Cytokine, Journal Year: 2023, Volume and Issue: 174, P. 156449 - 156449
Published: Dec. 22, 2023
Language: Английский
Antibiotics, Journal Year: 2024, Volume and Issue: 13(9), P. 870 - 870
Published: Sept. 11, 2024
Phage therapy, the use of bacteriophages (phages) to treat bacterial infections, is regaining momentum as a promising weapon against rising threat multidrug-resistant (MDR) bacteria. This comprehensive review explores historical context, modern resurgence phage and phage-facilitated advancements in medical technological fields. It details mechanisms action applications phages treating MDR particularly those associated with biofilms intracellular pathogens. The further highlights innovative uses vaccine development, cancer gene delivery vectors. Despite its targeted efficient approach, therapy faces challenges related stability, immune response, regulatory approval. By examining these areas detail, this underscores immense potential remaining hurdles integrating phage-based therapies into practices.
Language: Английский
Citations
17
Signal Transduction and Targeted Therapy, Journal Year: 2025, Volume and Issue: 10(1)
Published: March 5, 2025
The successful approval of peptide-based drugs can be attributed to a collaborative effort across multiple disciplines. integration novel drug design and synthesis techniques, display library technology, delivery systems, bioengineering advancements, artificial intelligence have significantly expedited the development groundbreaking drugs, effectively addressing obstacles associated with their character, such as rapid clearance degradation, necessitating subcutaneous injection leading increasing patient discomfort, ultimately advancing translational research efforts. Peptides are presently employed in management diagnosis diverse array medical conditions, diabetes mellitus, weight loss, oncology, rare diseases, additionally garnering interest facilitating targeted platforms advancement vaccines. This paper provides an overview present market clinical trial progress therapeutics, platforms, It examines key areas through literature analysis emphasizes structural modification principles well recent advancements screening, design, technologies. accelerated including peptide-drug complexes, new vaccines, innovative diagnostic reagents, has potential promote era precise customization disease therapeutic schedule.
Language: Английский
Citations
2
Viruses, Journal Year: 2024, Volume and Issue: 16(4), P. 570 - 570
Published: April 7, 2024
Phage display is a versatile method often used in the discovery of peptides that targets disease-related biomarkers. A major advantage this technology ease and cost efficiency affinity selection, also known as biopanning, to identify novel peptides. While it relatively straightforward with optimal binding affinity, pharmacokinetics selected prove be suboptimal. Therefore, careful consideration experimental conditions, including choice using vitro, situ, or vivo selections, essential generating high specificity demonstrate desirable pharmacokinetics. Specifically, combination has been proven influence biodistribution clearance peptide-conjugated nanoparticles. Additionally, marked difference properties between nanoparticles must considered. peptide depends primarily on physiochemical can modified by amino acid modifications, size shape affect both absorption distribution. Thus, optimization desired pharmacokinetic should an important biopanning strategies enable selection effectively target biomarkers vivo.
Language: Английский
Citations
6
Expert Opinion on Drug Discovery, Journal Year: 2024, Volume and Issue: 19(8), P. 887 - 915
Published: June 18, 2024
Introduction Phage display technology is a well-established versatile in vitro that has been used for over 35 years to identify peptides and antibodies use as reagents therapeutics, well exploring the diversity of alternative scaffolds another option conventional therapeutic antibody discovery. Such successes have responsible spawning range biotechnology companies, many complementary technologies devised expedite drug discovery process resolve bottlenecks workflow.
Language: Английский
Citations
6
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(4), P. 2107 - 2107
Published: Feb. 9, 2024
The steadily increasing number of drug-resistant bacterial species has prompted the search for alternative treatments, resulting in a growing interest bacteriophages. Although they are viruses infecting cells, bacteriophages an extremely important part human microbiota. By interacting with eukaryotic able to modulate functioning many systems, including immune and nervous affecting not only homeostasis organism, but potentially also regulation pathological processes. Therefore, aim this review is answer questions (i) how animal/human systems respond under physiological conditions reduced immunity, especially during infection; (ii) whether can induce negative changes brain after crossing blood-brain barrier, which could result various disorders or increase risk neurodegenerative diseases; (iii) modify gut crucial dilemma administration always beneficial rather if it may involve any risks.
Language: Английский
Citations
3
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: Sept. 24, 2024
The evolving development landscape of biotherapeutics and their growing complexity from simple antibodies into bi- multi-specific molecules necessitates sophisticated discovery engineering platforms. This review focuses on mammalian display technology as a potential solution to the pressing challenges in biotherapeutic development. We provide comparative analysis with established methodologies, highlighting key aspects technology, including genetic engineering, construction libraries, its pivotal role hit selection and/or developability engineering. delves mechanisms underpinning developability-driven via broader implications. Applications beyond antibody are also explored, alongside advancements towards function-first screening technologies, precision genome AI/ML-enhanced situating them context display. Overall, provides comprehensive overview current landscape, underscores expansive for development, addresses critical full realisation this potential, examines advances related disciplines that might impact future application technologies.
Language: Английский
Citations
3
Frontiers in Bioscience-Elite, Journal Year: 2024, Volume and Issue: 16(3)
Published: July 11, 2024
The need for rapid and cheap synthesis of large numbers chemical compounds has contributed to the emergence combinatorial chemistry (simultaneous different compounds, in contrast traditional synthesis, which each substance is produced individually). Combinatorial library methods were initially applied only peptides oligonucleotides. By now, scope these libraries expanded considerably include proteins, synthetic oligomers, small molecules, oligosaccharides. enormous variety antibodies (Abs) makes it possible detect clones able interact highly specifically with almost any natural or antigen (Ag). Phage Abs are an excellent alternative mono- polyclonal Abs, because they stable, have no disulfide bonds, much cheaper make. Monitoring various substances, including a living organism demand. Despite vast amount literature available on Ab phage display, use display determine diagnostically important Ags not been sufficiently covered. Many studies confirmed that unlike other types ensure sensitive Ag detection. Therefore, this review focuses prepare specific (allergens, disease cancer biomarkers, toxins) their application analytical systems, biosensors. diagnostics compared classical prospects shown as biosensor sensing elements. This analyzes recent advances detection by using display–based Systematic information presented about allergens, toxins detected Abs. sensor-based affordable classic tests.
Language: Английский
Citations
2
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: Oct. 10, 2024
The antibody response to vaccination and infection is a key component of the immune pathogens. Sequencing peripheral B cells may not represent complete cell receptor repertoire. Here we present method for sequencing human plasma-derived polyclonal IgG using combination mass spectrometry B-cell sequencing. We investigate Moderna Spikevax COVID-19 vaccine. From data natural vaccination, generate 12 recombinant antibodies. Six derived antibodies, including four generated with de novo protein sequencing, exhibit similar or higher binding affinities than original antibody. Neutralization tests reveal that six antibodies possess neutralizing capabilities against target antigen. This research provides insights into potential our approach in generating robust affinity neutralization capabilities. Directly examining circulating pool crucial due misrepresentations by analysis alone. an essential anti-infective response. authors discovery from plasma characterise
Language: Английский
Citations
1
Biologics, Journal Year: 2024, Volume and Issue: 4(1), P. 55 - 74
Published: Feb. 23, 2024
Thirty-four years ago, the groundbreaking work of John McCafferty and Sir Gregory Winter in developing phage display technology revolutionized discovery human antibodies, paving way for diverse applications. Since then, numerous phage-derived antibodies have been successfully developed advanced into clinical studies, resulting approval more than a dozen therapeutic antibodies. These demonstrated efficacy across spectrum medical conditions, ranging from autoimmune diseases to various cancers. In this article, we provide an in-depth review development libraries as powerful platforms antibody discovery, elucidating intricate procedures involved development. Additionally, conduct current ntibody drugs cancer treatment that using platform. Furthermore, discuss challenges inherent technology, offering insights potential solutions enhance crucial steps facilitate efficient drug field technology.
Language: Английский
Citations
1
Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15
Published: Feb. 6, 2024
Keywords: bacteriophages, glycan binding proteins, glycoside hydrolases, glycosyltransferases, modified nucleobases, self-assembling nanoparticles, vaccines
Citations
0