Insect Gut Microbiota and Pesticide Degradation in Response to Innate Metabolites- a Review DOI Open Access
Saleem Jaffar, Muhammad Yasin,

Muhammad Mazahir

et al.

Indian Journal of Entomology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 12

Published: Jan. 23, 2024

Insects host a diverse microbiota in their gut, encompassing bacteria, fungi, viruses, and archaea, influencing physiology, nutrition, overall health. The composition of these microbial communities varies with factors like insect species, diet, the environment. Insect gut serve pivotal roles such as aiding digestion, synthesizing essential nutrients, safeguarding against pathogens, detoxifying toxins, including insecticides. A particularly promising facet function lies metabolism These microbiotas can either augment or diminish insecticide toxicity through mechanisms enzymatic breakdown, sequestration, target site alteration, modulation insect's immune response. Understanding interactions is paramount for devising sustainable pest management strategies. This review explores into microbiota, impact on susceptibility, potential use metabolites eco-friendly control. We explore pesticide degradation mechanisms, consequences disruption role microbiota-produced shaping efficacy. Ultimately, we highlight manipulation strategy to enhance effectiveness combat resistance management.

Language: Английский

A comparative genomic analysis at the chromosomal-level reveals evolutionary patterns of aphid chromosomes DOI Creative Commons
Chen Huang,

Bingru Ji,

Zhaohui Shi

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: March 13, 2025

Genomic rearrangements are primary drivers of evolution, promoting biodiversity. Aphids, an agricultural pest with high species diversity, exhibit rapid chromosomal evolution and diverse karyotypes. These variations have been attributed to their unique holocentric chromosomes parthenogenesis, though this hypothesis has faced scrutiny. In study, we generated a chromosomal-level reference genome assembly the celery aphid (Semiaphis heraclei) conducted comparative genomic analysis, revealing varying rates among lineages, positively correlating diversity. Aphid X undergone frequent intra-chromosomal recombination, while autosomes show accelerated inter-chromosomal recombination. Moreover, considering both inter- rearrangements, increased autosomal rearrangement may be common across Aphidomorpha. We identified that expansion DNA transposable elements short interspersed nuclear (SINEs), coupled gene loss duplication associated karyotypic instability (such as RIF1, BRD8, DMC1, TERT), play crucial roles in evolution. Additionally, our analysis revealed mutation detoxification families S. heraclei key factor adapting host plant chemical defenses. Our results provide new insights into evolutionary patterns aphids, aiding understanding diversity adaptive Chromosomal-level comparisons

Language: Английский

Citations

0
Acaricide Resistance Monitoring and Structural Insights for Precision Tetranychus urticae Management DOI Creative Commons

Said Kewedar,

Qing Chen, Timothy W. Moural

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(5), P. 440 - 440

Published: April 23, 2025

The two-spotted spider mite (Tetranychus urticae) is a highly destructive and economically significant pest in agricultural, horticultural, ornamental agroecosystems worldwide, including hop (Humulus lupulus) mint (Mentha spp.) fields the Pacific Northwest (PNW) region of United States. Repeated acaricide applications rotations have led to widespread resistance, resulting control failures. In this study, we investigated mechanisms resistance four different acaricides (bifenthrin, bifenazate, etoxazole, abamectin) across 23 field-collected TSSM populations by integrating diagnostic bioassays, genetic screening for resistance-associated mutations, structural modeling, molecular docking. Several kdr mutations mutation combinations were detected TuVGSC all tested populations. G132A Tucytb was identified 68.75% 40% populations, while I1017F TuCHS 1 found 94% 100% Structural analysis revealed key interactions between target proteins both wild-type mutant variants, providing novel insights into functional impacts these mutations. Our findings enhance understanding adaptation among crops, supporting development more effective management strategies mitigate economic losses hops, mint, other crop production.

Language: Английский

Citations

0
Azoxystrobin-Induced Physiological and Biochemical Alterations in Apis mellifera Workers of Different Ages DOI Creative Commons

Xinle Duan,

Wenlong Tong,

Bowen Tao

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(5), P. 449 - 449

Published: April 24, 2025

Fungal diseases of agricultural crops cause severe economic losses to the growers. For control these diseases, azoxystrobin is one recommended fungicides. This fungicide systemic in action and expected reach floral part treated crop its residue pollen nectar, natural food sources honey bees, which could be collected fed on by thus affecting their health. The purpose this study was determine physiological chemical changes caused bee workers (Apis mellifera L). Workers at 1, 8, 21 days old were with 125, 167, 250 mg/L concentrations for seven days; survival rates, activities carboxylesterase (CarE), glutathione S-transferases (GSTs), cytochrome P450 enzyme (CYP450), catalase (CAT), superoxide dismutase (SOD) enzymes, expression levels immune (Aba, Api, Def1, Hym) nutrition genes (Ilp1, Ilp2, Vg) detected. Our findings revealed that affected workers, particularly 1- 21-day-old who responded stress increased detoxification protective might have costs. Additionally, genes, a decreased trend compared 8-day-old leading reduced resistance external stressors mortality rates. These provide important insights into adverse effects different ages emphasize potential risks colony stability individual recommends an urgent ban such harmful being used fungi agriculture, especially during plant flowering.

Language: Английский

Citations

0
Binding characteristics of pheromone-binding protein 1 in Glyphodes pyloalis to organophosphorus insecticides: Insights from computational and experimental approaches DOI
Yi-Jiangcheng Li,

Fengming Gu,

Hong‐Chao Chen

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 260, P. 129339 - 129339

Published: Jan. 11, 2024

Language: Английский

Citations

3
RNAi silencing CHS1 gene shortens the mortality time of Plutella xylostella feeding Bt‐transgenic Brassica napus DOI

Purong Deng,

Yujia Peng,

Zhilu Sheng

et al.

Pest Management Science, Journal Year: 2024, Volume and Issue: 80(6), P. 2610 - 2618

Published: Jan. 22, 2024

Abstract BACKGROUND Insect‐resistance genetically modified (GM) plants derived from Bacillus thuringiensis (Bt) have been cultivated to control pests, but continuous cultivation of Bt‐transgenic at large‐scale regions leads the resistance evolution target insects transgenic plants. RNA interference (RNAi) technology is considered an effective strategy in delaying insects. RESULTS We here developed a single oilseed rape ( Brassica napus ) line with hairpin chitin‐synthase 1 gene CHS1 Plutella xylostella hpPxCHS1 and pyramid B . harboring Bt Cry1Ac ). Escherichia coli HT115 delivered showed negative effects on growth P The significantly reduced larval weight length increased its lethality rate, down‐regulation expression PxCHS1 CONCLUSION Compared , pyramid‐transgenic shorted mortality time indicating that RNAi synergistic protein improves effectiveness controlling Our results proved can delay © 2024 Society Chemical Industry.

Language: Английский

Citations

2
Comparative genomics provides insights into molecular adaptation to hypermetamorphosis and cantharidin metabolism in blister beetles (Coleoptera: Meloidae) DOI Creative Commons
Alessandra Riccieri, Lucrezia Spagoni, Ming Li

et al.

Integrative Zoology, Journal Year: 2024, Volume and Issue: 19(5), P. 975 - 988

Published: March 15, 2024

Blister beetles (Coleoptera: Meloidae) are currently subdivided into three subfamilies: Eleticinae (a basal group), Nemognathinae, and Meloinae. These all characterized by the endogenous production of defensive terpene cantharidin (CA), whereas two most derived subfamilies show a hypermetamorphic larval development. Here, we provide novel draft genome assemblies five species sampled across blister beetle (Iselma pallidipennis, Stenodera caucasica, Zonitis immaculata, Lydus trimaculatus, Mylabris variabilis) performed comparative analysis with other available Meloidae genomes closely-related canthariphilous (Pyrochroa serraticornis) to disclose adaptations at molecular level. Our results highlighted expansion selection genes potentially responsible for CA metabolism, as well its mobilization vesicular compartmentalization. Furthermore, observed adaptive patterns gain devoted epigenetic regulation, development, morphogenesis, possibly related hypermetamorphosis. We hypothesize that genetic occurred support both biosynthesis hypermetamorphosis, crucial aspects biology likely contributed their evolutionary success.

Language: Английский

Citations

2
Elucidating the detoxification efficacy of Periplaneta americana delta glutathione S-transferase 1 (PaGSTd1) against organophosphates DOI
Hong Sun, Xinyu Li, Xinyue Yuan

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 203, P. 106013 - 106013

Published: July 4, 2024

Language: Английский

Citations

2
Architecture and potential roles of a delta-class glutathione S-transferase in protecting honey bee from agrochemicals DOI Creative Commons
Timothy W. Moural, Sonu Koirala B K, Gaurab Bhattarai

et al.

Chemosphere, Journal Year: 2023, Volume and Issue: 350, P. 141089 - 141089

Published: Dec. 30, 2023

Language: Английский

Citations

5
Microarray and Functional Pathway Analyses Revealed Significantly Elevated Gene Expressions Associated with Metabolic Resistance to Oxamyl (Vydate) in Lygus lineolaris DOI Creative Commons
Yu Cheng Zhu, Yuzhe Du,

Xiaofen Liu

et al.

Toxics, Journal Year: 2024, Volume and Issue: 12(3), P. 188 - 188

Published: Feb. 28, 2024

The tarnished plant bug (TPB, Lygus lineolaris) remains a major pest for variety of crops. Frequent sprays on row crops, especially cotton, prompted resistance development in field populations. To maintain chemical control as an effective tool against the pest, knowledge global gene regulations is desirable better understanding and managing resistance. Novel microarray expressions 6688 genes showed 685 significantly upregulated 1382 downregulated oxamyl-selected TPBs (Vyd1515FF[R]) from cotton field. Among (participated 470 pathways), 176 code 30 different enzymes, 7 participate 24 metabolic pathways. Six important detoxification pathways were controlled by 20 genes, coding 11 esterases, two P450s, oxidases, three pathway-associated enzymes (synthases, reductase, dehydrogenase). Functional analyses substantially enhanced biological processes molecular functions, with hydrolase activity most function (controlled 166 genes). Eleven esterases belong to acting ester bond subclass hydrolases. Surprisingly, only one GST significant upregulation, but it was not involved any pathway. Therefore, this research reports set 6 enzyme classes detoxify carbamate insecticide oxamyl Vyd1515FF. Together previous reports, we have obtained best mechanisms all four conventional economically crop pest. This valuable finding will greatly facilitate tools monitor manage minimize risk environment.

Language: Английский

Citations

1
Comparative Genomics Uncovers the Evolutionary Dynamics of Detoxification and Insecticide Target Genes Across 11 Phlebotomine Sand Flies DOI Creative Commons
Jason Charamis, Sofia Balaska, Panagiotis Ioannidis

et al.

Genome Biology and Evolution, Journal Year: 2024, Volume and Issue: 16(9)

Published: Sept. 1, 2024

Abstract Sand flies infect more than 1 million people annually with Leishmania parasites and other bacterial viral pathogens. Progress in understanding sand fly adaptations to xenobiotics has been hampered by the limited availability of genomic resources. To address this gap, we sequenced, assembled, annotated transcriptomes 11 phlebotomine species. Subsequently, leveraged these resources generate novel evolutionary insights pertaining their xenobiotics, including those contributing insecticide resistance. Specifically, over 2,700 detoxification genes conducted large-scale phylogenetic comparisons uncover dynamics five major gene families: cytochrome P450s (CYPs), glutathione-S-transferases (GSTs), UDP-glycosyltransferases (UGTs), carboxyl/cholinesterases (CCEs), ATP-binding cassette (ABC) transporters. Using comparative approach, show that have evolved diverse CYP GST repertoires, notable lineage-specific expansions groups evolutionarily related known xenobiotic metabolizers. Furthermore, conserved orthologs (i) CYP4G involved cuticular hydrocarbon biosynthesis, (ii) ABCB toxicity, (iii) two primary targets, acetylcholinesterase-1 (Ace1) voltage gated sodium channel (VGSC). The biological produced study provide a foundation for generating testing hypotheses regarding molecular mechanisms underlying xenobiotics.

Language: Английский

Citations

1