Evaluation of mesoporous silica particles as a support for lipase immobilization in biodiesel production: Enhanced ethyl ester synthesis from algal oil

Results in Engineering, Journal Year: 2024, Volume and Issue: 22, P. 102138 - 102138

Published: April 16, 2024

The utilization of novel materials in the biofuel production domain has been regarded as a significant advancement catalyst development. Incorporating lipases biodiesel manufacturing is often viewed cost-restrictive phase since expenses associated with recovering these catalysts can render market expectations unfeasible. Within this investigation, mesoporous silica (MPS) particles were assessed supporting medium for immobilizing commercial lipase, subsequent to their ethyl ester synthesis. Initially, was achieved through co-precipitation technique at consistent pH and subsequently chosen an immobilization support enzyme. characterization biocatalyst produced performed using various analytical methods including X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), BET analysis. Novozym 435 lipase then immobilized within matrices, followed by transesterification process algal oil derived from Nannochloropsis. resulting samples demonstrated compliance standards, exhibiting conversion rate exceeding 94.8%. Additionally, properties such density (0.93 g.cm-3) viscosity (5.01 mm2.s-1) satisfied specifications mandated ASTM application. Experimental planning techniques showed that increasing loading temperatures between 38 48 °C resulted higher rates esters. This confirms effectiveness enzymatic generating primary fatty acid esters, even residual lipid feedstocks.

Language: Английский

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Algae’s potential as a bio-mass source for bio-fuel production: MLR vs. ANN models analyses

Fuel, Journal Year: 2025, Volume and Issue: 395, P. 134853 - 134853

Published: March 28, 2025

Language: Английский

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Immunomodulatory Compounds from the Sea: From the Origins to a Modern Marine Pharmacopoeia

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(7), P. 304 - 304

Published: June 28, 2024

From sea shores to the abysses of deep ocean, marine ecosystems have provided humanity with valuable medicinal resources. The use organisms is discussed in ancient pharmacopoeias different times and geographic regions still deeply rooted traditional medicine. Thanks present-day, large-scale bioprospecting rigorous screening for bioactive metabolites, ocean coming back as an untapped resource natural compounds therapeutic potential. This renewed interest drugs propelled by a burgeoning research field investigating molecular mechanisms which newly identified intervene pathophysiology human diseases. Of great clinical relevance are molecules endowed anti-inflammatory immunomodulatory properties emerging applications management chronic inflammatory disorders, autoimmune diseases, cancer. Here, we review historical development pharmacology Eastern Western worlds describe status drug discovery. Finally, discuss importance conducting sustainable exploitation resources through biotechnology.

Language: Английский

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Turning the industrially relevant marine alga Nannochloropsis red: one move for multifaceted benefits

New Phytologist, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Summary Nannochloropsis oceanica is an industrially relevant marine microalga rich in eicosapentaenoic acid (EPA, a valuable ω‐3 polyunsaturated fatty acid), yet the algal production potential remains to be unlocked. Here we engineered N. synthesize high‐value carotenoid astaxanthin independent of high‐light (HL) induction for achieving multifaceted benefits. By screening β‐carotenoid ketolases and hydroxylases various origins, strategically manipulating compartmentalization, fusion patterns, linkers enzyme pair, remarkable 133‐fold increase content was achieved . Iterative metabolic engineering efforts led further increases synthesis up 7.3 mg g −1 , highest reported microalgae under nonstress conditions. Astaxanthin found photosystem components allowed alga HL resistance augmented EPA production. Besides, co‐production by through fed‐batch cultivation approach. Our findings unveil untapped as robust, light‐driven chassis constitutive provide feasible strategies concurrent multiple biochemicals from CO 2 thereby paving way sustainable biotechnological applications this alga.

Language: Английский

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Engineering Nannochloropsis oceanica for concurrent production of canthaxanthin and eicosapentaenoic acid

Bioresource Technology, Journal Year: 2024, Volume and Issue: unknown, P. 131525 - 131525

Published: Sept. 1, 2024

Language: Английский

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Improving Undernutrition with Microalgae

Nutrients, Journal Year: 2024, Volume and Issue: 16(18), P. 3223 - 3223

Published: Sept. 23, 2024

Undernutrition is an important global health problem, especially in children and older adults. Both reversal of maternal child undernutrition heathy ageing have become United Nations-supported initiatives, leading to increased attention nutritional interventions targeting undernutrition. One feasible option microalgae, the precursor all terrestrial plants. Most commercially farmed microalgae are photosynthetic single-celled organisms producing organic carbon compounds oxygen. This review will discuss commercial opportunities grow microalgae. Microalgae produce lipids (including omega-3 fatty acids), proteins, carbohydrates, pigments micronutrients so can provide a suitable underutilised alternative for addressing The benefits nutrients derived from been identified, thus they candidates issues globally. potential microalgae-derived be converted into food products. advantages cultivation include that it does not need arable land or pesticides. Additionally, most species still unexplored, presenting options further development. Further, usefulness other purposes such as bioremediation biofuels increase knowledge these microorganisms, allowing development more efficient production interventions.

Language: Английский

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Transcriptomic Signature of Lipid Production in Australian Aurantiochytrium sp. TC20

Marine Biotechnology, Journal Year: 2025, Volume and Issue: 27(1)

Published: Feb. 1, 2025

Abstract Aurantiochytrium not only excels in producing long-chain polyunsaturated fatty acids such as docosahexaenoic acid for humans, but it is also a source of essential with minimal impacts on wild fisheries and vital the transfer atmospheric carbon to oceanic sinks cycles. This study aims unveil systems biology lipid production Australian sp. TC20 by comparing transcriptomic profiles under optimal growth conditions increased from early (Day 1) late exponential phase 3). Particular attention was paid 227 manually annotated genes involved metabolism, FAS (fatty synthetase) subunits ( PUFA ) synthase. PCA analysis showed that differentially expressed genes, related efficiently discriminated Day 3 samples 1, highlighting key robustness developed lipid-biosynthesis signature. Highly significant (pFDR < 0.01) upregulation synthase subunit B PFAB synthesis, droplet protein TLDP TAG-synthesis, phosphoglycerate mutase PGAM-2 glycolysis gluconeogenesis were observed. KEGG enrichment highlighted biosynthesis unsaturated metabolism pathways 0.01). provides comprehensive overview transcriptional landscape process production.

Language: Английский

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Novel Approach to Enhancing Brain DHA Uptake: The Role of Nannochloropsis Microalgae Extract

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Docosahexaenoic acid (DHA) plays a pivotal role in sustaining the normal function of human brain and preventing metabolic neurodegenerative disorders. Despite its significance, bioavailability DHA from current supplements is suboptimal due to limited uptake capacity adult brain. Recent studies have highlighted Mfsd2a transporter facilitating when esterified lyso-phosphatidylcholine (LPC). Lyso-diacylglyceryltrimethylhomoserine (LDGTS), betaine lipid that resembles LPC structure abundant Nannochloropsis microalgaeoil, presents promising alternative for enhancing uptake. This study aimed examine whether LDGTS-containing microalgae oil can increase level by We established 8 groups mice fed with different materials comparison addition their regular diet. After 15 days feeding, tissue organ samples were collected extraction fatty analysis. The mixture triacylglycerol (TAG)-DHA showed significant compared control. effect was not observed administered TAG-DHA alone, or krill oil. facilitation incorporation accompanied notable enhancement pathways related cell growth retinol metabolism brain, while associated cellular apoptosis inflammation downregulated. Thus, inclusion LDGTS-rich supplementation may offer novel effective strategy improving

Language: Английский

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Marine Phytoplankton Bioactive Lipids and Their Perspectives in Clinical Inflammation

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(2), P. 86 - 86

Published: Feb. 17, 2025

Marine phytoplankton is an emerging source of immunomodulatory bioactive lipids (BLs). Under physiological growth conditions and upon stress challenges, several eukaryotic microalgal species accumulate lipid metabolites that resemble the precursors animal mediators inflammation: eicosanoids prostaglandins. Therefore, marine could serve as a biotechnological platform to produce functional BLs with therapeutic applications in management chronic inflammatory diseases other clinical conditions. However, be commercially competitive, lipidic precursor yields should enhanced. Beside tailoring cultivation native producers, genetic engineering feasible strategy accrue production introduce heterologous biosynthetic pathways hosts. Here, we present state-of-the-art research on from discuss synthetic biology approaches boost their light-driven biosynthesis.

Language: Английский

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Bioremediation of Synthetic Wastewater with Contaminants of Emerging Concern by Nannochloropsis sp. and Lipid Production: A Circular Approach

Bioengineering, Journal Year: 2025, Volume and Issue: 12(3), P. 246 - 246

Published: Feb. 28, 2025

Contaminants of emerging concern (CECs) pose a potential risk to human and environmental health. Microalgae bioremediation is promising approach for transforming or removing contaminants from the environment, while contributing circular economy. In this study, Nannochloropsis sp. was effectively used simultaneous removal six CECs: paracetamol, ibuprofen, imidacloprid, methylparaben bisphenol A at 10 µg mL−1 triclosan 0.5 synthetic wastewater, which were able survive under such concentrations, higher than those commonly found in environment (up 2.82 methylparaben). High efficiencies reached (100%) (93 ± 2%), paracetamol 30 1%, 64 2% 49 5% removed, respectively. Subsequently, lipids extracted, FAME profile characterised using GS-MS. The main fatty acids identified after hexadecadienoic acid isomers (C16:2), palmitic (C16), linoleic (C18:2) γ-linolenic (C18:3). absence oleic stearic noticed, suggesting an alteration lipidic due contaminant exposure. By exploring quantification future work, applications extracted can be explored, further demonstrating feasibility process.

Language: Английский

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