerged colony reproductive outputs membrane filtered washed Proteomic analysis individual with revealed a differential abundance proteins associated stress, immunity metabolism group. Microbiome gut content differences microbiota between treated studies. This study evaluated impact industrial on disease mitigation where physically shown substantially affect outputs.

Language: Английский

Citations

2