Non-Authenticity of Spring Barley Genotypes Revealed in Gene Bank Accessions DOI Creative Commons
Antonín Dreiseitl, Marta Zavřelová

Plants, Journal Year: 2022, Volume and Issue: 11(22), P. 3059 - 3059

Published: Nov. 11, 2022

Plant research and breeding depends on plant genotypes; therefore, genotype authenticity of accessions is the basic requirement for users gene banks. Surprisingly, this extremely important topic rarely reported in scientific community. Non-authentic are that mislabelled undesirable genotypes heterogeneous accessions. In barley, we try to uncover both named problems basis postulated major powdery mildew resistance genes. These diverse, environmentally stable their use well documented suitable characterization. contribution, postulate genes 15 varieties represented by 157 derived lines 32 originating from seven foreign banks compare these findings with previous results including those identically labelled our domestic bank. We found 37.5% bank investigated herein were heterogeneous, at least 20.0% mislabelled. A large-scale molecular characterisation now being carried out, using authentic must be one key requirements. Therefore, each variety a minimum three whose identity has been verified reliable methods should compared before starting new experiments. will involve varietal serve as foundation future science effective crop improvement.

Language: Английский

Powdery Mildew Resistance Genes in European Barley Cultivars Registered in the Czech Republic from 2016 to 2020 DOI Open Access
Antonín Dreiseitl

Genes, Journal Year: 2022, Volume and Issue: 13(7), P. 1274 - 1274

Published: July 18, 2022

Barley is an important crop grown annually on about 55 Mha and intensively cultivated in Europe. In central north-western Europe, spring winter barley can be similar environments which creates suitable conditions for the development of pathogens, including Blumeria graminis f. sp. hordei, causal agent powdery mildew. Apart from pesticide application, it controlled by inexpensive environmentally-friendly genetic resistance. this contribution, results resistance gene identification 58 cultivars to mildew are presented. 56 them their resistances were postulated two hybrid a recently developed method was used. total, 18 known genes found several unknown detected. barley, durable mlo still predominant. MlVe SU Celly only new recorded registered Czech Republic time span. Since 2001 eight specific have been identified country response under field discussed, corresponding responses pathogen population due directional selection. Different strategies breeding recommended.

Language: Английский

Citations

18
When the source of resistance can be considered effective - resistance to Blumeria graminis f. sp. avenae found in Avena sterilis L. DOI Creative Commons
Sylwia Okoń, Aleksandra Nucia, Tomasz Ociepa

et al.

European Journal of Plant Pathology, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

Abstract Powdery mildew, caused by Blumeria graminis DC. f. sp. avenae Em. Marchal, is currently one of the most serious foliar diseases common oats worldwide, leading to significant grain yield losses. So far, 13 resistance genes powdery mildew have been identified and characterized in oats, but only a few these are effective against existing pathogen races. Therefore, there need identify characterize new sources resistance. Avena sterilis L. as promising source fungal diseases. However, identifying requires more extensive research using larger group differentiated isolates B. . This study aimed effectiveness A. genotypes. The focused on 28 genotypes, from IPK Genebank collection, which were known for their high level screening tests. was evaluated host–pathogen tests 55 different collected various regions Europe. These infection patterns genes. In initial stage investigation, previously selected genotypes tested 5 isolates, resulting selection seven resistant Further 50 revealed that two showed very effectiveness. studies indicate should be with variety isolates. Tests based 2–3 suitable preliminary screening.

Language: Английский

Citations

0
Genome-wide association mapping for seedling and adult resistance to powdery mildew in barley DOI Creative Commons
Jie Guo, Chenchen Zhao,

Sanjiv Gupta

et al.

Theoretical and Applied Genetics, Journal Year: 2024, Volume and Issue: 137(3)

Published: Feb. 16, 2024

Abstract Key message Two new major QTL were identified for powdery mildew resistance. We confirmed that the on 7HS contributed mainly to adult-plant resistance, while another one chromosome arm 1HS made a significant contribution seedling Powdery (PM), caused by Blumeria hordei , can occur at all post emergent stages of barley and constantly threatens crop production. To identify more genes effective resistance use in breeding programs, 696 accessions collected from different regions world evaluated PM adult growth three states Australia. These genotyped using DArTSeq with over 18,000 markers genome-wide association study (GWAS). Using FarmCPU model, 54 showed associations scored Another 40 tentative (LOD > 4.0) are distributed across seven chromosomes. Most them grouped into eleven regions, coinciding locations most reported genes. MTAs arms 3HS 5HL, contributing plant 5HL both An MTA

Language: Английский

Citations

2
Discovery of functional NLRs using expression level, high-throughput transformation, and large-scale phenotyping DOI Creative Commons
Helen J. Brabham, Inmaculada Hernández‐Pinzón,

Chizu Yanagihara

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 28, 2024

Abstract Protecting crops from pests and diseases is vital for the sustainable agricultural systems needed food security. Introducing functional resistance genes to enhance plant immune system an effective method of disease control, but identifying new receptors time-consuming resource intensive. We observed that NLR class show a signature high expression in uninfected plants across both monocot dicot species. Here we this signature, combined with throughput crop transformation, can be used rapidly identify candidate NLRs diverse species validate pathogen directly plants. As proof concept, generated wheat transgenic library carrying 995 18 grass Screening collection stem rust Puccinia graminis , which major threat production, confirm 19 genes. This pipeline facilitates gene discovery, unlocking large pool non-domesticated providing in-planta validation crops.

Language: Английский

Citations

2
Identification, High-Density Mapping, and Characterization of New Major Powdery Mildew Resistance Loci From the Emmer Wheat Landrace GZ1 DOI Creative Commons
Zuzana Korchanová, Miroslav Švec, Eva Janáková

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: May 13, 2022

Powdery mildew is one of the most devastating diseases wheat which significantly decreases yield and quality. Identification new sources resistance their implementation in breeding programs effective way disease control. Two major powdery loci conferring to all races seedling adult plant stages were identified emmer landrace GZ1. Their positions, effects, transferability verified using two linkage maps (1,510 codominant SNP markers) constructed from mapping populations (276 lines total) based on resistant GZ1 line. The dominant locus QPm.GZ1-7A was located a 90 cM interval chromosome 7AL explains up 20% trait variation. recessive QPm.GZ1-2A, provides total resistance, 40% variation distal part 2AL. saturated with 14 PCR-based markers delimited 0.99 region corresponds 4.3 Mb cv. Zavitan reference genome comprises 55 predicted genes no apparent candidate for QPm.GZ1-2A gene. No gene or allele at before, suggesting presence data could be used breeding. Moreover, they are an ideal base cloning study host-pathogen interaction pathways determined by genes.

Language: Английский

Citations

5
Rare Virulences and Great Pathotype Diversity of a Central European Blumeria hordei Population DOI Open Access
Antonín Dreiseitl

Published: Sept. 26, 2023

Barley is an important crop grown on almost 49 Mha worldwide in 2021 and particu-larly significant Europe where powdery mildew the most frequent disease sus-ceptible varieties. The suitable way for protecting crops exploiting genetic re-sistance. However, causal agent Blumeria hordei extremely adaptable pathogen. aims of this research were to increase our knowledge rapidly changing path-ogen population detect rare virulences. Random samples pathogen ob-tained from air by means a mobile spore sampler. Spores collected driv-ing across Czech Republic 2019, 2023 299 isolates analyzed 121 host No infection occurred 35 differentials, virulence was recorded 31 varieties higher frequency found 55 differentials. A core set differentials along with four additional distinguish 295 pathotypes (Simple Index = 0.987) complexity varied 4 19 average 10.39. detection new virulences, increasing previously viru-lences high pathotype diversity as well confirm that using nonspecific durable resistance crucial successfully breeding commercial va-rieties.

Language: Английский

Citations

2
Rare Virulences and Great Pathotype Diversity of a Central European Blumeria hordei Population DOI Creative Commons
Antonín Dreiseitl

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(11), P. 1045 - 1045

Published: Oct. 25, 2023

Barley is an important crop grown on almost 49 Mha worldwide in 2021 and particularly significant Europe where powdery mildew the most frequent disease susceptible varieties. The suitable way to protect crops by exploiting genetic resistance. However, causal agent Blumeria hordei extremely adaptable pathogen. aims of this research were increase our knowledge rapidly changing pathogen population detect rare virulences. Random samples obtained from air means a mobile spore sampler. Spores collected driving across Czech Republic 2019, 2023, 299 isolates analyzed 121 host No infection occurred 35 differentials, virulence was recorded 31 varieties higher frequency found 55 differentials. A core set differentials along with four additional distinguishes 295 pathotypes (Simple Index = 0.987) complexity varied 4 19 average 10.39. detection new virulences, increasing previously virulences high pathotype diversity as well confirm that using nonspecific durable resistance crucial for successfully breeding commercial

Language: Английский

Citations

2
Postulation of putative host differentials and pathotyping of Pyricularia setariae (Nishikado) causing leaf blast on foxtail millet [Setaria italica (L.) Beauv] in India DOI

C. H. Sai Bhavana,

K. B. Palanna,

Farooqkhan

et al.

Journal of Plant Pathology, Journal Year: 2024, Volume and Issue: 106(4), P. 1641 - 1653

Published: July 10, 2024

Language: Английский

Citations

0
Powdery Mildew Resistance Genes in Barley Varieties Bred for Human Consumption DOI Creative Commons
Antonín Dreiseitl

Agronomy, Journal Year: 2022, Volume and Issue: 12(10), P. 2245 - 2245

Published: Sept. 20, 2022

Barley has properties that can improve and maintain human health, but to upgrade the positive characteristics of grain, specific breeding programs are required. Consumption chemically protected food is unpopular and, therefore, genetic sources disease resistance most frequent diseases essential. The aim this contribution postulate genes for powdery mildew in spring barley varieties bred consumption. One hundred twenty-seven strains selected from thirty-three crosses, commercial AF Lucius Cesar developed program, eight other check were tested with a set numerous pathogen isolates. Fifteen known found including nonspecific Mlo detected lines 21 crosses. For barley, utilization generally recommended, its importance earmarked consumption should be highlighted because alternative genetically more complicated resistance, derived distant relatives or based on accumulation minor genes, could economically ineffective. presented findings enable fully effective durable selected.

Language: Английский

Citations

1
Non-Authenticity of Spring Barley Genotypes Revealed in Gene Bank Accessions DOI Creative Commons
Antonín Dreiseitl, Marta Zavřelová

Plants, Journal Year: 2022, Volume and Issue: 11(22), P. 3059 - 3059

Published: Nov. 11, 2022

Plant research and breeding depends on plant genotypes; therefore, genotype authenticity of accessions is the basic requirement for users gene banks. Surprisingly, this extremely important topic rarely reported in scientific community. Non-authentic are that mislabelled undesirable genotypes heterogeneous accessions. In barley, we try to uncover both named problems basis postulated major powdery mildew resistance genes. These diverse, environmentally stable their use well documented suitable characterization. contribution, postulate genes 15 varieties represented by 157 derived lines 32 originating from seven foreign banks compare these findings with previous results including those identically labelled our domestic bank. We found 37.5% bank investigated herein were heterogeneous, at least 20.0% mislabelled. A large-scale molecular characterisation now being carried out, using authentic must be one key requirements. Therefore, each variety a minimum three whose identity has been verified reliable methods should compared before starting new experiments. will involve varietal serve as foundation future science effective crop improvement.

Language: Английский

Citations

1