Whole-Genome Profiling of Endophytic Strain B.L.Ns.14 from Nigella sativa Reveals Potential for Agricultural Bioenhancement DOI Creative Commons

Dimitra Douka,

Tasos-Nektarios Spantidos,

Polina C. Tsalgatidou

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2604 - 2604

Published: Dec. 16, 2024

Endophytic microbes in medicinal plants often possess beneficial traits for plant health. This study focuses on the bacterial endophyte strain B.L.Ns.14, isolated from Nigella sativa leaves, which demonstrated multiple growth-promoting properties. In vitro tests showed that B.L.Ns.14 supports growth, colonization, and tolerance to abiotic stress. The also exhibited antifungal activity against phytopathogens such as Rhizoctonia solani, Colletotrichum acutatum, Verticillium dahliae, Fusarium oxysporum f. sp. radicis-lycopersici. Whole-genome analysis, supported by ANI dDDH values, identified Bacillus halotolerans. Genome mining revealed 128 active carbohydrate enzymes (Cazymes) related endophytism biocontrol functions, along with genes involved phosphate solubilization, siderophore IAA production, biofilm formation, motility. Furthermore, osmolyte metabolism, Na+/H+ antiporters, stress response proteins were identified. genome harbors 12 secondary metabolite biosynthetic gene clusters, including those surfactin, plipastatin mojavensin, rhizocticin A, bacilysin, known their antagonistic effects fungi. Additionally, promoted Arabidopsis thaliana growth under both normal saline conditions, enhanced Solanum lycopersicum via seed biopriming root irrigation. These findings suggest halotolerans holds potential a productivity agent, warranting further field testing.

Language: Английский

Enhancing dendrobine production in Dendrobium nobile through mono-culturing of endophytic fungi, Trichoderma longibrachiatum (MD33) in a temporary immersion bioreactor system DOI Creative Commons
Surendra Sarsaiya, Archana Jain,

Fuxing Shu

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 29, 2024

Dendrobine, a valuable alkaloid found in

Language: Английский

Citations

12
Community Structure and Diversity of Endophytic Fungi in Cultivated Polygala crotalarioides at Two Different Growth Stages Based on Culture-Independent and Culture-Based Methods DOI Creative Commons
Kaize Shen, Yu Xiong, Yanfang Liu

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(3), P. 195 - 195

Published: March 4, 2024

Polygala crotalarioides, a perennial herbaceous plant found in southwest China, has the potential to be used treatment of Alzheimer’s disease. Endophytic fungi that reside within medicinal herbs play an important ecological role their host plants and can serve as valuable source for identifying active components. However, little is known about diversity, structure endophytic P. crotalarioides. In this study, we investigated community diversity leaves, stems, roots crotalarioides at both 1- 2-year-growth stages using modern culture-independent method (high-throughput sequencing, HTS) culture-based methods. Using HTS, our results revealed richness varied depending on organs growth stages. Specifically, stems leaves exhibited significantly higher compared roots. Additionally, highest was observed 2-year-old plants. At genus level, Fusarium, Colletotrichum, Phoma were most abundant 1-year-old samples, while Cercospora, Apiotrichum, Fusarium prevalent samples. A total 55 fungal strains belonging two phyla 24 genera isolated from 150 tissue segments The anti-acetylcholinesterase activity these isolates evaluated vitro five them, Phialophora mustea PCAM010, Diaporthe nobilis PCBM027, oxysporum LP41, F. SR60, herbarum SM81, showed strong (>50% inhibition rate). These findings will theoretical basis practical guide comprehending structural composition, biological bioactivity

Language: Английский

Citations

5
Biocontrol efficacy and mechanism of endophytic Bacillus velezensis HE-23 from Aucklandia lappa roots against the gray mold of postharvest tomato DOI
Jian Wei, Yu Chen, Shiyu Liu

et al.

Postharvest Biology and Technology, Journal Year: 2024, Volume and Issue: 217, P. 113104 - 113104

Published: July 18, 2024

Language: Английский

Citations

5
Bioprospecting Endophytic Fungi from Fusarium Genus as Sources of Bioactive Metabolites DOI

K K Purushothaman,

Esaimozhi Sivasankar,

V. G. Sowmeya

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Two undescribed benzofuran derivatives from chaetomium elatum DOI

Changhong Yan,

Feng Ju,

Yuhui Chen

et al.

Natural Product Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 5

Published: Feb. 21, 2025

Two undescribed benzofuran derivatives (1-2) were isolated from Chaetomium elatum using silica gel, Sephadex LH-20 column chromatography, and preparative high-performance liquid chromatography (HPLC). The structures of compound 1-2 characterised by HRESIMS, NMR, HMBC, NOESY spectral analyses, ECD calculations. Compounds demonstrated the ability to mitigate inflammatory response triggered lipopolysaccharide (LPS) in RAW264.7 macrophages, as evidenced RT-PCR assay results showing suppression key factors at concentration 30 μM. These findings suggest that possess promising anti-inflammatory properties.

Language: Английский

Citations

0
Whole-Genome Profiling of Endophytic Strain B.L.Ns.14 from Nigella sativa Reveals Potential for Agricultural Bioenhancement DOI Creative Commons

Dimitra Douka,

Tasos-Nektarios Spantidos,

Polina C. Tsalgatidou

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2604 - 2604

Published: Dec. 16, 2024

Endophytic microbes in medicinal plants often possess beneficial traits for plant health. This study focuses on the bacterial endophyte strain B.L.Ns.14, isolated from Nigella sativa leaves, which demonstrated multiple growth-promoting properties. In vitro tests showed that B.L.Ns.14 supports growth, colonization, and tolerance to abiotic stress. The also exhibited antifungal activity against phytopathogens such as Rhizoctonia solani, Colletotrichum acutatum, Verticillium dahliae, Fusarium oxysporum f. sp. radicis-lycopersici. Whole-genome analysis, supported by ANI dDDH values, identified Bacillus halotolerans. Genome mining revealed 128 active carbohydrate enzymes (Cazymes) related endophytism biocontrol functions, along with genes involved phosphate solubilization, siderophore IAA production, biofilm formation, motility. Furthermore, osmolyte metabolism, Na+/H+ antiporters, stress response proteins were identified. genome harbors 12 secondary metabolite biosynthetic gene clusters, including those surfactin, plipastatin mojavensin, rhizocticin A, bacilysin, known their antagonistic effects fungi. Additionally, promoted Arabidopsis thaliana growth under both normal saline conditions, enhanced Solanum lycopersicum via seed biopriming root irrigation. These findings suggest halotolerans holds potential a productivity agent, warranting further field testing.

Language: Английский

Citations

0