Biomedicine, Journal Year: 2024, Volume and Issue: 14(4)
Published: Dec. 1, 2024
Introduction: Although the HaCaT keratinocyte model has been used in previous research to study effects of anti-psoriatic agents, there is still a lack comprehensive understanding mechanism imiquimod (IMQ)-induced proliferation and signal transduction psoriasis-like keratinocytes.
Language: Английский
Journal of Microbiology Immunology and Infection, Journal Year: 2023, Volume and Issue: 56(3), P. 433 - 441
Published: April 7, 2023
From January 2020 to December 2022, there was a total of 8,872,955 confirmed COVID-19 cases in Taiwan. In addition, 15,253 related deaths were reported. During these three years, the government and health authority did many efforts response this pandemic. early pandemic, Taiwan Central Epidemic Command Center established organize associated resource, develop effective policy implement strict intervention. infection control interventions, including universal mask wearing with increasing production face mask, hand hygiene, border control, introduce digital technology incorporating big data, quarantine cases, travel gathering restriction, implemented. meanwhile, two vaccines, namely MVC-COV1901 UB-612, have been developed under support government. Furthermore, taken into clinical practice after received emergency use approval. traditional Chinese medicines, NRICM101 NRICM102 showed their promising effect against SARS-CoV-2 recommended as potential therapeutic options for COVID-19. nonpharmacologic intervention help reduce infectious diseases, especially airborne/droplet-transmitted diseases. However, exhibited some adverse impacts on healthcare systems, such medical service out hospital cardiac arrest, cancer screening, HIV screening prevention services, public health, psychosocial status workers. Although outbreak infections may gradually subsided, we should keep monitoring its impact appropriately
Language: Английский
Citations
50
Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2023, Volume and Issue: 234, P. 115499 - 115499
Published: May 30, 2023
Language: Английский
Citations
10
Cancers, Journal Year: 2025, Volume and Issue: 17(8), P. 1321 - 1321
Published: April 14, 2025
Background: Multidrug resistance (MDR) in triple-negative breast cancer (TNBC) leads to treatment failure and tumor recurrence. Dysregulation of the MYC oncogene is associated with pathogenesis TNBC development chemoresistance via overexpression ATP-binding cassette (ABC) transporters. Therefore, present study, we aimed identify molecules from a natural product origin that prevent MDR by targeting signaling. Methods: The cell viability was evaluated using sulforhodamine assay. Protein levels were detected western blots or enzyme-linked immunosorbent assays. Intracellular calcein hoechst33342 accumulation assay evaluate inhibitory ability phytocompounds on drug-efflux functions ABCB1 ABCG2 Cancer Genome Atlas (TCGA) database used explore clinical genomic data. Furthermore, zebrafish xenotransplantation model bearing Dil-labeled cells applied testify vivo effects phyto-sesquiterpene lactones. Results: results study demonstrated lactones exhibited an prevention effect repressing efflux activities Mechanistic studies showed inducted apoptosis cycle G2/M arrested blocking STAT3/MYC pathway. Clinical data percentages amplification mRNA upregulated approximately two-fold higher patients than non-TNBC patients. survival alteration significantly lower as compared other subtypes. Moreover, xenograft confirmed exerted stronger growth vivo. Conclusions: In conclusion, these three promising candidates for shed light developing TNBC.
Language: Английский
Citations
0
Molecular Medicine Reports, Journal Year: 2024, Volume and Issue: 30(1)
Published: May 13, 2024
Psoriasis is a chronic inflammatory dermatological disease, and there lack of understanding the genetic factors involved in psoriasis Taiwan. To establish associations between variations psoriasis, genome‑wide association study was performed cohort 2,248 individuals with 67,440 without psoriasis. Using ingenuity pathway analysis software, biological networks were constructed. Human leukocyte antigen (HLA) diplotypes haplotypes analyzed using Attribute Bagging (HIBAG)‑R software chi‑square analysis. The present aimed to assess potential risks associated polygenic risk score (PRS) single nucleotide polymorphisms (SNPs) various human diseases assessed by phenome‑wide study. METAL used analyze datasets from China Medical University Hospital (CMUH) BioBank Japan (BBJ). results revealed 8,585 SNPs significance threshold P<5x10‑8, located within 153 genes strongly phenotype, particularly on chromosomes 5 6. This specific genomic region has been identified analyzing numerous pathways, including immune responses signaling. HLA genotype indicated strong HLA‑A*02:07 and HLA‑C*06:02 Taiwanese population. Based our PRS analysis, quantified. These are dermatological, circulatory, endocrine, metabolic, musculoskeletal, hematopoietic infectious diseases. meta‑analysis successful replication conducted BBJ. Several loci significantly susceptibility individuals. contributes determinants that play role Furthermore, it provides valuable insights into underlying etiology community.
Language: Английский
Citations
3
Medicina, Journal Year: 2023, Volume and Issue: 59(9), P. 1554 - 1554
Published: Aug. 26, 2023
COVID-19 is a highly transmittable respiratory illness caused by SARS-CoV-2, and acute lung injury (ALI) the major complication of COVID-19. The challenge in studying SARS-CoV-2 pathogenicity limited availability animal models. Therefore, it necessary to establish models that can reproduce multiple characteristics ALI study therapeutic applications. present established mouse model has features are similar syndrome investigate role ACE2 administration Chinese herbal prescription NRICM101 ALI. Mice with genetic modifications, including overexpression human (K18-hACE2 TG) absence (mACE2 KO), were intratracheally instillated hydrochloric acid. acid intratracheal instillation induced severe immune cell infiltration, cytokine storms, pulmonary disease mice. Compared K18-hACE2 TG mice, mACE2 KO mice exhibited dramatically increased levels inflammatory cytokines (IL-6 TNF-α) bronchoalveolar lavage fluid, histological evidence injury, dysregulation MAPK MMP activation. In could ameliorate progression acid-induced conclusion, provided an effective platform for researchers pathological mechanisms develop strategies ALI, COVID-19-related
Language: Английский
Citations
7
Tzu Chi Medical Journal, Journal Year: 2024, Volume and Issue: 36(2), P. 152 - 165
Published: March 25, 2024
Objectives: The protective effects and related mechanisms of Jing-Si herbal tea (JSHT) were investigated in cellular damage mediated by pro-inflammatory cytokines, including interleukin (IL)-1β, IL-6, tumor necrosis factor-α, on normal human lung fibroblast multiomic platform analysis. Materials Methods: silico high-throughput target was analyzed using pharmacophore models BIOVIA Discovery Studio 2022 with ingenuity pathway analysis software. To assess cell viability, the study utilized MTT assay technique. In addition, IncuCyte S3 ZOOM System implemented for continuous monitoring confluence JSHT-treated cytokine-injured HEL 299 cells. Cytokine concentrations determined a Quantibody Human Inflammation Array. Gene expression signaling pathways next-generation sequencing. Results: JSHT revealed canonical their networks. Glucocorticoid receptor is potential JSHT. results against inflammatory cytokines Transcriptome network analyses that induction helper T lymphocytes, TNFSF12, NFKB1-mediated relaxin signaling, G-protein coupled play important roles immune regulatory Conclusion: findings from our research indicate holds promise as therapeutic agent, potentially offering advantageous outcomes treating virus infections through various mechanisms. Furthermore, primary bioactive components justify extended antiviral drug development, especially context addressing coronavirus.
Language: Английский
Citations
2
Biomedicine, Journal Year: 2023, Volume and Issue: 13(4), P. 20 - 31
Published: Nov. 27, 2023
Gemcitabine is frequently utilized to treat pancreatic cancer. The purpose of our study was create a gemcitabine-resistant MIA-PaCa-2 cancer cell line (MIAGR100) and evaluate the anti-pancreatic efficacy HMJ-38, new quinazolinone analogue. Compared their parental counterparts, MIA-PaCa-2, established MIA-GR100 cells were less sensitive gemcitabine. viability not affected by 10, 50, or 100 nM gemcitabine concentrations. HMJ-38 reduced growth induced autophagy apoptosis. When stained with monodansylcadaverine (MDC), acridine orange (AO), terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL), shrunk, punctured membranes, produced vacuoles apoptotic bodies. Combining chloroquine (CQ) 3-Methyladenine (3-MA) dramatically viability, indicating that functions as
Language: Английский
Citations
6
International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(23), P. 15385 - 15385
Published: Dec. 6, 2022
Symptom treatments for Coronavirus disease 2019 (COVID-19) infection and Long COVID are one of the most critical issues pandemic era. In light lack standardized medications treating COVID-19 symptoms, traditional Chinese medicine (TCM) has emerged as a potentially viable strategy based on numerous studies clinical manifestations. Taiwan Chingguan Yihau (NRICM101), TCM designed medicinal formula with long history almost 500 years, demonstrated its antiviral properties through studies, yet pharmacogenomic knowledge this remains unclear. The molecular mechanism NRICM101 was systematically analyzed by using exploratory bioinformatics pharmacodynamics (PD) approaches. Results showed that there were 434 common interactions found between related genes/proteins. For network pharmacology NRICM101, interacting genes/proteins had highest associations interleukin (IL)-17 signaling pathway in Kyoto Encyclopedia Genes Genomes (KEGG) analyses. Moreover, tumor necrosis factor (TNF) to have association 30 frequently curated chemicals. Disease analyses also revealed relevant diseases infections pathology, followed cancer, digestive system disease, cardiovascular disease. human genes 2 microRNAs identified study could be used biomarkers or therapeutic options treatments. addition, dose–response profiles doses IL-6 TNF-α expressions cell cultures murine alveolar macrophages constructed provide pharmacodynamic information NRICM101. prevalent use attenuate residual syndromes chronic sequelae post-pandemic future.
Language: Английский
Citations
7
European Journal of Pharmacology, Journal Year: 2023, Volume and Issue: 960, P. 176146 - 176146
Published: Oct. 24, 2023
Language: Английский
Citations
3
Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown
Published: Feb. 15, 2024
Abstract Taiwan Chingguan Yihau (NRICM101) is a Traditional Chinese medicine (TCM) formula used to treat coronavirus disease 2019; however, its impact on epilepsy has not been revealed. Therefore, the present study evaluated anti-epileptogenic effect of orally administered NRICM101 kainic acid (KA)-induced seizures in rats and investigated possible mechanisms action. Sprague‒Dawley were (300 mg/kg) by oral gavage for 7 consecutive days before receiving an intraperitoneal injection KA (15 mg/kg). considerably reduced seizure behavior electroencephalographic induced rats. also significantly decreased neuronal loss glutamate increase increased GLAST, GLT-1, GAD67, GDH GS levels cortex hippocampus KA-treated In addition, suppressed astrogliosis (as determined GFAP expression); neuroinflammatory signaling HMGB1, TLR-4, IL-1β, IL-1R, IL-6, p-JAK2, p-STAT3, TNF-α, TNFR1 p-IκB levels, cytosolic p65-NFκB levels); necroptosis p-RIPK3 p-MLKL levels) The effects similar those carbamazepine, well-recognized antiseizure drug. Furthermore, no toxic liver kidney observed NRICM101-treated results indicate that antiepileptogenic neuroprotective through suppression inflammatory cues (HMGB1/TLR4, Il-1β/IL-1R1, IL-6/p-JAK2/p-STAT3, TNF-α/TNFR1/NF-κB) pathways (TNF-α/TNFR1/RIP3/MLKL) associated with level regulation brain innocuous. Our findings highlight promising role management epilepsy.
Language: Английский
Citations
0