Exploring the Effects of Different Drying Methods on Related Differential Metabolites of Pleurotus citrinopileatus Singer Based on Untargeted Metabolomics

Plants, Journal Year: 2024, Volume and Issue: 13(12), P. 1594 - 1594

Published: June 7, 2024

Singer (PCS) has attracted increasing attention as a raw material for medicine and food. Its quality is greatly affected by the accumulation of metabolites, which varies with applied drying methods. In this study, we utilize an approach based on ultra-high-performance liquid chromatography/Q Exactive mass spectrometry (UHPLC-QE-MS) to reveal metabolic profiles PCS from three different methods (natural air-drying, NAD; hot-air-drying, HAD; vacuum freeze-drying, VFD). The results showed that lipids, amino acids their derivatives were all important secondary metabolites produced during NAD, HAD VFD treatments, key differential including fifteen lipids seven acids. Meanwhile, was best way long-term preservation dried PCS. Hot-drying methods, especially HAD, can improve medicinal component Furthermore, KEGG enrichment analysis highlighted 16 pathways indicated acid metabolism might be metabolite pathway process. Our study elucidates relationship between or determine mechanisms affecting PCS, finally provides reference values further development application in functional food medications.

Language: Английский

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Unveiling the Potential Prebiotic Effects of Edible Mushroom Pleurotus djamor During In Vitro Colonic Fermentation

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 21, 2024

The ability of edible mushrooms to modulate the intestinal microbiota is a topic interest. This study shows that digested Pleurotus djamor powder (MUS) exhibits prebiotic effects during an in vitro colonic fermentation. Phenolic compounds, including epicatechin (3.03 ± 1.54 mg/L), gallic acid (2.71 and quercetin 3-glucoside (2.40 were found P. djamor. MUS significantly increased relative abundance Lactobacillus spp./Enterococcus spp. (1.12% – 4.83%), Bifidobacterium (0.59% 1.85%), Ruminococcus albus/R. flavefaciens (0.37% 1.88%), reduced Clostridium histolyticum (2.89% 1.22%) 48 enhanced lactic short-chain fatty production decreased pH levels. 1H NMR analysis revealed presence essential amino acids, branched-chain other compounds benefit human health. results indicate on microbiota.

Language: Английский

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Allobaculum plays a predominant role in the activation of brown fat tissue by diallyl trisulfide

Food Frontiers, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Abstract Diallyl trisulfide (DATS) is a bioactive compound in garlic. It has been reported to ameliorate obesity by preventing adipogenesis and lipogenesis white adipose tissue. But we found DATS could also activate brown tissue (BAT) high‐fat diet (HFD)‐fed mice, suggesting another pathway obesity. To explore the mechanism of activating BAT, mice fed with HFD were treated DATS. The results showed that significantly activated BAT regulated gut microbiota. Next, microbiota transplantation (GMT) was carried out, it effects on transferred GMT. Further, analyzed bacteria consistent alteration DATS‐treated GMT recipient screened. Allobaculum promoted different doses GMT, may be potential factor mediating Finally, oral supplementation stercoricanis , primary species confirmed A. Additionally, live pasteurized compared revealed pasteurization did not diminish beneficial which indicates inactivated bacterial cells or cellular components are responsible for . This study reveals promotes thermogenesis regulating bacterium provides theoretical support application treatment

Language: Английский

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Overview of Pleurotus spp., edible fungi with various functional properties

International Food Research Journal, Journal Year: 2023, Volume and Issue: 30(5), P. 1074 - 1092

Published: Oct. 24, 2023

Pleurotus fungi, also known as basidiomycetous have been a part of human culture for thousands years. They exhibit anticancer, antitumor, antibacterial, and immunomodulatory effects, having biotechnological, medicinal, aesthetic applications. are versatile, highly resistant to illnesses pests, do not require special growing conditions. These properties make them readily marketable, can be found in supermarkets worldwide, generating multimillion-dollar sale revenues. The global edible mushroom market was valued at USD 5.08 billion 2021, which is expected grow 6.43 2028. China produces about 87% spp. globally; other Asian countries generate 12%, Europe America account approximately 1%. distinct functional characteristics, including high protein content with proper essential amino acid score pattern, dietary fibre profile, amounts vitamins (e.g., B D) minerals Fe, Zn, Cu, Se), low fat. Therefore, provide alternative industrial tools. present review discusses biotechnological tools acquiring metabolites interest, studying them, analysing bioactive substances that used various fields, medicine food.

Language: Английский

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The Short-Term Variation of Human Gut Mycobiome in Response to Dietary Intervention of Different Macronutrient Distributions

Nutrients, Journal Year: 2023, Volume and Issue: 15(9), P. 2152 - 2152

Published: April 29, 2023

While the human gut is home to a complex and diverse community of microbes, including bacteria fungi, research on microbiome has largely focused bacteria, with relatively little attention given mycobiome. This study aims investigate how diets different dietary macronutrient distributions impact We investigated mycobiome response high-carbohydrate, low-fat (HC) low-carbohydrate high-fat (LC) diet interventions based series 72-day feeding-based n-of-1 clinical trials. A total 30 participants were enrolled underwent three sets HC LC in randomized sequence. Each set lasted for 24 days 6-day washout period between interventions. collected analyzed fungal composition 317 stool samples before after each intervention period. To account intra-individual variation across sets, we averaged data from repeated analysis. Of participants, 28 (aged 22–34 years) completed entire intervention. Our results revealed significant increase alpha diversity (p < 0.05) changes (beta diversity, p Specifically, observed enrichment five genera (Pleurotus, Kazachstania, Auricularia, Paraphaeosphaeria, Ustilaginaceae sp.; FDR 0.052) depletion one genus (Blumeria; = 0.03) After intervention, was enriched (Ustilaginaceae 0.003), depleted (Blumeria, Agaricomycetes spp., Malassezia, Rhizopus, Penicillium; 0.1). provides novel evidence structure change reveals diet-specific genera.

Language: Английский

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Modulatory Effects of Lactarius hatsudake on Obesity and Gut Microbiota in High-Fat Diet-Fed C57BL/6 Mice

Foods, Journal Year: 2024, Volume and Issue: 13(6), P. 948 - 948

Published: March 20, 2024

Lactarius hatsudake (LH), a great wild endemic fungus, contains rich nutritional components with medicinal properties. The effects of LH on body weight, liver injury, blood lipids, and gut microbiota in C57BL/6 mice fed high-fat diet (HFD) for 8 weeks was examined this research. Though there no clear impact weight loss, the findings indicate that treatment effectively decreased damage caused by HFD, as well lowered serum total cholesterol, triacylglycerol, low-density lipoprotein cholesterol levels. Additionally, it positively influenced to resemble normal diet. In HFD-fed mice, markedly boosted levels Parabacteroides, unclassified Muribaculaceae, Oscillibacter, Oscillospiraceae, while reducing abundance Lachnospiraceae NK4A136 group Erysipelatoclostridium, ratio Firmicutes Bacteroidetes. Further analysis correlation possible connection between obesity microbiota. Obesity-related indices show positive association Eubacterium coprostanoligenes group, Blautia, displaying negative Clostridia vadinBB60 Helicobacter, Ruminococcaceae, Oscillospiraceae. results suggest can help combat may have potential be utilized functional food.

Language: Английский

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Experimental Models in Unraveling the Biological Mechanisms of Mushroom-Derived Bioactives against Aging- and Lifestyle-Related Diseases: A Review

Nutrients, Journal Year: 2024, Volume and Issue: 16(16), P. 2682 - 2682

Published: Aug. 13, 2024

Mushrooms have garnered considerable interest among researchers due to their immense nutritional and therapeutic properties. The presence of biologically active primary secondary metabolites, which includes several micronutrients, including vitamins, essential minerals, other dietary fibers, makes them an excellent functional food. Moreover, the inclusion mushrooms has been reported reduce incidence aging- lifestyle-related diseases, such as cancer, obesity, stroke, well provide overall health benefits by promoting immunomodulation, antioxidant activity, enhancement gut microbial flora. multifunctional activities mushroom extracts evaluated both in vitro vivo studies using cell lines along with invertebrate vertebrate model systems address human diseases disorders at molecular levels. Although each its own strengths lacunas, various generated a plethora data regarding regulating players that are modulated order protective activities; hence, this review intends compile overview plausible mechanism action mushroom-derived bioactives, will be helpful future medicinal explorations.

Language: Английский

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Regulating role of Pleurotus ostreatus insoluble dietary fiber in high fat diet induced obesity in rats based on proteomics and metabolomics analyses

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 136857 - 136857

Published: Oct. 1, 2024

Language: Английский

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Gut microbial change after administration of Lacticaseibacillus paracasei AO356 is associated with anti-obesity in a mouse model

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 29, 2023

The status of an impaired gut microbial community, known as dysbiosis, is associated with metabolic diseases such obesity and insulin resistance. use probiotics has been considered effective approach for the treatment prevention related dysbiosis. anti-obesity effect Lacticaseibacillus paracasei AO356 was recently reported. However, L. on microbiota not yet identified. This study aimed to elucidate ensure its safety a probiotic.Oral administration (107 colony-forming units [CFU]/mg per day, 5 days week, 10 weeks) mice fed high-fat diet significantly suppressed weight gain fat mass. We investigated composition explored association obesity-related markers.Oral changed modified relative abundance Lactobacillus, Bacteroides, Oscillospira. Bacteroides Oscillospira were lipid metabolism pathway markers. also confirmed using antibiotics resistance, hemolysis activity, bile salt hydrolase lactate production, toxicity tests following assessment guidelines Ministry Food Drug Safety (MFDS).This demonstrated that only but changes in pathways obesity. Furthermore, overall seen this could increase potential new probiotic materials effects.

Language: Английский

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Characterization of the Cafeteria Diet as Simulation of the Human Western Diet and Its Impact on the Lipidomic Profile and Gut Microbiota in Obese Rats

Nutrients, Journal Year: 2022, Volume and Issue: 15(1), P. 86 - 86

Published: Dec. 24, 2022

The obesity pandemic has been strongly associated with the Western diet, characterized by consumption of ultra-processed foods. lifestyle causes gut dysbiosis leading to impaired fatty acid metabolism. Therefore, this study aimed evaluate shifts in microbiota and correlate these serum profiles male Wistar rats fed a cafeteria diet. Ten were standard diet (CTL, n = 5) (CAF, for fifteen weeks. Body weight food intake recorded once three times per week, respectively. At end study, fresh fecal samples collected, tissues removed, obtained further analyses. Gut was analyzed sequencing V3-V4 region 16S rRNA gene. Serum fractioned quantified via gas chromatography. CAF induced an obese phenotype accompanied acids, finding significantly higher proportions total saturated acids (SFAs) C20:3 n-6, lower C18:1 n-7 C18:3 n-3 phospholipid (PL) fraction. Furthermore, circulating C10:0, decreased monounsaturated (MUFAs), including oleic n-9, increased cholesterol ester (CE) metabotype may be mediated caused rich C16:0, C18:0, n-9 C18:2 n-6 resulting 34:1 omega-6/omega-3 ratio. C10:0 several genera bacteria such as Prevotella (positive) Anaerotruncus (negative). Two classes Firmicutes, Bacilli Erysipelotrichi, positively correlated PL- CE- 18:1 TM7 Bacteroidetes inversely PL-SFAs 18:2

Language: Английский

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