Heliyon, Journal Year: 2024, Volume and Issue: 11(1), P. e41340 - e41340
Published: Dec. 19, 2024
Language: Английский
Theranostics, Journal Year: 2024, Volume and Issue: 14(5), P. 2099 - 2126
Published: Jan. 1, 2024
Exosomes, which are small vesicles enclosed by a lipid bilayer and released many cell types, widely dispersed have garnered increased attention in the field of regenerative medicine due to their ability serve as indicators diseases agents with therapeutic potential. Exosomes play crucial role mediating intercellular communication through transfer biomolecules, including proteins, lipids, RNA, other molecular constituents, between cells. The targeted transport proteins nucleic acids specific cells has potential enhance or impair biological functions. applications, they can be used alone combination approaches. examination unique attributes functions these factors emerged prominent study realm biomedical research. This manuscript summarizes origins properties exosomes, structural, biological, physical, chemical aspects. paper offers complete recent progress tissue repair medicine, emphasizing possible implications methods forthcoming regeneration attempts.
Language: Английский
Citations
25
Cell Death Discovery, Journal Year: 2024, Volume and Issue: 10(1)
Published: May 2, 2024
Abstract The escalating threat of bone-related diseases poses a significant challenge to human health. Mesenchymal stem cell (MSC)-derived extracellular vesicles (MSC-EVs), as inherent cell-secreted natural products, have emerged promising treatments for diseases. Leveraging outstanding features such high biocompatibility, low immunogenicity, superior biological barrier penetration, and extended circulating half-life, MSC-EVs serve potent carriers microRNAs (miRNAs), long no-code RNAs (lncRNAs), other biomolecules. These cargo molecules play pivotal roles in orchestrating bone metabolism vascularity through diverse mechanisms, thereby contributing the amelioration Additionally, engineering modifications enhance bone-targeting ability MSC-EVs, mitigating systemic side effects bolstering their clinical translational potential. This review comprehensively explores mechanisms which regulate disease progression. It delves into therapeutic potential adept drug carriers, augmented by engineered modification strategies tailored osteoarthritis (OA), rheumatoid arthritis (RA), osteoporosis, osteosarcoma. In conclusion, exceptional promise exhibited positions them an excellent solution with considerable applications orthopedics.
Language: Английский
Citations
13
Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(19)
Published: March 1, 2024
Abstract Exosomes, nanoparticles secreted by various cells, composed of a bilayer lipid membrane, and containing bioactive substances such as proteins, nucleic acids, metabolites, etc., have been intensively investigated in tissue engineering owing to their high biocompatibility versatile biofunction. However, there is still lack high‐quality review on bone defect regeneration potentiated exosomes. In this review, the biogenesis isolation methods exosomes are first introduced. More importantly, engineered current state knowledge discussed review. Afterward, biomaterial carriers mechanisms repair elucidated compelling evidence presented. Thus, future perspectives concerns revealed help devise advanced modalities based overcome challenges regeneration. It totally believed will attract special attention from clinicians provide promising ideas for works.
Language: Английский
Citations
9
Nano Convergence, Journal Year: 2024, Volume and Issue: 11(1)
Published: June 26, 2024
Abstract Stem cell therapy holds promise for tissue regeneration, yet significant challenges persist. Emerging as a safer and potentially more effective alternative, extracellular vesicles (EVs) derived from stem cells exhibit remarkable abilities to activate critical signaling cascades, thereby facilitating repair. EVs, nano-scale membrane vesicles, mediate intercellular communication by encapsulating diverse cargo of proteins, lipids, nucleic acids. Their therapeutic potential lies in delivering cargos, activating pathways, efficiently mitigating oxidative stress—an essential aspect overcoming limitations cell-based This review focuses on engineering applying EVs emphasizing their role regulating reactive oxygen species (ROS) pathways. Additionally, we explore strategies enhance EV activity, including functionalization incorporation antioxidant defense proteins. Understanding these molecular mechanisms is crucial optimizing EV-based regenerative therapies. Insights into ROS modulation pave the way targeted efficient therapies harnessing EVs.
Language: Английский
Citations
7
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140753 - 140753
Published: Feb. 1, 2025
Language: Английский
Citations
1
Applied Materials Today, Journal Year: 2024, Volume and Issue: 37, P. 102084 - 102084
Published: Feb. 3, 2024
Extracellular vesicles (EVs) are biologically active nanoparticles secreted by cells, which have a variety of physiological functions, such as promoting cell proliferation, regulating immunity, anti-inflammation, and facilitating angiogenesis. However, the application EVs is limited defects low therapeutic efficiency, lack targeting, unstable effects. In recent years, various engineering methods been developed to enhance function EVs. addition, research on isolation mechanisms has also made significant progress. this paper, we review biogenesis, composition, techniques EVs, summarize potential natural well strategies for EV modification, highlight advances in tissue regeneration. Finally, discuss EVs' current problems future perspectives regenerative medicine.
Language: Английский
Citations
6
Frontiers in Molecular Biosciences, Journal Year: 2024, Volume and Issue: 11
Published: June 6, 2024
Skeletal diseases impose a considerable burden on society. The clinical and tissue-engineering therapies applied to alleviate such frequently result in complications are inadequately effective. Research has shifted from conventional based mesenchymal stem cells (MSCs) exosomes derived MSCs. Exosomes natural nanocarriers of endogenous DNA, RNA, proteins, lipids have low immune clearance rate good barrier penetration allow targeted delivery therapeutics. MSC-derived (MSC-exosomes) the characteristics both MSCs exosomes, so they can immunosuppressive tissue-regenerative effects. Despite advances our knowledge MSC-exosomes, their regulatory mechanisms functionalities unclear. Here we review therapeutic potential MSC-exosomes for skeletal diseases.
Language: Английский
Citations
3
Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)
Published: Jan. 11, 2025
Language: Английский
Citations
0
Revista de Osteoporosis y Metabolismo Mineral, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Citations
0
Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)
Published: March 6, 2025
Tumors and injuries often lead to large mandibular defects. Accelerating the osteogenesis of bone defect areas is a major concern in current research. In this study, dental pulp mesenchymal stem cells (DPSCs) were used as seed cells, local pulsatile parathyroid hormone (PTH) delivery system was an osteogenic-inducing active ingredient act on DPSCs osteoblasts, which applied jaw area evaluate its therapeutic effect regeneration. Pulsatile systems, both with without PTH, developed following protocols outlined our previous study. vitro, biocompatibility assessed using Cell Counting Kit-8 (CCK8) assay live/dead cell staining. Osteogenic differentiation evaluated through alkaline phosphatase staining alizarin red vivo, critical defects diameter 10 mm created mandibles white rabbits. The osteogenic further gross observation, X-ray imaging, histological examination. vitro experiments CCK8 assays demonstrated that successfully adhered surface PTH system, showing no significant difference compared control group. Furthermore, Alizarin Red confirmed localized effectively induced into leading secretion abundant calcium nodules. Animal studies revealed promoted DPSCs, facilitating repair rhythmic release from induces DPSCs. By leveraging synergistic interaction between approach facilitates extensive
Language: Английский
Citations
0