Life Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 123746 - 123746
Published: May 1, 2025
Language: Английский
Biochemistry and Biophysics Reports, Journal Year: 2025, Volume and Issue: 41, P. 101928 - 101928
Published: Jan. 28, 2025
Language: Английский
Citations
3
Renal Failure, Journal Year: 2023, Volume and Issue: 45(1)
Published: March 20, 2023
Objectives: Diabetic nephropathy (DN) is the most common microvascular complication of diabetes mellitus. This study investigated mechanism triptolide (TP) in podocyte injury DN.Methods: DN mouse models were established by feeding with a high-fat diet and injecting streptozocin MPC5 induced high-glucose (HG), followed TP treatment. Fasting blood glucose renal function indicators, such as 24 h urine albumin (UAlb), serum creatinine (SCr), urea nitrogen (BUN), kidney/body weight ratio mice examined. H&E TUNEL staining performed for evaluating pathological changes apoptosis tissue. The markers, reactive oxygen species (ROS), oxidative stress (OS), inflammatory cytokines, nuclear factor-erythroid 2-related factor 2 (Nrf2) pathway-related proteins, pyroptosis detected Western blotting corresponding kits. cell viability evaluated MTT Hoechst 33342/PI double-fluorescence staining. Nrf2 inhibitor ML385 was used to verify regulation on Nrf2.Results: improved histopathological mice, alleviated podocytes injury, reduced OS ROS activating Nrf2/heme oxygenase-1 (HO-1) pathway, weakened inhibiting nod-like receptor (NLR) family pyrin domain containing 3 (NLRP3) inflammasome pathway. In vitro experiments further verified inhibition mediating Nrf2/HO-1 NLRP3 pathways. Inhibition reversed protective effect cells.Conclusions: Overall, via Nrf2/ROS/NLRP3 axis.
Language: Английский
Citations
31
Life Sciences, Journal Year: 2023, Volume and Issue: 322, P. 121661 - 121661
Published: April 5, 2023
Language: Английский
Citations
27
World Journal of Diabetes, Journal Year: 2023, Volume and Issue: 14(5), P. 585 - 593
Published: May 15, 2023
Diabetes mellitus (DM) is still one of the most common diseases worldwide, and its prevalence increasing globally. According to American European recommendations, metformin considered a first-line oral hypo-glycemic drug for controlling type 2 DM (T2DM) patients. Metformin ninth often prescribed in world, at least 120 million diabetic people are estimated receive drug. In last 20 years, there has been evidence vitamin B12 deficiency among metformin-treated Many studies have reported that related ma-labsorption T2DM Vitamin may very bad complication patient. this review, we will focus on effect absorption proposed mechanisms hindering absorption. addition, review describe clinical outcomes T2DM.
Language: Английский
Citations
25
Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15
Published: Feb. 22, 2024
Diabetic nephropathy (DN) represents a significant microvascular complication in diabetes, entailing intricate molecular pathways and mechanisms associated with cardiorenal vascular diseases. Prolonged hyperglycemia induces renal endothelial dysfunction damage via metabolic abnormalities, inflammation, oxidative stress, thereby compromising hemodynamics. Concurrently, fibrotic sclerotic alterations exacerbate glomerular tubular injuries. At macro level, reciprocal communication between the microvasculature systemic circulation establishes pernicious cycle propelling disease progression. The current management approach emphasizes rigorous control of glycemic levels blood pressure, renin-angiotensin system blockade conferring renoprotection. Novel antidiabetic agents exhibit renoprotective effects, potentially mediated through modulation. Nonetheless, emerging therapies present novel avenues for enhancing patient outcomes alleviating burden. A precision-based approach, coupled comprehensive strategy addressing global risk, will be pivotal mitigating burden diabetes.
Language: Английский
Citations
15
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: July 3, 2024
Ferroptosis is a form of non-apoptotic regulated cell death (RCD) that depends on iron and characterized by the accumulation lipid peroxides to lethal levels. involves multiple pathways including redox balance, regulation, mitochondrial function, amino acid, lipid, glycometabolism. Furthermore, various disease-related signaling also play role in regulating process oxidation. In recent years, with emergence concept ferroptosis in-depth study its mechanisms, closely associated biological conditions related kidney diseases, organ development, aging, immunity, cancer. This article reviews development ferroptosis, mechanisms (including GSH-GPX4, FSP1-CoQ1, DHODH-CoQ10, GCH1-BH4, MBOAT1/2 pathways), latest research progress involvement diseases. It summarizes diseases within frameworks metabolism, reactive oxygen biology, biology. The introduces key regulatory factors as well important concepts major open questions natural compounds. hoped future research, further breakthroughs can be made understanding regulation mechanism utilizing promote treatments for such acute injury(AKI), chronic disease (CKD), diabetic nephropathy(DN), renal carcinoma. paves way new approach prevent, treat clinical
Language: Английский
Citations
10
Biomolecules, Journal Year: 2022, Volume and Issue: 12(9), P. 1225 - 1225
Published: Sept. 2, 2022
Diabetic nephropathy (DN) is a common complication of diabetes mellitus. While there has been great advance in our understanding the pathogenesis DN, no effective managements this chronic kidney disease are currently available. Therefore, continuing to elucidate underlying biochemical and molecular mechanisms DN remains constant need. In regard, animal models indispensable tools. This review article highlights widely used rodent model non-obese type 2 induced by nicotinamide (NA) streptozotocin (STZ). The mechanism induction combining two chemicals involves blunting toxic effect STZ NA so that only percentage β cells destroyed remaining viable can still respond glucose stimulation. NA-STZ model, as platform for testing numerous antidiabetic renoprotective materials, also discussed. comparison with other diabetic models, such high-fat-diet/STZ genetically engineered less time-consuming expensive create. Given unique mimics certain pathological features human should continue find its applications field research.
Language: Английский
Citations
36
Aging, Journal Year: 2024, Volume and Issue: 16(4), P. 3302 - 3331
Published: Feb. 8, 2024
Objective: The exosomal cargo mainly comprises proteins, lipids, and microRNAs (miRNAs). Among these, miRNAs undertake multiple biological effects of exosomes (Exos). Some stem cell-derived have shown the potential to treat diabetic nephropathy (DN). However, there is little research into therapeutic adipose-derived cell (ADSC)-derived on DN. We aimed explore miR-204-modified ADSC-derived Exos mitigate Methods: were extracted identified from ADSCs. Histopathological injury, oxidative stress (OS), mitochondrial function, viability, apoptosis assessed For mechanism exploration, quantitative real-time polymerase chain reaction (qRT-PCR) western blotting used measure miR-204, methyltransferase (METTL3, METTL14, METTL7A), CIDEC. Also, CIDEC m6A methylation miR-204-METTL7A, METTL7A-CIDEC interactions determined. Results: Initially, OS-induced dysfunction was observed in DN rats. inhibited histopathological apoptosis, OS, similar detected vitro model. Intriguingly, miR-204 released by its upregulation enhanced anti-DN Exos. Mechanically, reduced METTL7A expression methylation, thus suppressing OS dysfunction. Conclusions: rescued inhibiting METTL7A-mediated methylation. This study first revealed significant role DN, paving way for development novel strategies improve clinical outcomes patients.
Language: Английский
Citations
8
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2024, Volume and Issue: 1870(3), P. 167022 - 167022
Published: Jan. 11, 2024
Language: Английский
Citations
6
International Urology and Nephrology, Journal Year: 2023, Volume and Issue: 56(4), P. 1449 - 1463
Published: Oct. 10, 2023
Abstract Objective The etiopathogenesis of diabetes nephropathy (DN) has not yet been fully clarified. Finding effective treatments to prevent renal failure in DN patients become the main focus research recent years. Circular RNA (circRNA) shown play a momentous role progression. Based on this, we aimed investigate potential mechanism by which urine-derived stem cell (USC)-derived exosome circRNA ATG7 (Exo-ATG7) mediates Methods Exosomes from USCs were isolated and identified. rat model was established intraperitoneally injecting 60 mg/kg streptozotocin. protein expression levels measured Western blot immunofluorescence. HE Masson staining used evaluate injury, related genes detected RT-qPCR. Results CircRNA significantly downregulated model, extracellular vesicles improved function reduced inflammation rats. However, after knocking down USCs-derived ATG7, improvement therapeutic effect rats lost. In addition, overexpression facilitated switching macrophages pro-inflammatory M1 phenotype anti-inflammatory M2 both vivo vitro. Mechanistically, upregulation can alleviate damage Importantly, promotes macrophage polarization regulating SOCS1/STAT3 signaling pathway through miR-4500. animal experiments also confirmed that USC cells, extracted exosome-treated could weaken exosomes. Conclusion Our results indicate USC-derived exosomal facilitates mediated miR-4500, thereby inhibiting
Language: Английский
Citations
12