Soybean GmWRKY44 transcription factor activates SOC1 and LFY to promote flowering in Arabidopsis thaliana DOI
Yanchong Yu, Youju Huang,

Cuijing Pang

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract WRKY transcription factors (TFs) play pivotal roles in regulating plant flowering; however, the molecular mechanism underlying flowering regulation by soybean (Glycine max) TFs remains elusive. In this study, we isolated GmWRKY44, a nuclear-localized Group IIc member exhibiting transcriptional activation capacity. GmWRKY44 displayed spatiotemporal specificity, with peak expression 30 d post-germination stems. GUS staining showed that expressed various tissues, such as roots, stems, leaves, sepals, stigmas, filaments, siliques and seedlings. promoter harbored 92 cis-elements associated phytohormone responses, light signaling, abiotic stress adaptation developmental regulation. Furthermore, overexpression of Arabidopsis led to an early-flowering phenotype, evidenced significant upregulation activators SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), LEAFY (LFY), APETALA1 (AP1) downregulation repressor FLOWERING LOCUS C (FLC). Subsequent analyses, including Y1H, EMSA, LUC assays, provided convincing evidences GmWRKY44 directly bound promoters SOC1 LFY, thereby elevating their expression. Genetic complementation assays further revealed OE44-1 soc1-2 lfy-2 hybrids exhibited later time than OE44-1 plants, indicating loss or LFY genetically arrested OE44-1. summary, study promoted upregulating thus addressing critical knowledge gap WRKYs on offering novel candidate gene for optimizing enhance yield across diverse agroecological zones.

Language: Английский

WRKY transcription factors: Hubs for regulating plant growth and stress responses DOI Open Access
Lu Yang, Siyu Fang, Lei Liu

et al.

Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

As sessile organisms, plants must directly face various stressors. Therefore, have evolved a powerful stress resistance system and can adjust their growth development strategies appropriately in different stressful environments to adapt complex ever-changing conditions. Nevertheless, prioritizing defensive responses hinder growth; this is crucial factor for plant survival but detrimental crop production. such, comprehending the impact of adverse on not only fundamental scientific inquiry also imperative agricultural industry food security. The traditional view that hindered during defense due resource allocation trade-offs challenged by evidence exhibit both robust capabilities through human intervention. These findings suggest growth‒defense trade-off dictated limitations influenced intricate transcriptional regulatory mechanisms. Hence, it conduct thorough investigations central genes govern unfavorable environments. Recent studies consistently highlighted importance WRKY transcription factors orchestrating plant-specific development, underscoring pivotal role WRKYs modulating under Here, we review recent advances understanding dual roles regulation across diverse This information will be elucidating interplay between response may aid identifying gene loci could utilized future breeding programs develop crops with enhanced productivity.

Language: Английский

Citations

3
Unlocking nature's secrets: The pivotal role of WRKY transcription factors in plant flowering and fruit development DOI
Shazia Rehman,

Saraj Bahadur,

Wei Xia

et al.

Plant Science, Journal Year: 2024, Volume and Issue: 346, P. 112150 - 112150

Published: June 11, 2024

Language: Английский

Citations

4
WRKY Transcription Factors (TFs) as Key Regulators of Plant Resilience to Environmental Stresses: Current Perspective DOI Creative Commons
Hui Li, Muneer Ahmed Khoso, Xu He

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(10), P. 2421 - 2421

Published: Oct. 19, 2024

Plants encounter various stresses in their natural environments and can effectively respond to only one stress at a time. Through complex gene network, transcription factors (TFs) such as WRKY TFs regulate diverse array of responses. The clarification the structural characteristics proteins, along with recent advancements molecular dynamics simulations, has shed light on formation, stability, interactions DNA–protein complexes. This provided novel viewpoint regarding control TFs. investigation superfamilies, encompassing historical development, diversity, evolutionary patterns, become feasible due transcriptome approach’s capacity provide extensive comprehensive transcripts. significance lies pivotal role within several signaling cascades regulatory networks that influence plant defense present review summarizes functional aspects high-volume sequence data from different species studied date. Moreover, comparative analysis approach was utilized determine functions identified response both abiotic biotic stresses, revealed through numerous studies species. results this will be understanding events context climate change, incorporating new scientific evidence propose an innovative viewpoint.

Language: Английский

Citations

4
Salt stimulates carbon fixation in the halophyte <i>Nitraria sibirica</i> to enhance growth DOI Creative Commons
Lu Lu, Yu-Ru Wang, Yu‐Chang Chen

et al.

Forestry Research, Journal Year: 2025, Volume and Issue: 5(1), P. 0 - 0

Published: Jan. 1, 2025

Soil salinity significantly inhibits plant productivity by adversely affecting photosynthesis and growth. Nitraria sibirica, a typical halophyte, exhibits strong salt tolerance. In this study, salt-treated sibirica seedlings demonstrated more vigorous growth higher photosynthetic rate than untreated control seedlings. Transcriptome analysis revealed that the upregulated differentially expressed genes including ribose 5-phosphate isomerase A, ribulose-bisphosphate carboxylase large chain, malate dehydrogenase in leaves of treated with 500 mM NaCl were enriched 'Carbon fixation organisms' pathway according to Kyoto Encyclopedia Genes Genomes database. The promoters these three predicted contain cis-regulatory elements responsive light, abscisic acid, ethylene. Notably, encoding 1-aminocyclopropane-1-carboxylate synthase, key enzyme ethylene biosynthesis, ethylene-responsive transcription factors under treatment. Furthermore, quantitative real-time PCR confirmed expression was ethephon for 1 h. contrast, salt-induced downregulated μM aminoethoxyvinylglycine, an biosynthesis inhibitor, combination These findings suggest enhanced observed stress is likely mediated signaling, which regulates involved carbon fixation, thereby promoting

Language: Английский

Citations

0
Soybean GmWRKY44 transcription factor activates SOC1 and LFY to promote flowering in Arabidopsis thaliana DOI
Yanchong Yu, Youju Huang,

Cuijing Pang

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract WRKY transcription factors (TFs) play pivotal roles in regulating plant flowering; however, the molecular mechanism underlying flowering regulation by soybean (Glycine max) TFs remains elusive. In this study, we isolated GmWRKY44, a nuclear-localized Group IIc member exhibiting transcriptional activation capacity. GmWRKY44 displayed spatiotemporal specificity, with peak expression 30 d post-germination stems. GUS staining showed that expressed various tissues, such as roots, stems, leaves, sepals, stigmas, filaments, siliques and seedlings. promoter harbored 92 cis-elements associated phytohormone responses, light signaling, abiotic stress adaptation developmental regulation. Furthermore, overexpression of Arabidopsis led to an early-flowering phenotype, evidenced significant upregulation activators SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), LEAFY (LFY), APETALA1 (AP1) downregulation repressor FLOWERING LOCUS C (FLC). Subsequent analyses, including Y1H, EMSA, LUC assays, provided convincing evidences GmWRKY44 directly bound promoters SOC1 LFY, thereby elevating their expression. Genetic complementation assays further revealed OE44-1 soc1-2 lfy-2 hybrids exhibited later time than OE44-1 plants, indicating loss or LFY genetically arrested OE44-1. summary, study promoted upregulating thus addressing critical knowledge gap WRKYs on offering novel candidate gene for optimizing enhance yield across diverse agroecological zones.

Language: Английский

Citations

0