The Effect of In Vitro Digestion on the Anti-allergic, Anti-Inflammatory and Antioxidant Properties of Purple Rice and Purple Barley Phenolic Extracts in Caco-2 and RBL-2H3 Cells

Food Bioscience, Journal Year: 2024, Volume and Issue: 61, P. 104943 - 104943

Published: Aug. 16, 2024

Gastrointestinal diseases are associated with increased oxidative stress and inflammation. Pigmented cereal polyphenols believed to have therapeutic potential. However, the impact of digestion on their bioactivity has not been fully elucidated. This study investigated ability purple rice extract (PRx) barley (PBx) alleviate stress-induced intestinal epithelial cell death allergic The used a human colon adenocarcinoma (Caco-2) line simulate for 4 h using 1 mM hydrogen peroxide. To evaluate protective effects digested undigested extracts, cells were first pre-treated varying concentrations (50, 100, 200, 500 μg/ml) both extracts. A rat basophilic leukemia (RBL-2H3) was investigate extracts prevent calcium ionophore-induced histamine cytokine release. results demonstrated that phenolic prevented in Caco-2 reduced Interleukin (IL)-8 secretion. PRx showed greater cytoprotective than PBx. Both time-dependent antihistamine effect, greatest inhibitory observed after 2 treatment PRx. Additionally, significantly attenuated release several cytokines including IL-2, IL-4, IL-13, monocyte chemoattractant protein-1, interferon-gamma, tumour necrosis factor-alpha. Overall, demonstrates pigmented potential sources which may promote health

Language: Английский

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An Overview of the Influence of Breastfeeding on the Development of Inflammatory Bowel Disease

Nutrients, Journal Year: 2023, Volume and Issue: 15(24), P. 5103 - 5103

Published: Dec. 13, 2023

The first 1000 days of life is a critical period that contributes significantly to the programming an individual's future health. Among many changes occur during this early in life, there growing evidence establishment healthy gut microbiota plays important role prevention both short- and long-term health problems. Numerous publications suggest quality colonisation depends on several dietary factors, including breastfeeding. In respect, relationship between breastfeeding risk inflammatory bowel disease (IBD) has been suggested. IBDs are chronic intestinal diseases, perinatal factors may be partly responsible for their onset. We review existence links IBD based experimental clinical studies. Overall, despite encouraging data rodents, association development remains controversial humans, due considerable heterogeneity duration exclusive probably decisive its lasting effect IBD. Thus, specific improvements our knowledge could support interventions targeting microbiome, such as use prebiotics, probiotics or postbiotics, order prevent disease.

Language: Английский

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Investigating the Crime Scene—Molecular Signatures in Inflammatory Bowel Disease

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(13), P. 11217 - 11217

Published: July 7, 2023

Inflammatory bowel diseases (IBD) are without cure and troublesome to manage because of the considerable diversity between patients lack reliable biomarkers. Several studies have demonstrated that diet, gut microbiota, genetics other patient factors essential for disease occurrence progression. Understanding link these is crucial identifying molecular signatures identify biomarkers advance management IBD. Recent technological breakthroughs data integration fuelled intensity this research. This research demonstrates effect diet depends on microbial activity. It also identifies a range potential IBD management, including mucosa-derived cytokines, gasdermins neutrophil extracellular traps, all which need further evaluation before clinical translation. review provides an update cutting-edge in aims improve quality life.

Language: Английский

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Ketogenic Diet Protects from Experimental Colitis in a Mouse Model Regardless of Dietary Fat Source

Nutrients, Journal Year: 2024, Volume and Issue: 16(9), P. 1348 - 1348

Published: April 29, 2024

While ketogenic diets (KDs) may have potential as adjunct treatments for gastrointestinal diseases, there is little knowledge on how the fat source of these impacts intestinal health. The objective this study was to investigate dietary KD influences experimental colitis. We fed nine-week-old male C57BL/6J mice (n = 36) with a low-fat control diet or high either in saturated fatty acids (SFA-KD) polyunsaturated linoleic acid (LA-KD) four weeks and then induced colitis dextran sodium sulfate (DSS). To compare diets, we analyzed macroscopic histological changes colon, permeability fluorescein isothiocyanate−dextran (FITC–dextran), colonic expression tight junction proteins inflammatory markers. effects were more pronounced LA-KD, both KDs markedly alleviated DSS-induced lesions. LA-KD prevented inflammation-related weight loss shortening well preserved Il1b Tnf at healthy level. Despite no significant between-group differences FITC–dextran, mitigated protein expression. Thus, preventive against inflammation, level effect being dependent source.

Language: Английский

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The beneficial impact of ketogenic diets on chemically-induced colitis in mice depends on the diet's lipid composition

The Journal of Nutritional Biochemistry, Journal Year: 2024, Volume and Issue: 134, P. 109736 - 109736

Published: Aug. 10, 2024

Previously, we showed that restrictive diets, including ketogenic diet (KD), have an anti-inflammatory impact on the healthy gastrointestinal tract of mice. Afterward, found energy-restricting diets mitigate inflammation in dextran sodium sulfate (DSS) colitis mouse model. The current study aimed to verify KD DSS and assess if diet's fat composition influences outcomes intervention. Mice with mild chronic were fed control chow, composed long-chain triglycerides (KD LCT) or a containing mix LCT medium-chain LCT/MCT). KDs did not reverse DSS-enhanced gut permeability shortening colon. Both had similar liver, cecum, spleen weight, villi colon length, thickness muscularis externa, expression ZO-1 occludin. On contrary, body glutathione (GSH) taurine-GSH levels, GSH-S transferase (GST), myeloperoxidase (MPO) activity, as well abundance several fecal bacteria, all differentially affected by two types KDs. When compared diet, reduction mucosa cytokines was stronger than LCT/MCT group. We conclude interventions terms potential therapeutical applications depend lipid composition. strong positive inflammation. A contribution GSH correlation between MPO activity microbiota identified.

Language: Английский

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The Hydrophobic Amino Acid-Rich Fish Collagen Peptide Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice via Repairing the Intestinal Barrier, Regulating Intestinal Flora and AA Metabolism

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(46), P. 25690 - 25703

Published: Nov. 8, 2024

The incidence of ulcerative colitis (UC) is increasing annually, but treatment option limited. Fish collagen peptide (FCP) a food source that has shown promise in alleviating UC symptoms. However, its impact on the intestinal barrier and metabolic homeostasis remains unclear. This study aimed to analyze sequences absolute amino acid (AA) content FCP, assessing effects mice induced by dextran sulfate sodium (DSS). FCP was examined liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis. 3% DSS utilized induce murine models, followed assessment therapeutic efficacy FCP. Clinical manifestations were meticulously evaluated scored. Subsequently, samples procured for histological examination epithelial integrity analysis as well macrogenomic metabolomic profiling. Here, it shows abundant AAs particularly enriched hydrophobic (HAAs). Furthermore, observed effectively reversed colon shortening reduced extent damage. Additionally, suppressed abnormal expression inflammatory factors proteins modulated dysbiosis gut microbiota toward balanced state. These alterations led activation alkaline AA various metabolisms, ultimately contributing mitigation In summary, diverse high rich HAAs, can alleviate DSS-induced via preserving integrity, regulating microbiota, modulating metabolism.

Language: Английский

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Restrictive diets have a beneficial impact on dextran sodium sulfate-induced colitis in male mice

Journal of Nutrition, Journal Year: 2023, Volume and Issue: 154(1), P. 121 - 132

Published: Nov. 10, 2023

Language: Английский

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Titanium dioxide nanoparticles induce energy perturbation by stimulating glycolytic metabolic profile in rats

Life Sciences Medicine and Biomedicine, Journal Year: 2024, Volume and Issue: 8(1)

Published: June 19, 2024

The safety of titanium dioxide nanoparticles (TiO2-NPs) remains uncertain due to a scarcity data regarding its absorption, distribution, elimination, and potential adverse effects following oral exposure. As emerging evidence suggests perturbations in energy metabolism play pivotal role the toxicity induced by various toxicants, this investigation aimed assess effect TiO2-NPs on activities specific glycolytic enzymes rats. In study, seventy-two (72) male Wistar rats (200 ± 20g) were divided into 12 groups, each consisting 6 animals. orally exposed (8-12nm) at doses 50, 150, 250 mg/kg body weight (BW) for durations 4, 8, weeks. control groups received distilled water. results showed that, except 150 BW dose weeks, exposure led an up-regulation hexokinase activity lymphocytes, plasma, erythrocytes, liver. Hepatic lymphocyte aldolase also up-regulated, 8 weeks 50 doses, where slight decreases observed. Plasma increased, dose, while erythrocyte increased only during 4 8-week but decreased throughout 12-week Notably, compared lactate dehydrogenase activity, which decreased, consistent pattern observed hepatic was down-regulation TiO2-NPs. other instances, time intervals, up-regulated. from study underscores that can disrupt pathway metabolism, particularly initial These are characterized

Language: Английский

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Role of diet in primary and secondary prevention of periodontitis and non-specific inflammatory bowel diseases. Part II

Annals of Agricultural and Environmental Medicine, Journal Year: 2024, Volume and Issue: 31(2), P. 170 - 177

Published: June 23, 2024

1. Goździewska M, Łyszczarz A, Kaczoruk Kolarzyk E. Relationship between periodontal diseases and non-specific inflammatory bowel – an overview. Part I. Ann Agric Environ Med. 2024;31(1):1–7. https://doi.org/10.26444/aaem/.... CrossRef Google Scholar

Language: Английский

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Key Interleukins in Inflammatory Bowel Disease—A Review of Recent Studies

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 121 - 121

Published: Dec. 26, 2024

Inflammatory bowel disease (IBD) is an immune disorder of the gastrointestinal tract with a complex aetiopathogenesis, whose development influenced by many factors. The prevalence IBD increasing worldwide, in both industrialized and developing countries, making global health problem that seriously affects quality life. In 2019, there were approximately 4.9 million cases worldwide. Such large number patients entails significant healthcare costs. treatment IBD, current therapeutic target mucosal healing, as intestinal inflammation often persists despite resolution abdominal symptoms. Treatment strategies include amino salicylates, corticosteroids, immunosuppressants, biologic therapies focus on reducing inflammation, inducing prolonging remission, treating complications. American College Gastroenterology (ACG) guidelines also indicate nutritional may be considered addition to other therapies. However, approaches are not fully effective associated various limitations, such drug resistance, variable efficacy, side effects. As chronic accompanies characterized infiltration variety cells increased expression pro-inflammatory cytokines, including IL-6, TNF-α, IL-12, IL-23 IFN-γ, new mainly targeting pathways. Interleukins one molecular targets therapy. related cytokines serve means communication for innate adaptive cells, well nonimmune tissues. These play important role pathogenesis course them promising future our work, we review scientific studies published between January 2022 November 2024 describing most interleukins involved IBD. Some papers present data precise individual New clinical have been provided, particularly blocking interleukin 23 1beta. addition, several use different described recent years.

Language: Английский

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