International Immunopharmacology, Journal Year: 2024, Volume and Issue: 138, P. 112562 - 112562
Published: June 26, 2024
Language: Английский
Biomedicines, Journal Year: 2024, Volume and Issue: 12(1), P. 123 - 123
Published: Jan. 7, 2024
Lung cancer demands innovative approaches for early detection and targeted treatment. In addressing this urgent need, exosomes play a pivotal role in revolutionizing both the treatment of lung cancer. Their remarkable capacity to encapsulate diverse range biomolecules, traverse biological barriers, be engineered with specific targeting molecules makes them highly promising diagnostic markers precise drug delivery cells. Furthermore, an in-depth analysis exosomal content biogenesis offers crucial insights into molecular profile tumors. This knowledge holds significant potential development therapies strategies Despite notable progress field, challenges standardization cargo loading persist. Collaborative research efforts are imperative maximize advance field precision medicine benefit patients.
Language: Английский
Citations
24
Reumatologia/Rheumatology, Journal Year: 2023, Volume and Issue: 61(3), P. 191 - 201
Published: July 2, 2023
Although the pathological mechanisms involved in osteoarthritis (OA) and rheumatoid arthritis (RA) are different, onset progression of both diseases associated with several analogous clinical manifestations, inflammation, immune mechanisms. In diseases, cartilage destruction is mediated by matrix metalloproteinases (MMPs) synthesized chondrocytes synovium fibroblasts. This review aims to summarize recent articles regarding role MMPs OA RA, as well possible methods targeting alleviate degradation processes taking part RA. The novel experimental MMP-targeted treatments RA MMP inhibitors eg. 3-B2, taraxasterol, naringin. While other aim silence miRNAs, lncRNAs, or transcription factors. Additionally, MMP-related developments include gene polymorphism MMPs, which has been linked susceptibility, MMP-generated neoepitope CRP, could serve a biomarker progression.
Language: Английский
Citations
28
Stem Cells Translational Medicine, Journal Year: 2024, Volume and Issue: 13(4), P. 371 - 386
Published: Feb. 13, 2024
Abstract Acute lung injury (ALI) is an important pathological process of acute respiratory distress syndrome, yet there are limited therapies for its treatment. Mesenchymal stem cells-derived exosomes (MSCs-Exo) have been shown to be effective in suppressing inflammation. However, the effects MSCs-Exo on ALI and underlying mechanisms not well elucidated. Our data showed that MSCs-Exo, but derived from MRC-5 cells (MRC-5-Exo), which human fetal fibroblast cells, significantly improved chest imaging, histological observations, alveolocapillary membrane permeability, reduced inflammatory response mice model. According miRNA sequencing proteomic analysis MRC-5-Exo, may inhibit pyroptosis by miRNAs targeting caspase-1-mediated pathway, proteins with immunoregulation functions. Taken together, our study demonstrated were treating inhibiting alveolar macrophages reducing inflammation response. Its mechanism through pyroptosis-targeting immunoregulating delivered MSCs-Exo. Therefore, a new treatment option early stage ALI.
Language: Английский
Citations
11
Molecular Aspects of Medicine, Journal Year: 2024, Volume and Issue: 99, P. 101302 - 101302
Published: Aug. 1, 2024
Modern methods of molecular diagnostics and therapy have revolutionized the field medicine in recent years by providing more precise effective tools for detecting treating diseases. This progress includes a growing exploration body's secreted vesicles, known as extracellular vesicles (EVs), both diagnostic therapeutic purposes. EVs are heterogeneous population lipid bilayer almost every cell type studied so far. They detected body fluids conditioned culture media from living cells. play crucial role communication between cells organs, locally over long distances. recognized their ability to transport endogenous RNA proteins cells, including messenger (mRNA), microRNA (miRNA), misfolded neurodegenerative proteins, several other biomolecules. review explores dual utilization EVs, serving not only purposes but also platform delivering molecules tissues. Through an composition, biogenesis, selective cargo packaging, we elucidate intricate mechanisms behind via highlighting potential use applications. Finally, it addresses challenges outlines prospective directions clinical EVs.
Language: Английский
Citations
8
Cancers, Journal Year: 2023, Volume and Issue: 15(15), P. 3804 - 3804
Published: July 26, 2023
Glioblastoma (GBM) is a highly aggressive and lethal primary brain cancer that necessitates early detection accurate diagnosis for effective treatment improved patient outcomes. Traditional diagnostic methods, such as imaging techniques tissue biopsies, have limitations in providing real-time information distinguishing treatment-related changes from tumor progression. Liquid used to analyze biomarkers body fluids, offer non-invasive dynamic approach detecting monitoring GBM. This article provides an overview of GBM including circulating cells (CTCs), cell-free DNA (cfDNA), RNA (cfRNA), microRNA (miRNA), extracellular vesicles. It explores the clinical utility these detection, monitoring, prognosis. Challenges implementing liquid biopsy strategies practice are also discussed. The highlights potential biopsies valuable tools personalized management but underscores need standardized protocols further research optimize their utility.
Language: Английский
Citations
18
Vaccines, Journal Year: 2024, Volume and Issue: 12(3), P. 280 - 280
Published: March 7, 2024
Exosomes are small subtypes of extracellular vesicles (EVs) naturally released by different types cells into their environment. Their physiological roles appear to be multiple, yet many aspects biological activities remain understood. These can transport and deliver a variety cargoes may serve as unconventional secretory vesicles. Thus, they play crucial role important vectors for intercellular communication the maintenance homeostasis. Exosome production content vary under several stresses or modifications in cell microenvironment, influencing cellular responses stimulating immunity. During infectious processes, exosomes described double-edged swords, displaying both beneficial detrimental effects. Owing tractability, analysis EVs from multiple biofluids has become booming tool monitoring various pathologies, cancerous origins. In this review, we present an overview exosome features discuss particular ambiguous functions contexts. We then focus on properties diagnostic therapeutic tools. regard, explore capacity vectorize immunogenic viral antigens function mounting adaptive immune responses. As provide interesting platforms antigen presentation, further review available data engineering, which enables peptides interest exposed at surface. light all these data, emerging promising avenues vaccine strategies.
Language: Английский
Citations
7
International Immunopharmacology, Journal Year: 2024, Volume and Issue: 128, P. 111511 - 111511
Published: Jan. 9, 2024
Language: Английский
Citations
6
TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 176, P. 117763 - 117763
Published: May 17, 2024
In-vitro diagnostics (IVD) has become an in-demand area with a high-achievement transformation due to the expansion of new technologies. Technology grown from time-consuming laboratory techniques modern automated immunoassays and progressed real-time sensing through emergence biosensor devices. Plasmonic biosensors are one most employed optical stand out for their versatility wide range applications in diagnostic tests. One clinical areas growing interest is autoimmune diseases (AD), which have been increasingly prevalent worldwide. There rising demand reliable early AD diagnosis tools. This review highlights main challenges advanced plasmonic that exploited biomarker detection, including brief descriptions analytical performance. Finally, prospects trends this outlined, thinking about developing multiplexed platforms capable addressing multianalyte nature disease.
Language: Английский
Citations
5
Journal of Orthopaedic Surgery and Research, Journal Year: 2025, Volume and Issue: 20(1)
Published: Jan. 29, 2025
Rheumatoid arthritis (RA), a chronic inflammatory joint disease causing permanent disability, involves exosomes, nanosized mammalian extracellular particles. Circular RNA (circRNA) serves as biomarker in RA blood samples. This research screened differentially expressed circRNAs patient plasma exosomes for novel diagnostic biomarkers. In this study, samples of patients with insufficient response to methotrexate (MTX-IR), combined use tumor necrosis factor inhibitors (TNFi) were followed up half year, and 56 circRNA self-test data stratified into training, testing, external validation cohorts according whether American College Rheumatology 20% improvement criteria (ACR20) was achieved. A xgboost model developed using common hub genes identified by random forest least absolute shrinkage selection operator (LASSO), intersection derived from overlapping machine learning-selected genes. Diagnostic performance evaluated via receiver operating characteristic (ROC) curves pROC area under the curve (AUC). Optimal LASSO 4 determined, AUC > 0.6 key The performed well on test set, but not significantly set. Then, screening combination clinical data, cross-validation hsa-circ0002715, hsa-circ0001946, hsa-circ0000836, rheumatoid (RF) genes, among which hsa-circ0002715 hsa-circ0001946 emphasized markers training addition, morphology size expression CD9 CD81 verified successful extraction exosomes. qPCR analysis TNFi found that higher than who didn't reach ACR20, lower ACR20. above studies suggested may become predicting MTX-IR precision treatment.
Language: Английский
Citations
0
Advanced Therapeutics, Journal Year: 2025, Volume and Issue: unknown
Published: March 3, 2025
Abstract The rise of antibiotic‐resistant bacteria, driven by antibiotic misuse, is a major global health threat. Addressing this issue requires understanding resistance mechanisms and developing innovative solutions. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)‐associated systems (Cas), genome‐editing tool derived from prokaryotic defense mechanisms, offers precise targeting genes. By reprogramming CRISPR‐Cas, bacteria can be killed or resensitized to antibiotics through plasmid curing. However, clinical applications face challenges, particularly in delivering CRISPR‐Cas components effectively. Nanotechnology has emerged as promising approach for targeted delivery tissues cells. This paper explores the molecular resistance, emphasizing structure function their mechanisms. It highlights use nanoparticles (NPs) nanoscale carriers deliver components, reviewing recent studies that combine NPs CRISPR target Additionally, discusses current challenges future prospects field, underscoring potential nanotechnology combat resistance.
Language: Английский
Citations
0