Bacteriophage
(phage)
therapy
has
been
proposed
as
a
means
to
combat
drug-resistant
bacterial
pathogens.
Infection
by
phage
can
select
for
mutations
in
populations
that
confer
resistance
against
infection.
However,
yield
evolutionary
trade-offs
of
biomedical
use.
Here
we
report
the
discovery
staphylococcal
phages
cause
different
strains
methicillin-resistant
Staphylococcus
aureus
(MRSA)
become
sensitized
β-lactams,
class
antibiotics
which
MRSA
is
typically
highly
resistant.
cells
survive
infection
these
display
significant
reductions
minimal
inhibitory
concentration
β-lactams
compared
uninfected
bacteria.
Phage-treated
further
exhibited
attenuated
virulence
phenotypes
form
reduced
hemolysis
and
clumping.
Sequencing
analysis
revealed
evolved
unique
genetic
profiles
during
These
results
suggest
complex
trajectories
predation
open
up
new
possibilities
reduce
drug
infections.
Journal of Agriculture and Food Research,
Journal Year:
2023,
Volume and Issue:
14, P. 100708 - 100708
Published: July 11, 2023
Postbiotics
are
bioactive
substances
that
useful
and
produced
during
fermentation
in
the
matrix.
under
a
fragile
balance
our
body,
excessive
metabolities,
may
it
be
from
any
origin
has
potential
to
hamper
or
interfere
number
of
physiological
processes
body.
As
an
innovative
approach,
can
used
improve
immune
tolerance
treat
food
allergy
particularly
infants
children.
classified
on
basis
structure
(peptides,
teichoic
acis
plasmalogens),
elemental
composition
(as
carbohydrates),
proteins
(p40,
p75
molecule,
lactocepin),
vitamins
(B-group),
lipids
(butyrates,
acetate,
lactate,
propionates),
organic
acids
(such
as
3-phenyllactic
acid
propionic)
different
complex
molecules
lipoteichoic
acids,
peptidoglycan-derived
muropeptides).
Postbiotics,
mentioned
above
have
been
shown
immunological
effects
by
increasing
mucin
levels
encouraging
production
claudin
occluding.
Moreover,
studies
highlight
postbiotics
promising
remedy
for
prevention
gastrointestinal
diseases
is
also
considered
treatment
technique
with
lesser
unfavourable
side
effects,
especially
children
infants.
However,
future
head-to-head
will
necessary
determine
best
parent
cell
strains,
postbiotic
dosages,
inexpensive
comparison
other
medications.
The
source,
origin,
extraction,
synthesis,
bioactivity
mechanism
all
included
review.
antibacterial
mechanisms
discussed
this
review,
along
their
absorption
healthy
substances,
cancer,
mental
health,
therapeutic
qualities.
Infection and Drug Resistance,
Journal Year:
2023,
Volume and Issue:
Volume 16, P. 7641 - 7662
Published: Dec. 1, 2023
Antimicrobial
resistance
(AMR)
represents
a
major
threat
to
global
health.
Infection
caused
by
Methicillin-resistant
Staphylococcus
aureus
(MRSA)
is
one
of
the
well-recognized
public
health
problem
globally.
In
some
regions,
as
many
90%
S.
infections
are
reported
be
MRSA,
which
cannot
treated
with
standard
antibiotics.
WHO
reports
indicated
that
MRSA
circulating
in
every
province
worldwide,
significantly
increasing
risk
death
64%
compared
drug-sensitive
forms
infection
attributed
its
antibiotic
resistance.
The
emergence
and
spread
antibiotic-resistant
strains
have
contributed
increased
prevalence
both
healthcare
community
settings.
methicillin
due
expression
penicillin-binding
protein
2a
(PBP2a),
renders
it
impervious
action
β-lactam
antibiotics
including
methicillin.
other
through
production
beta-lactamases.
Although
treatment
options
for
limited,
there
promising
alternatives
combat
infections.
Innovative
therapeutic
strategies
wide
range
activity
modes
yet
explored.
review
highlights
challenges
posed
elucidates
mechanisms
underlying
development,
explores
mitigation
strategies.
Furthermore,
focuses
on
alternative
therapies
such
bacteriophages,
immunotherapy,
nanobiotics,
antimicrobial
peptides,
emphasizing
their
synergistic
effects
efficacy
against
MRSA.
By
examining
these
approaches,
this
provides
insights
into
potential
tackling
combatting
escalating
AMR.
Ultimately,
multifaceted
approach
encompassing
conventional
novel
interventions
imperative
mitigate
impact
ensure
sustainable
future
healthcare.
Macromolecular Bioscience,
Journal Year:
2022,
Volume and Issue:
22(10)
Published: Aug. 13, 2022
Abstract
Chronic
wounds
are
associated
with
infectious
microbial
complex
communities
called
biofilms.
The
management
of
chronic
wound
infection
is
limited
by
the
complexity
selecting
an
appropriate
antimicrobial
dressing
antibiofilm
activity
due
to
resistance
in
Herein,
situ
developed
bacterial
cellulose/poly(vinyl
alcohol)
(BC–PVA)
composite
ex
modified
genipin‐crosslinked
silk
sericin
(SS)
and
azithromycin
(AZM)
(SSga).
evaluated
as
a
material
for
preventing
development,
dispersion,
and/or
eradication
biofilm.
Fourier
transform
infrared
spectroscopy
confirms
intermolecular
interactions
between
components
BC–PVA@SSga
scaffolds.
addition
PVA
during
BC
production
significantly
increases
porosity
from
53.5%
±
2.3%
83.5%
2.9%,
pore
size
2.3
1.9
16.8
4.5
µm,
fiber
diameter
35.5
10
120
27.4
nm,
improves
thermal
stability
flexibility.
Studies
using
bacteria
fungi
indicate
high
inhibition
disruption
biofilms
upon
AZM
addition.
In
vitro
biocompatibility
analysis
nontoxic
nature
toward
HaCaT
NIH3T3
cells,
whereas
SS
enhances
cell
proliferation.
accelerates
healing
infected
mouse
model,
thus
can
be
promising
biomaterial.
Biofilm,
Journal Year:
2023,
Volume and Issue:
6, P. 100147 - 100147
Published: Aug. 2, 2023
Chronic
wound
management
is
extremely
challenging
because
of
the
persistence
biofilm-forming
pathogens,
such
as
Pseudomonas
aeruginosa
and
Staphylococcus
aureus,
which
are
prevailing
bacterial
species
that
co-infect
chronic
wounds.
Phage
therapy
has
gained
an
increased
interest
to
treat
biofilm-associated
infections,
namely
when
combined
with
antibiotics.
Here,
we
tested
effect
gentamicin
a
co-adjuvant
phages
in
dual
species-biofilm
model
formed
on
artificial
dermis.
The
biofilm-killing
capacity
treatments
was
significantly
were
applied
multiple
times
dose
(three
doses,
every
8
h).
Our
results
suggest
gentamycin
effective
adjuvant
phage
particularly
simultaneously
three
consecutive
doses.
simultaneous
treatment
seems
be
essential
avoid
resistance
development
each
antimicrobial
agents.
mSystems,
Journal Year:
2024,
Volume and Issue:
9(8)
Published: July 11, 2024
ABSTRACT
Multidrug-resistant
Staphylococcus
aureus
is
one
of
the
most
clinically
important
pathogens
in
world,
with
infections
leading
to
high
rates
morbidity
and
mortality
both
humans
animals.
The
ability
S.
form
biofilms
protects
cells
from
antibiotics
promotes
transfer
antibiotic
resistance
genes;
therefore,
new
strategies
aimed
at
inhibiting
biofilm
growth
are
urgently
needed.
Probiotic
species,
including
Bacillus
subtilis,
gaining
interest
as
potential
therapies
against
for
their
reduce
colonization
virulence.
Here,
we
search
strains
microbially
derived
compounds
strong
antibiofilm
activity
multidrug-resistant
by
isolating
screening
a
variety
agricultural
environments.
From
total
1,123
environmental
isolates,
identify
single
strain
B.
subtilis
6D1,
potent
inhibit
growth,
disassemble
mature
biofilm,
improve
sensitivity
through
an
Agr
quorum
sensing
interference
mechanism.
Biochemical
molecular
networking
analysis
active
organic
fraction
revealed
multiple
surfactin
isoforms,
uncharacterized
peptide
was
driving
this
activity.
Compared
commercial
high-performance
liquid
chromatography
grade
obtained
,
show
these
6D1
peptides
significantly
better
formation
all
four
S
.
backgrounds
preventing
-induced
cytotoxicity
when
applied
HT29
human
intestinal
cells.
Our
study
illustrates
exploring
microbial
diversity
discover
novel
agents
that
may
help
combat
enhance
efficacy
clinical
veterinary
settings.
IMPORTANCE
bacterial
pathogens,
such
increases
microorganisms’
virulence
decreases
common
regimens.
Probiotics
possess
strain-specific
competing
organisms;
however,
mechanisms
responsible
phenomena
often
go
uncharacterized.
In
study,
identified
mixture
small
probiotic-derived
capable
This
collection
also
improved
killing
protected
gut
epithelial
toxicity
stimulating
adaptive
cytokine
response.
We
conclude
purposeful
selection
efforts
can
be
used
unique
probiotic
specially
desired
action.
information
further
our
understanding
ways
which
prevent
or
Cell Reports,
Journal Year:
2023,
Volume and Issue:
42(9), P. 113154 - 113154
Published: Sept. 1, 2023
Bacteria
use
quorum
sensing
(QS)
to
coordinate
group
behavior
in
response
cell
density,
and
some
bacterial
viruses
(phages)
also
respond
QS.
In
Staphylococcus
aureus,
the
agr-encoded
QS
system
relies
on
accumulation
of
auto-inducing
cyclic
peptides
(AIPs).
Other
staphylococci
produce
AIPs
which
many
inhibit
S.
aureus
agr.
We
show
that
agr
induction
reduces
expression
tarM,
encoding
a
glycosyltransferase
responsible
for
α-N-acetylglucosamine
modification
major
phage
receptor,
wall
teichoic
acids.
This
allows
lytic
Stab20
related
phages
infect
kill
aureus.
However,
mixed
communities,
producers
inhibitory
like
haemolyticus,
caprae,
pseudintermedius
agr,
thereby
impeding
infection.
Our
results
demonstrate
cross-species
interactions
dramatically
impact
susceptibility.
These
likely
influence
microbial
ecology
efficacy
medical
biotechnological
applications
such
as
therapy.
