The NEL Family of Bacterial E3 Ubiquitin Ligases

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(14), P. 7725 - 7725

Published: July 13, 2022

Some pathogenic or symbiotic Gram-negative bacteria can manipulate the ubiquitination system of eukaryotic host cell using a variety strategies. Members genera Salmonella, Shigella, Sinorhizobium, and Ralstonia, among others, express E3 ubiquitin ligases that belong to NEL family. These use type III secretion systems translocate these proteins into cells, where they will find their targets. In this review, we first introduce process consider various ways alter ligation machinery. We then focus on members family, expression, translocation, subcellular localization in cell, review what is known about structure proteins, function virulence symbiosis, specific

Language: Английский

---

Apple U-box-type E3 ubiquitin ligase MdPUB23 reduces cold-stress tolerance by degrading the cold-stress regulatory protein MdICE1

Horticulture Research, Journal Year: 2022, Volume and Issue: 9

Published: Jan. 1, 2022

Abstract Cold stress limits plant growth, geographical distribution, and crop yield. The MYC-type bHLH transcription factor ICE1 is recognized as the core positive regulator of cold-stress response. However, how protein levels are regulated remains to be further studied. In this study, we observed that a U-box-type E3 ubiquitin ligase, MdPUB23, positively response in apple. expression MdPUB23 increased at both transcriptional post-translational cold stress. Overexpression transgenic apple enhanced sensitivity Further study showed directly interacted with MdICE1, promoting ubiquitination-mediated degradation MdICE1 through 26S-proteasome pathway reducing MdICE1-improved tolerance Our results reveal regulates by mediating stability MdICE1. PUB23–ICE1 ubiquitination module may play role maintaining homeostasis preventing overreactions from causing damage plants. discovery regulatory provides insights for exploration cold-stress-response mechanisms.

Language: Английский

---

Post‐translational modifications in diabetic cardiomyopathy

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(7)

Published: March 17, 2024

The increasing attention towards diabetic cardiomyopathy as a distinctive complication of diabetes mellitus has highlighted the need for standardized diagnostic criteria and targeted treatment approaches in clinical practice. Ongoing research is gradually unravelling pathogenesis cardiomyopathy, with particular emphasis on investigating various post-translational modifications. These modifications dynamically regulate protein function response to changes internal external environment, their disturbance homeostasis holds significant relevance development chronic ailments. This review provides comprehensive overview common involved initiation progression including O-GlcNAcylation, phosphorylation, methylation, acetylation ubiquitination. Additionally, discusses drug strategies targeting key modification targets, such agonists, inhibitors PROTAC (proteolysis chimaera) technology that targets E3 ubiquitin ligases.

Language: Английский

---

Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 2, 2025

Language: Английский

---

Beyond Tumors: The Pivotal Role of TRIM Proteins in Chronic Non-Tumor Lung Diseases

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 1899 - 1910

Published: Feb. 1, 2025

While TRIM proteins are extensively studied in the context of lung tumors, their roles non-tumor chronic diseases remain underexplored. This review delves into emerging significance family pathogenesis idiopathic pulmonary fibrosis (IPF), asthma, obstructive disease (COPD), and hypertension (PH). modulate key pathological processes, including inflammation, fibrosis, cellular remodeling, contributing to progression. We highlight potential as biomarkers therapeutic targets, offering promising avenues for drug development these debilitating respiratory disorders. However, translation findings clinical applications faces significant challenges. These include dual functional nature proteins, context-dependent roles, complexity downstream signaling networks, limitations current strategies achieving tissue-specific targeting with minimal off-target effects. Addressing challenges will require innovative approaches interdisciplinary efforts unlock diseases.

Language: Английский

---

The ubiquitin-proteasome system and learning-dependent synaptic plasticity – A 10 year update

Neuroscience & Biobehavioral Reviews, Journal Year: 2023, Volume and Issue: 152, P. 105280 - 105280

Published: June 13, 2023

Language: Английский

---

Current and future directions of USP7 interactome in cancer study

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Journal Year: 2023, Volume and Issue: 1878(6), P. 188992 - 188992

Published: Sept. 27, 2023

Language: Английский

---

Complete set of the Atg8–E1–E2–E3 conjugation machinery forms an interaction web that mediates membrane shaping

Nature Structural & Molecular Biology, Journal Year: 2023, Volume and Issue: 31(1), P. 170 - 178

Published: Dec. 6, 2023

Language: Английский

---

RNF115 aggravates tumor progression through regulation of CDK10 degradation in thyroid carcinoma

Cell Biology and Toxicology, Journal Year: 2024, Volume and Issue: 40(1)

Published: Feb. 20, 2024

Abstract Background RING Finger Protein 115 (RNF115), a notable E3 ligase, is known to modulate tumorigenesis and metastasis. In our investigation, we endeavor unravel the putative function inherent mechanism through which RNF115 influences evolution of thyroid carcinoma (THCA). Methods We analyzed expression in THCA using Cancer Genome Atlas (TCGA) database. The influence on progression was evaluated both vitro vivo experimental approaches. protein regulated by identified bioinformatics analysis, its biological significance further explored. Results tissues cells, showed elevated levels. Enhanced fostered cell proliferation, tumor growth, exacerbation epithelial-mesenchymal transition (EMT) THCA, while also promoting lung Bioinformatics analysis cyclin-dependent kinase 10 (CDK10) as downstream target RNF115, found be ubiquitinated degraded cells. Functionally, overexpression CDK10 counteract promotion malignant phenotype induced RNF115. From mechanistic perspective, activated Raf-1 pathway enhanced cancer cycle degrading Conclusion triggers EMT, metastasis ubiquitinating CDK10. regulation may profoundly influenced this process.

Language: Английский

---

The cross talk of ubiquitination and chemotherapy tolerance in colorectal cancer

Journal of Cancer Research and Clinical Oncology, Journal Year: 2024, Volume and Issue: 150(3)

Published: March 23, 2024

Abstract Ubiquitination, a highly adaptable post-translational modification, plays pivotal role in maintaining cellular protein homeostasis, encompassing cancer chemoresistance-associated proteins. Recent findings have indicated potential correlation between perturbations the ubiquitination process and emergence of drug resistance CRC cancer. Consequently, numerous studies spurred advancement compounds specifically designed to target ubiquitinates, offering promising prospects for therapy. In this review, we highlight enzymes associated with chemoresistance chemotherapy via Wnt/β-catenin signaling pathway, epithelial–mesenchymal transition (EMT), cell cycle perturbation. addition, summarize application small that treatment, along significance targeting as therapies.

Language: Английский

---