Influence of bacterial infection on human sperm DOI Creative Commons

Yousry Khalaf Sadeek,

Tarek M. Abdelghany,

Aii Gamal Gadel-Rab

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 13, 2024

Abstract Background Invasion of bacteria in to the male reproductive system associated with decrease sperm function, leading impair fertilizing ability, human body harbors trillions microbes, and their influence on health has been explored many parts body, there is a general agreement negative impact some pathogenic bacterial species semen parameters, including counts, motility, morphology, Deoxyribonucleic acid (DNA) integrity. The Aim this study was evaluate impacts microbial infection parameters functions. This work included 186 specimen from sub fertile men unselected couples consulting infertility clinic center. All samples examined bacteriologically, also quality, evaluated according world Health organization guideline (WHO,2010). Results patients investigated for infertility, 65 (34.94%) studied specimens were infected different species. strains identified as Enterococcus Fecails, staphylococcus haemolyticyus, Escherichia coli, Micrococcus lylae Serratia Marcescens. Infected had quality (count, progressive, vitality normality. Moreover, high Reactive oxygen (ROS) function which include Acrosin activity (A.A) Hypo-osmotic swelling test (HOST) noticed comparison non-infected but non-significant. Conclusion lead ability sperm.

Language: Английский

Antibiotics in semen extenders – a multiplicity of paradoxes DOI
Jane M. Morrell, Aleksandar Cojkić, Pongpreecha Malaluang

et al.

Reproduction Fertility and Development, Journal Year: 2024, Volume and Issue: 36(5)

Published: March 6, 2024

Addition of antibiotics to semen extenders was taken for granted many years, from the time that commercial artificial insemination in livestock first began decades ago. However, there is now a growing realisation this non-therapeutic utilisation antibacterial agents contrary current recommendations prudent use medical and veterinary professionals are advised follow. Furthermore, not benign, having negative effects on sperm samples, inseminated female, personnel potentially environment. The purpose review three-fold: highlight fact used extenders, with result considerable amounts globally animal breeding, recent studies aspects using purpose, look at possible alternatives. Recent changes legislation regarding occurred some, but all, countries, leaving question marks producers as whether should be added or not.

Language: Английский

Citations

8
Innovative Approaches to Avoid Antibiotic Use in Equine Semen Cryopreservation: Advancing Sustainable Reproductive Technologies DOI Creative Commons
S. Zabala, Consuelo Serres, Natalia Montero

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(10), P. 1368 - 1368

Published: May 9, 2025

This study evaluated the impact of different processing techniques on microbial load and sperm quality in frozen–thawed equine semen to identify alternatives reduce preventive use antibiotics. Semen was obtained processed under rigorous hygiene measures from ten stallions, using four protocols: Simple Centrifugation with antibiotics (S+) (S−), Filtration (F−) Single-Layer Colloidal (C−) an antibiotic-free extender. Microbial culture media, viability motility were assessed. results consistent across protocols, except Columbia 5% Sheep Blood Agar where S− exhibited higher than S+ (p < 0.05). However, F− C− showed similar loads S+. No significant differences observed progressive motility, average path velocity, straight-line velocity or wobble parameters between protocols. Total significantly compared other treatments Thus, regardless antibiotics, proposed methods achieved traditional antibiotic-inclusive protocol terms most relevant parameters. These findings suggest that C−, combined optimized measures, offers effective alternative prophylactic extenders.

Language: Английский

Citations

0
Boar semen storage at 5 °C for the reduction of antibiotic use in pig insemination: Pathways from science into practice DOI Creative Commons
Dagmar Waberski, Anne‐Marie Luther

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 269, P. 107486 - 107486

Published: April 30, 2024

Storage of boar semen at 5 °C instead the conventional temperature 17 is an innovative preservation concept. It enhances protection against growth bacteria normally occurring in ejaculates and potential drug-resistant contaminants from environment. Thereby it allows reduction or even elimination antibiotics porcine extenders. The present article reviews current state low-temperature approach semen, with a special focus on antimicrobial efficiency fertility field insemination trials. Particularly role extenders management for achievement high biosecurity are elucidated. Insemination data 1,841 sows there different countries revealed equally farrowing rates litter sizes stored compared to controls °C. Microbiology obtained doses spiked multi-drug resistant showed cold storage inhibiting Serratia marcescens, bacterial species sperm-toxicity. Evolving concepts physiological male reproductive microbiome female provides further argument complete eradication dose by antibiotic additives Finally, motivation practical considerations use novel tool artificial pigs revealed, which might encourage transformation towards sustainable production following One Health approach.

Language: Английский

Citations

2
Fertility after photodynamic inactivation of bacteria in extended boar semen DOI Creative Commons
Anne‐Marie Luther, Mohammad Varzandeh,

Christina Beckermann

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 7, 2024

Antimicrobial resistance is an increasing challenge in semen preservation of breeding animals, especially the porcine species. Bacteria are a natural component semen, and their growth should be inhibited to protect sperm fertilizing capacity female’s health. In pig breeding, where routinely stored at 17°C liquid state, alternatives conventional antibiotics urgently needed. Photodynamic inactivation (PDI) bacteria well-established tool medicine food industry but this technology has not been widely adopted preservation. The specific setting selectively inactivate while maintaining integrity functionality. aim study was test principle PDI boar using photosensitizer 5,10,15,20-tetrakis(N-methyl-4-pyridyl)-21H,23H-porphine (TMPyP) white light LED-setup. first step, photophysical experiments comprising singlet oxygen phosphorescence kinetics TMPyP determination triplet time revealed sufficiently high production reactive Androstar Premium extender, whereas seminal plasma acted as strong quencher. vitro with extended showed that established protocol preserves motility, membrane integrity, DNA mitochondrial activity efficiently reducing below detection limit. A proof-of-concept insemination confirmed vivo fertility after photodynamic treatment. conclusion, approach, innovative controls maintains fertility. This could promising contribution One Health concept potential reduce antimicrobial animal husbandry.

Language: Английский

Citations

2
Comparative Analysis of Microfluid Sperm Sorting Chip with Density Gradient Approaches for Intrauterine Insemination Cycles DOI Creative Commons
Vivek S Dave, Asha Arora

Published: Jan. 16, 2024

The primary goal of sperm preparation prior to IUI is eliminate viruses, antibodies, leucocytes, and debris from sperm, as well inhibitors capacitation, factors like reactive oxygen radicals, prostaglandins. Microfluidic sorting has come up an alternate tool centrifugation-based conventional procedures for in vitro fertilisation recent years. purpose this prospective study examine the impact microfluidic chip processing density gradient centrifugation on embryo development teratozoospermic patients.

Language: Английский

Citations

0
Influence of bacterial infection on human sperm DOI Creative Commons

Yousry Khalaf Sadeek,

Tarek M. Abdelghany,

Aii Gamal Gadel-Rab

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 13, 2024

Abstract Background Invasion of bacteria in to the male reproductive system associated with decrease sperm function, leading impair fertilizing ability, human body harbors trillions microbes, and their influence on health has been explored many parts body, there is a general agreement negative impact some pathogenic bacterial species semen parameters, including counts, motility, morphology, Deoxyribonucleic acid (DNA) integrity. The Aim this study was evaluate impacts microbial infection parameters functions. This work included 186 specimen from sub fertile men unselected couples consulting infertility clinic center. All samples examined bacteriologically, also quality, evaluated according world Health organization guideline (WHO,2010). Results patients investigated for infertility, 65 (34.94%) studied specimens were infected different species. strains identified as Enterococcus Fecails, staphylococcus haemolyticyus, Escherichia coli, Micrococcus lylae Serratia Marcescens. Infected had quality (count, progressive, vitality normality. Moreover, high Reactive oxygen (ROS) function which include Acrosin activity (A.A) Hypo-osmotic swelling test (HOST) noticed comparison non-infected but non-significant. Conclusion lead ability sperm.

Language: Английский

Citations

0