Insect Biochemistry and Molecular Biology, Journal Year: 2024, Volume and Issue: 168, P. 104107 - 104107
Published: March 14, 2024
Language: Английский
Citations
11
PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0318154 - e0318154
Published: Jan. 29, 2025
Insecticide resistance is a major problem in food production, environmental sustainability, and human health. The cotton bollworm Helicoverpa armigera globally distributed crop pest affecting over 300 species. H. has rapidly evolved insecticide resistance, making it one of the most damaging pests worldwide. Understanding genetic basis provides insights to develop tools, such as molecular markers, that can be used slow or prevent evolution resistance. We explore architecture widely insecticide, flubendiamide, using two complementary approaches: genome-wide association studies (GWAS) wild-caught samples quantitative trait locus (QTL) mapping controlled cross susceptible resistant laboratory strains. Both approaches identified on chromosome 2, revealing SNPs within 976 bp monitor field flubendiamide. This was only region linkage mapping, though GWAS revealed additional sites associated with Other loci by populations contained known detoxification genes from ATP-binding cassette family, ABCA1, ABCA3, ABCF2 MDR1. Our findings an oligogenic architecture, contrasting previous reports monogenic ryanodine receptor . work elucidates evolving will contribute developing effective management strategies.
Language: Английский
Citations
1
BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)
Published: Feb. 7, 2025
Abstract Background Molecular genetic approaches have become vital to understanding the evolutionary processes that act on insect pest populations. From mapping development of resistance monitoring and predicting movement, genomic tools can inform enhance management programs. Here, we used whole genome sequencing population genomics unravel novel patterns structure, linkage, selection across a notorious agricultural pest, fall armyworm. Results Our data strongly support existence two genetically distinct strains armyworm in North America, which previously been referred as C-strain R-strain. Although these diverged genetically, find differentiation is not uniform genome. The Z-chromosome appears drive divergence between with high levels linkage observed this chromosome. We also show region containing circadian clock gene implicated allochronic reproductive isolation under strain-specific selection. indicates differ their geographic distributions exhibit sub-structuring indicative unique dispersal patterns. provide first evidence for nuclear major overwintering populations US. Finally, our reveal population-specific regions putative insecticide alleles, could relate biogeography. Conclusions results dyad US, genetically-distinct differing patterns, signatures likely involved resistance. These differences should be considered when devising implementing strategies.
Language: Английский
Citations
1
Biochemical and Biophysical Research Communications, Journal Year: 2023, Volume and Issue: 670, P. 19 - 26
Published: May 26, 2023
Language: Английский
Citations
21
Insects, Journal Year: 2024, Volume and Issue: 15(9), P. 705 - 705
Published: Sept. 16, 2024
Spodoptera frugiperda (J.E. Smith) (Noctuidae: Lepidoptera) is a notable insect pest that invades major cereal crops, causing significant damage and loss. Resistances of 2nd instar larvae two Egyptian field populations S. frugiperda, collected from the Fayoum Giza governments, were measured against eight insecticides, including traditional insecticides (profenofos cypermethrin), bio-insecticides (emamectin benzoate, spinosad, Bacillus thuringiensis), growth regulators (IGRs) (lufenuron, diflubenzuron, methoxyfenozide). In addition, synergistic effects three synergists (Piperonyl butoxide (PBO), diethyl maleate (DEM), triphenyl phosphate (TPP) assessed, activities detoxification enzymes (acetylcholine esterase (AChE), cytochrome P-450 (CYP-450), carboxylesterase (CarE), glutathione-s-transferase (GST) also determined. Resistance surveillance revealed population showed moderate resistance to cypermethrin (RR = 5.75-fold), followed by spinosad 2.62-fold), lufenuron (2.01-fold). On other hand, exhibited only 3.65-fold). Our results emamectin benzoate was most effective insecticide, with an LC50 value 0.003 mg/L for 0.001 population, compared susceptible strain (0.005 mg/L). Among biological thuringiensis least toxic insecticide all tested strains. Synergism assays indicated DEM TPP had effect on (SR 8.00-fold both), PBO 5.71-fold) alone. The assay GST activity significantly (p < 0.05) increased in strains strain. However, no changes observed among CYP-450, CarE, or AChE. findings this study provide substantial insights into tracking managing development Egypt.
Language: Английский
Citations
6
Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(30), P. 16651 - 16660
Published: July 22, 2024
is a significant global pest, and chlorantraniliprole (CAP) extensively used in China for its control. Understanding CAP resistance
Language: Английский
Citations
4
Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 208, P. 106292 - 106292
Published: Jan. 9, 2025
Language: Английский
Citations
0
Pest Management Science, Journal Year: 2025, Volume and Issue: unknown
Published: April 17, 2025
Abstract BACKGROUND Cyantraniliprole, a diamide insecticide, is widely used in Brazil to control sucking and defoliating pests, including the fall armyworm ( Spodoptera frugiperda ), major agricultural pest. However, increasing resistance diamides has raised concerns about long‐term effectiveness of cyantraniliprole. This study aimed (i) assess evolution cyantraniliprole field populations S. , (ii) investigate cross‐resistance other diamides, (iii) analyze role I4790K mutation mechanisms. RESULTS A significant decrease susceptibility was observed Brazil, particularly regions with intensive systems, such as Brazilian Cerrado, from 2017 2023. cyantraniliprole‐resistant strain selected field‐collected population Bahia (BA) using F 2 screening method. The inheritance this autosomal recessive monogenic, 3414‐fold ratio. High flubendiamide, chlorantraniliprole, cyclaniliprole also detected. Molecular analysis confirmed presence homozygous ryanodine receptor gene one mechanisms strain. CONCLUSION These findings highlight decreased accelerating insecticides due cross‐resistance. results underscore urgent need for integrated pest management (IPM) strategies, insecticide rotation monitoring, preserve efficacy insecticides. © 2025 Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf Society Chemical Industry.
Language: Английский
Citations
0
Pest Management Science, Journal Year: 2025, Volume and Issue: unknown
Published: April 25, 2025
Abstract BACKGROUND The invasive fall armyworm ( Spodoptera frugiperda , FAW), a highly destructive pest affecting more than 350 plant species, has recently invaded Europe raising urgent management concerns. Insecticide resistance profiling is essential to support evidence‐based control strategies. In this study, we analyzed target‐site insecticide mutations in FAW populations from Greece inform addition, DNA barcoding through cytochrome oxidase subunit 1 COI ) gene sequencing was used trace the pest's geographic origin and potential invasion pathways. RESULTS All specimens were identified as rice strain, exhibiting two almost balanced haplotypes (Haplotype 1: 58.6%; Haplotype 2: 41.4%), suggesting likely single, genetically diverse source population. Resistance‐associated ABCC2 (A > G single‐nucleotide polymorphism (SNP); up 80.9%) Ace‐1 (F290V: 37.5%; A201S: 3.85%), conferring Bacillus thuringiensis (Bt) organophosphates/carbamates, respectively. By contrast, no resistance‐associated detected for other key insecticides (diamides, pyrethroids, oxadiazines, spinosyns, avermectins), their current efficacy Greece. CONCLUSION This study provides critical baseline monitoring Europe, supporting development of sustainable integrated strategies line with European Union Green Deal. Continuous molecular diagnostics, alongside complementary bioassays, recommended mitigate impact on agriculture. © 2025 Author(s). Pest Management Science published by John Wiley & Sons Ltd behalf Society Chemical Industry.
Language: Английский
Citations
0
Neotropical Entomology, Journal Year: 2024, Volume and Issue: 53(4), P. 937 - 954
Published: May 1, 2024
Language: Английский
Citations
3