Evolution of ACE2-independent SARS-CoV-2 infection and mouse adaption after passage in cells expressing human and mouse ACE2

Virus Evolution, Journal Year: 2022, Volume and Issue: 8(2)

Published: July 1, 2022

Human

Language: Английский

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Indirect Dispersion of SARS-CoV-2 Live-Attenuated Vaccine and Its Contribution to Herd Immunity

Vaccines, Journal Year: 2023, Volume and Issue: 11(3), P. 655 - 655

Published: March 14, 2023

It has been 34 months since the beginning of SARS-CoV-2 coronavirus pandemic, which causes COVID-19 disease. In several countries, immunization reached a proportion near what is required to reach herd immunity. Nevertheless, infections and re-infections have observed even in vaccinated persons. That because protection conferred by vaccines not entirely effective against new virus variants. unknown how often booster will be necessary maintain good level protective Furthermore, many individuals refuse vaccination, developing large population yet vaccinated. Some live-attenuated are being developed. Here, we analyze indirect dispersion from their contacts contribution that this phenomenon could reaching Herd Immunity.

Language: Английский

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Development of an attenuated potato virus Y mutant carrying multiple mutations in helper-component protease for cross-protection

Virus Research, Journal Year: 2024, Volume and Issue: 344, P. 199369 - 199369

Published: April 15, 2024

Tobacco (Nicotiana tabacum) is one of the major cash crops in China. Potato virus Y (PVY), a representative member genus Potyvirus, greatly reduces quality and yield tobacco leaves by inducing veinal necrosis. Mild strain-mediated cross-protection an attractive method controlling diseases caused PVY. Currently, there lack effective stable attenuated PVY mutants. Potyviral helper component-protease (HC-Pro) likely target for development mild strains. Our previous studies showed that residues lysine at positions 124 182 (K

Language: Английский

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An inactivated SARS-CoV-2 vaccine based on a Vero cell culture-adapted high-titer virus confers cross-protection in small animals

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 24, 2024

Rapidly waning immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires continued global access to affordable vaccines. Globally, inactivated SARS-CoV-2 vaccines have been widely used during the pandemic. In this proof-of-concept study we adapted an original-D614G virus Vero cell culture as a strategy enhance vaccine manufacturing productivity. A passage 60 (P60) showed enhanced fitness and 50-fold increased yield in bioreactor compared virus. It further remained susceptible neutralization by plasma from vaccinated convalescent individuals, suggesting exposure of relevant epitopes. Monovalent P60 bivalent P60/omicron BA.1 induced neutralizing responses viruses mice hamsters, demonstrating that is suitable antigen. Antibodies cross-neutralized delta BA.5 viruses. Importantly, protected hamsters disease upon challenge with or virus, minimal lung pathology lower loads upper airways. Antigenicity was thus retained original despite acquisition adaptive mutations. Consequently, adaptation may be useful approach increase yields antigen production.

Language: Английский

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Extracellular Vesicle-Based SARS-CoV-2 Vaccine

Vaccines, Journal Year: 2023, Volume and Issue: 11(3), P. 539 - 539

Published: Feb. 24, 2023

Messenger ribonucleic acid (RNA) vaccines are mainly used as SARS-CoV-2 vaccines. Despite several issues concerning storage, stability, effective period, and side effects, viral vector widely for the prevention treatment of various diseases. Recently, vector-encapsulated extracellular vesicles (EVs) have been suggested useful tools, owing to their safety ability escape from neutral antibodies. Herein, we summarize possible cellular mechanisms underlying EV-based

Language: Английский

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NANOTECHNOLOGY AND ITS PROSPECTIVE ROLE IN USING BIOACTIVE COMPOUNDS TO FIGHT COVID 19 INFECTION IN THIS ERA

Egyptian Journal of Desert Research, Journal Year: 2023, Volume and Issue: 73(2), P. 513 - 549

Published: Dec. 20, 2023

RTIs or pneumonia, which account for 15% of all pediatric fatalities under the age five and cause significant morbidity, continue to be a serious global health issue. The most dangerous infection lately COVID-19 associated with bacterial superinfections, results in fatal acute respiratory marked by shortness breath. Scientists face challenges development vaccines antiviral antibacterial medications treat these diseases. Recently, Bioactive natural products from variety plants organisms, such as bacteria, fungi, algae, hold promise treatments cancer, infections, inflammatory diseases, other medical conditions. To facilitate transport bioactive compounds particular target, nanoparticles drug delivery system can adopted. Nanotechnology has been recognized an essential tool vast human fields. goal this review is highlight prior ongoing research on naturally occurring active nanomaterials antimicrobial agents, particularly diseases modern era like COVID-19, suggest potential new methodologies based nanotechnology platform.

Language: Английский

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Cold-adapted SARS-CoV-2 variants with different temperature sensitivity exhibit an attenuated phenotype and confer protective immunity

Vaccine, Journal Year: 2022, Volume and Issue: 41(4), P. 892 - 902

Published: Dec. 13, 2022

Language: Английский

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Review: Unravelling the Role of DNA Sensing in Alum Adjuvant Activity

Discovery Immunology, Journal Year: 2022, Volume and Issue: 2(1)

Published: Dec. 29, 2022

Public interest in vaccines is at an all-time high following the SARS-CoV-2 global pandemic. Currently, over 6 billion doses of various are administered globally each year. Most these contain Aluminium-based adjuvants (alum), which have been known and used for almost 100 years to enhance vaccine immunogenicity. However, despite historical use importance alum, we still do not a complete understanding how alum works drive In this article, critically review studies investigating mechanisms action adjuvants, highlighting some misconceptions controversies within area. Although emerged with clearer ubiquitous adjuvant works, also highlighted outstanding questions field. While may seem mainly academic interest, developing more has potential rationally modify improve immune response generated by alum-adjuvanted vaccines.

Language: Английский

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Live attenuated COVID-19 vaccines: approaches to development and prospects for clinical use

Journal of microbiology epidemiology immunobiology, Journal Year: 2023, Volume and Issue: 100(3), P. 225 - 236

Published: July 11, 2023

Although WHO declared an end to the pandemic, COVID-19 remains a significant public health concern worldwide. Modern vaccines often induce either only humoral or cellular immunity. Furthermore, new emergent epidemiologically SARS-CoV-2 variants and their spread considerably reduce effectiveness of preventive vaccination. Therefore, there is urgent need improve existing against COVID-19. One promising approaches solution problem creation "universal" vaccine that would have cross-protective activity different antigenic virus. In this respect, development live attenuated special interest, as it can activate not humoral, but also cell-mediated components immunity, providing long-term immune response cross-protection virus. This review highlights producing strains gives assessment prospects for clinical use. Some researchers use methods genetic engineering reverse genetics such site-directed mutagenesis codon deoptimization virus attenuation. Others tend traditional focusing on mutants through extended passaging in cell culture under selective conditions. The gained experience demonstrates great highly effective live-attenuated

Language: Английский

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Discovery of vaccine-like recombinant SARS-CoV-2 circulating in human

Virology Journal, Journal Year: 2022, Volume and Issue: 19(1)

Published: Dec. 8, 2022

For viral diseases, vaccination with live attenuated vaccine (LAV) is one of the most effective means for fighting diseases. However, LAV occasionally overflows from vaccinated individuals circulate in population unforeseen consequences. Currently, SARS-CoV-2 LAVs are undergoing clinical trials. In this study, we found that viruses isolated Indian SARS CoV-2 infected persons may be candidate LAV-derived strains, indicating risk spillover persons, increasing complexity detection. addition, property frequent recombination increases chance virulence reversion. Therefore, how to distinguish wild strain and avoid circulating challenges currently faced by development.

Language: Английский

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