Fe₃O₄-Based Nanospheres with High Photothermal Conversion Efficiency for Dual-Effect and Mild Biofilm Eradication against Periodontitis

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 26, 2025

Periodontitis, a chronic inflammatory oral disease resulting from plaque biofilms, affects about 743 million individuals worldwide. However, the efficacy of current treatments is hampered by challenges in delivering antibiotics to recalcitrant biofilms and bacterial resistance, thereby impeding successful treatment infectious diseases. To address issues, an antibacterial photothermal material was designed, comprising spherical structure zinc oxide (ZnO) wrapped with triiron tetraoxide (Fe3O4). The outer layer adsorbed epsilon-polylysine (EPL) electrostatic action, ultimately leading fabrication Fe3O4/ZnO/EPL nanoparticles (FZE NPs). Fe3O4 core endowed efficient properties, facilitating dispersion dense which dramatically promoted adsorption penetration ZnO EPL into effectively kill bacteria vitro enhanced sterilization ability. Additionally, upon dissolution aqueous media, acts as positively charged antimicrobial peptide that adsorbs onto surface negatively membranes, modulating responses. In order ascertain FZE NPs, investigation conducted their effects against periodontitis-associated pathogen Porphyromonas gingivalis (P. gingivalis) vitro. Furthermore, antiperiodontitis potential NPs evaluated Sprague-Dawley (SD) rats ligamentous periodontitis. addition, toxicity evaluations indicated had acceptable biosafety profile vivo. summary, nanospheres NPs) represent promising therapeutic strategy for

Language: Английский

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Controlling Alveolar Bone Loss by Hydrogel‐Based Mitigation of Oral Dysbiosis and Bacteria‐Triggered Proinflammatory Immune Response

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 12, 2024

Abstract Periodontitis is a chronic infection where abnormal host‐microbiota interactions alter the oral microbiome, trigger proinflammatory immune response, and cause inflammatory alveolar bone loss. While antibiotics are occasionally necessary for treating periodontitis, their use must be carefully managed to prevent development of drug resistance dysbiosis. Therefore, it's crucial develop new treatment strategies periodontitis that reduce antibiotic dependence while effectively controlling inflammation triggered by bacteria. In this study, hydrogel engineered grafting cationic polyamidoamine dendrimers (PAMAM‐G3) onto oxidized carboxymethyl cellulose (OCMC) backbone, resulting in an injectable (OCMC‐PAMAM‐G3, O‐P). This can capture anionic microbial‐associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS) cell‐free DNA (cfDNA). These findings reveal using O‐P application circumvents disruption mucosa microbiome caused traditional antibiotics. Additionally, mitigate loss ligature‐induced mouse model alleviating LPS/cfDNA‐TLR4/9 pathway. Moreover, topical administration has no significant adverse effects on improving local subgingival microbiome. The study highlights strategy targeting MAMPs avoiding antibiotics, it bacteria‐triggered response potentially preserve

Language: Английский

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Alleviating effect on periodontal inflammation through anti-inflammatory and osteogenic differentiation induction of KGC19b in vitro and in vivo experimental model

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Background Panax ginseng C.A. Meyer of the Araliaceae family have various biological activities and pharmacological actions such as anticancer, immunomodulation, anti-inflammatory. In many studies on ginseng, ginseng fruit are still new, effect potential periodontitis in HPDL cells been investigated in vitro vivo. Methods The effects KGC19b a Porphyromonas gingivalis lipopolysaccharide (Pg-LPS)-induced model were assessed using cultured an SD-rats model. stimulated with Pg-LPS for 12 h grouped into control, Pg-LPS, + KGC19b. Cell viability, pro-inflammatory markers, reactive oxygen species (ROS), osteogenesis-related markers evaluated. rat model, was induced ligature Pg-LPS. administered orally 6 days. Rats divided groups (n = 6): ligature/Pg-LPS, ligature/Pg-LPS (each dose group). Effects through micro-CT analysis H&E staining. Results significantly reduced inflammatory cytokines (IL-6, IL-1β, TNF-α) mediators It also restored osteocalcin (ocn) alkaline phosphatase (alp) levels, enhanced antioxidant enzyme expression (SOD, CAT), reducing (ROS). alleviated periodontal inflammation prevented bone mass loss. Conclusion this study, shows possibility improving treating anti-inflammatory, osteogenesis, antibacterial effects.

Language: Английский

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Biodegradable Piezoelectric Janus Membrane Enabling Dual Antibacterial and Osteogenic Functions for Periodontitis Therapy

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Guided tissue regeneration (GTR) using barrier membranes is a common clinical approach for treating periodontitis-induced alveolar bone loss. However, conventional GTR lack antibacterial and osteoinductive properties, limiting their effectiveness. Piezoelectric materials, which generate electrical outputs under chewing forces, offer bone-regenerative potential due to oppositely charged surfaces. Inspired by this, piezoelectric Janus membrane was developed dual-function therapy. Biodegradable poly(l-lactide) (PLLA) PLLA/gelatin were electrospun, annealed, polarized create the A-P(+)/PG(-) membrane. Notably, in this membrane, outer surface of PLLA side (A-P(+)) carries positive charges positioned toward gingival kill bacteria via charge interactions; inner PG (PG(-)) holds negative faces defect, promoting growth through immunomodulation enhanced mineralization. In mouse model periodontitis, demonstrated dual functionality, effectively reducing inflammation, inhibiting resorption. The mineral density reached 1637 ± 37 mg/cm3 at 8 weeks after surgery, superior commercial collagen lacking properties. Overall, study introduces an innovative approach, leveraging biodegradable construct versatile with osteogenic activity periodontitis treatment.

Language: Английский

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Oral Health and Caries Prevention: How Tongue Hygiene Helps Maintain Balance of Microbiota and Overall Health in Pediatric Patients

Children, Journal Year: 2024, Volume and Issue: 11(7), P. 816 - 816

Published: July 3, 2024

Background/Objectives: The tongue harbors about two-thirds of the microorganisms present in mouth; stable bacterial population consists mainly aerobic and facultative anaerobic streptococci. These colonies, found more frequently on than outside hard part dental enamel children younger 18 months, suggest that is a potential reservoir. aim this review to examine scientific literature clarify whether mechanical removal biofilm can have positive effect caries prevention, with reduction colony-forming unit (CFU) salivary streptococcus whole-mouth plaque index (FMPS). Methods: An open search was conducted by using PubMed (MEDLINE), Cochrane Library Google Scholar. most studied age range 9 12 years, groups no minimum number two teeth were reconstructed, decayed and/or missing (DMFS/dmfs > 2) who experienced different hygiene methods for first time. Results: Four randomized trials met criteria included review. Conclusions: results obtained specific protocols, combined healthy diet lifestyle, could be considered gold standard enable effective primary prevention improve health pediatric patients. This improves understanding impact controlling bacteria responsible onset carious disease its systemic correlates; however, further research data needed confirm findings research.

Language: Английский

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Microbial Dynamics in Periodontal Regeneration: Understanding Microbiome Shifts and the Role of Antifouling and Bactericidal Materials: A Narrative Review

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(11), P. 12196 - 12213

Published: Oct. 30, 2024

Periodontal regeneration is a multifaceted therapeutic approach to restore the tooth-supporting structures lost due periodontal diseases. This manuscript explores intricate interactions between regenerative therapies and oral microbiome, emphasizing critical role of microbial balance in achieving long-term success. While guided tissue (GTR), bone grafting, soft grafting offer promising outcomes terms regeneration, these procedures can inadvertently alter ecosystem, potentially leading dysbiosis or pathogenic recolonization. Different materials, including autografts, allografts, xenografts, alloplasts, influence shifts, with variations healing timeline stabilization. Biologics antimicrobials, such as enamel matrix derivatives (EMD) sub-antimicrobial dose doxycycline (SDD), play key promoting homeostasis by supporting repair reducing bacteria. Emerging strategies, enzyme-based antifouling aim disrupt biofilm formation enhance effectiveness treatments. Understanding dynamics essential for optimizing improving patient outcomes. The future therapy lies development advanced materials strategies that not only tissues but also stabilize ultimately health.

Language: Английский

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Advancements in Autophagy Modulation for the Management of Oral Disease: A Focus on Drug Targets and Therapeutics

Biomedicines, Journal Year: 2024, Volume and Issue: 12(11), P. 2645 - 2645

Published: Nov. 19, 2024

Autophagy is an intrinsic breakdown system that recycles organelles and macromolecules, which influences metabolic pathways, differentiation, thereby cell survival. Oral health essential component of integrated well-being, it critical for developing therapeutic interventions to understand the molecular mechanisms underlying maintenance oral homeostasis. However, because complex dynamic relationship between autophagy health, associated treatment modalities have not yet been well elucidated. Determining how affects at level may enhance understanding prevention targeted diseases. At level, hard soft tissues develop interactions epithelial mesenchymal cells. Aging contributes progression various disorders including periodontitis, cancer, periapical lesions during aging. levels decrease with age, thus indicating a possible association In this review, we critically review aspects their significance in context diseases lesions, periodontal conditions, candidiasis. A better its can guide us new preventative strategies management

Language: Английский

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The Effect of Selenase on Inflammatory and Cytoprotective Markers in Experimental Chronic Generalized Periodontitis

Biomedical & Pharmacology Journal, Journal Year: 2024, Volume and Issue: 17(3), P. 1911 - 1919

Published: Sept. 30, 2024

Epidemiological studies in recent decades have revealed a significant increase the number of patients with periodontal diseases leading to tooth loss. Modern realities require improvement drug treatment periodontitis. The antioxidant Selenase, selenium derivative, is an interesting strategy for study was carried out aim evaluate healing effectiveness Selenase rats chronic generalized periodontitis (CGP) by its effect on markers inflammation and cytoprotection. Experimental CGP modulated Wistar calcium-deficient diet inclusion prooxidant. (50 mcg/kg) Mexidol (ethylmethylhydroxypyridine succinate, 250 mg/kg) were administered intragastrically 30 days. Levels IL-1β, HIF-1α, HSP70, TNF-α determined blood after using enzyme immunoassay method. characterized development hyperemia, swelling, bleeding gums; mobility teeth; gingival pockets up 8 mm against background increased inflammatory (IL-1β, TNF-α), molecular cytoprotection (HIF-1α, HSP70) blood, indicating homeostatic response periodontium subsequent hypoxia. Administration produced pronounced effects: reduction depth 42.55 %, cessation bleeding, disappearance swelling decrease markers: IL-1β – 44.6 65.9 % (p < 0.05). HIF-1α 36.8 HSP70 71.1 compared those control group, which not given results obtained suggest influence HSP70-dependent mechanisms endogenous found that use experimental more effective than Mexidol.

Language: Английский

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Classification and bibliometric analysis of hydrogels in periodontitis treatment: Trends, mechanisms, advantages, and future research directions

Dental Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

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