ACADEMIA JOURNAL OF BIOLOGY,
Journal Year:
2025,
Volume and Issue:
47(1), P. 33 - 43
Published: March 27, 2025
This
study
aimed
to
determine
the
gut
microbiota
composition
of
adult
Apis
mellifera
honeybees
from
bee
farms
in
Bac
Giang
province,
including
both
healthy
colonies
and
those
infected
with
Sacbrood
virus
(SBV).
The
SBV-infected
bees
was
assessed
using
next-generation
sequencing
(NGS)
V3-V4
region
16S
rRNA
gene
on
Illumina
MiSeq
system.
As
a
result,
NGS
analysis
identified
1,659
operational
taxonomic
units
(OTUs)
coverage
99%
an
average
read
length
430
bp.
results
revealed
that
harbored
four
microbial
phyla:
Proteobacteria
(48.44%),
Firmicutes
(38.65%),
Actinobacteria
(1.57%),
Bacteria_uc
(10.95%).
In
contrast,
group
consisted
three
(40.61%),
(45.55%),
(13.37%).
species
showed
shared
common
core
bacterial
species.
However,
Bifidobacterium_uc
Commensalibacter
AY370188_s
were
more
prevalent
significantly
reduced
bees.
Conversely,
Fructobacillus
fructosus
Lactobacillus
kunkeei
found
exclusively
These
lactic
acid
bacteria
(LAB)
have
been
shown
inhibit
growth
pathogenic
bacteria.
Our
findings
provide
valuable
scientific
foundation
for
developing
biological
products
improve
honeybee
health
disease
resistance.
Toxics,
Journal Year:
2023,
Volume and Issue:
11(2), P. 100 - 100
Published: Jan. 21, 2023
Microplastics
(MPs),
in
the
form
of
fragments
and
fibers,
were
recently
found
honey
samples
collected
Ecuador
as
well
bees
from
Denmark
China.
However,
little
is
known
about
how
MPs
impact
bee
health.
To
fill
this
knowledge
gap,
we
investigated
potential
toxicity
irregularly
shaped
polystyrene
(PS)-MP
on
In
first
experiment
its
kind
with
bees,
chronically
exposed
a
well-established
gut
microbiome
to
small
(27
±
17
µm)
or
large
(93
25
PS-MP
at
varying
concentrations
(1,
10,
100
µg
mL−1)
for
14
days.
Bee
mortality,
food
consumption,
body
weight
all
studied.
We
that
chronic
exposure
has
no
effect
survival,
but
reduced
feeding
rate
weight,
particularly
10
per
mL,
which
may
have
long-term
consequences
The
findings
study
could
assist
risk
assessment
pollinator
Ecology,
Journal Year:
2025,
Volume and Issue:
106(3)
Published: March 1, 2025
Abstract
Rising
temperatures
negatively
affect
bumble
bee
fitness
directly
through
physiological
impacts
and
indirectly
by
disrupting
mutualistic
interactions
between
bees
other
organisms,
which
are
crucial
in
determining
species‐specific
responses
to
climate
change.
Gut
microbial
symbionts,
key
regulators
of
host
nutrition
health,
may
be
the
Achilles'
heel
thermal
insects.
They
not
only
modulate
biotic
with
plants
pathogens
but
also
exhibit
varying
sensitivity
themselves.
Understanding
how
environmental
changes
disrupt
microbiome
communities
is
a
first
step
determine
potential
consequences
for
population
responses.
We
analyzed
gut
bacterial
six
species
inhabiting
different
climatic
niches
along
an
elevational
gradient
German
Alps
using
16S
ribosomal
DNA
amplicon
sequencing.
investigated
whether
inter‐
intraspecific
differences
can
linked
species'
niches,
differ
temperature,
flower
resource
composition,
likely
pathogen
pressure.
A
reciprocal
translocation
experiment
distinct
regions
tested
Bombus
terrestris
lucorum
change
short‐term
when
exposed
new
environments.
Finally,
we
these
heat
cold
wave
scenarios
within
chambers
disentangle
pure
temperature‐driven
effects
on
from
effects.
Interspecific
variation
composition
exceeded
variation.
Species
levels
stability,
where
stability
defined
as
within‐group
variance:
lower
indicated
greater
variance,
predominantly
observed
higher
elevations.
Transplanted
showed
subtle
adjustments,
marked
increase
Lactobacillaceae
upon
exposure
warmer
regions;
however,
microbiomes
did
under
laboratory
temperature
scenarios.
conclude
that
could
lead
For
example,
less
stable
elevations
might
indicate
increased
pathogens.
Short‐term
following
relatively
microbiomes,
such
B.
,
rapidly
integrate
bacteria,
their
capacity
cope
environments
While
foraging
for
nectar
and
pollen,
bees
are
exposed
to
a
myriad
of
xenobiotics,
including
plant
metabolites,
which
may
exert
wide
range
effects
on
their
health.
Although
the
bee
genome
encodes
enzymes
that
help
in
metabolism
it
has
lower
detoxification
gene
diversity
than
genomes
other
insects.
Therefore,
rely
components
shape
physiology,
such
as
microbiota,
degrade
potentially
toxic
molecules.
In
this
study,
we
show
amygdalin,
cyanogenic
glycoside
found
honey
bee-pollinated
almond
trees,
can
be
metabolized
by
both
members
gut
microbiota.
microbiota-deprived
bees,
amygdalin
is
degraded
into
prunasin,
leading
prunasin
accumulation
midgut
hindgut.
microbiota-colonized
hand,
even
further,
does
not
accumulate
gut,
suggesting
microbiota
contribute
full
degradation
hydrogen
cyanide.
vitro
experiments
demonstrated
bacteria
strain-specific
characteristic
particular
genus
or
species.
We
strains
Bifidobacterium
,
Bombilactobacillus,
Gilliamella
amygdalin.
The
mechanism
appears
vary
since
only
some
produce
an
intermediate.
Finally,
investigated
basis
wkB204,
strain
fully
degrades
overexpression
secretion
several
carbohydrate-degrading
enzymes,
one
hydrolase
family
3
(GH3).
expressed
GH3
Escherichia
coli
detected
byproduct
when
cell
lysates
were
cultured
with
supporting
its
contribution
degradation.
These
findings
demonstrate
host
act
together
metabolize
dietary
metabolites.
