Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Nov. 14, 2023
Abstract
This
is
the
first
article
to
report
of
chemical
profile
leaves
Rubus
alceifolius
Poir,
a
widely
distributed
medicinal
plant
in
Eastern
Hemisphere.
Fresh
R.
were
treated
by
set
experimental
protocols
prepare
lyophilized
aqueous
extract.
A
novel
strategy
was
used
analyze
extract,
i.e.,
The
extract
then
analyzed
using
new
strategy,
database-affinity
ultra-high-performance
liquid
chromatography-quadrupole-Exactive-Orbitrap-tandem
mass
spectrometry
(UHPLC-Q-Exactive-Orbitrap-MS/MS).
Using
MS/MS
at
full
elucidation
and
comparison
with
database,
33
compounds
putatively
identified,
including
12
flavonoid
derivatives,
6
phenolic
acid
caffeoylquinic
acids,
two
tea
polyphenols,
others.
Especially,
isomers
have
been
strictly
distinguished,
apigenin
vs
2'-hydroxydaidzein,
luteolin
7-O-glucuronide
scutellarin,
(+)
catechin
(-)
epicatechin,
3
-O-
4
5
acid,
3,4
dicaffeoylquinic
3,5
4,5-
O
-dicaffeoylquinic
acid.
In
addition,
21
non-isomeric
also
found
under
both
negative
positive
ion
models,
such
as
ellagic
gallic
Structural
suggested
that
there
biogenetic
relationships
among
glycosidation
hydroxylation.
All
these
findings
will
help
understand
substance
basis
traditional
functions
Alceifolius
.
structural
between
These
allow
scientific
community
better
functions.
Molecules,
Journal Year:
2025,
Volume and Issue:
30(3), P. 722 - 722
Published: Feb. 5, 2025
There
has
been
no
chemical
analysis
of
the
fruit
medicinal
plant
Jatropha
podagrica
until
now.
The
current
study
aimed
to
qualitatively
and
quantitatively
analyze
J.
using
a
high-resolution
LC-MS
strategy,
i.e.,
library-comparison
ultra-high-performance
liquid
chromatography-Quadrupole-Orbitrap-tandem
mass
spectrometry.
strategy
putatively
identified
46
compounds
from
fresh
fruit.
During
putative
identification,
10
isomers
(e.g.,
(vitexin
vs.
isovitexin)
were
completely
distinguished
each
other.
Thereafter,
all
simultaneously
quantified
authentic
standard
comparison
method.
Finally,
they
also
subjected
2,2′-azino
bis
(3-ethylbenzothiazolin-6-sulfonic
acid
radical
(ABTS+•)-scavenging
assay
characterize
their
relative
antioxidant
capacities.
Their
capacities
thus
multiplied
by
contents
calculate
contribution
values,
respectively.
Corilagin,
gallic
acid,
ellagic
phillygenin
exhibited
highest
percentages
thereby
suggested
as
four
top
contributors.
are
recommended
build
up
quality-markers
(Q-markers)
system
fruits.
All
these
findings
can
help
develop
fruits
potential
resource
natural
medicine.
Chinese Medicine,
Journal Year:
2023,
Volume and Issue:
18(1)
Published: Sept. 9, 2023
Abstract
Wushicha
Granule,
an
over-the-counter-drug
(OTC)
prescription,
consists
of
19
traditional
Chinese
herbals
medicines
(CHMs),
such
as
Chaihu,
Hongcha,
Chuanxiong,
Houpo,
and
Gancao.
The
five
however
have
not
been
effectively
characterized
by
the
quality-markers
(Q-markers)
system
in
current
Pharmacopoeia.
study
therefore
established
a
novel
database-aided
ultra-high
performance
liquid
chromatography-quadrupole-orbitrap
mass
spectrometry
(UHPLC-Q-orbitrap
MS/MS)
strategy.
strategy
has
putatively
identified
52
compounds
from
mainly
including
flavonoids,
saponins,
alkaloid,
lignins,
lactones.
Especially,
saponin
“glycyrrhetinic
acid”
Granule
was
specifically
18β-configuration
(rather
than
18α-configuration).
Meanwhile,
two
pairs
isomers
were
fully
discriminated,
vitexin
vs
isovitexin
daidzein
7,4'-dihydroxyflavone.
8β-Glycyrrhetinic
acid,
together
with
saikosaponin
A,
alkaloid
caffeine,
lactone
S
-senkyunolide
lignin
magnolol,
further
studied
using
quantum
chemical
calculation,
UV–vis
spectra,
anti
-counterfeiting
validation
experiment.
In
experiment,
they
successfully
recognized
6
counterfeit
Granules,
means
LC–MS
equipped
extraction
software.
Based
on
these
results,
8β-glycyrrhetinic
acid
is
recommended
to
replace
old
Q-marker
acid”;
while
S-
senkyunolide
magnolol
are
new
Q-markers.
These
recommendations
can
only
recognize
counterfeits
regarding
Gancao,
but
also
prevent
possible
safety-incident.
All
will
greatly
improve
efficiency
specificity
Journal of Liquid Chromatography & Related Technologies,
Journal Year:
2023,
Volume and Issue:
46(6-10), P. 153 - 167
Published: June 21, 2023
Zibushengfa
Tablet
is
a
widely
consumed
traditional
Chinese
medicine
(TCM)
prescription.
However,
its
main
bioactives
remain
unclear
and
pharmacopeia
quality-marker
(Q-marker)
poorly
characterized
nowadays.
The
present
study
analyzed
lyophilized
aqueous
powder
using
ultra-high-performance
liquid
chromatography-quadrupole-orbitrap
mass
spectrometry.
Through
comparison
with
authentic
standards
in
the
library,
46
were
putatively
identified,
including
quinic
acid,
D-gluconic
gallic
protocatechuic
5-caffeoylquinic
L-tryptophan,
salidroside,
(+)-catechin
hydrate,
caffeic
cryptochlorogenic
mangiferin,
ferulic
myricetin-3-O-galactoside,
2,3,5,4'-tetrahydroxystilbene-2-O-glucoside,
forsythoside
B,
myricitrin,
rutin,
hyperoside,
acteoside,
3,4-dicaffeoylquinic
myricetin,
senkyunolide
A,
isochlorogenic
acid
C,
quercitrin,
(+)-epipinoresinol,
quercetin,
biochanin
calycosin,
luteolin,
wedelolactone,
calycosin-7-O-glucoside,
chrysoeriol,
trans-cinnamic
formononetin,
chrysin,
3,3',4',5,6,7,8-heptamethoxyflavone,
Z-ligustilide,
amentoflavone,
tangeretin,
hinokiflavone,
emodin,
physcion,
linoleic
stearic
palmitic
ethyl
ester.
Of
these,
four
pairs
of
isomers
successfully
differentiated
newly
established
library-filtering
strategy.
On
this
basis,
wedelolactone
are
preliminarily
suggested
as
additional
quality-markers
for
consideration
by
Pharmacopeia
Committee,
to
prevent
possible
adulterate.
Food Chemistry X,
Journal Year:
2025,
Volume and Issue:
unknown, P. 102363 - 102363
Published: March 1, 2025
This
study
systematically
analyzed
the
chemical
composition
of
vine
tea
using
LC-MS
and
GC-MS
to
determine
its
optimal
harvest
time
key
antioxidant
compounds.
By
employing
mass
defect
filtering
(MDF)
a
data
fusion
algorithm,
examined
compositional
changes
across
different
harvesting
periods.
The
results
identified
62
57
compounds
through
LC-MS,
respectively.
In
vitro
assays
revealed
that
harvested
in
July
exhibited
strongest
activity.
Correlation
analysis
further
five
compounds:
myricetin,
dihydromyricetin,
quercetin,
kaempferol,
quercitrin.
research
not
only
clarifies
primary
substances
responsible
for
tea's
properties
but
also
provides
scientific
guidance
optimizing
timing.
Furthermore,
integrated
analytical
approach
adopted
this
offers
novel
strategy
exploring
bioactivity
other
traditional
Chinese
herbal
medicines,
paving
way
advancements
field.
Journal of Separation Science,
Journal Year:
2023,
Volume and Issue:
46(13)
Published: April 27, 2023
Eucommiae
Folium
(Duzhongye)
is
a
traditional
Chinese
medicine
with
long
history
of
use
in
China.
However,
its
quality‐marker
Pharmacopoeia
poorly
defined
nowadays.
The
study,
therefore,
conducted
an
ultra‐high‐performance
liquid
chromatography
coupled
hybrid
quadrupole‐orbitrap
tandem
mass
spectrometry
analysis
to
obtain
accurate
data.
obtained
data
were
then
compared
the
authentic
standards
library
using
Xcalibur
4.1
software
package
and
TraceFinder
General
Quan.
Through
comparison,
study
has
putatively
identified
26
bioactive
compounds,
which
include
17
flavonoid
derivatives
(catechin,
quercetin
3‐gentiobioside,
3‐
O
‐β‐D‐glucose‐7‐
‐β‐D‐gentiobioside,
taxifolin,
myricetin
‐galactoside,
myricitrin,
hyperoside,
rutin,
isoquercitrin,
‐β‐xylopyranoside,
quercitrin,
isorhamnetin
‐β‐D‐glucoside,
quercetin,
kaempferol,
S
‐eriodictyol,
‐naringenin,
phloridzin),
four
caffeoylquinic
acids
(neochlorogenic
acid,
chlorogenic
isochlorogenic
acid
A,
C),
two
alkaloids
(vincamine
jervine),
one
lignan
(pinoresinol),
xanthone
(cowaxanthone
B),
steroid
(cholesteryl
acetate).
Of
these,
isoquercitrin
recommended
as
new
additional
pharmacopeia
candidate,
can
not
only
overcome
unreliability
old
but
also
recognize
possible
counterfeit.
Molecules,
Journal Year:
2024,
Volume and Issue:
29(6), P. 1380 - 1380
Published: March 20, 2024
Moringa
oleifera
Lam,
commonly
known
as
moringa,
is
a
plant
widely
used
both
human
food
and
for
medicinal
purposes
around
the
world.
This
research
aimed
to
evaluate
efficacy
of
aqueous
extract
leaves
(MoAE)
benzyl
isothiocyanate
(BIT)
in
rats
with
induced
breast
cancer.
Cancer
was
7,12-dimethylbenz[a]anthracene
(DMBA)
at
dose
60
mg/kg
by
orogastric
gavage
once
only.
Forty-eight
were
randomly
assigned
eight
groups,
each
consisting
six
individuals.
The
control
group
(healthy)
called
Group
I.
II
received
DMBA
plus
saline.
In
addition
DMBA,
Groups
III,
IV,
V
MoAE
100,
250,
500
mg/kg/day,
respectively,
while
VI,
VII,
VIII
BIT
5,
10,
20
respectively.
Treatment
carried
out
13
weeks.
Secondary
metabolite
analysis
results
identified
predominantly
quercetin,
caffeoylquinic
acid,
neochlorogenic
vitexin,
kaempferol,
well
tropone,
betaine,
loliolide,
vitexin.
administration
exhibited
notable
decrease
total
tumor
count
cumulative
weight,
along
delay
their
onset.
Furthermore,
they
improved
histological
grade.
A
significant
serum
levels
VEGF
IL-1β
observed
(p
<
0.001)
better
effect
demonstrated
mg/kg.
conclusion,
this
study
suggests
that
possess
antitumor
properties
against
mammary
carcinogenesis,
could
be
due,
least
part,
flavonoids
isothiocyanates
present
extract.
Molecules,
Journal Year:
2024,
Volume and Issue:
29(6), P. 1392 - 1392
Published: March 20, 2024
Naodesheng
Tablet
(Naodesheng
Pian),
a
traditional
Chinese
medicine
formula
for
stroke
treatment,
is
made
up
of
five
herbal
medicines,
i.e.,
Sanqi,
Gegen,
Honghua,
Shanzha,
and
Chuanxiong.
However,
the
current
Pharmacopoeia
quality-marker
(Q-marker)
system
cannot
detect
possible
adulteration.
Our
study
tried
to
use
new
strategy,
standards-library-dependent
ultra-high-performance
liquid
chromatography-quadrupole-Orbitrap
mass
spectrometry
(UHPLC-Q-Orbitrap
MS/MS)
putative
identification,
reconstruct
Q-marker
system.
Through
30
isomers
were
successfully
differentiated
(such
as
2′-hydroxygenistein,
luteolin,
kaempferol;
ginsenoside
Rg2
Rg3;
Rf
Rg1).
In
particular,
11
compounds
unexpectedly
found
in
Naodesheng,
including
7,4′-dihydroxyflavone,
pectolinarigenin,
7-methoxy-4′-hydroxyisoflavone,
scoparone,
matrine,
3,3′,4′,5,6,7,8-heptamethoxyflavone,
5-hydroxyflavone,
diosgenin,
chloesteryl
acetate,
(+)-4-cholesten-3-one.
total,
68
putatively
identified
fully
elucidated
their
MS
spectra.
Subsequently,
relevant
further
investigated
using
UV-vis
scanning
experiments,
semi-quantitative
analysis,
quantum
chemical
calculation.
Finally,
adulterated
Tablets
used
validation
experiments.
The
experiment
detected
ones
via
lower-version
LC-MS
analysis.
On
this
basis,
three
candidates
(hydroxy
safflor
yellow
A
(HSYA),
citric
acid,
levistilide
A),
along
with
puerarin
notoginsenoside
R1,
are
re-nominated
Q-markers
analysis
puerarin,
HSYA,
can
clearly
adulteration
regarding
all
medicines
mentioned
above.
Therefore,
reconstructed
described
“perfect”
quality
control
will
offer
valuable
recommendation
Commission.
