A retrospective study revealing complex viral diversity and a substantial burden of HPV infection in SARS-CoV-2 positive individuals, Sierra Leone DOI Creative Commons
Xiaozhou He,

Alie Brima Tia,

Qikai Yin

et al.

Virology Journal, Journal Year: 2024, Volume and Issue: 21(1)

Published: Aug. 27, 2024

The COVID-19 pandemic has underscored the critical role of sequencing technology in disease control and outbreak response. However, resource limitations challenging environments often impede such efforts low middle-income countries. This study aimed to investigate spectrum viral co-infections, particularly with human pathogens, SARS-CoV-2 positive individuals Sierra Leone using metagenomic sequencing, evaluating feasibility utilizing this for epidemiological evolutionary surveillance pathogens related public health low-income environments. We retrospectively collected analyzed 98 nasopharyngeal swab specimens from Leone. Samples were pre-processed locally transferred China via FTA cards which was performed Novaseq platform. focused on identification viruses co-infecting SARS-CoV-2, a deeper analysis significant as HPV. identified 22 taxa 20 families, including 4 viruses. Notably, 19.4% samples showed HPV co-infection 34 distinct types, predominantly beta gamma HPVs. Multiple types found individual samples, indicating high complexity co-infections. wide range viruses, multiple genotypes, highlights interactions their potential implications health. These findings enhance our understanding co-infections provide valuable insights interventions Further research is needed explore clinical significance these impact outcomes.

Language: Английский

Genetic characteristics of anelloviruses detected in individual viromes of children with acute respiratory symptoms using the metagenomic approach DOI Creative Commons
Alina R. Nokhova, Nikita Dubovitskiy, Anastasiya Derko

et al.

Acta Virologica, Journal Year: 2025, Volume and Issue: 69

Published: March 21, 2025

More than 20 years after the discovery of first anelloviruses (AVs), they still remain a mysterious group viruses. To date, there is no clear understanding their impact on host organism, although information emerging about participation in various pathologies. Unfortunately, widespread distribution AVs makes it difficult to determine significance. However, well known that are an important component human virome. We assessed abundance viromes from four individual nasal and pharyngeal samples children with respiratory symptoms using metagenomic approach. Three were positive for bocavirus, influenza virus A syncytial virus, while one sample was negative any viruses standard PCR diagnosis. detected all samples, namely Torque teno mini (TTMV). The greatest diversity [Torque (TTV), TTMV midi (TTMDV)] observed diseased patient who had absence It suggested activated immune system combat main pathogens, against background which number decreases, may explain reduced anellome other patients. also compared results workflows qPCR major viruses, agreement. In addition, sequencing made possible obtain additional data viral genomes order establish taxonomic identification characterize profiles

Language: Английский

Citations

0
Genetic Characteristics of Anelloviruses Detected in Individual Viromes of Children with Acute Respiratory Infection Using the Metagenomic Approach DOI Open Access
Alina R. Nokhova, Nikita Dubovitskiy, Anastasiya Derko

et al.

Published: April 18, 2024

More than 20 years after the discovery of first anelloviruses (AVs), they still remain a mysterious group viruses. To date, there is no clear understanding their impact on host organism, although information emerging about participation in various pathologies. However, it well known that AVs are an important component human virome. In current study we assessed abundance viromes from four nasal and pharyngeal samples children with respiratory symptoms using metagenomic approach. It worth noting three patients had viruses one did not have any. Regarding AVs, were detected all samples, namely Torque teno mini virus (TTMV). The greatest diversity (Torque (TTV), TTMV midi (TTMDV)) shown diseased patient absence viruses, which may indicate potential involvement maintenance symptoms. An activated immune system to combat main pathogens, against background number decreases, discussed as possible reason for reduced anellome other patients. We also compared results approach qPCR major agreement. addition, sequencing made obtain additional data viral genomes order establish taxonomic identification characterize individual profiles Siberian part Russia.

Language: Английский

Citations

1
The Emerging Tool of the Human Anellovirome DOI Creative Commons
Daniele Focosi, Pietro Giorgio Spezia, Fabrizio Maggi

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(6), P. 990 - 990

Published: June 20, 2024

The blood virome is dominated by the Anelloviridae family, which emerges early in life; anellome, represents variety of anelloviruses within an individual, stabilizes adulthood [...].

Language: Английский

Citations

1
A retrospective study revealing complex viral diversity and a substantial burden of HPV infection in SARS-CoV-2 positive individuals, Sierra Leone DOI Creative Commons
Xiaozhou He,

Alie Brima Tia,

Qikai Yin

et al.

Virology Journal, Journal Year: 2024, Volume and Issue: 21(1)

Published: Aug. 27, 2024

The COVID-19 pandemic has underscored the critical role of sequencing technology in disease control and outbreak response. However, resource limitations challenging environments often impede such efforts low middle-income countries. This study aimed to investigate spectrum viral co-infections, particularly with human pathogens, SARS-CoV-2 positive individuals Sierra Leone using metagenomic sequencing, evaluating feasibility utilizing this for epidemiological evolutionary surveillance pathogens related public health low-income environments. We retrospectively collected analyzed 98 nasopharyngeal swab specimens from Leone. Samples were pre-processed locally transferred China via FTA cards which was performed Novaseq platform. focused on identification viruses co-infecting SARS-CoV-2, a deeper analysis significant as HPV. identified 22 taxa 20 families, including 4 viruses. Notably, 19.4% samples showed HPV co-infection 34 distinct types, predominantly beta gamma HPVs. Multiple types found individual samples, indicating high complexity co-infections. wide range viruses, multiple genotypes, highlights interactions their potential implications health. These findings enhance our understanding co-infections provide valuable insights interventions Further research is needed explore clinical significance these impact outcomes.

Language: Английский

Citations

0