Journal of Foot and Ankle Surgery (Asia Pacific), Journal Year: 2024, Volume and Issue: 11(4), P. 161 - 168
Published: Sept. 20, 2024
Language: Английский
Journal of Orthopaedic Translation, Journal Year: 2025, Volume and Issue: 50, P. 333 - 353
Published: Jan. 1, 2025
Bone and soft tissues are connected by a complex interface that is crucial for the smooth transfer of mechanical stress. Effective repair this requires bio-scaffolds specifically designed to support regeneration diverse cell types signalling molecules. With advances in micro- nanotechnologies, gradient biomaterial scaffolds have demonstrated significant potential tissue regeneration. This paper reviews structure bone-soft interface, various scaffold types, construction methods. It also discusses recent developments future directions engineering, emphasizing restore natural function interfaces. Overall, provides valuable insights into application offering inspiration biomimetic approaches soft-hard medical engineering. First, emphasis on could significantly impact clinical practices related integration, ultimately improving patient outcomes quality life. Second, it aligns with growing trend potentially inspiring new innovations strategies.
Language: Английский
Citations
1
Viruses, Journal Year: 2024, Volume and Issue: 16(4), P. 570 - 570
Published: April 7, 2024
Phage display is a versatile method often used in the discovery of peptides that targets disease-related biomarkers. A major advantage this technology ease and cost efficiency affinity selection, also known as biopanning, to identify novel peptides. While it relatively straightforward with optimal binding affinity, pharmacokinetics selected prove be suboptimal. Therefore, careful consideration experimental conditions, including choice using vitro, situ, or vivo selections, essential generating high specificity demonstrate desirable pharmacokinetics. Specifically, combination has been proven influence biodistribution clearance peptide-conjugated nanoparticles. Additionally, marked difference properties between nanoparticles must considered. peptide depends primarily on physiochemical can modified by amino acid modifications, size shape affect both absorption distribution. Thus, optimization desired pharmacokinetic should an important biopanning strategies enable selection effectively target biomarkers vivo.
Language: Английский
Citations
5
Aesthetic Plastic Surgery, Journal Year: 2024, Volume and Issue: unknown
Published: July 29, 2024
Language: Английский
Citations
5
Advanced Materials, Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 9, 2024
Modulating the inflammatory microenvironment to reconstruct fibrocartilaginous layer while promoting tendon repair is crucial for enhancing tendon-to-bone healing in rotator cuff (RCR), a persistent challenge orthopedics. Small extracellular vesicles (sEVs) hold significant potential modulate inflammation, yet efficient production of highly bioactive sEVs remains substantial barrier their clinical application. Moreover, achieving minimally invasive local delivery interface presents technical difficulties. Herein, circadian rhythm adipose-derived stem cells modulated increase yield and enhance regulatory capacity sEVs. Circadian rhythm-regulated (CR-sEVs) cyclic adenosine monophosphate signaling pathway macrophage (Mφ) via platelet factor 4 delivery, thereby inhibiting Mφ M1 polarization. Subsequently, triphasic microneedle (MN) scaffold with tip, stem, base designed CR-sEVs (CR-sEVs/MN) at junction, incorporating tendon-derived decellularized matrix facilitate repair. CR-sEVs/MN mitigates promotes fibrocartilage regeneration, enhances healing, improving biomechanical strength shoulder joint function rat RCR model. Combining this system promising strategy settings.
Language: Английский
Citations
4
British Journal of Hospital Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 19
Published: Jan. 14, 2025
Aims/Background Hypertension (HT) is a prevalent medical condition showing an increasing incidence rate in various populations over recent years. Long-term hypertension increases the risk of occurrence hypertensive nephropathy (HTN), which also health-threatening disorder. Given that very little known about pathogenesis HTN, this study was designed to identify disease biomarkers, enable early diagnosis disease, through utilization high-throughput untargeted metabolomics strategies. Methods The participants were patients admitted Second Affiliated Hospital Chengdu Medical College, Nuclear Industry 416 Hospital, who randomly divided into three groups: Normal group (n = 11), HT 10), and HTN 12). Urine exosomes extracted, purified, subjected analysis. Differential metabolites their significantly enriched Kyoto Encyclopedia Genes Genomes (KEGG) pathways identified. least absolute shrinkage selection operator (LASSO) regression analysis then employed establish diagnostic model for early-stage HTN. Finally, logistic receiver operating characteristic (ROC) curve performed biomarkers related Results Orthogonal partial squares-discriminant (OPLS-DA) revealed significant differences metabolic profiles patient groups. Compared subjects group, groups exhibited upregulated downregulated differential metabolites, respectively. LASSO results indicated 4-hydroxyphenylacetic acid, bilirubin, uracil, iminodiacetic acid are potential or HT. Conclusion With analysis, we successfully identified A further four key namely hold promise
Language: Английский
Citations
0
Orthopedic Research and Reviews, Journal Year: 2025, Volume and Issue: Volume 17, P. 105 - 113
Published: March 1, 2025
Tendon healing after ligament or tendon reconstruction remains a significant challenge. Regenerative tissue engineering, an interdisciplinary field that combines biology, materials science, and offers promising solutions. Recent developments have introduced scaffold designed to enhance the proliferation differentiation of tendon-to-bone cells. These scaffolds possessing three-dimensional composites cells biomaterials, proven effective in facilitating curing post-surgery. The successful development interface is critical factor for early rehabilitation functional recovery. In this mini-review, we present comprehensive update on contemporary strategies synthetic scaffold-based their influence healing. We described compositions, structures features single-layer, multi-layer, gradient with special mechanical properties. examined construction engineering from perspectives biomaterials design strategies, providing evaluation advantages disadvantages associated each approach. Ultimately, review articulates clear research directions aimed at achieving breakthroughs future studies.
Language: Английский
Citations
0
Artificial Cells Nanomedicine and Biotechnology, Journal Year: 2025, Volume and Issue: 53(1), P. 1 - 16
Published: March 10, 2025
Tendon injuries significantly impact quality of life, prompting the exploration innovative solutions beyond conventional surgery. Extracellular Vesicles (EVs) have emerged as a promising strategy to enhance tendon regeneration. In this study, human Stem/Progenitor Cells (TSPCs) were isolated from surgical biopsies and cultured in Growth-Differentiation Factor-5-supplemented medium promote tenogenic differentiation under static dynamic conditions using custom-made perfusion bioreactor. Once at 80% confluence, cells transitioned serum-free for conditioned media collection. Ultracentrifugation revealed presence vesicles with 106 particles/mL concentration sub-200nm diameter size. Dynamic culture yielded 3-fold increase EV protein content compared culture, confirmed by Western-blot analysis. Differences surface marker expression also shown flow cytometric Data suggest that we efficiently developed protocol extracting EVs TSPCs, particularly conditions. This approach enhances content, offering potential therapeutic benefits However, further research is needed fully understand role
Language: Английский
Citations
0
Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 106844 - 106844
Published: March 1, 2025
Language: Английский
Citations
0
International Journal of Molecular Medicine, Journal Year: 2023, Volume and Issue: 52(6)
Published: Nov. 3, 2023
In sports medicine, injuries related to the insertion of tendons into bones, including rotator cuff injuries, anterior cruciate ligament and Achilles tendon ruptures, are commonly observed. However, traditional therapies have proven be insufficient in achieving satisfactory outcomes due intricate anatomical structure associated with these injuries. Adult bone marrow mesenchymal stem cells possess self‑renewal multi‑directional differentiation potential can generate various tissues aid recovery bone, cartilage, adipose tissue hematopoietic tissue. addition, extracellular vesicles derived from known as exosomes, contain lipids, proteins nucleic acids that govern microenvironment, facilitate repair perform biological functions. Studies demonstrated cell‑derived exosomes function natural nanocapsules for drug delivery enhance tendon‑bone healing strength. The present review discusses latest research results on role released by provides valuable information implementing techniques regenerative medicine health.
Language: Английский
Citations
8
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 14
Published: Jan. 8, 2024
This review in sports medicine focuses on the critical role of exosomes managing chronic conditions and enhancing athletic performance. Exosomes, small vesicles produced by various cells, are essential for cellular communication transporting molecules like proteins nucleic acids. Originating from endoplasmic reticulum, they play a vital modulating inflammation tissue repair. Their significance is increasingly recognized, particularly healing injuries, improving articular cartilage lesions, osteoarthritic behavior aiding regeneration. Investigations also highlight their potential boosting performance, especially through myocytes-derived that may enhance adaptability to physical training. Emphasizing multidisciplinary approach, this underlines need thoroughly understand exosome biology, including pathways classifications, fully exploit therapeutic potential. It outlines future directions medicine, focusing personalized treatments, clinical evaluations, embracing technological advancements. research represents frontier using improve athletes’ health performance capabilities.
Language: Английский
Citations
2