Mitigating Photoaged Skin Aging with Single Injection of Recombinant Human Collagen Type Iii DOI
Yafang Chen, Kang Li, Hu Chen

et al.

Published: Jan. 1, 2023

Delaying aging is an eternal goal for humanity. Collagen-based materials, employed as dermal fillers to smoothen wrinkles, have attained extensive utilization. Nevertheless, traditional animal-derived collagen protein primarily presents concerns pertaining disease risks, potential immunological reactions, and batch instability. In this investigation, the filler composed of recombinant human type III (rhCol III) with excellent injectability was fabricated using synthetic biology. The in vitro examination cell interaction rhCol revealed commendable biocompatibility, concentration-dependent impacts on proliferation, migration secretion. Subsequently, we established a skin damage animal model through UV-photoaging introduced bioactive material vivo implantation, systematically comparing its biological effect pCollagen, hyaluronic acid (HA) saline thorough histological observation, immunofluorescence staining, hydroxyproline quantification analysis specific gene expression. Outcomes indicated prompted augmented production I (Col I), elastic fibers, thereby contributing remodeling extracellular matrix (ECM). summary, our investigation contributed robust biosafety proficient attenuation UV-induced photoaging by under single injection. Despite imperative ongoing efforts required successful translation from bench clinic, discernibly superior safety efficacy profile positioned it promising candidate within domain fillers.

Language: Английский

Bio-Based Composite Membranes from Fish Scales: A Novel Approach to Harnessing Collagen and Hydroxyapatite for Tissue Engineering Applications DOI Creative Commons
Israel Núñez-Tapia,

Jimena Macouzet-Garduño,

Fernanda Ramírez-Ruiz

et al.

Biomedical Engineering Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100146 - 100146

Published: Feb. 1, 2025

Language: Английский

Citations

2
Collagen-Based Wound Dressings: Innovations, Mechanisms, and Clinical Applications DOI Creative Commons

A. S. Alberts,

Ana‐Maria Bratu, Adelina-Gabriela Niculescu

et al.

Gels, Journal Year: 2025, Volume and Issue: 11(4), P. 271 - 271

Published: April 5, 2025

Collagen-based wound dressings have developed as an essential component of contemporary care, utilizing collagen’s inherent properties to promote healing. This review thoroughly analyzes collagen dressing advances, examining different formulations such hydrogels, films, and foams that enhance care. The important processes by which promotes healing (e.g., promoting angiogenesis, encouraging cell proliferation, offering structural support) are discussed clarify its function in tissue regeneration. effectiveness adaptability demonstrated via clinical applications investigated acute chronic wounds. Additionally, commercially accessible collagen-based skin treatments discussed, demonstrating their practical use healthcare settings. Despite the progress, study discusses obstacles restrictions encountered producing adopting dressings, difficulties manufacturing financial concerns. Finally, current landscape’s insights indicate future research possibilities for optimization, bioactive agent integration, overcoming existing constraints. analysis highlights potential innovations improve treatment methods patient

Language: Английский

Citations

1
Fish and bovine collagen promote higher migration and adhesion of dermal cells pre-treated with wound-healing herbal extracts DOI
M. Ben Rejeb,

Aida Lahmar,

Mohamed Bayrem Ghedira

et al.

Tissue and Cell, Journal Year: 2025, Volume and Issue: 93, P. 102762 - 102762

Published: Jan. 30, 2025

Language: Английский

Citations

0
From Bench to Clinic: Crosslinking Approaches for Next‐Generation Collagen Fillers DOI Creative Commons

Song-Yi Wu,

Chen-Chi Tsai,

Wei‐Bor Tsai

et al.

Advances in Polymer Technology, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

With a deeper understanding of the aging process, injectable dermal fillers have revolutionized cosmetic dermatology and plastic surgery. These minimally invasive treatments address signs aging, such as wrinkles, fine lines, volume loss. The market for expands yearly, with each product offering unique compositions that influence therapeutic outcomes, handling properties, potential adverse effects. Fillers are generally classified into three major types: temporary, semi‐permanent, permanent. Temporary fillers, including hyaluronic acid (HA) collagen (COL)‐based options, provide reliable correction but typically limited longevity. Semi‐permanent permanent made from synthetic materials like poly‐L‐lactic polymethylmethacrylate (PMMA), offer extended durations neocollagenesis. This review focuses specifically on COL‐based discussing both FDA‐approved products those still in research stage.

Language: Английский

Citations

0
Injection of Different Concentrations of Recombinant Humanized Type III Collagen Into the Dermis Promotes Type III and Type I Collagen Deposition as Well as Angiogenesis: An In Vivo Study DOI
Haibo Xiang, Yimou Sun, Yujie Chen

et al.

Dermatologic Surgery, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Type III recombinant humanized collagen (rhCol III) has a promising future in cosmetic applications. To investigate the histological changes associated with injection of different concentrations rhCol into dermis. Twenty mice were divided control, low-dose (LD) group, and high-dose (HD) group. The animals euthanized 1, 2, 3, 4 weeks after injection. Injections induced an increase formation concentration-dependent manner. I (Col I) was upregulated both HD LD groups to varying extents, often similar that Col III. Fibroblast proliferation plays important role deposition promoted angiogenesis at later stages, regardless concentration dependency. Intradermal injections can promote fibroblast proliferation, enhancing III, stimulating angiogenesis. Further studies are required elucidate mechanisms.

Language: Английский

Citations

0
Extracellular Matrix Stiffness: Mechanotransduction and Mechanobiological Response-Driven Strategies for Biomedical Applications Targeting Fibroblast Inflammation DOI Open Access
Watcharaphol Tiskratok, Nontawat Chuinsiri, Phoonsuk Limraksasin

et al.

Polymers, Journal Year: 2025, Volume and Issue: 17(6), P. 822 - 822

Published: March 20, 2025

The extracellular matrix (ECM) is a dynamic network providing mechanical and biochemical cues that regulate cellular behavior. ECM stiffness critically influences fibroblasts, the primary producers, particularly in inflammation fibrosis. This review explores role of fibroblast-driven tissue remodeling, focusing on physicochemical biological mechanisms involved. Engineered materials, hydrogels, polydimethylsiloxane (PDMS) are highlighted for replicating tissue-specific stiffness, enabling precise control over cell–matrix interactions. surface functionalization substrate including collagen, polydopamine, fibronectin, enhances bioactivity fibroblast adhesion. Key mechanotransduction pathways, such as integrin signaling YAP/TAZ activation, related to regulating behaviors inflammatory responses. fibroblasts driving chronic diseases emphasizes their therapeutic potentials. Advances ECM-modifying strategies, tunable biomaterials hydrogel-based therapies, explored applications engineering, drug delivery, anti-inflammatory treatments, diagnostic tools accurate diagnosis prognosis stiffness-related diseases. integrates mechanobiology with biomedical innovations, comprehensive responses outlining future directions targeted therapies.

Language: Английский

Citations

0
Collagen-based micro/nanogel delivery systems: Manufacturing, release mechanisms, and biomedical applications DOI Creative Commons

Bowei Du,

Shanhui Feng, J. Wang

et al.

Chinese Medical Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Abstract Collagen-based materials, renowned for their biocompatibility and minimal immunogenicity, serve as exemplary substrates in a myriad of biomedical applications. micro/nanogels, particular, are valued increased surface area, tunable degradation rates, ability to facilitate targeted drug delivery, making them instrumental advanced therapeutics tissue engineering endeavors. Although extensive reviews on micro/nanogels exist, they tend cover wide range biomaterials lack specific focus collagen-based materials. The current review offers an in-depth look into the manufacturing technologies, release mechanisms, applications address this gap. First, we provide overview synthetic strategies that allow precise control size, shape, mechanical strength these by controlling degree cross-linking These properties crucial performance We then highlight environmental responsiveness particularly sensitivity enzymes pH, which enables controlled under various pathological conditions. discussion expands include cancer therapy, antimicrobial treatments, bone repair, imaging diagnosis, emphasizing versatility potential critical areas. challenges future perspectives field discussed at end review, with emphasis translation clinical practice. This comprehensive serves valuable resource researchers, clinicians, scientists alike, providing insights state directions micro/nanogel research development.

Language: Английский

Citations

0
Collagen-Based Medical Devices for Regenerative Medicine and Tissue Engineering DOI
Mehdi Abedi,

Mina Shafiee,

Farideh Afshari

et al.

Applied Biochemistry and Biotechnology, Journal Year: 2023, Volume and Issue: 196(8), P. 5563 - 5603

Published: Dec. 22, 2023

Language: Английский

Citations

9
Perturbed collagen metabolism underlies lymphatic recanalization failure in Gata2 heterozygous deficient mice DOI
Tomomi Watanabe‐Asaka, Moyuru Hayashi, Takuya Harada

et al.

The Journal of Biochemistry, Journal Year: 2024, Volume and Issue: 175(5), P. 551 - 560

Published: Jan. 2, 2024

Abstract Lymphedema has become a global health issue following the growing number of cancer surgeries. Curative or supportive therapeutics have long been awaited for this refractory condition. Transcription factor GATA2 is crucial in lymphatic development and maintenance, as haploinsufficient disease often manifests lymphedema. We recently demonstrated that Gata2 heterozygous deficient mice displayed delayed recanalization upon lymph node resection. However, whether contributes to regeneration by functioning damaged vessels' microenvironment remains explored. In study, our integrated analysis dermal collagen fibers were more densely accumulated mice. The metabolism-related transcriptome was perturbed, matrix contractile activity aberrantly increased embryonic fibroblasts. Notably, soluble placement ameliorated recanalization, presumably modulating stiffness extracellular around resection site Our results provide valuable insights into mechanisms underlying GATA2-haploinsufficiency-mediated lymphedema shed light on potential therapeutic avenues intractable disease.

Language: Английский

Citations

3
New Regenerative and Anti-Aging Medicine Approach Based on Single-Stranded Alpha-1 Collagen for Neo-Collagenesis Induction: Clinical and Instrumental Experience of a New Injective Polycomponent Formulation for Dermal Regeneration DOI Creative Commons
Luigi Di Rosa,

Antonino De Pasquale,

Sara Baldassano

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(4), P. 916 - 916

Published: April 20, 2024

This study explores the efficacy of a novel polycomponent formulation (KARISMA Rh Collagen® FACE, Taumedika Srl, Rome, Italy), containing 200 mg/mL non-crosslinked high-molecular-weight hyaluronic acid (HMW-HA), μg/mL human recombinant polypeptide collagen-1 alpha chain, and 40 carboxymethyl cellulose (CMC) as regenerative medicine for skin regeneration rejuvenation. combines acid, to stimulate collagen type I production enhance hydration. involved 100 subjects with varying conditions, divided into three groups based on aging, smoking history, facial scarring, evaluate product’s effectiveness in aesthetic improvement. The methodology included two injections Karisma (2 mL each injection) one month apart, evaluations conducted using FACE-Q questionnaires, SGAIS Questionnaire, Antera 3D scanner measurements at baseline, 30 days, 60 days post-treatment. results demonstrated significant reduction roughness an improvement quality across all groups, no correlation between outcomes patient’s age. subjective assessments also indicated high satisfaction treatment’s results. analyzed data allow us conclude that single-stranded carboxymethyl-cellulose is able skin’s response, yielding both vitro and, through our study, vivo. new effectively stimulates regeneration, improving texture, benefits perceived by patients, low incidence adverse events, marking promising advancement medicine.

Language: Английский

Citations

3