Olaris Global Panel (OGP): A Highly Accurate and Reproducible Triple Quadrupole Mass Spectrometry-Based Metabolomics Method for Clinical Biomarker Discovery DOI Creative Commons
Masoumeh Dorrani,

Jifang Zhao,

Nihel Bekhti

et al.

Metabolites, Journal Year: 2024, Volume and Issue: 14(5), P. 280 - 280

Published: May 11, 2024

Mass spectrometry (MS)-based clinical metabolomics is very promising for the discovery of new biomarkers and diagnostics. However, poor data accuracy reproducibility limit its true potential, especially when performing analysis across multiple sample sets. While high-resolution mass has gained considerable popularity metabolomics, triple quadrupole (QqQ) instruments offer several benefits measurement known metabolites in samples. These include high sensitivity a wide dynamic range. Here, we present Olaris Global Panel (OGP), HILIC LC-QqQ MS method comprehensive ~250 from all major metabolic pathways For development this method, columns mobile phase conditions were compared, robustness leading LC assessed, acquisition settings optimized optimal quality. Next, effect U-13C metabolite yeast extract spike-ins was assessed based on precision. The use these U-13C-metabolites as internal standards improved goodness fit to linear calibration curve r2 < 0.75 raw >0.90 most entire concentration range urine Median within-batch CVs ratios consistently lower than 7% less 10% batches that acquired over six-month period. Finally, OGP ability identify biomarkers, confirmed using large set.

Language: Английский

Challenges and recent advances in quantitative mass spectrometry‐based metabolomics DOI Open Access

Nathan Ghafari,

Lekha Sleno

Analytical Science Advances, Journal Year: 2024, Volume and Issue: 5(5-6)

Published: June 1, 2024

Abstract The field of metabolomics has gained tremendous interest in recent years. Whether the goal is to discover biomarkers related certain pathologies or better understand impact a drug contaminant, numerous studies have demonstrated how crucial it variations metabolism. Detailed knowledge metabolic variabilities can lead more effective treatments, as well faster less invasive diagnostics. Exploratory approaches are often employed metabolomics, using relative quantitation look at perturbations between groups samples. Most been based on metabolite profiling quantitation, with very few an approach for absolute quantitation. Using accurate facilitates comparison different studies, enabling longitudinal studies. In this review, we discuss most widely used techniques quantitative mass spectrometry (MS). Various aspects will be addressed, such use external and/or internal standards, derivatization techniques, vivo isotopic labelling, MS imaging. principles, associated limitations and challenges, described each approach.

Language: Английский

Citations

7
Advancing Clinical Liquid Chromatography-Mass Spectrometry Analysis: Rational Design and Optimization of Derivatization Techniques for Enhanced Sensitivity and Throughput DOI Creative Commons
Hua‐Ming Xiao, А. З. Темердашев, Na An

et al.

Journal of Chromatography Open, Journal Year: 2025, Volume and Issue: unknown, P. 100215 - 100215

Published: March 1, 2025

Language: Английский

Citations

0
Urinary metabolomics in rats following gamma-hydroxybutyric acid administration: Identification of 4-guanidinobutyric acid as a novel GHB biomarker metabolite DOI Creative Commons
Suji Kim, Sang-Hoon Song, Mingyu Kim

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: 213, P. 113793 - 113793

Published: April 28, 2025

Language: Английский

Citations

0
Analysis of variable metabolites in preterm infants with bronchopulmonary dysplasia: a systematic review and meta-analysis DOI Creative Commons
Yanping Guo, Ying Liu, Ruolin Zhang

et al.

˜The œItalian Journal of Pediatrics/Italian journal of pediatrics, Journal Year: 2024, Volume and Issue: 50(1)

Published: Nov. 14, 2024

Abstract Numerous studies have attempted to identify potential biomarkers for early detection of bronchopulmonary dysplasia (BPD) in preterm infants using metabolomics techniques. However, the presence consistent evidence remains elusive. Our study aimed conduct a systematic review and meta-analysis differences small-molecule metabolites between BPD non-BPD infants. Through meticulous screening numerous samples, we identified promising candidates, providing valuable insights future research. We searched PubMed, Cochrane Library, Embase, Web Science, China National Knowledge Internet, Wan-fang database, Chinese Science Technique Journal Database Biomedical Literature from inception until January 16, 2024. Studies were comprehensively reviewed against inclusion criteria. included case-control adhered Preferred Reporting Items Systematic Reviews Meta-Analysis guidelines. Study quality was assessed with Newcastle-Ottawa scale. compared changes metabolite levels A conducted on targeted research data based strategy standardized mean (MD) 95% confidence intervals (CI).Fifteen (1357 participants) included. These clinical-based clarified 110 differential The revealed higher glutamate concentration group (MD = 1, CI 0.59 1.41, p < 0.00001). Amino acids as key distinguishing without BPD, potentially serving predictor this population.

Language: Английский

Citations

1
Olaris Global Panel (OGP): A Highly Accurate and Reproducible Triple Quadrupole Mass Spectrometry-Based Metabolomics Method for Clinical Biomarker Discovery DOI Creative Commons
Masoumeh Dorrani,

Jifang Zhao,

Nihel Bekhti

et al.

Metabolites, Journal Year: 2024, Volume and Issue: 14(5), P. 280 - 280

Published: May 11, 2024

Mass spectrometry (MS)-based clinical metabolomics is very promising for the discovery of new biomarkers and diagnostics. However, poor data accuracy reproducibility limit its true potential, especially when performing analysis across multiple sample sets. While high-resolution mass has gained considerable popularity metabolomics, triple quadrupole (QqQ) instruments offer several benefits measurement known metabolites in samples. These include high sensitivity a wide dynamic range. Here, we present Olaris Global Panel (OGP), HILIC LC-QqQ MS method comprehensive ~250 from all major metabolic pathways For development this method, columns mobile phase conditions were compared, robustness leading LC assessed, acquisition settings optimized optimal quality. Next, effect U-13C metabolite yeast extract spike-ins was assessed based on precision. The use these U-13C-metabolites as internal standards improved goodness fit to linear calibration curve r2 < 0.75 raw >0.90 most entire concentration range urine Median within-batch CVs ratios consistently lower than 7% less 10% batches that acquired over six-month period. Finally, OGP ability identify biomarkers, confirmed using large set.

Language: Английский

Citations

0