Scaffold-Free Bone-Like 3d Structure Established Through Osteogenic Differentiation from Human Gingiva-Derived Stem Cells DOI

Masaaki Toyoda,

Takao Fukuda,

Ryota Fujimoto

et al.

Published: Jan. 1, 2023

Over the past decade, stem cell therapy has been rigorously examined for clinical applications, though its widespread adoption remains limited despite some trials demonstrating promising outcomes. This therapeutic approach is particularly anticipated to hold significant potential in regenerative medicine conditions including traumatic bone defects, iatrogenic oncological resections, congenital abnormalities, and severe periodontitis oral region.Historically, remediation of extensive defects was believed necessitate integration biomaterials with cell-derived structures. Recently, however, Bio-3D printer "Regenova®" emerged as a cutting-edge three-dimensional culture system, employing scaffold-free bio-assembly techniques. robotic system plays pilling cellular spheroids attached stainless steel needle array, reminiscent closely packed BBQ-style skewers, according pre-defined configurations.Interestingly, gingiva-derived mesenchymal cells (GMSCs) manifest attributes superior both marrow-derived adipose cells, advantages such simplified isolation processes enhanced proliferation rates.Our research led creation validation spheroids, approximately 600 μm diameter, derived from human GMSCs (hGMSCs). These have proven retain their inherent capabilities osteogenic differentiation potentials. Markedly, our team achieved pioneering feat crafting scaffold-free, bone-mimetic luminal structure via assembly composed hGMSCs. breakthrough holds considerable promise advancing applications within domain medicine, defect conditions.

Language: Английский

3D bioprinting advanced biomaterials for craniofacial and dental tissue engineering – A review DOI Creative Commons
Hailong Xu,

Yuran Ge,

Yidan Zhang

et al.

Materials & Design, Journal Year: 2024, Volume and Issue: 241, P. 112886 - 112886

Published: March 28, 2024

The rising incidence of defects in oral and maxillofacial tissues, linked to factors such as trauma, tumors, periodontal disease, aging, poses significant challenges. Current treatments, involving autografts, allografts, synthetic graft materials, face obstacles like secondary inflammation, inadequate biocompatibility. Tissue engineering, integrating cell biology material science since the 1990s, relies heavily on biomaterial scaffolds promote adhesion, proliferation, differentiation. Traditional scaffold fabrication, including 3D printing, methods lack precision, hindering effective tissue repair by controlling distribution extracellular matrix. Biomedical engineering advancements have introduced bioprinting an innovative solution, overcoming constraints conventional scaffolds. technology enables rapid precise reconstruction damaged tissues with loaded cells, mimicking vivo environments. This paper explores key technologies inkjet-based, extrusion-based, fused deposition modeling, laser-assisted, VAT photopolymerization, freeform reversible embedding suspended hydrogels, sacrificial template printing. selection materials suitable mechanical biological properties is crucial, considering distinct requirements each technique. review provides a comprehensive survey research progress printing applications craniofacial dental serving valuable reference for future medical research.

Language: Английский

Citations

24
Emerging advanced approaches for diagnosis and inhibition of liver fibrogenesis DOI Creative Commons

Tamer A. Addissouky,

Majeed M. A. Ali, Ibrahim El Tantawy El Sayed

et al.

The Egyptian Journal of Internal Medicine, Journal Year: 2024, Volume and Issue: 36(1)

Published: Feb. 12, 2024

Abstract Background Liver fibrosis results from chronic liver injury and is characterized by excessive deposition of extracellular matrix proteins including collagen. It can progress to cirrhosis failure. Main body the abstract Multiple cellular signaling pathways drive hepatic stellate cell activation fibrogenesis. Advances in biomarkers, imaging modalities, omics platforms enable noninvasive diagnosis staging fibrosis. Emerging antifibrotic approaches include medications like pirfenidone, obeticholic acid, monoclonal antibodies targeting pro-fibrotic mediators. Cell therapies using mesenchymal stem cells demonstrate potential through paracrine immunosuppression. Tissue-engineered grafts biomaterial carriers for localized drug delivery are promising technologies. Microfluidic liver-on-a-chip with patient-derived provide unprecedented models study human test candidates. Short conclusion Significant has elucidated mechanisms underlying fibrogenesis uncovered novel therapeutic targets. Ongoing challenges translating preclinical findings, improving efficacy, enabling personalized precision medicine approaches. Further research into combinatorial therapies, tissue engineering technologies will advance treatment all causes.

Language: Английский

Citations

9
Recent advances in Organ-on-a-Chip models: How precision engineering integrates cutting edge technologies in fabrication and characterization DOI
Sorour Sadeghzade, Mohammad Hosseini Hooshiar, Hossein Akbari

et al.

Applied Materials Today, Journal Year: 2024, Volume and Issue: 38, P. 102231 - 102231

Published: May 14, 2024

Language: Английский

Citations

9
Three-Dimensional Printing/Bioprinting and Cellular Therapies for Regenerative Medicine: Current Advances DOI Creative Commons
Ana Catarina Sousa, Rui Alvites, Bruna Lopes

et al.

Journal of Functional Biomaterials, Journal Year: 2025, Volume and Issue: 16(1), P. 28 - 28

Published: Jan. 16, 2025

The application of three-dimensional (3D) printing/bioprinting technologies and cell therapies has garnered significant attention due to their potential in the field regenerative medicine. This paper aims provide a comprehensive overview 3D technology therapies, highlighting results diverse medical applications, while also discussing capabilities limitations combined use. synergistic combination printing cellular been recognised as promising innovative approach, it is expected that these will progressively assume crucial role treatment various diseases conditions foreseeable future. review concludes with forward-looking perspective on future impact technologies, revolutionize medicine through enhanced tissue repair organ replacement strategies.

Language: Английский

Citations

1
Innovations in 3D printed individualized bone prosthesis materials: revolutionizing orthopedic surgery: a review DOI Creative Commons

Zhigang Qu,

Jiaji Yue, Ning Song

et al.

International Journal of Surgery, Journal Year: 2024, Volume and Issue: 110(10), P. 6748 - 6762

Published: June 21, 2024

The advent of personalized bone prosthesis materials and their integration into orthopedic surgery has made a profound impact, primarily as result the incorporation three-dimensional (3D) printing technology. By leveraging digital models additive manufacturing techniques, 3D enables creation customized, high-precision implants tailored to address complex anatomical variabilities challenging defects. In this review, we highlight significant progress in utilizing printed prostheses across wide range procedures, including pelvis, hip, knee, foot, ankle, spine surgeries, tumor resections. preoperative planning, surgical navigation, postoperative rehabilitation not only enhances treatment outcomes but also reduces risks, accelerates recovery, optimizes cost-effectiveness. Emphasizing potential for care improved patient outcomes, review underscores pivotal role advancing practice towards precision, efficiency, patient-centric solutions. evolving landscape holds promise revolutionizing approaches, enhancing ultimately improving quality patients.

Language: Английский

Citations

6
Applications of Light-Based 3D Bioprinting and Photoactive Biomaterials for Tissue Engineering DOI Open Access
Xueqin Zhang, Xin Zhang, Ying Li

et al.

Materials, Journal Year: 2023, Volume and Issue: 16(23), P. 7461 - 7461

Published: Nov. 30, 2023

The emergence of additive manufacturing, commonly referred to as 3D printing, has led a revolution in the field biofabrication. Numerous types bioprinting, including extrusion inkjet and lithography-based have been developed played pivotal roles driving multitude pioneering breakthroughs fields tissue engineering regenerative medicine. Among all bioprinting methods, light-based utilizes light crosslink or solidify photoreactive biomaterials, offering unprecedented spatiotemporal control over biomaterials enabling creation structures with extremely high resolution precision. However, lack suitable photoactive hindered application engineering. development only recently expanded. Therefore, this review summarizes latest advancements technologies, techniques, photo-initiators (PIs), their corresponding applications. Moreover, challenges facing are discussed, future directions proposed.

Language: Английский

Citations

13
Integration of 3D printed GelMA in rat liver: A pilot study DOI

S. Suresh,

Sachin J. Shenoy,

Anupama Sekar J

et al.

Journal of Bioactive and Compatible Polymers, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 18, 2025

Liver transplantation is the only treatment for patients with end-stage liver diseases yet limited availability of donor organs greatly restricts this option. One innovative approach to mitigate challenge involves biofabrication tissues through 3D Bioprinting technology. Gelatin Methacrylamide (GelMA), a modified form gelatin one extensively studied hydrogel bioprinting soft tissues. This study reports non-cytotoxic, printable bioink formulation composed 10% GelMA, 3% and 0.25% LAP (GelMA-G-L). We have in vivo integration printed construct within rat tissue following upto 30-days implantation period. Histological examination revealed that GelMA-G-L had successfully integrated surrounding tissue, displaying adequate vascularization no indications adverse immune responses or rejection signs.

Language: Английский

Citations

0
Advancing Precision Surgery: The Role of 3D Printing in Liver Surgery DOI
Tao Lan,

Yihe Dai,

Pingping Hu

et al.

3D Printing and Additive Manufacturing, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Language: Английский

Citations

0
Characterization of the Coating Layers Deposited onto Curved Surfaces Using a Novel Multi-Nozzle Extrusion Printer DOI Creative Commons
Ramses Seferino Trigo Torres, Lawrence Kulinsky, Arash Kheradvar

et al.

Micromachines, Journal Year: 2025, Volume and Issue: 16(5), P. 505 - 505

Published: April 26, 2025

Over the past two decades, additive manufacturing has advanced significantly, enabling rapid fabrication of functional components across various applications. In medical devices, it been used for prototyping, prosthetics, drug delivery platforms, and more recently, tissue scaffolding. However, current technologies face challenges, particularly in depositing conformal layers over curved surfaces. This study introduces a novel multi-nozzle extrusion printer concept designed to deposit soft gel onto A custom clearance locking mechanism enhances printer’s ability achieve coatings on both flat substrates. We investigate key deposition parameters, including displacement volume nozzle configuration, while comparing sequences: “Press Express” “Express Press”. Our results demonstrate that Press” technique yields uniform, merged than method. technology holds promise further refinement potential applications engineering.

Language: Английский

Citations

0
Recent Advances in Three-Dimensional In Vitro Models for Studies of Liver Fibrosis DOI
Kyun Yoo, Gyeongmin Kim, Jeong Sang Son

et al.

Tissue Engineering and Regenerative Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: May 13, 2025

Language: Английский

Citations

0