Postbiotic studies of mixed cultures of Schleiferilactobacillus harbinensis LH991 and Pichia kudriavzevii B-5P produced by in vitro rumen producing short-chain fatty acid DOI Creative Commons
Yetti Marlida, Joo Shun Tan, Syofyan Syofyan

et al.

Veterinary World, Journal Year: 2024, Volume and Issue: unknown, P. 2694 - 2700

Published: Nov. 1, 2024

Postbiotics are functional bioactive compounds or molecules with beneficial effects on health and activities in humans livestock, produced by probiotic bacteria yeast. Several postbiotics, including enzymes, short-chain fatty acids, amino extracellular polysaccharides, microbial cell fragments, teichoic currently being widely studied. This study aimed to explore the potential of secondary metabolites Schleiferilactobacillus harbinensis LH 991 Pichia kudriavzevii B-5P as lactic acid (LAB) yeast isolated from Budu (fermented fish) which can act postbiotics through vitro rumen fermentation. The method used a completely randomized design 5 × 4, five treatments four replications. substrate diet consisted 60% forage 40% concentrate. culture mixture was 1.3 1011 CFU/mL 50%:50% ratio S. P. B-5P. inoculum concentrations this were 0% (control), 1%, 2%, 3%, 4%. Treatments arranged based differences concentration follows: T0: control (0%); T1: 1%; T2: 2%; T3: 3%; T4: T4 group showed significant increase (p < 0.01) acids (SCFA), acetate, propionate, butyrate, valerate, isobutyrate, isovalerate compared other treatments. Meanwhile, shows that there is no > effect digestibility (in dry matter digestibility, organic crude fiber digestibility). However, highly volatile total, NH3, protein synthesis. It concluded treatment 4% (T4) containing LAB increased SCFA fermentation significantly, whereas it did not affect digestibility.

Language: Английский

Probiotics and Paraprobiotics: Effects on Microbiota-Gut-Brain Axis and Their Consequent Potential in Neuropsychiatric Therapy DOI Creative Commons
Samriti Balaji Mudaliar, Sumith Sundara Poojary,

Alevoor Srinivas Bharath Prasad

et al.

Probiotics and Antimicrobial Proteins, Journal Year: 2024, Volume and Issue: 16(4), P. 1440 - 1464

Published: Jan. 31, 2024

Abstract Neuropsychiatric disorders are clinical conditions that affect cognitive function and emotional stability, often resulting from damage or disease in the central nervous system (CNS). These a worldwide concern, impacting approximately 12.5% of global population. The gut microbiota has been linked to neurological development function, implicating its involvement neuropsychiatric conditions. Due their interaction with microbial communities, probiotics offer natural alternative traditional treatments such as therapeutic drugs interventions for alleviating symptoms. Introduced by Metchnikoff early 1900s, live microorganisms provide various health benefits, including improved digestion, enhanced sleep quality, reduced mental problems. However, concerns about safety, particularly immunocompromised patients, warrant further investigation; this led concept “paraprobiotics”, inactivated forms beneficial safer alternative. This review begins exploring different methods inactivation, each targeting specific cellular components like DNA proteins. choice inactivation method is crucial, benefits may vary depending on employed inactivation. subsequent sections focus potential mechanisms action applications paraprobiotics therapy. Probiotics interact microbes, modulating composition dysbiosis. primarily stem gut-brain axis, bidirectional communication channel involving pathways discussed review. While research needed, promising agents management disorders.

Language: Английский

Citations

13
Antibiotic influx and efflux in Pseudomonas aeruginosa: Regulation and therapeutic implications DOI Creative Commons

Weiyan Wu,

Jiahui Huang,

Zeling Xu

et al.

Microbial Biotechnology, Journal Year: 2024, Volume and Issue: 17(5)

Published: May 1, 2024

Abstract Pseudomonas aeruginosa is a notorious multidrug‐resistant pathogen that poses serious and growing threat to the worldwide public health. The expression of resistance determinants exquisitely modulated by abundant regulatory proteins intricate signal sensing transduction systems in this pathogen. Downregulation antibiotic influx porin upregulation efflux pump owing mutational changes their regulators or presence distinct inducing molecular signals represent two most efficient mechanisms restrict intracellular accumulation enable P. resist multiple antibiotics. Treatment infections extremely challenging due highly inducible mechanism resistance. This review comprehensively summarizes networks major (OprD OprH) pumps (MexAB‐OprM, MexCD‐OprJ, MexEF‐OprN, MexXY) play critical roles . It also discusses promising therapeutic approaches using safe adjuvants enhance efficacy conventional antibiotics combat controlling levels porins pumps. not only highlights complexity network induces but provides important implications targeting

Language: Английский

Citations

8
Characterization and Assessment of Native Lactic Acid Bacteria from Broiler Intestines for Potential Probiotic Properties DOI Creative Commons
Xiaoxia Li, Li Wang,

Longmei Zhao

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(4), P. 749 - 749

Published: April 7, 2024

Probiotics are the most promising alternative to antibiotics for improving animal production and controlling pathogenic infections, while strains derived from natural hosts considered highly desirable due their good adaptation gastrointestinal tract. The aim of this study was screen Lactobacillus with broad-spectrum antibacterial activity broilers fed an antibiotic-free diet evaluate potential as poultry probiotics. A total 44 lactic acid bacteria (LAB) were isolated intestines healthy broilers, among which 3 exhibited outstanding antimicrobial subsequently identified through 16S rRNA sequencing Enterococcus faecium L8, Lactiplantibacillus plantarum L10, Limosilactobacillus reuteri H11. These three isolates demonstrated potent bacteriostatic against Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, Salmonella cholerae, inhibition zones ranging 15.67 ± 1.53 21.33 0.58 mm. selected LAB high tolerance bile salts, L. H11 displaying highest survival rate (ranging 34.68% 110.28%) after exposure 0.3% (w/v) salts 6 h or a low pH environment (pH 2, 2.5, 3) h. Notably, outperformed other in terms hydrophobicity (84.31%), auto-aggregation (53.12%), co−aggregation E. coli ATCC 25922 (36.81%) S. aureus 6538 (40.20%). In addition, either fully moderately susceptible tested antibiotics, except strain resisted gentamycin vancomycin. Consequently, these strains, especially H11, broiler chickens, represent probiotic candidates that can be employed feed additives enhance performance control pathogens.

Language: Английский

Citations

5
Effects of Dietary Supplementation with Lactobacillus reuteri Postbiotics on Growth Performance, Intestinal Flora Structure and Plasma Metabolome of Weaned Piglets DOI Creative Commons

Dongfeng Sun,

Wenfei Tong,

Sanghoon Han

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(2), P. 204 - 204

Published: Jan. 14, 2025

Probiotics and their postbiotics have the potential to improve health growth performance of piglets, which has brought them widespread attention in post-antibiotic era. In present study, effects dietary supplementation Lactobacillus reuteri on performance, intestinal flora structure plasma metabolome weaned piglets were investigated. A total 816 healthy male with uniform weight divided into two treatment groups: control (CTR) group fed a basic diet, ones LAC diet supplemented 500 mg/kg postbiotics. There six replicates each 68 replicate. The animal trial lasted for 30 days. feces blood collected investigation, during was counted. Our outcomes show that had no effect piglets; however, it reduced mortality rate by 6.37%. levels superoxide dismutase serum, propionic acid butyric elevated, content malondialdehyde serum decreased postbiotics-treated (p < 0.05). fecal sequencing results relative abundance Firmicutes monoglobus upregulated, Bacteroides downregulated addition, positively correlated negatively raised level coenzyme Q10 serum. conclusion, contributed improving antioxidant function reducing regulating upregulating

Language: Английский

Citations

0
Effects of maternal-offspring supplementation of probiotics and synbiotics on the immunity of offspring Bama mini-pigs DOI Creative Commons
Sujuan Ding, Ting Ye, Md. Abul Kalam Azad

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 13, 2025

Maternal nutrition is one of the main factors regulating growth and immunity piglets. This study aimed to investigate effects maternal or maternal-offspring supplementation antibiotics, probiotics, synbiotics on offspring (21, 65, 125 day-old) in Bama mini-pigs. The results showed that adding antibiotics diets increased plasma IFN-γ level piglets at 21 day-old. Compared with supplementation, decreased IL-10 spleen, probiotics IL-2, IL-10, TNF-α levels ileum, ileum Moreover, IL-1β while spleen 95 day-old compared supplementation. Maternal-offspring IL-6 than pigs, which was higher These findings suggest immune function varied depending specific approach used for

Language: Английский

Citations

0
Parabacteroides goldsteinii Alleviates Intestinal Inflammation in Dextran Sulfate Sodium-Treated Pigs DOI Creative Commons

Deng Xu,

Tao Guo,

Yang He

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(9), P. 1231 - 1231

Published: April 27, 2025

Intestinal inflammation in piglets leads to diarrhea, decreased immune function, and dysbiosis of the gut microbiota. Probiotics are widely recognized used prevention control enteritis piglets. Parabacteroides goldsteinii (PG) is a probiotic, there few reports on anti-inflammatory properties this Therefore, study selected 10 Duroc × Landrace Yorkshire (DLY) aged 50 days randomly divided them into group (CT group) an experimental (PG for 14-day experiment. During 1-7, PG established model by gavage with 4% dextran sulfate sodium (DSS). The results showed that DSS increased content IL-6 IL-8 (p < 0.05), while it IL-10 serum For 8-14, DSS-treated were administered 7.9 108 CFU/mL suspension via gavage. IL-6, IL-8, did not differ between CT groups > 0.05). Some beneficial bacteria, such as Lactobacillales Butyricimonas, significantly enriched lower alpha diversity metabolic pathways exhibited highest abundance KEGG functional prediction analysis. above studies confirm alleviates intestinal changes microbial composition.

Language: Английский

Citations

0
Development of postbiotic-enriched chitosan films as a novel approach for sustainable food packaging DOI
Mohamed Ghamry,

Fatma Ibrahim,

Qais Ali Al‐Maqtari

et al.

Food Hydrocolloids, Journal Year: 2025, Volume and Issue: unknown, P. 111515 - 111515

Published: May 1, 2025

Language: Английский

Citations

0
Metabolite profiling and bioactivity guided fractionation of Lactobacillaceae and rice bran postbiotics for antimicrobial-resistant Salmonella Typhimurium growth suppression DOI Creative Commons
Nora Jean Nealon, Colette R. Worcester, Shea M. Boyer

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 9, 2024

Probiotic-fermented supplements (postbiotics) are becoming increasingly explored for their activity against antibiotic-resistant enteropathogens. Prebiotics often incorporated into postbiotics to enhance efficacy, but due strain differences in probiotic activity, postbiotic antimicrobial effects poorly understood. To improve we investigated and compared metabolite profiles of prepared with three lactic acid bacteria strains (

Language: Английский

Citations

3
The Effects of Dietary Saccharomyces cerevisiae Supplementation on Gut Microbiota Composition and Gut Health in Aged Labrador Retrievers DOI Creative Commons
Yingyue Cui,

Deping Li,

Mingrui Zhang

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(12), P. 1713 - 1713

Published: June 7, 2024

The intestinal microbiome changes with age, influencing the host’s health and immune status. Saccharomyces cerevisiae (S. cerevisiae) positively affects function in humans animals, but its effects on gut microbiota profile aged dogs have not been thoroughly investigated. Twenty Labrador Retrievers were divided into two groups: a control group (CON) S. (SC). experiment lasted for 42 days, assessments of their barrier function, inflammatory factors, antioxidant markers, fecal composition. results showed that dietary reduced levels TNF-α, IL-6, IL-1β serum (p < 0.05). In SC group, plasma superoxide dismutase glutathione peroxidase activities increased, while level malondialdehyde significantly decreased Additionally, lowered zonulin lipopolysaccharide (LPS) 0.05) inhibited ammonia production Furthermore, abundance Firmicutes increased Chao index, Bacteroidetes, proportion Bacteroidetes to To conclude, can regulate gut’s microbial structure health, which may contribute overall companion animals as they age.

Language: Английский

Citations

2
Bacillus spp. as potential probiotics: promoting piglet growth by improving intestinal health DOI Creative Commons
Xiaopeng Tang, Yan Zeng, Kangning Xiong

et al.

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: July 26, 2024

The application of

Language: Английский

Citations

2