A mini review on green nanotechnology and its development in biological effects

Archives of Microbiology, Journal Year: 2023, Volume and Issue: 205(4)

Published: March 22, 2023

The utilization of living organisms for the creation inorganic nanoscale particles is a potential new development in realm biotechnology. An essential milestone nanotechnology process creating dependable and environmentally acceptable metallic nanoparticles. Due to its increasing popularity ease, use ambient biological resources quickly becoming more significant this field study. phrase "green nanotechnology" has gained lot attention refers variety procedures that eliminate or do away with hazardous compounds repair environment. Green nanomaterials can be used biotechnological sectors such as medicine biology, well food textile industries, wastewater treatment agriculture field. construction an updated level knowledge study systems might support revolutionize nanoparticles (NPs) are presented article.

Language: Английский

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Potential of biosynthesized zinc oxide nanoparticles to control Fusarium wilt disease in eggplant (Solanum melongena) and promote plant growth

BioMetals, Journal Year: 2022, Volume and Issue: 35(3), P. 601 - 616

Published: March 31, 2022

In this study, a novel, non-toxic, eco-friendly zinc oxide nanoparticles (ZnO-NPs) was used instead of the synthetic fungicides widely to control destructive phytopathogenic fungus Fusarium oxysporum, causative agent wilt disease in Solanum melongena L. Herein, biosynthesized ZnO-NPs carried out by Penicillium expansum ATCC 7861. vitro, mycosynthesized exhibited antifungal activity against oxysporum. vivo, suppressed cultivated decreasing severity with 75% plant protection. Moreover, stimulated recovery eggplant as an indicated improving morphological and metabolic indicators including height(152.5%), root length(106.6%), fresh biomass (146%), chlorophyll (102.8%), b (67.86%), total soluble carbohydrates (48.5%), protein (81.8%), phenol (10.5%), antioxidant isozymes compared infected control. Therefore, study suggests using alternative not only eradicate (Solanum melongena) but also promote growth parameters aspects.

Language: Английский

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Potential Antimicrobial and Antibiofilm Properties of Copper Oxide Nanoparticles: Time-Kill Kinetic Essay and Ultrastructure of Pathogenic Bacterial Cells

Applied Biochemistry and Biotechnology, Journal Year: 2022, Volume and Issue: 195(1), P. 467 - 485

Published: Sept. 10, 2022

Abstract Mycosynthesis of nanoparticle (NP) production is a potential ecofriendly technology for large scale production. In the present study, copper oxide nanoparticles (CuONPs) have been synthesized from live cell filtrate fungus Penicillium chrysogenum . The created CuONPs were characterized via several techniques, namely Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), transmission electron microscope (TEM), scanning (SEM), and energy-dispersive spectroscopy (EDX). Furthermore, biosynthesized performed against biofilm forming Klebsiella oxytoca ATCC 51,983, Escherichia coli 35,218, Staphylococcus aureus 25,923, Bacillus cereus 11,778. anti-bacterial activity result was shown with zone inhibition determined to be 14 ± 0.31 mm, 16 0.53 11 0.57 10 mm respectively. more susceptible minimal inhibitory concentration (MIC) values 6.25 3.12 µg/mL, respectively, while , MIC value 12.5 25 μg/mL, minimum (MBIC) evident, that excellent anti-biofilm at sub-MIC levels reducing formation by 49% 59% results indicated MBIC on higher than 200 μg/mL 256 suggesting these could not inhibit mature formatted in vitro. Overall, all clearly confirmed ability prepared offer smart approach biomedical therapy resistant microorganisms because its promoted antimicrobial action, but only specified purposes.

Language: Английский

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Synthesis of Silver Nanocomposite Based on Carboxymethyl Cellulose: Antibacterial, Antifungal and Anticancer Activities

Polymers, Journal Year: 2022, Volume and Issue: 14(16), P. 3352 - 3352

Published: Aug. 17, 2022

Traditional cancer treatments include surgery, radiation, and chemotherapy. According to medical sources, chemotherapy is still the primary method for curing or treating today has been a major contributor recent decline in mortality. Nanocomposites based on polymers metal nanoparticles have recently received attention of researchers. In current study, nanocomposite was fabricated carboxymethyl cellulose silver (CMC-AgNPs) their antibacterial, antifungal, anticancer activities were evaluated. The antibacterial results revealed that CMC-AgNPs promising activity against Gram-negative (Klebsiella oxytoca Escherichia coli) Gram-positive bacteria (Bacillus cereus Staphylococcus aureus). Moreover, exhibited antifungal filamentous fungi such as Aspergillus fumigatus, A. niger, terreus. Concerning HepG2 hepatocellular cell line, lowest IC50 values (7.9 ± 0.41 µg/mL) recorded CMC-AgNPs, suggesting strong cytotoxic effect liver cells. As result, our findings suggest antitumor these CMC-Ag due induction apoptosis necrosis hepatic cells via increased caspase-8 -9 diminished levels VEGFR-2. conclusion, activities, which can be used pharmaceutical fields.

Language: Английский

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Biosynthesis of Silver and Gold Nanoparticles and Their Efficacy Towards Antibacterial, Antibiofilm, Cytotoxicity, and Antioxidant Activities

Applied Biochemistry and Biotechnology, Journal Year: 2022, Volume and Issue: 195(2), P. 1158 - 1183

Published: Nov. 7, 2022

The World Health Organization (WHO) reports that the emergence of multidrug-resistant and slow advent novel more potent antitumor antimicrobial chemotherapeutics continue to be highest concern for human health. Additionally, stability, low solubility, negative effects existing drugs make them ineffective. Studies into alternative tactics tackle such tenacious diseases was sparked by anticancer antibacterial. Silver (Ag) gold (Au) nanoparticles (NPs) were created from Trichoderma saturnisporum, much productive fungal strain. Functional extracellular enzymes proteins carried out activities synthesis capping generated nano-metals. Characterization done on obtained Ag-NPs Au-NPs through UV-vis, FTIR, XRD, TEM, SEM. versus methicillin-sensitive Staphylococcus aureus (MSSA) methicillin-resistant (MRSA), Pseudomonas aeruginosa, Klebsiella pneumoniae, antibacterial assessed. In particular, effective against pathogenic bacteria than Au-NPs. Furthermore, antibiofilm study shown had activity Ag-NPs. Interestingly, applying DPPH procedure, these noble metallic NPs antioxidant activity, in which IC50 73.5 μg/mL 190.0 μg/mL, respectively. According cytotoxicity evaluation results, alteration cells as loss their typical shape, partial or complete monolayer, granulation, shrinking, cell rounding with normal Vero 693.68 661.24 Au-NPs, While cancer (Mcf7) 370.56 394.79 produced via green have potential employed medical industry beneficial nanocompounds.

Language: Английский

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Green Biosynthesis of Selenium Nanoparticles Using Orange Peel Waste: Characterization, Antibacterial and Antibiofilm Activities against Multidrug-Resistant Bacteria

Life, Journal Year: 2022, Volume and Issue: 12(6), P. 893 - 893

Published: June 15, 2022

There is an increase of pathogenic multidrug-resistant bacteria globally due to the misuse antibiotics. Recently, more scientists used metal nanoparticles counteract antibacterial resistance. In this study, orange peel waste (OPW) was for selenium nanoparticles' (Se-NPs) biosynthesis through green and ecofriendly method, their applications as antibiofilm agents. Green biosynthesized Se-NPs were characterized using FTIR, XRD, SEM, EDAX, TEM. Characterization results revealed that highly crystalline, spherical, polydisperse, had sizes in range 16-95 nm. The evaluated activities against bacteria. Results illustrated exhibited potential activity Gram-positive (S. aureus ATCC 29213 biofilm-producing clinical isolates S. aureus) Gram-negative (Pseudomonas aeruginosa PAO1, MDR, biofilm, quorum-sensing producing MDR P. aeruginosa, E. coli, K. pneumonia). Moreover, most sensitive at 1000 µg/mL, where inhibition zone 35 mm MIC 25 µg/mL. Furthermore, 0.25 0.5 decreased biofilm significantly. largest noticed pneumonia, which 62% 92% MIC, respectively. conclusion, successfully OPW method promising bacteria, can be later fighting resistant

Language: Английский

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Multifunctional Silver Nanoparticles Based on Chitosan: Antibacterial, Antibiofilm, Antifungal, Antioxidant, and Wound-Healing Activities

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(6), P. 612 - 612

Published: June 8, 2022

The purpose of this study is to create chitosan-stabilized silver nanoparticles (Chi/Ag-NPs) and determine whether they were cytotoxic also their characteristic antibacterial, antibiofilm, wound healing activities. Recently, the development an efficient environmentally friendly method for synthesizing metal based on polysaccharides has attracted a lot interest in field nanotechnology. Colloidal Chi/Ag-NPs are prepared by chemical reduction ions presence Chi, giving Chi/Ag-NPs. Physiochemical properties determined Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), scanning with energy-dispersive (SEM-EDX) analyses. TEM pictures indicate that generated nearly spherical shape thin chitosan covering around Ag core had sizes range 9-65 nm. In vitro antibacterial activity was evaluated against Staphylococcus aureus Pseudomonas aeruginosa resazurin-mediated microtiter plate assay. highest observed lowest concentration Chi/Ag-NPs, which 12.5 µg/mL both bacterial strains. Additionally, showed promising antifungal features Candida albicans, Aspergillus fumigatus, terreus, niger, where inhibition zones 22, 29, 20, 17 mm, respectively. Likewise, revealed potential antioxidant 92, 90, 75% at concentrations 4000, 2000, 1000 µg/mL, IC50 261 µg/mL. Wound results illustrated fibroblasts advanced toward opening close scratch roughly 50.5% after 24-h exposure greatly accelerating process. conclusion, nanocomposite AgNPs successfully exhibited antifungal, antioxidant, activities can be used medical field.

Language: Английский

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Ecofriendly synthesis of silver nanoparticles using Kei-apple (Dovyalis caffra) fruit and their efficacy against cancer cells and clinical pathogenic microorganisms

Arabian Journal of Chemistry, Journal Year: 2022, Volume and Issue: 15(7), P. 103927 - 103927

Published: April 21, 2022

Green fabrication has become a safe approach for producing nanoparticles. Plant-based biogenic synthesis of silver nanoparticles (AgNPs) emerged as possible alternative to traditional chemical production. In this paper, we provide low-cost, green AgNPs utilizing using Kei-apple (Dovyalis caffra) fruit extract. Ultraviolet–visible (UV–Vis) spectroscopy, Fourier Transform Infrared (FTIR), Transmission Electron Microscopy (TEM), X-Ray Diffraction (XRD), Scanning-Electron Microscope (SEM), and Dynamic Light Scattering (DLS) analyses were used characterize produced AgNPs. The formation was shown have surface resonance peak 415 nm in UV–visible spectra, FTIR spectra verified the participation biological molecules Synthesis TEM revealed that biosynthesized mostly spherical form, with size range 12–53 nm. XRD diffractogram demonstrate face cubic centre (fcc) character Excellent anticancer activity recorded where more than 80% Prostate Cancer (PC-3) cell lines inhibited by 100–150 µg/mL AgNPs, while 38% only AgNO3 55.62% D. caffra extract at 150 µg/mL. Destructions PC-3 observed result exposed followed extract, minor alterations AgNO3. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging three fold 100 indicating good antioxidant activity. inhibitory against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans Aspergillus fumigatus inhibition diameter zone 28.22 ± 0.25 mm, 23.21 0.35 27.25 0.03 28.40 0.15 29.23 0.44 9.52 0.5 respectively compared fruits considered promising source multi-biological functions.

Language: Английский

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Antibacterial Activity of Green Synthesized Silver Nanoparticles Using Lawsonia inermis Against Common Pathogens from Urinary Tract Infection

Applied Biochemistry and Biotechnology, Journal Year: 2023, Volume and Issue: 196(1), P. 85 - 98

Published: April 26, 2023

Abstract New and creative methodologies for the fabrication of silver nanoparticles (Ag-NPs), which are exploited in a wide range consumer items, significant interest. Hence, this research emphasizes biological approach Ag-NPs through Egyptian henna leaves ( Lawsonia inermis Linn.) extracts analysis prepared Ag-NPs. Plant extract components were identified by gas chromatography mass spectrometry (GC-mass). The analyses carried out UV–visible (UV–Vis), X-ray diffraction (XRD), transmission electron microscope (TEM), scanning (SEM), Fourier transform infrared (FTIR) analysis. UV–Vis reveals that have maximum peak at 460 nm visible light. Structural characterization recorded peaks corresponded to Bragg’s diffractions nano-crystal, with average crystallite sizes varying from 28 60 nm. Antibacterial activities examined, it is observed all microorganisms very sensitive biologically synthesized

Language: Английский

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Synthesis of Chitosan-Based Gold Nanoparticles: Antimicrobial and Wound-Healing Activities

Polymers, Journal Year: 2022, Volume and Issue: 14(11), P. 2293 - 2293

Published: June 5, 2022

The global spread of multidrug-resistant bacteria has become a significant hazard to public health, and more effective antibacterial agents are required. Therefore, this study describes the preparation, characterization, evaluation gold nanoparticles modified with chitosan (Chi/AuNPs) as reducing stabilizing agent efficient antimicrobial effects. In recent years, development an ecofriendly method for synthesizing metal attracted lot interest in field nanotechnology. Colloidal (AuNPs) were prepared by chemical reduction ions presence (Chi), giving Chi/AuNPs. characterization Chi/AuNPs was carried out transmission electron microscopy (TEM), scanning (SEM), Fourier-transform infrared (FTIR), X-ray diffraction (XRD). appeared spherical monodispersed, diameter ranging between 20 120 nm. synergistic effects AuNPs Chi led disruption bacterial membranes. maximum inhibitory impact seen against P. aeruginosa at 500 µg/mL, zone inhibition 26 ± 1.8 mm, whereas least effect reported S. aureus, 16 2.1 mm highest dose tested. Moreover, exhibited antifungal activity toward Candida albicans when MIC 62.5 µg/mL. Cell viability proliferation developed nanocomposite evaluated using sulphorhodamine B (SRB) assay half concentration (IC50) 111.1 vitro wound-healing model revealed that Chi/AuNP dressing provides relatively rapid efficacious ability, making obtained promising candidate improved bandage materials.

Language: Английский

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