The Frq–Frh Complex Light-Dependently Delays Sfl1-Induced Microsclerotia Formation in Verticillium dahliae DOI Creative Commons

Alexandra Nagel,

Miriam Leonard,

Isabel Maurus

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(7), P. 725 - 725

Published: July 4, 2023

The vascular plant pathogenic fungus Verticillium dahliae has to adapt environmental changes outside and inside its host. V. harbors homologs of Neurospora crassa clock genes. molecular functions interactions Frequency (Frq) Frq-interacting RNA helicase (Frh) in controlling conidia or microsclerotia development were investigated JR2. Fungal mutant strains carrying gene deletions, an FRH point mutation, GFP fusions analyzed on transcript, protein, phenotypic levels as well pathogenicity assays tomato plants. Our results support that the Frq–Frh complex is formed it promotes conidiation, but also suppresses therefore delays formation response light. We a possible link between negative element Frq positive regulator Suppressor flocculation 1 (Sfl1) elucidate regulatory mechanism. Both Sfl1 are mainly present during onset with decreasing protein further development. Induction requires can be delayed at early time points light through complex. Gaining knowledge will improve control fungal growth wilt disease.

Language: Английский

Circadian oscillations in Trichoderma atroviride and the role of core clock components in secondary metabolism, development, and mycoparasitism against the phytopathogen Botrytis cinerea DOI Creative Commons

Marlene Henríquez-Urrutia,

Rebecca Spanner, Consuelo Olivares-Yañez

et al.

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Aug. 11, 2022

Circadian clocks are important for an individual’s fitness, and recent studies have underlined their role in the outcome of biological interactions. However, relevance circadian fungal–fungal interactions remains largely unexplored. We sought to characterize a functional clock biocontrol agent Trichoderma atroviride assess its importance mycoparasitic interaction against phytopathogen Botrytis cinerea . Thus, we confirmed existence rhythms T. , which temperature-compensated modulated by environmental cues such as light temperature. Nevertheless, presence molecular appears be highly dependent on nutritional composition media. Complementation null ( Δfrq ) Neurospora crassa strain with -negative component tafrq restored core function, same period observed latter fungus, confirming bona fide component. Confrontation assays between wild-type mutant strains B. constant or darkness, revealed inhibitory effect atroviride’s capabilities. Interestingly, when confrontation were performed under light/dark cycles, atroviride’ s overgrowth capacity was enhanced inoculations at dawn compared dusk. Deleting clock-negative element FRQ but not vital daily differential phenotype, suggesting that has more significant influence result this interaction. Additionally, components modulate development secondary metabolism including rhythmic production distinct volatile organic compounds (VOCs). study provides evidence how impact diverse aspects lifestyle changes fungal dynamics.

Language: Английский

Citations

17
Circadian clock is critical for fungal pathogenesis by regulating zinc starvation response and secondary metabolism DOI Creative Commons

Qiaojia Lu,

Muqun Yu,

Xianyun Sun

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(13)

Published: March 28, 2025

Circadian clocks are known to modulate host immune responses pathogen infections, yet their role in influencing pathogenesis remains unclear. Here, we investigated the of circadian regulating fungal Fusarium oxysporum , which has multiple genes homologous Neurospora crassa frq due gene duplication events, with Fofrq1 being primary clock gene. The F. plants is controlled by its clock, infections causing severe disease symptoms at dawn. Notably, disruption dramatically reduces pathogenicity. regulate rhythmic expression several transcription factors, including FoZafA, enables adapt zinc starvation within plant, and FoCzf1, governs production toxin fusaric acid. Together, our findings highlight critical roles pathogenicity response secondary metabolite production.

Language: Английский

Citations

0
Comparative Roles of Rad4A and Rad4B in Photoprotection of Beauveria bassiana from Solar Ultraviolet Damage DOI Creative Commons
Lei Yu, Siyuan Xu,

Xin-Cheng Luo

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(2), P. 154 - 154

Published: Jan. 23, 2023

The Rad4-Rad23-Rad33 complex plays an essential anti-ultraviolet (UV) role depending on nucleotide excision repair (NER) in budding yeast but has been rarely studied filamentous fungi, which possess two Rad4 paralogs (Rad4A/B) and orthologous Rad23 rely the photorepair of UV-induced DNA lesions, a distinct mechanism behind photoreactivation UV-impaired cells. Previously, nucleocytoplasmic shuttling proved to be highly efficient conidia inactivated by UVB, major component solar UV, due its interaction with Phr2 Beauveria bassiana, wide-spectrum insect mycopathogen lacking Rad33. Here, either Rad4A or Rad4B was proven localize exclusively nucleus interact Rad23, previously shown white collar protein WC2 as regulator photorepair-required photolyases (Phr1 Phr2) B. bassiana. Δrad4A mutant lost ~80% conidial UVB resistance ~50% activity UVB-inactivated 5 h light exposure. Intriguingly, reactivation rates UVB-impaired were observable only presence rad4A after dark incubation exceeding 24 h, implicating extant, infeasible, NER for field where night (dark) time is too short. Aside from strong anti-UVB role, played no other bassiana's lifecycle while functionally redundant. Our findings uncover that depends ascribed linked expands molecular basis underlying fungal adaptation UV irradiation Earth's surface.

Language: Английский

Citations

8
Co-Regulatory Roles of WC1 and WC2 in Asexual Development and Photoreactivation of Beauveria bassiana DOI Creative Commons
Siyuan Xu, Lei Yu,

Xin-Cheng Luo

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(3), P. 290 - 290

Published: Feb. 23, 2023

The white collar proteins WC1 and WC2 interact with each other to form a complex acting as well-known transcription regulator required for the operation of circadian clock in Neurospora, but their roles insect-pathogenic fungal lifecycles remain poorly understood. Here, we report that orthologs co-regulate conidiation capacity conidial resistance solar ultraviolet-B (UVB) irradiation Beauveria bassiana, after high activities photorepair UVB-induced DNA damages were elucidated previously insect mycopathogen, which features non-rhythmic capacity. yield, UVB resistance, photoreactivation rate UVB-impaired conidia greatly reduced null mutants wc1 wc2 compared control strains. However, many lifecycle-related phenotypes, except antioxidant response, rarely affected two mutants. Transcriptomic analysis revealed largely overlapping regulating gene networks. Most differentially expressed genes identified from (1380) (1001) co-downregulated (536) or co-upregulated (256) at similar levels, including several aerial photorepair. These findings expand molecular basis underlying adaptation UV offer novel insight into genome-wide co-regulatory B. bassiana's asexual development vivo against damage.

Language: Английский

Citations

8
Rad1 and Rad10 Tied to Photolyase Regulators Protect Insecticidal Fungal Cells from Solar UV Damage by Photoreactivation DOI Creative Commons
Lei Yu, Siyuan Xu,

Xin-Cheng Luo

et al.

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(11), P. 1124 - 1124

Published: Oct. 25, 2022

Beauveria bassiana serves as a main source of global fungal insecticides, which are based on the active ingredient formulated conidia vulnerable to solar ultraviolet (UV) irradiation and restrained for all-weather application in green agriculture. The anti-UV proteins Rad1 Rad10 required nucleotide excision repair (NER) UV-injured DNA model yeast, but their roles remain rarely exploredin filamentous fungi. Here, orthologues that accumulated more nuclei than cytoplasm B. proved capable reactivating UVB-impaired or UVB-inactivated efficiently by 5h light exposure incapable doing so 24 h dark incubation if UVB was lethal. Each orthologue found interacting with other two white collar (WC1 WC2), be regulators photolyases (Phr1 Phr2) individually efficient photorepair UVB-induced lesions either photolyase alone. photoreactivation activity far compromised when protein-protein interactions were abolished absence Phr1 Phr2 lost function. detected suggest direct links regulators. In bassiana, therefore, tied have high activities photoprotection from UV damage insufficient NER field, where night (dark) time is too short, no lifecycle vitro vivo.

Language: Английский

Citations

11
High photoreactivation activities of Rad2 and Rad14 in recovering insecticidal Beauveria bassiana from solar UV damage DOI
Lei Yu, Siyuan Xu,

Xin-Cheng Luo

et al.

Journal of Photochemistry and Photobiology B Biology, Journal Year: 2024, Volume and Issue: 251, P. 112849 - 112849

Published: Jan. 23, 2024

Language: Английский

Citations

2
Rad2, Rad14 and Rad26 recover Metarhizium robertsii from solar UV damage through photoreactivation in vivo DOI
Peng Han,

Yilu Zhang,

Sheng‐Hua Ying

et al.

Microbiological Research, Journal Year: 2023, Volume and Issue: 280, P. 127589 - 127589

Published: Dec. 23, 2023

Language: Английский

Citations

5
Efficient Photoreactivation of Solar UV‐InjuredMetarhizium robertsii by Rad1 and Rad10 Linked to DNA Photorepair‐Required Proteins DOI

Yi‐Lu Zhang,

Peng Han, Sheng‐Hua Ying

et al.

Photochemistry and Photobiology, Journal Year: 2022, Volume and Issue: 99(4), P. 1122 - 1130

Published: Nov. 28, 2022

Abstract Nucleotide excision repair (NER) of ultraviolet (UV)‐induced DNA lesions known as cyclobutane pyrimidine dimer (CPD) and (6–4)‐pyrimidine‐pyrimidone (6‐4PP) photoproducts depends on the activities multiple anti‐UV radiation (RAD) proteins in budding yeast. However, NER remains poorly filamentous fungi, whose are photorepaired by one or two photolyases, namely CPD‐specific Phr1 and/or 6‐4PP‐specific Phr2. Previously, white collar WC1 WC2 were proven to regulate expressions phr2 phr1 photorepair 6‐4PP CDP lesions, respectively, Metarhizium robertsii , a entomopathogenic‐phytoendophytic fungus. We report here high orthologous Rad1 Rad10 5‐h photoreactivation UVB‐injured UVB‐inactivated conidia but severely compromised capability their reactivating those via 24‐h dark incubation M. . The null mutants rad1 rad10 much more conidial UVB resistance than previous wc1 wc2 Multiple protein–protein (Rad1‐Rad10, Rad1‐WC2, Rad10‐Phr1, WC1‐Phr1/2 WC2‐Phr1/2) interactions detected suggest direct/indirect links Phr1/2 WC1/2 an importance for activities. Conclusively, photoreactivate UVB‐impaired through with photorepair‐required proteins.

Language: Английский

Citations

7
The Frq–Frh Complex Light-Dependently Delays Sfl1-Induced Microsclerotia Formation in Verticillium dahliae DOI Creative Commons

Alexandra Nagel,

Miriam Leonard,

Isabel Maurus

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(7), P. 725 - 725

Published: July 4, 2023

The vascular plant pathogenic fungus Verticillium dahliae has to adapt environmental changes outside and inside its host. V. harbors homologs of Neurospora crassa clock genes. molecular functions interactions Frequency (Frq) Frq-interacting RNA helicase (Frh) in controlling conidia or microsclerotia development were investigated JR2. Fungal mutant strains carrying gene deletions, an FRH point mutation, GFP fusions analyzed on transcript, protein, phenotypic levels as well pathogenicity assays tomato plants. Our results support that the Frq–Frh complex is formed it promotes conidiation, but also suppresses therefore delays formation response light. We a possible link between negative element Frq positive regulator Suppressor flocculation 1 (Sfl1) elucidate regulatory mechanism. Both Sfl1 are mainly present during onset with decreasing protein further development. Induction requires can be delayed at early time points light through complex. Gaining knowledge will improve control fungal growth wilt disease.

Language: Английский

Citations

2