Antibacterial Activity of Green Synthesized Silver Nanoparticles Using Lawsonia inermis Against Common Pathogens from Urinary Tract Infection

Applied Biochemistry and Biotechnology, Journal Year: 2023, Volume and Issue: 196(1), P. 85 - 98

Published: April 26, 2023

Abstract New and creative methodologies for the fabrication of silver nanoparticles (Ag-NPs), which are exploited in a wide range consumer items, significant interest. Hence, this research emphasizes biological approach Ag-NPs through Egyptian henna leaves ( Lawsonia inermis Linn.) extracts analysis prepared Ag-NPs. Plant extract components were identified by gas chromatography mass spectrometry (GC-mass). The analyses carried out UV–visible (UV–Vis), X-ray diffraction (XRD), transmission electron microscope (TEM), scanning (SEM), Fourier transform infrared (FTIR) analysis. UV–Vis reveals that have maximum peak at 460 nm visible light. Structural characterization recorded peaks corresponded to Bragg’s diffractions nano-crystal, with average crystallite sizes varying from 28 60 nm. Antibacterial activities examined, it is observed all microorganisms very sensitive biologically synthesized

Language: Английский

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Antimicrobial and anticancer activities of biosynthesized bimetallic silver-zinc oxide nanoparticles (Ag-ZnO NPs) using pomegranate peel extract

Biomass Conversion and Biorefinery, Journal Year: 2023, Volume and Issue: 14(17), P. 20345 - 20357

Published: April 6, 2023

Abstract In the last two decades, nanomaterials have received much attention for treatment of multidrug-resistant microbes that threaten human health. current study, novelty and scientific significance concentrated on biogenic synthesis bimetallic silver-zinc oxide nanoparticles (Ag-ZnO NPs) using pomegranate peel extract (PPE) first time. The new constructed Ag-ZnO NPs possessed synergistic activity at a low concentration to avoid toxicity elevate superior potential. UV-Vis. characterization illustrated were small in size (15.8 nm), which was observed 395.0 nm. SEM image NPs, incorporated with PPE, exhibited uniform NP surfaces clear surface appearance. It can be detected isolated typically as rounded particle across showed brilliant combined stabilized prepared PPE. Results also revealed potential antibacterial toward Escherichia coli , Pseudomonas aeruginosa Bacillus subtilis Staphylococcus aureus Enterococcus faecalis where minimum inhibitory concentrations (MICs) 62.5, 125, 15.62, 250 µg/ml. Likewise, appeared antifungal against Candida albicans Cryptococcus neoformans Aspergillus fumigatus brasiliensis MICs 7.81, 31.25, 62.5 µg/ml, respectively. Moreover, anticancer activities MCF7 Caco2, IC 50 104.9 52.4 Additionally, these are safe use results cytotoxicity Vero normal cell line confirmed 155.1 Overall, time, successfully biosynthesized PPE; also, they had promising antibacterial, antifungal, activities.

Language: Английский

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Silver nanoparticles improve the fungicidal properties of Rhazya stricta decne aqueous extract against plant pathogens

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 14, 2024

Abstract One of the most promising, non-toxic, and biocompatible developments for many biological activities is green synthesis nanoparticles from plants. In this work, we investigated antifungal activity silver (AgNPs) biosynthesized Rhazya stricta aqueous extract against several plant pathogenic fungi. UV–visible spectroscopy, Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), transmitted electron microscopy (TEM) were used to analyze AgNPs. Drechslera halodes, tetramera, Macrophomina phaseolina, Alternaria alternata, Curvularia australiensis tested their activity. Surface Plasmon Resonance (SPR) Aq. AgNPs Alkaline was observed at 405 nm 415 nm, respectively. FTIR analysis indicated hydroxyl, nitrile, amine, ketone functional groups. Alka-line had velocities − 27.7 mV 37.9 sizes 21–90 7.2–25.3 respectively, according zeta studies TEM. The examination revealed that all species' mycelial development significantly inhibited, accompanied by severe ultra-structural alterations. Among treatments, effective fungicide. M. phaseolina statistically resistant, whereas A. alternata vulnerable. To best our knowledge, first report on R. stricta' s these species.

Language: Английский

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Synthesis and characterization of innovative GA@Ag-CuO nanocomposite with potent antimicrobial and anticancer properties

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 3, 2025

Cancer and microbial infections place a significant burden on the world's health systems can increase rate of disease mortality. In current study, novel nanocomposite based Gum Arabic, silver copper oxide nanoparticles (GA@Ag-CuO nanocomposite) was synthesized to overcome problem infection in cancer treatment. Characterization using UV-Vis. spectrophotometer reveals that, observed peak spectrum formed by O.D. at 0.755, confirmed that produced GA@Ag-CuO small discernible 360 nm. The particles' diameters varied from 9.5 nm 49.5 nm, with mean diameter 25.53 ± 1.4 created Arabic filtrate rich active functional groups, provided polydisperse NPs were intended reduce, stabilize, act as capping agents. Based XRD data, crystallized had face-centered (fcc) crystal structure. Biosafety assessed toward Wi 38 normal cell line, where it showed safety tested line IC50 154.2 µg/mL. Antimicrobial results has antibacterial activity MICs 15.6, 125, 31.25 125 µg/mL against S. epidermis, aureus, L. plantrum, typhimurium, respectively. Likewise, antifungal C. albicans neoformans 62.5 15.62 µg/ml, Moreover, displayed promising anticancer 26.11 59.5 µg/ml MCF-7 Hep-G2, conclusion, demonstrated antibacterial, antifungal, activities.

Language: Английский

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An eco-friendly and cost-effective approach for the synthesis of a novel GA@CuO–ZnO nanocomposite: characterization, antimicrobial and anticancer activities

RSC Advances, Journal Year: 2025, Volume and Issue: 15(1), P. 513 - 523

Published: Jan. 1, 2025

In this study, a nanocomposite based on copper oxide–zinc oxide nanoparticles and Gum Arabic (GA@CuO–ZnO nanocomposite) was successfully synthesized using green method for increasing antimicrobial anticancer activities.

Language: Английский

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Antifungal activity of myco-synthesized bimetallic ZnO-CuO nanoparticles against fungal plant pathogen Fusarium oxysporum

Biomass Conversion and Biorefinery, Journal Year: 2023, Volume and Issue: 14(20), P. 25395 - 25409

Published: July 20, 2023

Abstract Fusarium species are considered one of the most destructing plant pathogens. In current study, bimetallic zinc oxide-copper oxide nanoparticles (ZnO-CuO NPs) were myco-synthesized using Aspergillus fumigatus for controlling oxysporum growth. was isolated from soil and identified morphologically genetically. The ZnO-CuO NPs characterized UV-Vis, DLS, HR-TEM, SEM, XRD analyses. HR-TEM characterization method indicated that, biosynthesized appeared as semi-spherical with average diameter specified 54.18 ± 1.9 nm. DLS described characteristic particle size diffusion calculated 85.52 nm, 90.85 92.85 nm ZnO NPs, CuO respectively. Additionally, SEM image displays basic NP surface character exterior impression apparent. separated naturally spherical particles connected within fungal filtrate, which illuminated fused capped filtrate. Antifungal activity evaluated against F. . Results revealed that exhibited promising antifungal toward where inhibition zone at 1000 µg/ml 22.8 0.76 mm, MIC 125 µg/ml. Moreover, growth percentages different concentrations 1000, 500, 250, 88.9, 65.5, 41.1, 8.9% respectively, highest 88.9% concentration µg/ml, while lowest TEM ultrastructure results, treated a clear destruction found in all cell contents disintegration wall well plasma membrane. Also, nucleus small damaged shape chromatin materials distributed several dark stained bodies cytoplasm. conclusion, successfully A. , it had

Language: Английский

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Green Biosynthesis of Zinc Oxide Nanoparticles Using Pluchea indica Leaf Extract: Antimicrobial and Photocatalytic Activities

Molecules, Journal Year: 2023, Volume and Issue: 28(12), P. 4679 - 4679

Published: June 9, 2023

Nanotechnology is playing a critical role in several essential technologies with nanoscale structures (nanoparticles) areas of the environment and biomedicine. In this work, leaf extract Pluchea indica was utilized to biosynthesize zinc oxide nanoparticles (ZnONPs) for first time evaluated antimicrobial photocatalytic activities. Different experimental methods were used characterize biosynthesized ZnONPs. The ZnONPs showed maximum Ultraviolet-visible spectroscopy (UV-vis) absorbance at wavelength 360 nm. X-Ray diffraction (XRD) pattern exhibits seven strong reflection peaks, average particle size 21.9 Fourier-transform infrared (FT-IR) spectrum analysis reveals presence functional groups that help biofabrication. existence Zn O confirmed by Energy-dispersive X-ray (EDX) morphology SEM images. Antimicrobial studies have efficacy against Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, Bacillus subtilis, Staphylococcus aureus, Candida albicans Cryptococcus neoformans where inhibition zones concentration 1000 µg/mL 21.83 ± 0.76, 13.0 1.1, 14.9 0.85, 24.26 17.0 1.0, 20.67 0.57 19.0 1.0 mm respectively. Under both dark sunlight irradiation, activity towards degradation thiazine dye (methylene blue-MB). Approximately 95% MB broken down pH 8 after 150 min exposure. aforementioned results, therefore, suggest synthesized implementing environmentally friendly techniques can be employed variety environmental biomedical applications.

Language: Английский

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Biosynthesis, Characterization, and Antifungal Activity of Novel Trimetallic Copper Oxide–Selenium–Zinc Oxide Nanoparticles against Some Mucorales Fungi

Microorganisms, Journal Year: 2023, Volume and Issue: 11(6), P. 1380 - 1380

Published: May 24, 2023

Metal nanoparticles are assumed to be a new generation of biologically active materials. The integrations between more than one metal synergetic multifunctional features. In the current study, trimetallic copper-selenium-zinc oxide (Tri-CSZ NPs) were successfully mycosynthesized using Aspergillus niger through an ecofriendly method for first time. biosynthesis particles was characterized physiochemical and topographical analysis. analysis included Fourier transform infrared spectroscopy (FTIR), which affirmed that Tri-CSZ NPs relies on functional groups fungal filtrates. Additionally, UV-visible X-ray diffraction patterns proposed formation NPs; moreover, topography confirmed micromorphology similar stick, with ends having tetragonal pyramid shape, average nanosize about 26.3 ± 5.4 nm. Cytotoxicity results reveled have no cytotoxicity human normal cell line Wi 38 at low concentrations, where IC50 521 µg/mL. Furthermore, antifungal activity evaluated. revealed promising against Mucor racemosus, Rhizopus microsporus, Lichtheimia corymbifera, Syncephalastrum racemosum, minimum inhibitory concentrations (MICs) 1.95, 7.81, 62.5, 3.9 µg/mL, fungicidal (MFCs) 250, 125, 1000 respectively. conclusion, A. niger, fungi causing mucormycosis.

Language: Английский

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Aspergillus terreus-Mediated Selenium Nanoparticles and Their Antimicrobial and Photocatalytic Activities

Crystals, Journal Year: 2023, Volume and Issue: 13(3), P. 450 - 450

Published: March 4, 2023

Selenium (Se) is a nutritional component necessary for animal and plant development reproduction. nanoparticles (SeNPs) have high absorption rate during routine supplementation. In the current study, cell-free extract of Aspergillus terreus was used as reducing stabilizing agent in synthesis SeNPs using green eco-friendly method. The mycosynthesized were characterized by UV-visible spectrophotometry, Fourier transform infrared spectroscopy, X-ray diffraction dynamic light scattering, transmission electron microscopy, scanning microscopy. results characterization process showed that had spherical shapes sizes less than 100 nm. Results exhibited promising antibacterial activity against both Gram-positive Gram-negative bacteria where inhibition zones 14, 20, 16, 13 mm toward S. haemolyticus, aureus, E. coli, K. pneumoniae, respectively. However, it weak antifungal C. albicans, zone 12 mm. efficacy decolorization malachite dye investigated. illustrated rapid biodegradation dye, reaching up to 89% after 240 min. conclusion, successfully biosynthesized A. demonstrated antimicrobial photocatalytic activities.

Language: Английский

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Biosynthesis of titanium dioxide nanoparticles using probiotic bacterial strain, Lactobacillus rhamnosus, and evaluate of their biocompatibility and antifungal activity

Biomass Conversion and Biorefinery, Journal Year: 2023, Volume and Issue: 14(19), P. 23961 - 23983

Published: July 26, 2023

Abstract The main goal of the current study is to biosynthesize titanium dioxide nanoparticles (TiO 2 -NPs) using green approach biocontrol different fungal strains isolated from historical Description Egypt book. Nineteen were deteriorated parts and identified by traditional molecular methods as Aspergillus flavus (8-isolates), versicolor (2-isolates), ustus (4-isolates), chinensis Penicillium citrinum chrysogenum (1-isolate). These showed high cellulase, amylase, pectinase, gelatinase activities which have a significant role in biodeterioration. biomass filtrate probiotic strain, Lactobacillus rhamnosus , was used fabricate TiO -NPs characterized UV-Vis, FT-IR, XRD, TEM, SEM, EDX, DLS, zeta potential. obtained data successful formation spherical anatase phase NPs with sizes 3–7 nm potential values – 19.9 36.8 mV. components as-formed nanomaterial Ti O weight percentages 55.91 46.25, respectively. biocompatibility synthesized investigated toward two normal cell lines, WI38 HFB4, reveal low toxicity at concentrations (IC 50 > 300 μg mL –1 ). Therefore, ≤ fungi. Data promising activity various (300, 200, 100 ) inhibit growth varied inhibition zones dose-dependent manner. This exhibited efficacy bacterial synthesis that can be preserve books deterioration. Graphical

Language: Английский

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