Microbial exposure across life reduces susceptibility of Aedes aegypti to Zika virus by enhancing blood digestion and limiting midgut cell infection DOI Creative Commons
William Louie, Ana L. Ramírez,

Rochelle Leung

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Nov. 10, 2022

The worldwide expansion of mosquito-borne pathogens necessitates improved control measures, including approaches to reduce transmission by mosquito vectors. Reducing is challenging because determinants vector competence for viruses like Zika (ZIKV) are poorly understood. Our previous work established that Aedes (Ae.) aegypti larvae reared in environmental water containing microbes less susceptible ZIKV as adults compared cohorts laboratory tap with fewer microbial species and lower abundance. Here, we identify a process which environment-derived susceptibility Ae. ZIKV. Provided the midgut represents first barrier infection, hypothesized exposure modulates infection Since mosquitoes live pupae then transition air adults, also define stage life when reduces susceptibility. were treated antibiotics during pupal adult stages, only, or provided no at any stage. Vector was next evaluated ingested ZIKV-spiked bloodmeals. Antibiotic reduced microbiota showed enhanced rates both adults. treatment disrupt resulted increased epithelium permeability, higher numbers ZIKV-infected cells, impaired bloodmeal digestion. Parallel experiments antibiotic-treated gnotobiotic dysbiotic state created antibiotic use does not influence permeability more than bacterial responsible reducing competence. disrupted via bloodmeals expression genes associated digestion metabolism relative whose antibiotics. Together, these data show throughout restricts facilitating blood cell infection. Understanding connections between microbiota, physiology, arbovirus can lead novel decrease will improve understanding habitats microbes. Author Summary Mosquito-transmitted continue threaten human health. Absent vaccines treatments, controlling limiting their ability transmit primary way prevent viral diseases. role play shaping virus has been limited association-based studies. prior develop bacteria bacteria. Here explains this association. aquatic terrestrial need be present We used water-derived only stages observed disruption increases impairs These findings advance microbiota-mosquito-virus interactions further implicate means restrict mosquitoes.

Language: Английский

Comparative analysis of bacterial microbiota in Aedes aegypti (Diptera: Culicidae): insights from field and laboratory populations in Colombia DOI
Marcela Gómez, David R. Martinez, Nicolás Luna

et al.

Journal of Medical Entomology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

Abstract Comparative studies of the microbiota in whole-body mosquitoes from natural populations and laboratory-reared specimens are scarce, particularly tropical countries like Colombia, where understanding microbial patterns is critical for effective disease control vector management. This study examines bacterial Aedes aegypti by comparing field-collected 3 Colombian regions (Southern Amazon, Central Andean region, Northern Caribbean coast) with laboratory strains (Rockefeller, Otanche, Tolima). These highly endemic dengue associated lineage 1 Ae. aegypti, known its elevated competence. Using next-generation sequencing 16S rRNA gene Illumina technology, we found that exhibited significantly higher alpha beta diversity compared to specimens. Field were enriched families such as Acetobacteraceae, Lactobacillaceae, Bacillaceae, while showed a greater abundance Enterobacteriaceae. Differential analysis revealed Acetobacter Bacillus predominated field mosquitoes, whereas Asaia, Cedacea, Chryseobacterium more common Notably, Pseudomonas Acinetobacter consistently abundant across all samples. Our findings suggest environmental factors, habitat diet, influence composition Colombia. Future research should further explore how these alongside genetic components, shape mosquito–microbiota interactions their implications transmission

Language: Английский

Citations

1
Influence of microbiota-driven natural antibodies on dengue transmission DOI Creative Commons

Alejandra Wu‐Chuang,

Alejandra Rojas, Cynthia Bernal

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: March 15, 2024

Dengue has had a significant global health impact, with dramatic increase in incidence over the past 50 years, affecting more than 100 countries. The absence of specific treatment or widely applicable vaccine emphasizes urgent need for innovative strategies. This perspective reevaluates current evidence supporting concept dual protection against dengue virus (DENV) through natural antibodies (NAbs), particularly anti-α-Gal induced by host’s gut microbiome (GM). These serve purpose. Firstly, they can directly identify DENV, as mosquito-derived viral particles have been observed to carry α-Gal, thereby providing safeguard human infections. Secondly, possess potential impede development vector interacting vector’s and triggering infection-refractory states. intricate interplay between GM NAbs on one side DENV other suggests novel approach, using target simultaneously disrupt decrease pathogen transmission competence, blocking cycles.

Language: Английский

Citations

6
Mosquito-borne bacterial communities are shaped by their insect host species, geography and developmental stage DOI Creative Commons
Saria Otani, Federica Lucati, Robert T. Eberhardt

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Abstract Mosquitoes harbour diverse bacterial communities that are influenced by a number of factors, e.g., geography, host species, and developmental stage. Understanding these microbiotas is crucial for assessing their role as vectors in pathogen dissemination. Here, we compared the Aedes albopictus japonicus across eight countries: six Europe, plus USA Japan, from both adults larval stages when possible, using 16S rRNA amplicon sequencing. We found large differences microbiota composition between mosquito with Ae. exhibiting lower diversity than japonicus. Geographic variation was also evident, mosquitoes Japan Netherlands harbouring most communities, while Austrian populations displayed lowest diversity. Developmental stage (adults larvae) had strongest influence on composition, aquatic-associated genera such Limnohabitans Hydrogenophaga dominating larvae, whereas adult harboured higher abundances Acinetobacter Methylobacterium. No association Aedes species genetic distance, determined relatedness, community compositions. A known pathogenic potential, including Pseudomonas, Serratia, Klebsiella, Acinetobacter, were detected multiple locations, suggesting could serve environmental reservoirs opportunistic antimicrobial-resistant bacteria. These findings provide insights into ecological epidemiological implications emphasize need further investigation transmission antimicrobial resistance

Language: Английский

Citations

0
Horizons of the Future: Preparedness and Response DOI
Luísa Maria Inácio da Silva, Rafael Maciel‐de‐Freitas, Marcelo Henrique Santos Paiva

et al.

Current topics in microbiology and immunology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Biotechnological Potential of Microorganisms for Mosquito Population Control and Reduction in Vector Competence DOI Creative Commons
Ricardo de Melo Katak, Amanda Montezano Cintra, Bianca Burini Kojin

et al.

Insects, Journal Year: 2023, Volume and Issue: 14(9), P. 718 - 718

Published: Aug. 22, 2023

Mosquitoes transmit pathogens that cause human diseases such as malaria, dengue fever, chikungunya, yellow Zika and filariasis. Biotechnological approaches using microorganisms have a significant potential to control mosquito populations reduce their vector competence, making them alternatives synthetic insecticides. Ongoing research has identified many can be used effectively disease transmission. However, the successful implementation of these newly proposed requires thorough understanding multipronged microorganism–mosquito–pathogen–environment interactions. Although much been achieved in discovering new entomopathogenic microorganisms, antipathogen compounds, mechanisms action, only few turned into viable products for control. There is discrepancy between number with development insecticides and/or actual available products, highlighting need investments intersection basic biotechnology.

Language: Английский

Citations

9
MosAIC: An annotated collection of mosquito-associated bacteria with high-quality genome assemblies DOI Creative Commons
Aidan Foo, Laura E. Brettell, Holly L. Nichols

et al.

PLoS Biology, Journal Year: 2024, Volume and Issue: 22(11), P. e3002897 - e3002897

Published: Nov. 15, 2024

Mosquitoes transmit medically important human pathogens, including viruses like dengue virus and parasites such as Plasmodium spp., the causative agent of malaria. Mosquito microbiomes are critically for ability mosquitoes to disease-causing agents. However, while large collections bacterial isolates genomic data exist vertebrate microbiomes, vast majority work in date is based on 16S rRNA gene amplicon that provides limited taxonomic resolution no functional information. To address this gap facilitate future studies using experimental microbiome manipulations, we generated a Mos quito- A ssociated I solate C ollection (MosAIC) consisting 392 with extensive metadata high-quality draft genome assemblies publicly available, both sequence data, use by scientific community. MosAIC encompasses 142 species spanning 29 families, members Enterobacteriaceae comprising 40% collection. Phylogenomic analysis 3 genera, Enterobacter , Serratia Elizabethkingia reveal lineages mosquito-associated bacteria isolated from different mosquito multiple laboratories. Investigation into species’ pangenomes further reveals clusters genes specific these lineages, which interest test functions connected host association. Altogether, describe generation physical collection isolates, their analyses selected groups context closely related providing unique, highly valuable resource research colonisation adaptation within hosts. Future efforts will expand include broader geographic representation, especially individuals collected field populations, well other microbes, fungi, archaea, protozoa.

Language: Английский

Citations

2
The potentiality of Culex pipiens (Diptera: Culicidae) complex holobiont in transmitting the hepatitis C virus (HCV) with the aid of bacterial microbiota in the midgut DOI Creative Commons
Fatma I. Abdallah, Bouthaina A. Merdan,

Fatma Shaarawi

et al.

Beni-Suef University Journal of Basic and Applied Sciences, Journal Year: 2024, Volume and Issue: 13(1)

Published: Nov. 25, 2024

Abstract Background The possibility of considering Culex pipiens ( Cx . ) as a vector for transmitting the hepatitis C virus (HCV) is controversial and needs further investigation. This work aimed to detect transmission by Cx. role bacterial microbiota in their midgut. Main body Two groups symbiotic aposymbiotic female were infected with using an artificial feeding membrane technique. viral load was detected head region up 60 min group 90 group. midgut can harbor 5 days after feeding. absence gut increases potentiality ten females carry high titer infective dose (1.2 × 10 6 IU/ml) HCV genotype 4a days. appears play significant HCV. On other hand, not salivary glands either or mosquitoes during detection period. transmit from adult HCV-negative human blood vitro. biological parameters survival rate, feeding, oviposition, hatchability rates, mean digestion period infection showed changes. Moreover, ex vivo assay demonstrated that HCV-infected could RNA into healthy blood. Conclusion results suggested mosquitoes’ mechanical reasonable. In addition, mosquito microbiome played determining disseminating infections. Graphic abstract

Language: Английский

Citations

2
Biotechnological Potential of Microorganisms for Mosquito Population Control and Reduction of Vector Competence DOI Open Access
Ricardo de Melo Katak, Amanda Montezano Cintra, Bianca Burini Kojin

et al.

Published: June 23, 2023

Mosquitoes transmit pathogens that cause human diseases such as malaria, Dengue fever, Chikungunya, yellow Zika and filariasis. Chemical pesticides are effective instruments for reducing disease transmission managing mosquito populations. However, these chemicals have drawbacks high production costs negative effects on the environment non-target organisms. Furthermore, mosquitoes develop resistance to chemical insecticides, they become less effective. As a result, researchers investigating novel insecticides. Advances in microbial biotechnology resulted wide range of value-added products, including biopesticides agricultural public health purposes. In this review, we highlight mainly untapped biotechnological potential microorganisms vector control reduction. Also, underline importance more basic research improved translational methodologies, encouraging efforts bridge gap between academic interventions.

Language: Английский

Citations

4
Endophytic fungus Alternaria macrospora: A promising and eco-friendly source for controlling Aedes aegypti and its toxicity assessment on non-targeted organism, zebrafish (Danio rerio) embryos DOI
Chinnasamy Ragavendran, Chinnaperumal Kamaraj,

Devarajan Natarajan

et al.

Biocatalysis and Agricultural Biotechnology, Journal Year: 2023, Volume and Issue: 56, P. 103009 - 103009

Published: Dec. 29, 2023

Language: Английский

Citations

4
Exploring Natural Alkaloids from Brazilian Biodiversity as Potential Inhibitors of the Aedes aegypti Juvenile Hormone Enzyme: A Computational Approach for Vector Mosquito Control DOI Creative Commons
Renato Araújo da Costa,

Andréia do Socorro Silva da Costa,

João Augusto Pereira da Rocha

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(19), P. 6871 - 6871

Published: Sept. 29, 2023

This study explores the potential inhibitory activity of alkaloids, a class natural compounds isolated from Brazilian biodiversity, against mJHBP enzyme Aedes aegypti mosquito. mosquito is significant vector diseases such as dengue, zika, and chikungunya. The interactions between ligands at molecular level were evaluated using computational techniques docking, dynamics (MD), mechanics with generalized Born surface area (MMGBSA) free energy calculation. findings suggest that these exhibit high binding affinity enzyme, confirmed by energies obtained in simulation. Furthermore, specific residues contribute most to stability complex identified: specifically, Tyr33, Trp53, Tyr64, Tyr129. Notably, Tyr129 previously identified crucial inhibition process. observation underscores significance research insecticides mosquitoes. These results could stimulate development new control agents are more efficient environmentally friendly.

Language: Английский

Citations

2