Journal of Experimental Biology and Agricultural Sciences,
Journal Year:
2024,
Volume and Issue:
12(3), P. 446 - 456
Published: July 15, 2024
Recently,
the
advancement
in
sustainable
methods
for
fabricating
novel
metabolites
is
one
of
prime
challenges
metabolic
engineering.
The
current
increase
fuel
prices
and
its
limited
supply
made
scientific
community
more
concerned
about
finding
an
alternate
source
generation.
Scientists
are
now
interested
biofuel
because
low
cost
ease
production.
An
intriguing
area
research
engineering
using
imaginative
manipulation
microbes
to
manufacture
chemicals
or
molecules
commercial
importance.
One
such
bacterium
whose
potential
rapidly
attracting
attention
fraternity
Cyanobacteria,
which
either
single-celled
multi-cellular
filamentous
photosynthetic
organisms
that
can
also
fix
CO2.
generation
has
been
transformed
by
use
CRISPR
(clustered
regularly
interspaced
short
palindromic
repeats)
technology
cyanobacteria,
allows
precise
genetic
alterations
improve
their
processes.
effectively
modify
cyanobacterial
genome
lipid
accumulation,
maximize
efficiency,
enhance
stress
tolerance.
Cyanobacteria
have
gained
as
a
production
due
several
advantageous
characteristics
like
capacity,
manipulation,
lack
dependency
on
fertile
land,
high
biomass
yield,
versatile
etc.
our
present
manuscript
aims
catalogue.
play
pivotal
role
developing
environmentally
friendly
energy
solutions
converting
CO2
into
renewable
sources,
serving
flexible
platform
producing
different
types
biofuels
reducing
greenhouse
gas
emissions.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 7, 2023
Abstract
The
overexpression
of
genes
frequently
arises
in
Nakaseomyces
(formerly
Candida
)
glabrata
via
gain-of-function
mutations,
gene
duplication
or
aneuploidies,
with
important
consequences
on
pathogenesis
traits
and
antifungal
drug
resistance.
This
highlights
the
need
to
develop
specific
genetic
tools
mimic
study
amplification
this
fungal
pathogen.
Here,
we
report
development,
validation,
applications
first
CRISPR
activation
(CRISPRa)
system
N.
for
targeted
overexpression.
Using
system,
demonstrate
ability
CRISPRa
drive
high
levels
expression
,
further
assess
optimal
guide
RNA
targeting
robust
We
overexpress
involved
resistance,
detect
corresponding
phenotypic
alterations
these
key
traits,
including
characterization
novel
phenotypes.
Finally,
capture
strain
variation
using
our
two
commonly
used
backgrounds.
Together,
tool
will
expand
capacity
functional
pathogen,
numerous
possibilities
future
applications.
Journal of Fungi,
Journal Year:
2023,
Volume and Issue:
9(11), P. 1093 - 1093
Published: Nov. 9, 2023
Microsphaeropsis,
taxonomically
classified
within
the
kingdom
fungi,
phylum
Ascomycota,
subphylum
Deuteromycotina,
class
Coelomycetes,
order
Sphaeropsidales,
and
family
Sphaeropsidaceae,
exhibit
a
ubiquitous
distribution
across
various
geographical
regions.
These
fungi
are
known
for
their
production
of
secondary
metabolites,
characterized
by
both
structural
novelty
potent
biological
activity.
Consequently,
they
represent
significant
reservoir
advancement
novel
pharmaceuticals.
In
this
paper,
systematic
review
was
present,
marking
analysis
metabolites
synthesized
Microsphaeropsis
reported
between
1980
2023.
A
total
112
compounds,
comprising
polyketones,
macrolides,
terpenoids,
nitrogen-containing
were
from
Microsphaeropsis.
Remarkably,
among
these
49
discoveries,
contribution
to
field.
concise
summary
diverse
activities
provided,
including
antibacterial,
antitumor,
antiviral
properties
other
bioactivities.
This
stands
as
valuable
reference,
poised
guide
further
investigations
into
active
natural
products
derived
potential
contributions
development
medicinal
resources.
Journal of Experimental Biology and Agricultural Sciences,
Journal Year:
2024,
Volume and Issue:
12(3), P. 446 - 456
Published: July 15, 2024
Recently,
the
advancement
in
sustainable
methods
for
fabricating
novel
metabolites
is
one
of
prime
challenges
metabolic
engineering.
The
current
increase
fuel
prices
and
its
limited
supply
made
scientific
community
more
concerned
about
finding
an
alternate
source
generation.
Scientists
are
now
interested
biofuel
because
low
cost
ease
production.
An
intriguing
area
research
engineering
using
imaginative
manipulation
microbes
to
manufacture
chemicals
or
molecules
commercial
importance.
One
such
bacterium
whose
potential
rapidly
attracting
attention
fraternity
Cyanobacteria,
which
either
single-celled
multi-cellular
filamentous
photosynthetic
organisms
that
can
also
fix
CO2.
generation
has
been
transformed
by
use
CRISPR
(clustered
regularly
interspaced
short
palindromic
repeats)
technology
cyanobacteria,
allows
precise
genetic
alterations
improve
their
processes.
effectively
modify
cyanobacterial
genome
lipid
accumulation,
maximize
efficiency,
enhance
stress
tolerance.
Cyanobacteria
have
gained
as
a
production
due
several
advantageous
characteristics
like
capacity,
manipulation,
lack
dependency
on
fertile
land,
high
biomass
yield,
versatile
etc.
our
present
manuscript
aims
catalogue.
play
pivotal
role
developing
environmentally
friendly
energy
solutions
converting
CO2
into
renewable
sources,
serving
flexible
platform
producing
different
types
biofuels
reducing
greenhouse
gas
emissions.
