Non-coding RNAs in disease: from mechanisms to therapeutics

Nature Reviews Genetics, Journal Year: 2023, Volume and Issue: 25(3), P. 211 - 232

Published: Nov. 15, 2023

Language: Английский

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Circulating microRNA signatures associated with disease severity and outcome in COVID-19 patients

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: Aug. 11, 2022

SARS-CoV-2 induces a spectrum of clinical conditions ranging from asymptomatic infection to life threatening severe disease. Host microRNAs have been involved in the cytokine storm driven by and proposed as candidate biomarkers for COVID-19.

Language: Английский

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Oxidative Biomarkers Associated with the Pulmonary Manifestation of Post-COVID-19 Complications

Journal of Clinical Medicine, Journal Year: 2023, Volume and Issue: 12(13), P. 4253 - 4253

Published: June 25, 2023

The role of mitochondria in post coronavirus disease 2019 (post-COVID-19) complications is unclear, especially the long-term pulmonary complications. This study aims to investigate association between post-COVID-19 and mitochondrial regulatory proteins context oxidative stress.Patients who had recovered from COVID-19 were enrolled. According evidence persistent interstitial lung lesions on computed tomography (CT), patients divided into a group (P(+)) control without (P(-)). We randomly selected 80 for investigation (40 subjects each group). Biomarkers levels determined by enzyme-linked immunosorbent assay (ELISA).The serum concentrations significantly higher P(+) group, including PTEN-induced kinase 1 (PINK1): 1.62 [1.02-2.29] ng/mL vs. 1.34 [0.94-1.74] (p = 0.046); Dynamin-1-like protein (DNM1L): 1.6 [0.9-2.4] IQR 0.9 [0.5-1.6] 0.004); Mitofusin-2 (MFN2): 0.3 [0.2-0.5] 0.2 [0.1-0.3] 0.001). Patients also chemokine ligand 18 (PARC, CCL18), IL-6, tumour necrosis factor-alpha (TNF-α) cytokines than P(-) group. concentration interferon alpha (IFN-α) was decreased Furthermore, we observed statistically significant correlations advanced glycation end product (sRAGE) TNF-α (Pearson's factor R 0.637; p < 0.001) DNM1L IFN-α 0.501; 0.002) patients.Elevated biomarkers with indicate their possible pathobiology sequelae. Oxidative stress associated immune response inflammation after COVID-19. could be promising biomarker predicting may potential target therapeutic intervention

Language: Английский

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The emerging role of miRNAs in the pathogenesis of COVID-19: Protective effects of nutraceutical polyphenolic compounds against SARS-CoV-2 infection

International Journal of Medical Sciences, Journal Year: 2022, Volume and Issue: 19(8), P. 1340 - 1356

Published: Jan. 1, 2022

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can cause immunosuppression and cytokine storm, leading to lung damage death.The clinical efficacy of anti-SARS-CoV-2 drugs in preventing viral entry into host cells suppressing replication remains inadequate.MicroRNAs (miRNAs) are crucial the immune response pathogenesis coronaviruses, such as SARS-CoV-2.However, specific roles miRNAs life cycle SARS-CoV-2 remain unclear.miRNAs participate through at least four possible mechanisms: 1. cell miRNA expression interfering with entry, 2. SARS-CoV-2-derived RNA transcripts acting competitive endogenous RNAs (ceRNAs) that may attenuate expression, 3. modulating replication, 4. SARS-CoV-2-encoded silencing protein-coding genes.SARS-CoV-2-related be used diagnostic or prognostic biomarkers for predicting outcomes among patients infection.Furthermore, accumulating evidence suggests dietary polyphenolic compounds protect against by expression.These findings have major implications future diagnosis treatment COVID-19.

Language: Английский

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Interplay of host and viral factors in inflammatory pathway mediated cytokine storm during RNA virus infection

Current Research in Immunology, Journal Year: 2023, Volume and Issue: 4, P. 100062 - 100062

Published: Jan. 1, 2023

RNA viruses always have been a serious concern for human health by causing several outbreaks, often pandemics. The excessive mortality and deaths associated with the outbreaks caused these were due to induction of pro-inflammatory cytokines leading cytokine storm. Cytokines are important cell-to-cell communication maintain cell homeostasis. Disturbances this homeostasis can lead intricate chain reactions resulting in massive release cytokines. This could severe self-reinforcement feedback processes, which eventually cause systemic harm, multiple organ failure, or death. Multiple inflammation-associated pathways involved production its regulation. Different induce through interplay their viral factors host proteins miRNAs regulating pathways. review will discuss that play an role regulation storm possible therapeutic potential molecules treatment challenges clinical translation.

Language: Английский

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Advancing Ischemic Stroke Prognosis: Key Role of MiR-155 Non-Coding RNA

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 3947 - 3947

Published: April 22, 2025

Ischemic stroke (IS) is the leading cause of long-term disability and second death worldwide. It remains a significant clinical problem because only supportive therapies exist, such as thrombolytic agents surgical thrombectomy, which do not restore function. Understanding molecular pathogenesis IS, including dysfunction in oxidative homeostasis, apoptosis, neuroinflammation neuroprotection, crucial to developing therapies. Non-coding RNAs (ncRNAs) are master regulators, one ncRNA that stands out miR-155, pro-inflammatory micro-RNA elevated stroke. This review addresses biological mechanisms reported literature support using miR-155 biomarker therapeutic agent treat IS patients.

Language: Английский

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SARS-CoV-2-associated organs failure and inflammation: a focus on the role of cellular and viral microRNAs

Virology Journal, Journal Year: 2023, Volume and Issue: 20(1)

Published: Aug. 9, 2023

Abstract SARS-CoV-2 has been responsible for the recent pandemic all over world, which caused many complications. One of hallmarks infection is an induced immune dysregulation, in some cases resulting cytokine storm syndrome, acute respiratory distress syndrome and organs such as lungs, brain, heart that are affected during infection. Several physiological parameters altered a result storm. Among them, microRNAs (miRNAs) might reflect this poor condition since they play significant role cellular performance including inflammatory responses. Both host viral-encoded miRNAs crucial successful SARS-CoV-2. For instance, dysregulation modulate multiple genes expressed COVID-19 patients with comorbidities (e.g., type 2 diabetes, cerebrovascular disorders) could affect severity disease. Therefore, expression levels circulating be helpful to diagnose illness forecast whether patient develop severe state Moreover, number inhibit proteins, ACE2, TMPRSS2, spike, Nsp12, involved life cycle Accordingly, represent potential biomarkers therapeutic targets devastating viral In current study, we investigated modifications miRNA their influence on disease recovery, may employed therapy strategy minimize COVID-19-related disorders.

Language: Английский

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Long non-coding RNAs in biomarking COVID-19: a machine learning-based approach

Virology Journal, Journal Year: 2024, Volume and Issue: 21(1)

Published: June 7, 2024

Abstract Background The coronavirus pandemic that started in 2019 has caused the highest mortality and morbidity rates worldwide. Data on role of long non-coding RNAs (lncRNAs) disease (COVID-19) is scarce. We aimed to elucidate relationship three important lncRNAs inflammatory states, H19, taurine upregulated gene 1 (TUG1), colorectal neoplasia differentially expressed (CRNDE) with key factors inflammation fibrosis induction including signal transducer activator transcription3 (STAT3), alpha smooth muscle actin (α-SMA), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) COVID-19 patients moderate severe symptoms. Methods Peripheral blood mononuclear cells from 28 17 healthy controls were collected. real-time quantitative polymerase chain reaction (RT-qPCR) was performed evaluate expression lncRNAs. Western blotting analysis also determine levels STAT3 α-SMA proteins. Machine learning receiver operating characteristic (ROC) curve carried out distinguishing ability Results TUG1, CRNDE significantly overexpressed compared controls. Moreover, remarkedly increased at both transcript protein subjects correlated Three Likewise, IL-6 TNF-α considerably patients. ROC showed CRNDE-H19 panel proper distinguish individuals (area under (AUC) = 0.86). Conclusion overexpression observed this study may align significant manifestations COVID-19. Furthermore, their co-expression α-SMA, two critical implicated induction, underscores potential involvement exacerbating cardiovascular, pulmonary common symptoms complications associated combination H19 seems be an impressive host-based biomarker for screening diagnosis Research into can provide a robust platform find new viral infection-related mediators propose novel therapeutic strategies infections immune disorders.

Language: Английский

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A Comparative Analysis of MicroRNA Expression in Mild, Moderate, and Severe COVID-19: Insights from Urine, Serum, and Nasopharyngeal Samples

Biomolecules, Journal Year: 2023, Volume and Issue: 13(12), P. 1681 - 1681

Published: Nov. 21, 2023

COVID-19, caused by the SARS-CoV-2 virus, manifests with a wide range of clinical symptoms that vary from mild respiratory issues to severe distress. To effectively manage and predict outcomes disease, it is important understand molecular mechanisms underlying its severity. This study focuses on analyzing comparing expression patterns microRNAs (miRNAs) in serum, urine, nasopharyngeal samples patients mild, moderate, COVID-19. The aim identify potential associations disease progression discover suitable markers for diagnosis prognosis. Our findings indicate consistent upregulation miR-21, miR-146a, miR-155 In moderate cases, there were more significant changes miRNA compared cases. Specifically, miR-let-7 demonstrated upregulation, while miR-146b exhibited downregulation. most notable alterations profiles observed COVID-19 miR-223. Moreover, our analysis using Receiver-operating characteristic (ROC) curves miR-155, miR-let-7, miR-223 high sensitivity specificity, suggesting their as biomarkers distinguishing healthy individuals. Overall, this comparative revealed distinct expression. overlapping miRNAs suggest utility discriminating status.

Language: Английский

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Host factors of SARS-CoV-2 in infection, pathogenesis, and long-term effects

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: May 22, 2024

SARS-CoV-2 is the causative virus of devastating COVID-19 pandemic that results in an unparalleled global health and economic crisis. Despite unprecedented scientific efforts therapeutic interventions, fight against continues as rapid emergence different variants concern increasing challenge long COVID-19, raising a vast demand to understand pathomechanisms its long-term sequelae develop strategies beyond per se . Notably, addition itself, replication cycle clinical severity also governed by host factors. In this review, we therefore comprehensively overview pathogenesis from perspective factors host-virus interactions. We sequentially outline pathological implications molecular interactions between multi-organ multi-system summarize current agents targeting for treating these diseases. This knowledge would be key identification new pathophysiological aspects mechanisms, development actionable targets tackling sequelae.

Language: Английский

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