The Extraction, Biosynthesis, Health-Promoting and Therapeutic Properties of Natural Flavanone Eriodictyol

Nutrients, Journal Year: 2024, Volume and Issue: 16(23), P. 4237 - 4237

Published: Dec. 8, 2024

Eriodictyol is a flavanone compound commonly found in several edible plants. Ultrasound-assisted extraction and high-performance liquid chromatography (HPLC) are used methods for the separation analysis of eriodictyol. Many studies show that some micro-organisms can produce eriodictyol as host. What more, has wide range health benefits, including skincare, neuroprotective, hypoglycemic, anti-inflammatory, antioxidant activities. In addition, therapeutic properties cardioprotective, hepatoprotective, anticancer, with protective effects on lungs kidneys, so on. This review examines extraction, biosynthesis, natural its value medicine food.

Language: Английский

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Jinhua Qinggan Granule UHPLC-Q-extractive-Orbitrap-MS assay: Putative identification of 45 potential anti-Covid-19 constituents, confidential addition, and pharmacopoeia quality-markers recommendation

Journal of Food and Drug Analysis, Journal Year: 2023, Volume and Issue: 31(3), P. 534 - 551

Published: Aug. 31, 2023

The study combined UHPLC-Q-Orbitrap-MS analysis with authentic standards, to create a novel strategy for isomers recognition and putative identification. Through the strategy, anti-Covid-19 Jinhua Qinggan Granule was found comprise 28 45 potential constituents. detection of three constituents (Danshensu, cryptotanshin, tanshinone IIA) suggests Danshen as confidential additive. Based on this, 6 are recommended quality-marker candidates, including chlorogenic acid, acteoside, peimisine, baicalein, licoricesaponin H2, IIA. Obviously, can not only help public really understand Granule's formula chemistry, but also facilitate its Pharmacopoeia collection in future.

Language: Английский

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Proposing anti‐counterfeiting pharmacopoeia quality markers for Shenlingbaizhu granule based on UHPLC‐Q‐orbitrap‐MS identification

Phytochemical Analysis, Journal Year: 2023, Volume and Issue: 35(2), P. 220 - 238

Published: Sept. 21, 2023

Abstract Introduction Shenlingbaizhu granule, a Traditional Chinese Medicine prescription comprising Renshen, Gancao, and Shanyao, is widely consumed in China nowadays. Objective The study tries to propose pharmacopoeia quality markers (Q‐markers) prevent counterfeiting involving Shanyao. Methodology A novel strategy, that is, library‐based ultra‐high‐performance liquid chromatography–quadrupole‐orbitrap mass spectrometry, was used analyse the lyophilised aqueous powder of granule. Subsequently, quantum chemistry calculation UV‐vis spectra scanning were also performed through theoretical or experimental approaches. Result Thirty‐two isomers have been strictly distinguished, especially positional isomeric isochlorogenic acid B versus C, schaftoside isoschaftoside, ginsenoside Rg2 20S ‐ginsenoside Rg3, stereoisomeric Rg3 20R Rg3. Seventeen compounds unexpectedly observed, particularly scoparone pectolinarigenin, while total 76 bioactive putatively identified study. results revealed glycyrrhizic acid, Re, Rb1, diosgenin displayed different dipole moment values maximum absorption wavelengths from each other. Conclusion recommends as four anti‐counterfeiting Q‐markers for pharmacopoeia. can be detected using conventional HPLC. observation 17 unexpected updates our knowledge regarding bioactives

Language: Английский

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Putative Identification of 47 Compounds from Jieyu Anshen Granule and Proposal of Pharmacopeia Quality‐Assessment Strategy Using TCM‐Specific Library with UHPLC‐Q‐Exactive‐Orbitrap‐MS

ChemistryOpen, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 21, 2024

Abstract Jieyu Anshen Granule is a traditional Chinese medicine prescription used for depression and primarily comprises five herbal medicines: Zhizi, Chaihu, Zhigancao, Danggui, Yuanzhi. This study established medicine‐specific library using emerging ultra‐high‐performance liquid chromatography‐quadrupole‐orbitrap‐tandem mass spectrometry analysis. Through comparison, the has fulfilled isomers distinction. As result, 47 compounds were simultaneously putatively identified from Granule, including 12 unexpected 35 expected compounds. The comprised cyclocommunol, 5‐hydroxyflavone, tangeretin, 3,5,6,7,8,3’,4’‐heptemethoxyflavone, calycosin‐7‐ O ‐β‐D‐glucoside, 7,4’‐dihydroxyflavone, naringenin‐7 ‐O‐ β‐D‐glucoside, matrine, betaine, jervine, alantolactone, hypericin. Among compounds, saikosaponin A, D, glycyrrhizic acid, geniposide, ligustilide, polygalaxanthone III further investigated quantum chemistry approach. Based on these, an effective quality assessment strategy proposed Pharmacopeia, involving simultaneous analysis of III, saikosaponins A D through enables detection adulteration in relation to Yuanzhi Granule. findings will deepen understanding

Language: Английский

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Development of Effervescent Tablets Containing Elderberry Vine Tea Compound and Evaluation of Their Antioxidant Activity

Journal of Food Processing and Preservation, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 14

Published: Dec. 7, 2023

Elderberry and vine tea, renowned for their wealth of bioactive constituents potent antioxidant prowess, hold remarkable promise within a wide array culinary contexts. This study presents pioneering advancement in the realm compound effervescent tablet formulation. Employing response surface design optimization, this work develops formulation comprising total disintegrant (acid-to-base ratio 1.3 : 1) at 50%, elderberry extracts 31.7%, ultra-micro tea powder 8.3%, stevioside 3.4%, PEG-6000 2.7%, mannitol 2.9%, PVP 1%. Under these carefully optimized conditions, resultant exhibits attributes including an average disintegration time $184.50\pm 4.65\text{s}$ , foaming quantity id="M2"> 30.64 2.10 mg / g hardness measuring id="M3"> 54.59 3.41 N with weight uniformity well ±5%. Regarding efficacy, flavonoid content attains id="M4"> 76.79 3.76 RE/g, phenolic reaches id="M5"> 94.84 4.95 GAE . Moreover, id="M6"> E C 50 values scavenging 1,1-diphenyl-2-picrohydrazine (DPPH), hydroxyl (OH), 2, 2 id="M7"> ′ -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS) radicals are determined 0.4667 mg/mL, 0.9772 0.5014 respectively. investigation underscores performance satisfying stringent physicochemical standards, while also showcasing outstanding potency, paving way antioxidant-functional foods sustainable exploitation natural resources.

Language: Английский

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Integrated Uhplc-Q-Exactive-Orbitrap Ms/Ms and Abts Analyses Screen Antioxidant and Anti-Counterfeiting Marker of Eucommiae Cortex (Duzhong) for Pharmacopoeias

Published: Jan. 1, 2024

Eucommiae Cortex (Duzhong) has been collected in the several Pharmacopoeias. However, its Pharmacopoeia quality-marker is outdated and unreasonable. The study therefore used database-aided ultra-high-performance liquid chromatography coupled with hybrid quadrupole-orbitrap tandem mass spectrometry (UHPLC-Q-Exactive-Orbitrap MS/MS) analysis, to putatively identify 33 compounds from Cortex. Especially, 14 "unexcavated" were detected out for first time; Meanwhile, all monocaffeoylquinic acid isomers (i.e., 1-O-, 3-O-, 4-O-, 5-O- caffeoylquinic acids) have strictly distinguished each other three dicaffeoylquinic (1,3-O-, 3,5-O-, 4,5-O- as well. All identified further subjected a semi-quantification through which chloesteryl acetate (4.11 ± 0.46%), sucrose (3.53 0.063%), (-)-syringaresinol (2.81 1.31%) found be highly abundant. Furthermore, results integrated ABTS+• antioxidant assay calculate contribution of individual compounds. (-)-Syringaresinol, neochlorogenic acid, vanillic chlorogenic (-) pinoresinol proven top five contributors, their values 97.50, 46.31, 40.57, 35.05, 30.90. Of was proposed additional pharmacopoeia Q-marker good testability traceability. not only enhances pharmacological relevance, but also can effectively recognize counterfeiting via analysis.

Language: Английский

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Extraction of flavonoid dyes from vine tea (Ampelopsis grossedentata) waste and application in bleached pulp dyeing

Coloration Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 2, 2024

Abstract Natural dyes are receiving more attention due to wide and renewable resources their functionalities. This study delved into the optimal conditions for extracting Ampelopsis grossedentata flavonoid (AGFDs) from vine tea waste, resulting in a dye sample with total extraction percentage of 27.4 %. Ultraviolet (UV), Fourier‐transform infrared (FTIR) liquid chromatography–mass spectrometry (LC–MS) analysis revealed presence 18 flavonoids AGFD. Additionally, various factors, including pH, light exposure, temperature, metal ions, redox agents were found impact stability Moreover, dyeing performance AGFD bleached chemical pulp was investigated. Under conditions, AGFD‐dyed displayed vibrant yellow hue excellent colour fastness when using aluminum potassium sulphate dodecahydrate (KAl(SO 4 ) 2 ·12H O) as mordant uptake level up 88 FTIR scanning electron microscopy (SEM) demonstrated that chemically bound fibre. Remarkably, hand sheet made exhibited thermal stability, physical strength properties antibacterial activity against Escherichia coli Staphylococcus aureus . Furthermore, peroxide value cheese packaged only 43 % undyed one, indicating its good antioxidant properties. highlights promising potential production functional coloured paper.

Language: Английский

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Simultaneous Quantification of 66 Compounds in Two Tibetan Codonopsis Species Reveals Four Chemical Features by Database-Enabled UHPLC-Q-Orbitrap-MS/MS Analysis

Molecules, Journal Year: 2024, Volume and Issue: 29(21), P. 5203 - 5203

Published: Nov. 3, 2024

Codonopsis canescens Nannf. (CoC) and nervosa (CoN) are two traditional Tibetan medicinal herbs (Zangdangshen), which have been widely used in the treatment of various diseases. In this study, their aerial underground parts were systematically analyzed using database-enabled UHPLC-Q-Orbitrap-MS/MS technology. This technology introduced three adduct ions, [M − H]−, + H]+, NH4]+, to putatively identify a total 66 compounds. During putative identification, at least 16 isomers successfully differentiated, such as isochlorogenic acid A vs. B C. Thereafter, all these identified compounds further quantified for contents based on linear regression method. Their observed vary from 0.00 39,127.03 µg/g. Through multiple comparisons quantification results, study found following four chemical features: (1) Four sesquiterpenes (especially atractylenolide III) enriched mainly CoC rarely CoN; (2) quinic derivatives abundant part species; (3) sixteen flavonoids (particularly diosmetin chrysoeriol) showed higher content than (4) lobetyolin was ubiquitously distributed both CoN. Based features relevant principles, (lobetyolin, III, diosmetin, recommended quality markers species. All findings can facilitate sustainable development control herbs.

Language: Английский

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Unraveling the bioactive constituents of Typha elephantina: A comprehensive phytochemical analysis by tandem mass spectrometry

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0311549 - e0311549

Published: Dec. 5, 2024

Phytochemicals derived from plants have gained significant attention in recent years due to their diverse therapeutic properties. Typha elephantina is an aquatic plant having ameliorative characteristics like antioxidant, anti-inflammatory and analgesic etc. This research aims conduct a comprehensive phytochemical investigation by Tandem mass spectrometry on the aerial parts roots of with focus identifying characterizing bioactive compounds present it. Maceration methanol, preliminary, MS/MS analyses DPPH antioxidant assay were carried out this plant. study led elucidation 62 chemical constituents for first time . 36 26 i e ., p -coumaric acid, caffeic dihydrocaffeic ferulic acid derivative, dehydroascorbic 1- O -coumaroyl glycerol, glucaroyl-4-hydroxy benzoate, apigenin 3- -glucopyranosyl isorhamnetin, isovitexin rutin, isorhamnetin diglycosides, verbascoside, forsythoside A, pinocembrin, dihydro quercetin, prunetin, ampelopsin, daidzein, genistein, catechin procyanidin B1 detected within specimen. The results showed that TE(1), TE(2) more activity than TER/MeOH. These might be responsible understanding potential provide foundation future pharmacological studies.

Language: Английский

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Database-aided UHPLC-Q-orbitrap MS/MS Strategy Putatively Identifies 52 Compounds from Wushicha Granule to Propose Anti-counterfeiting Quality-markers for Pharmacopoeia

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: July 25, 2023

Abstract Wushicha Granule, an over-the-counter-drug (OTC) prescription, consists of 19 traditional Chinese herbals medicines (CHMs), such as Chaihu, Hongcha, Chuanxiong, Houpo, and Gancao. The five however have not been effectively characterized by the quality-markers (Q-markers) system in current Pharmacopoeia. study therefore established a novel database-aided ultra-high performance liquid chromatography-quadrupole-orbitrap mass spectrometry (UHPLC-Q-orbitrap MS/MS) strategy. strategy has putatively identified 52 compounds from mainly including flavonoids, saponins, alkaloid, lignins, lactones. Especially, saponin “glycyrrhetinic acid” Granule was specifically 18β-configuration (rather than 18α-configuration). Meanwhile, two pairs isomers were fully discriminated, vitexin vs isovitexin daidzein 7,4'-dihydroxyflavone. 8β-Glycyrrhetinic acid, together with saikosaponin A, alkaloid caffeine, lactone S -senkyunolide lignin magnolol, further studied using quantum chemical calculation, UV-vis spectra, anti -counterfeiting validation experiment. In experiment, they successfully recognized 6 counterfeit Granules, means LC-MS equipped extraction software. Based on these results, 8β-glycyrrhetinic acid is recommended to replace old Q-marker acid”; while S- senkyunolide magnolol are new Q-markers. These recommendations can only recognize counterfeits regarding Gancao, but also prevent possible safety-incident. All will greatly improve efficiency specificity

Language: Английский

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