Targeting AURKA with multifunctional nanoparticles in CRPC therapy DOI Creative Commons
Bin Deng, Bo Ke,

Qixing Tian

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Dec. 30, 2024

Castration-resistant prostate cancer (CRPC) presents significant therapeutic challenges due to its aggressive nature and poor prognosis. Targeting Aurora-A kinase (AURKA) has shown promise in treatment. This study investigates the efficacy of ART-T cell membrane-encapsulated AMS@AD (CM-AMS@AD) nanoparticles (NPs) a photothermal–chemotherapy–immunotherapy combination for CRPC. Bioinformatics analysis Cancer Genome Atlas-prostate adenocarcinoma (TCGA-PRAD) dataset revealed overexpression AURKA PCa, correlating with clinical outcomes. Single-cell RNA sequencing data from GEO database showed reduction immune cells Experimentally, T membrane-biomimetic NPs loaded inhibitor Alisertib chemotherapy drug DTX were synthesized characterized by dynamic light scattering transmission electron microscopy, showing good stability uniformity (average diameter: 158 nm). In vitro studies demonstrated that these inhibited CRPC proliferation, increased G2/M population, elevated apoptosis, confirmed γH2AX expression. vivo, CM-AMS@AD accumulated tumor tissues, significantly slowed growth, decreased improved environment, enhancing dendritic (DC) maturation increasing CD8 + /CD4 ratios. These findings suggest offer promising triple-combination therapy CRPC, integrating photothermal, chemotherapy, immunotherapy, potential future applications.

Language: Английский

Preparation of pH-Responsive Tanshinone IIA-Loaded Calcium Alginate Nanoparticles and Their Anticancer Mechanisms DOI Creative Commons
Tianying Ren, Jing Wang, Yingxin Ma

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(1), P. 66 - 66

Published: Jan. 6, 2025

Background: Tanshinone IIA (Tan IIA) is a lipophilic active constituent derived from the rhizomes and roots of Salvia miltiorrhiza Bunge (Danshen), common Chinese medicinal herb. However, clinical applications Tan are limited due to its poor solubility in water. Methods: To overcome this limitation, we developed calcium alginate hydrogel (CA) as hydrophilic carrier for IIA, which significantly improved solubility. We also prepared nanoparticles with pH-responsive properties explore their potential controlled drug delivery. The physicochemical IIA/CA were evaluated, including size, stability, release profile. utilized RNA sequencing further investigate underlying anticancer mechanisms nanoparticles. Results: demonstrated enhanced exhibited potent activity vitro. Additionally, showed promising behavior, beneficial applications. Further investigation into molecular revealed that effects mediated through apoptosis, ferroptosis, autophagy pathways. Conclusions: This study confirms while presenting an innovative approach enhance poorly soluble compound. use CA-based could be valuable strategy improving therapeutic efficacy cancer treatment.

Language: Английский

Citations

2
Nanodelivery System of Traditional Chinese Medicine Bioactive Compounds: Application in the Treatment of Prostate Cancer DOI Creative Commons
Bo Zou, Yan Long,

Rui‐Song Gao

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 135, P. 155554 - 155554

Published: March 29, 2024

The long history of clinical experience in China have confirmed the effectiveness traditional Chinese medicine (TCM) treating prostate cancer (PCa). Until now, several bioactive compounds with anti-PCa potential, such as curcumin, gallic acid, and quercetin, been extracted from TCM. Recent studies shown that encapsulating these TCM into nano-delivery system enhanced their bioavailability improved ability to target PCa tumors.

Language: Английский

Citations

9
Tanshinone IIA and hepatocellular carcinoma: A potential therapeutic drug DOI Creative Commons
Hu Li,

Pengbo Hu,

Yajun Zou

et al.

Frontiers in Oncology, Journal Year: 2023, Volume and Issue: 13

Published: Jan. 31, 2023

Because of its high prevalence and poor long-term clinical treatment effect, liver disease is regarded as a major public health problem around the world. Among them, viral hepatitis, fatty liver, cirrhosis, non-alcoholic (NAFLD), autoimmune are common causes inducements injury, play an important role in occurrence development hepatocellular carcinoma (HCC). Tanshinone IIA (TsIIA) fat soluble polyphenol Salvia miltiorrhiza that extracted from miltiorrhiza. strong biological activity (anti-inflammatory, antioxidant), it widely used Asia to treat cardiovascular diseases. In addition, TsIIA has shown significant anti-HCC previous studies. It not only anti proliferation pro apoptotic properties. can also anti-cancer by mediating variety signal pathways, including phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt)/rapamycin (mTOR), mitogen-activated protein (MAPK), nuclear factor kappa-B (NF-κB). This review reviews existing evidence molecular mechanism TsIIA's effect but liver-protective impact on fibrosis, NAFLD, other risk factors for cancer. we conducted network pharmacological analysis HCC further screen explore possible targets against carcinoma. expected provide theoretical basis anti-HCC-related drugs based TsIIA.

Language: Английский

Citations

18
Analyzing the research landscape: Mapping frontiers and hot spots in anti-cancer research using bibliometric analysis and research network pharmacology DOI Creative Commons
Qi Han, Zhongxun Li, Yang Fu

et al.

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 7, 2023

Introduction: Network pharmacology has emerged as a forefront and hotspot in anti-cancer. Traditional anti-cancer drugs are limited by the paradigm of "one cancer, one target, drug," making it difficult to address challenges recurrence drug resistance. However, main advantage network lies its approach from perspective molecular relationships, employing arrow, multiple targets" strategy, which provides novel pathway for developing drugs. This study employed bibliometric analysis method examine pharmacology's application research progress cancer treatment January 2008 May 2023. will contribute revealing hotspots, offering new insights methodologies future investigations. Methods: We conducted literature search on (NPART) 2023, utilizing scientific databases such Web Science Core Collection (WoSCC) PubMed retrieve relevant articles reviews. Additionally, we visualization tools Citespace, SCImago Graphica, VOSviewer perform analysis. Results: encompassed 3,018 articles, with 2,210 WoSCC 808 PubMed. Firstly, an annual national publication trends citation counts indicated that China United States primary contributing countries this field. Secondly, recent keyword revealed emerging hotspots "tumor microenvironment," "anti-cancer drugs," "traditional Chinese medicine (TCM). " Furthermore, clustering demonstrated "calycosin," "molecular mechanism," docking," agents" were widely recognized areas garnering significant attention citations Ultimately, analyzed NPART challenges. Conclusion: represents first comprehensive paper based methods, aiming investigate research. The findings facilitate researchers swiftly comprehending current domain

Language: Английский

Citations

18
Enhancing Curcumol Delivery through PD-1 Targeted Nanocarriers: A Novel Therapeutic Approach for Prostate Cancer DOI
Hao Zhang, Yang Du, Xujia Liu

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 141, P. 156595 - 156595

Published: March 2, 2025

Language: Английский

Citations

0
Insights into emerging mechanisms of ferroptosis: new regulators for cancer therapeutics DOI Creative Commons
Siyi Xu,

Shuangshuang Yin,

Lei Wang

et al.

Cell Biology and Toxicology, Journal Year: 2025, Volume and Issue: 41(1)

Published: March 25, 2025

Ferroptosis is an iron-dependent form of regulated cell death characterized by the accumulation lipid peroxides, which has been implicated in pathogenesis various diseases, and therapeutic agents targeting ferroptosis are emerging as promising tools for cancer treatment. Current research reveals that ferroptosis-targeted therapies can effectively inhibit tumor progression or delay development. Notably, natural product-derived compounds-such artemisinin, baicalin, puerarin, quercetin, kaempferol, apigenin-have demonstrated ability to modulate ferroptosis, offering potential anti-cancer benefits. Mechanistically, exhibits negative glutathione peroxidase 4 (GPX4) regulation demonstrates a positive correlation with plasma membrane polyunsaturated fatty acid (PUFA) abundance. Moreover, labile iron pool (LIP) serves redox engine ferroptosis. This review systematically analyzes hallmarks, signaling pathways, molecular mechanisms focus on how small molecules regulate this process. It further evaluates their inducers inhibitors anti-tumor therapy, providing foundation future clinical translation.

Language: Английский

Citations

0
Importance of Advanced Detection Methodologies from Plant Cells to Human Microsystems Targeting Anticancer Applications DOI Open Access
Mostafa Gouda, Eman Ramadan Elsharkawy, Yong He

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(10), P. 4691 - 4691

Published: May 14, 2025

The growing global demand for phytochemicals as bioactive sources is prompting scientists to develop methods that link their sensory properties mechanisms of action in cancer treatment. Recent techniques tracking the actions small plant metabolites (SPMs) from single-cell molecular anticancer biomarkers could provide valuable insights this field. Among critical discussed review are real-time cell components through stable isotope probing (Sis) and microspectroscopy, which has attracted attention biotechnologists. Additionally, precise pathways required studying new into functional materials discussed, based on high-resolution accurate technologies, aid categorization. Notably, molecules under study have recently garnered applications due advancements effective evaluation surpass traditional methods. In December 2020, Food Drug Administration (FDA) authorized 89 SPMs safe natural molecules. conclusion, by combining spatiotemporal SPMs’ mechanisms, they facilitate development more exceptional, bio-efficient materials.

Language: Английский

Citations

0
Tanshinone I Stimulates Pyroptosis of Cisplatin-Resistant Gastric Cancer Cells by Activating the NF-κB/Caspase-3(8)/GSDME Signaling Pathway DOI
Guijun Wang, Yanrong Li,

Zhaokai Guo

et al.

DNA and Cell Biology, Journal Year: 2024, Volume and Issue: 43(4), P. 185 - 196

Published: March 11, 2024

Cisplatin (DDP) resistance frequently occurs in gastric cancer (GC) therapy. Tanshinone I is a liposoluble phenanthraquinone compound present the roots of

Language: Английский

Citations

3
Tanshinone analog inhibits castration-resistant prostate cancer cell growth by inhibiting glycolysis in an AR-dependent manner DOI Creative Commons
Jia Yu, Shengyou Li, Sha Cheng

et al.

Journal of Biological Chemistry, Journal Year: 2024, Volume and Issue: 300(4), P. 107139 - 107139

Published: March 5, 2024

Androgen receptor (AR) is one of the key targets for treatment Castration-resistant prostate cancer (CRPC). Current endocrine therapy can greatly improve CRPC patients. However, with change pathogenic mechanism, acquired resistance often leads to failure treatment. Studies have shown that tanshinone IIA (TS-IIA) and its derivatives significant antitumor activity, certain AR targeting effects, but mechanism unknown. In this study, TS-IIA analog TB3 was found significantly inhibit growth in vitro vivo. Molecular docking, cellular thermal shift assay CHX experiments confirmed target promoted degradation AR. Furthermore, glycolysis metabolism by AR/PKM2 axis..The addition pyruvic acid could alleviate inhibitory effect on cells. Besides, knockdown or PKM2 also reverse Taken together, our study suggests derivative inhibits prevent process axis.

Language: Английский

Citations

3
Dihydrotanshinone I inhibits gallbladder cancer growth by targeting the Keap1-Nrf2 signaling pathway and Nrf2 phosphorylation DOI Creative Commons
Zhuang Li,

Rong-Liang Mo,

Jun-feng Gong

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 129, P. 155661 - 155661

Published: April 21, 2024

Gallbladder cancer (GBC) poses a significant risk to human health. Its development is influenced by numerous factors, particularly the homeostasis of reactive oxygen species (ROS) within cells. This crucial for tumor cell survival, and abnormal regulation ROS associated with occurrence progression many cancers. Dihydrotanshinone I (DHT I), biologically effective ingredient isolated from salvia miltiorrhiza, has exhibited cytotoxic properties against various cells inducing apoptosis. However, precise molecular mechanisms which dht exerts its effects remain unclear. To explore anti-tumor impact on GBC elucidate potential mechanisms. The proliferation cells, NOZ SGC-996, was assessed using assays, including CCK-8 assay, colony formation assay EdU staining. We also examined apoptosis, cycle progression, levels, alterations in mitochondrial membrane delve into intricate mechanism. Quantitative PCR (qPCR), immunofluorescence staining, Western blotting were performed evaluate target gene expression at both mRNA protein levels. correlation between nuclear factor erythroid 2-related 2 (Nrf2) kelch-like ECH-associated 1 (Keap1) co-immunoprecipitation. Finally, vivo effect investigated xenograft model gallbladder mice. Our research findings indicated that exerted inhibiting proliferation, disrupting potential, oxidative stress studies substantiated inhibition growth nude Mechanistically, primarily targeted Nrf2 promoting Keap1 mediated degradation kinase C (PKC) induced phosphorylation. leads suppression translocation reduction expression. Moreover, overexpression effectively counteracted I, while knockdown significantly enhanced inhibitory GBC. Meanwhile, PKC inhibitors import increased sensitivity treatment. Conversely, activators, proteasome inhibitors, antioxidants activators all antagonized apoptosis generation SGC-996 inhibited regulating Keap1-Nrf2 signaling pathway These insights provide strong rationale further investigation as therapeutic agent

Language: Английский

Citations

3