Clinical and Experimental Obstetrics & Gynecology,
Journal Year:
2024,
Volume and Issue:
51(4), P. 90 - 90
Published: April 3, 2024
Background:
Recently,
the
utilization
of
sugar
as
a
cryoprotectant
has
garnered
significant
attention.
Sucrose
and
trehalose,
non-permeable
cryoprotectants,
can
effectively
regulate
osmotic
pressure
inside
outside
cells
while
maintaining
cell
membrane
stability
during
cryopreservation.
Furthermore,
it
been
observed
that
monosaccharides
sugars,
particularly
fructose,
exhibit
superior
efficacy
in
preserving
quality
frozen
substances
compared
to
disaccharides
trisaccharides
sugars.
Nevertheless,
there
is
currently
limited
research
assessing
cryopreservation
effects
sucrose,
fructose.
The
objective
this
experiment
identify
best
between
fructose
for
intact
rat
ovaries.
Methods:
Intact
ovaries
with
blood
vessels
were
obtained
from
20
adult
Lewis
female
rats
divided
accordingly
into
four
groups:
control
(non-vitrified),
trehalose.
ovarian
samples
subjected
stepped
exposure
subsequently
follicular
histological
analysis
using
light
electron
microscopy.
Ovarian
apoptosis
was
evaluated
by
BCL2-Associated
X
(BAX)
immunohistochemistry
Terminal
Deoxynucleotidyl
Transferase-Mediated
Deoxyuridine
Triphosphate
nick
End-Labeling
(TUNEL)
analyses.
Results:
sucrose
trehalose
groups
preserved
structure
better
than
group.
Additionally,
no
statistical
difference
total
follicle
number
groups,
but
percentage
apoptotic
group
significantly
lower
(p
<
0.01).
Conclusions:
perfusion
protocols
histology.
However,
least
changes
study
suggested
would
be
well-applied
Trends in Food Science & Technology,
Journal Year:
2023,
Volume and Issue:
139, P. 104116 - 104116
Published: July 23, 2023
Freezing
reduces
the
temperature
of
food
system
and
extends
shelf
life
food.
However,
inevitable
formation
ice
crystals
could
impact
quality.
Small-size
are
more
acceptable
in
frozen
foods
than
large
aggregates
with
irregular
shapes.
Undesired
not
only
change
structure
cause
serious
quality
deterioration,
including
texture
alteration,
nutrient
loss
off-flavour.
Ice-regulating
materials
have
been
proven
to
regulate
nucleation,
growth
recrystallization,
which
show
promising
applications
industry.
The
theoretical
information
on
formation,
particularly
nucleation
was
described.
Current
ice-regulating
materials,
such
as
active
proteins,
saccharides,
natural
deep
eutectic
solvents
(NADES),
their
mechanisms
regulating
were
summarized.
Based
damage
foodstuffs
different
microstructures,
improving
also
discussed.
showed
superior
actions
addition
alleviated
improved
by
accelerating
or
inhibiting
freezing.
Moreover,
current
challenges
future
perspectives
regulation
for
high-efficiency
freezing
proposed.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(5), P. 4656 - 4656
Published: Feb. 28, 2023
Cryopreservation
is
an
expanding
strategy
to
allow
not
only
fertility
preservation
for
individuals
who
need
such
procedures
because
of
gonadotoxic
treatments,
active
duty
in
dangerous
occupations
or
social
reasons
and
gamete
donation
couples
where
conception
denied,
but
also
animal
breeding
endangered
species.
Despite
the
improvement
semen
cryopreservation
techniques
worldwide
expansion
banks,
damage
spermatozoa
consequent
impairment
its
functions
still
remain
unsolved
problems,
conditioning
choice
technique
assisted
reproduction
procedures.
Although
many
studies
have
attempted
find
solutions
limit
sperm
following
identify
possible
markers
susceptibility,
research
this
field
required
order
optimize
process.
Here,
we
review
available
evidence
regarding
structural,
molecular
functional
occurring
cryopreserved
human
strategies
prevent
it
Finally,
results
on
(ARTs)
outcomes
use
spermatozoa.
Cryobiology,
Journal Year:
2025,
Volume and Issue:
118, P. 105195 - 105195
Published: Jan. 8, 2025
The
cryopreservation
of
human
spermatozoa
is
an
integral
part
cryobiology,
aiming
to
support
the
in-vitro
fertilization.
latter
relies
on
availability
as
much
possible
reproductively
active
spermatozoa,
whose
number
after
thawing
decreases
due
accompanied
freezing
injury
and
cytotoxicity
cryoprotectants.
An
innovative
option
circumvent
these
obstacles
make
interface
non-wettable,
by
coating
it
with
rapeseed
oil
soot
possessing
intrinsic
cryoprotective
properties,
delaying
ice
formation
possibly
providing
identical
rates
intracellular
dehydration
extracellular
crystallization.
It
may
mean
that
this
technique
can
reduce
or
avoid
need
harmful
agents,
but
reach
such
a
developmental
stage,
synergistic
effect
certified
cryoprotectants
cooling
velocities
efficiency
soot-mediated
sperm
must
be
clarified.
With
intention
address
research
gap,
we
reveal
slow
freezing/thawing
distinct
mixtures
three
(SpermFreeze™,
CryoSperm™
DMSO)
semen
nine
patients
equalizes
percent
survived
declines
their
curvilinear
velocity.
At
instant
(∼7-20
s)
thawing,
via
specially-designed
fabric-coated
sheet
metal
cryoboxes,
inclusion
10
%
DMSO
noxious,
post-thaw
motility
reaches
74-100
independently
solutes.
These
surprising
findings
are
ascribed
quasivitrified
semen,
complete
ensures
fraction
matching
from
equilibrium
phase
diagram,
eluding
osmotic
shocks
paving
path
for
future
replacement
classic
vitrification.
