New insight on antioxidants and anti-obesity properties of two seagrasses Thalassia hemprichii and Zostera marina: an integrated molecular docking simulation with in vitro study

F1000Research, Journal Year: 2024, Volume and Issue: 12, P. 727 - 727

Published: April 8, 2024

Background The oceans are teeming with a diverse range of marine organisms that offer unique health benefits, such as seagrass which is one many key products have garnered attention for their potential therapeutic properties. However, until now there been few successful reports seagrass’s metabolites profile and biological activity. Therefore, this work aims to or chemical constituents assess the antioxidants anti-obesity effects two seagrasses, Thalassia hemprichii Zostera marina. Methods Once authenticated, T. Z. marina were extracted different solvents, polar (ethanol) nonpolar (hexane). Metabolite profiling was performed using untargeted metabolomic via liquid chromatography coupled high-resolution tandem mass spectrometry method analysis, then antioxidant capabilities assessed by molecular docking in vitro studies on selected receptors. Results A total 9 11 observed from continued docking. Some compounds promising inhibitors human inducible nitric oxide synthase, reactive oxygen species (ROS) 1 kinase, pancreatic lipase, fat obesity-associated (FTO) proteins, including luteolin, 6-hydroxy luteolin O-glucoside, luteolin-O-sulphate, Thalassiolin A, C, kaempferol-7,4′-dimethylether-3-O-sulfate, apigenin, diosmetin. ethanol extract (THE) EC50 value shows ABTS radical scavenging activity 76.00 μg/mL, smaller than standard controls (Trolox, 76.54 μg/mL) followed lipase inhibition THE has same pattern (EC50 < Orlistat). Conclusions This concludes seagrasses candidates functional food and/or drugs in combating free radicals obesity.

Language: Английский

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A Comparative Analysis on Impact of Extraction Methods on Carotenoids Composition, Antioxidants, Antidiabetes, and Antiobesity Properties in Seagrass Enhalus acoroides: In Silico and In Vitro Study

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(8), P. 365 - 365

Published: Aug. 12, 2024

Enhalus acoroides, a tropical seagrass, is known for its significant contribution to marine ecosystems and potential health benefits due bioactive compounds. This study aims compare the carotenoid levels in E. acoroides using green extraction via ultrasound-assisted (UAE) microwave-assisted (MAE) evaluate biological properties of these extracts against oxidative stress, diabetes, obesity through silico vitro analyses. samples were collected from Manado City, Indonesia, subjected UAE MAE. The analyzed UHPLC-ESI-MS/MS identify carotenoids, including β-carotene, lutein, lycopene, β-cryptoxanthin, zeaxanthin. In analysis was conducted predict compounds’ bioactivity, toxicity, drug-likeness WAY2DRUG PASS molecular docking with CB-Dock2. compounds C3, C4, C7 demonstrated notable interactions, key metabolic proteins microRNAs, further validating their therapeutic benefits. assays evaluated antioxidant activities DPPH FRAP assays, antidiabetic α-glucosidase α-amylase inhibition, antiobesity effects lipase inhibition MTT assay 3T3-L1 cells. Results indicated that both MAE exhibited antioxidant, antidiabetic, activities. showed higher content greater activity compared extracts. These findings suggest mainly when extracted MAE, has promising as source natural developing marine-based agents. supplements existing literature by providing insights into efficient methods carotenoids.

Language: Английский

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DDAH1 recruits peroxiredoxin 1 and sulfiredoxin 1 to preserve its activity and regulate intracellular redox homeostasis

Redox Biology, Journal Year: 2024, Volume and Issue: 70, P. 103080 - 103080

Published: Feb. 8, 2024

Growing evidence suggests that dimethylarginine dimethylaminohydrolase 1 (DDAH1), a crucial enzyme for the degradation of asymmetric (ADMA), is closely related to oxidative stress during development multiple diseases. However, underlying mechanism by which DDAH1 regulates intracellular redox state remains unclear. In present study, was shown interact with peroxiredoxin (PRDX1) and sulfiredoxin (SRXN1), these interactions could be enhanced stress. HepG2 cells, H2O2-induced downregulation accumulation ADMA were attenuated overexpression PRDX1 or SRXN1 but exacerbated knockdown SRXN1. On other hand, also maintained expression in H2O2-treated cells. Furthermore, global knockout Ddah1 (Ddah1-/-) liver-specific (Ddah1HKO) exacerbated, while alleviated liver dysfunction, hepatic CCl4-treated mice. Overexpression improved function, downregulation, diminished differences between wild type Ddah1-/- mice after CCl4 treatment. Collectively, our results suggest regulatory effect on cellular homeostasis under conditions due, at least part, interaction

Language: Английский

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Novel Functional Food Properties of Forest Onion (Eleutherine bulbosa Merr.) Phytochemicals for Treating Metabolic Syndrome: New Insights from a Combined Computational and In Vitro Approach

Nutrients, Journal Year: 2024, Volume and Issue: 16(10), P. 1441 - 1441

Published: May 10, 2024

Metabolic syndrome is a global health problem. The use of functional foods as dietary components has been increasing. One food interest forest onion extract (FOE). This study aimed to investigate the effect FOE on lipid and glucose metabolism in silico vitro using 3T3-L1 mouse cell line. was comprehensive that used multi-modal computational network pharmacology analysis molecular docking cells vitro. phytochemical were analyzed untargeted ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS). Next, an performed determine FOE’s bioactive compounds, toxicity analysis, protein target identification, pharmacology, carried out. pancreatic lipase, α-glucosidase, α-amylase inhibition determined. Finally, we determined its accumulation MAPK8, PPARG, HMGCR, CPT-1, GLP1 expression preadipocyte We showed potential metabolites targeted inhibited lipase levels, Furthermore, significantly (p < 0.05) inhibits expressions GLP-1 dose-dependent manner. contains several reduce α-amylase, proteins associated with

Language: Английский

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Sulfated Polysaccharide from Caulerpa racemosa Attenuates the Obesity-Induced Cardiometabolic Syndrome via Regulating the PRMT1-DDAH-ADMA with mTOR-SIRT1-AMPK Pathways and Gut Microbiota Modulation

Antioxidants, Journal Year: 2023, Volume and Issue: 12(8), P. 1555 - 1555

Published: Aug. 3, 2023

Our investigation intended to analyze the effects of sulfated polysaccharides from

Language: Английский

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Unleashing the Potential of Marine Algae in Cancer Prevention and Treatment Through Combination of Tradition and Innovation

Interdisciplinary cancer research, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Cancer is a global health challenge, with rapidly rising incidence and significant economic burden. The urgent need for innovative approaches to cancer prevention treatment underscored by its growing impact. Marine algae, characterized their unique bioactive compounds, have emerged as promising sources of novel anticancer agents in this context. This chapter reviews the potential marine algae treatment, emphasizing integration traditional knowledge modern scientific methods develop safe, affordable, accessible drugs. offer rich source including polysaccharides, polyphenols, pigments, known antioxidant, anti-inflammatory, antitumor effects. Moreover, these organisms evolved complex defense mechanisms, making them particularly interesting drug discovery. Research highlights multifaceted mechanisms apoptosis induction, cell cycle arrest, selective cytotoxicity against cells. Fucoxanthin, fucoidan, stigmasterol, other compounds from shown potent properties through modulation various signaling pathways. advantages include chemical diversity, sustainability, environmentally friendly cultivation, presenting exciting opportunities development. To address challenges herbal medicine, standardization, active compound identification, rigorous clinical trials are essential. Collaboration between practitioners scientists can bridge gap wisdom medical standards. Combinatorial therapies that integrate extracts conventional treatments more effective holistic management.

Language: Английский

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New insight on antioxidants and anti-obesity properties of two Indonesian seagrass Thalassia hemprichii and Zostera marina: an integrated molecular docking simulation with in vitro study

F1000Research, Journal Year: 2023, Volume and Issue: 12, P. 727 - 727

Published: June 21, 2023

Background: The oceans are teeming with a diverse range of marine organisms that offer unique health benefits, such as seagrass which is one many key products have garnered attention for their potential therapeutic properties. However, until now there been few successful reports seagrass’s metabolites profile and biological activity. Therefore, this work aims to or chemical constituents assess the antioxidants anti-obesity effects two Indonesian seagrasses, Thalassia hemprichii Zostera marina. Methods: Once authenticated, T. Z. marina were extracted different solvents, polar (ethanol) nonpolar (hexane). Metabolite profiling was performed using untargeted metabolomic via liquid chromatography coupled high-resolution tandem mass spectrometry method analysis, then antioxidant capabilities assessed by molecular docking in vitro studies on selected receptors. Results: A total 9 11 observed from continued docking. Some compounds promising inhibitors human inducible nitric oxide synthase, reactive oxygen species (ROS) 1 kinase, pancreatic lipase, fat obesity-associated (FTO) proteins, including luteolin, 6-hydroxy luteolin O-glucoside, luteolin-O-sulphate, Thalassiolin A, C, kaempferol-7,4'-dimethylether-3-O-sulfate, apigenin, diosmetin. ethanol extract (THE) EC50 value shows ABTS radical scavenging activity 76.00 μg/mL, smaller than standard controls (Trolox, 76.54 μg/mL) followed lipase inhibition THE has same pattern (EC50 < Orlistat). Conclusions: This concludes seagrasses candidates functional food and/or drugs in combating free radicals obesity.

Language: Английский

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Revealing Edible Bird Nest as Novel Functional Foods in Combating Metabolic Syndrome: Comprehensive In Silico, In Vitro, and In Vivo Studies

Nutrients, Journal Year: 2023, Volume and Issue: 15(18), P. 3886 - 3886

Published: Sept. 6, 2023

Metabolic dysfunction, which includes intra-abdominal adiposity, glucose intolerance, insulin resistance, dyslipidemia, and hypertension, manifests into metabolic syndrome related diseases. Therefore, the discovery of new therapies in fight against is very challenging. This study aims to reveal existence an edible bird nest (EBN) as a functional food candidate that may be alternative fighting syndrome. The included three approaches: silico molecular docking simulation, vitro, vivo rats fed on cholesterol- fat-enriched diets. Four terpenoids Bakuchiol, Curculigosaponin A, Dehydrolindestrenolide, 1-methyl-3-(1-methyl-ethyl)-benzene EBN have been identified through LCMS/MS-QTOF. In simulations, Bakuchiol Dehydrolindestrenolide are considered potent because they higher inhibitory power four receptors (iNOS, ROS1 kinase, FTO, lipase) than standard drugs. vitro tests also provide insight antioxidant, antidiabetic, antiobesity activities EBN, quite feasible due smaller EC50 value compared Interestingly, studies showed significant improvements (p < 0.05) lipid profile, blood glucose, enzymatic levels, inflammatory biomarkers given high-dose dietary supplementation EBN. More interestingly, upregulates PGC-1α downregulates HMG-CoA reductase. Comprehensively, it has revealed can novel foods for combating

Language: Английский

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Dietary supplementation of Muntingia calabura leaves ameliorates reactive oxygen species and malondialdehyde levels: clinical study on alloxan-induced hyperglycemic rats

Clinical Nutrition Open Science, Journal Year: 2023, Volume and Issue: 48, P. 87 - 96

Published: March 25, 2023

Language: Английский

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Mei-Gin Formula Ameliorates Obesity through Lipolysis, Fatty Oxidation, and Thermogenesis in High-Fat Diet-Induced Obese Rats

Foods, Journal Year: 2023, Volume and Issue: 12(19), P. 3539 - 3539

Published: Sept. 22, 2023

Obesity is a metabolic dysfunction characterized by excessive body fat deposition as consequence of an energy imbalance. Novel therapeutic strategies have emerged that are safe and comparatively low side effects for obesity treatment. Functional foods nutraceuticals recently received great deal attention because their components with the properties antimetabolic syndrome. Based on our previous in vitro vivo investigations anti-adipogenesis activity improved accumulation serials, combination three ingredients (including bainiku-ekisu, black garlic, Mesona procumbens Hemsl), comprising Mei-Gin formula (MGF), was eventually selected novel inhibitor exhibited preventive against obesity. Herein, we verify anti-obesity MGF obese rats induced high-fat diet discuss potential molecular mechanisms underlying development. Oral administration significantly suppressed final weight, weight change, water intake, subcutaneous visceral mass, liver hepatic total lipids triglycerides (TG), serum levels TG, (TC), low-density lipoprotein cholesterol (LDL-C), alanine transaminase (AST), uric acid, ketone bodies augmented fecal lipids, excretion high-dose MGF-supplemented groups. Furthermore, corresponding lipid pathways revealed supplementation effectively increased lipolysis fatty acid oxidation gene expression attenuated synthesis white adipose tissue (WAT) it also mitochondrial activation thermogenic brown (BAT) (HFD). These results demonstrate intake can be beneficial suppression HFD-induced through lipolysis, oxidation, thermogenesis pathway. In conclusion, these efficacy suggest may act agent

Language: Английский

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