The Role of Mesenchymal Stem Cells-Derived from Oral and Teeth in Regenerative and Reconstructive Medicine

Tissue and Cell, Journal Year: 2025, Volume and Issue: unknown, P. 102766 - 102766

Published: Jan. 1, 2025

Language: Английский

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Regulation and functional importance of human periodontal ligament mesenchymal stromal cells with various rates of CD146+ cells

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: March 7, 2025

Introduction Mesenchymal stromal cells (MSCs) with high expression of CD146 have superior properties for tissue regeneration. However, variability in the rate CD146+ among donors is observed. In this study, possible reasons behind human periodontal ligament MSCs (hPDL-MSCs) were explored. Methods hPDL-MSCs isolated from 22 different donors, and rates analyzed by flow cytometry. Furthermore, populations various magnetic separation. The dependency cell proliferation, viability, cycle, osteogenic differentiation on was investigated. Besides, effects factors, like density, confluence, inflammatory environment analyzed. Results exhibited between percentage ranging 3% to 67%. Higher associated higher presumably due S-phase, potential. Prolonged confluence seeding density led decline cells. surface stimulated treatment interleukin-1β tumor necrosis factor-α, inhibited interferon-γ. Conclusion These results suggest that are a promising subpopulation enhancing effectiveness MSC-based regenerative therapies, however affected which must be considered propagation their potential application vivo .

Language: Английский

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Adult Mesenchymal Stem Cells from Oral Cavity and Surrounding Areas: Types and Biomedical Applications

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(8), P. 2109 - 2109

Published: Aug. 9, 2023

Adult mesenchymal stem cells are those obtained from the conformation of dental structures (DMSC), such as deciduous and permanent teeth other surrounding tissues. Background: The self-renewal differentiation capacities these adult allow for great clinical potential. Because DMSC ectomesenchymal origin, they reveal a high capacity complete regeneration pulp, periodontal tissue, biomedical applications; their into types promotes repair in muscle cardiac, pancreatic, nervous, bone, cartilage, skin, corneal tissues, among others, with predictability success. Therefore, progenitor cells, exosomes origin areas oral cavity due to plasticity, considered fundamental pillar medicine regenerative dentistry. Tissue engineering (MSCs, scaffolds, bioactive molecules) sustains induces its multipotent immunomodulatory effects. It is vital importance guarantee safety efficacy procedures designed patients, this purpose, more trials needed increase several pathologies. Conclusion: From bioethical transcendental anthropological point view, human person unique being facilitates better personalized therapy, given higher prevalence chronic systemic diseases.

Language: Английский

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Huntington’s Disease Drug Development: A Phase 3 Pipeline Analysis

Pharmaceuticals, Journal Year: 2023, Volume and Issue: 16(11), P. 1513 - 1513

Published: Oct. 24, 2023

Huntington’s Disease (HD) is a severely debilitating neurodegenerative disorder in which sufferers exhibit different combinations of movement disorders, dementia, and behavioral or psychiatric abnormalities. The result trinucleotide repeat expansion mutation that inherited an autosomal dominant manner. While there currently no treatment to alter the course HD, are medications lessen abnormal symptoms. ClinicalTrials.gov was searched identify drugs have completed phase III drug trials for HD. described were further limited interventional studies recruiting, active not completed. In addition, all must posted update within past year. PubMed used gather information on these studies. Of nine clinical met criteria, eight involved following drugs: metformin, dextromethorphan/quinidine, deutetrabenazine, valbenazine, Cellavita pridopidine, SAGE-718, RO7234292 (RG6042). treatments, four already FDA approved. This systematic review provides resource summarizes present therapies treating this devastating condition United States.

Language: Английский

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Advances in the research of immunomodulatory mechanism of mesenchymal stromal/stem cells on periodontal tissue regeneration

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 3, 2025

Periodontal disease is a highly prevalent worldwide that seriously affects people's oral health, including gingivitis and periodontitis. Although the current treatment of periodontal can achieve good control inflammation, it difficult to regenerate supporting tissues satisfactory therapeutic effect. In recent years, due tissue regeneration ability, research on Mesenchymal stromal/stem cells (MSCs) MSC-derived exosomes has been gradually deepened, especially its ability interact with microenvironment body in complex immunoregulatory network, which led many new perspectives strategies for diseases. This paper systematically reviews immunomodulatory (including bone immunomodulation) properties MSCs their role inflammatory microenvironment, summarizes pathways mechanisms by MSC-EVs have promoted lists potential areas future research, describes issues should be considered basic direction development "cell-free therapies" regeneration.

Language: Английский

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Evidence and perspectives on miRNA, circRNA, and lncRNA in myocardial ischemia-reperfusion injury: a bibliometric study

Journal of Cardiothoracic Surgery, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 15, 2025

miRNA, circRNA, and lncRNA play crucial roles in the pathogenesis progression of myocardial ischemia-reperfusion injury (MI/RI). This study aims to provide valuable insights into lncRNA, MI/RI from a bibliometric standpoint, with goal fostering further advancements this area. The relevant literature field was retrieved Science Citation Index Expanded (SCI-E) database within Web Science. "Analyze Results" "Citation Report" functions WOS were utilized compile annual publication citation counts field. Microsoft Office Excel 2019 used organize visualize data. Furthermore, visualization analyses countries/regions, institutions, authors, keywords, references conducted using software CiteSpace. A total 858 publications included for analysis published across 297 journals, Molecular Medicine Reports contributing highest number publications. Researchers 45 countries participated studies field, those China most research hotspots primarily focus on three areas: role MI/RI, their potential as therapeutic targets, biomarkers. Among these, circular RNA, therapy target, inflammatory response, cardiomyocyte ferroptosis are likely emerge emerging trends overall development is rise. compilation area may researchers more assistance selecting directions, ultimately benefiting patients.

Language: Английский

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Emerging supramolecular and living materials in oral medicine

Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Regenerative Dentistry

IGI Global eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 123 - 154

Published: March 28, 2025

This chapter gives an overview of the emerging field regenerative dentistry with emphasis on use biological approaches to enhance repair, regeneration, and replacement injured tissues in oral cavity. The is divided into three key areas: medical applications stem cells, tissue engineering, growth factors. deals stem-cell-based regeneration dental pulp, periodontal tissue, issues related cell therapy such as ethics, regulation, technique. Furthermore, it addresses design manufacturing natural, synthetic, blended scaffolds specifically for dentistry, biocompatibility scaffolds, ability degrade a controlled manner match healing rates. also briefly summarizes factors bone well post-surgical wound after surgeries, importance appropriate delivery systems.

Language: Английский

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Insulin promotes the bone formation capability of human dental pulp stem cells through attenuating the IIS/PI3K/AKT/mTOR pathway axis

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 29, 2024

Abstract Background Insulin has been known to regulate bone metabolism, yet its specific molecular mechanisms during the proliferation and osteogenic differentiation of dental pulp stem cells (DPSCs) remain poorly understood. This study aimed explore effects insulin on formation capability human DPSCs elucidate underlying mechanisms. Methods Cell was assessed using a CCK-8 assay. phenotype analyzed by flow cytometry. Colony-forming unit-fibroblast ability multilineage potential were evaluated Toluidine blue, Oil red O, Alizarin red, Alcian blue staining. Gene protein expressions quantified real-time quantitative polymerase chain reaction Western blotting, respectively. Bone metabolism biochemical markers electrochemical luminescence chemical colorimetry. adhesion growth nano-hydroxyapatite/collagen (nHAC) observed with scanning electron microscope. regeneration micro-CT, fluorescent labeling, immunohistochemical hematoxylin eosin Results enhanced as well promoted mineralized matrix in concentration-dependent manner. 10 − 6 M significantly up-regulated differentiation-related genes proteins markedly increased secretion markers, obviously stimulated formation. However, it also inhibited expression receptors receptor substrates associated insulin/insulin-like factor-1 signaling (IIS) pathway, reduced phosphorylated PI3K ratios PI3K/total PI3K, notably total AKT, AKT mTOR. The inhibitor LY294002 attenuated responsiveness IIS/PI3K/AKT/mTOR pathway axis, suppressing promoting effect cell proliferation, Implantation treated into backs severe combined immunodeficient mice rabbit jawbone defects resulted Conclusions induces resistance effectively promotes their through gradually inducing down-regulation axis under resistant states.

Language: Английский

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Melatonin regulation and the function of the periodontal ligament: Future perspective and challenges

World Journal of Stem Cells, Journal Year: 2025, Volume and Issue: 17(1)

Published: Jan. 17, 2025

The present article reviews the emerging role of melatonin (MT) and Hippo-Yes-associated protein signaling pathway in periodontal regeneration, highlighting their potential to delay aging process ligament stem cells (PDLSCs). Oxidative stress cellular senescence are major obstacles regenerative therapies, especially an population. MT, a potent antioxidant, restores morphology, proliferation, osteogenic differentiation PDLSCs under oxidative conditions. Recent research highlights how MT enhances PDLSC stemness by upregulating Yes-associated expression, offering promising therapeutic strategy antagonize tissue degeneration. In addition, discusses growing interest probiotics as complementary approach improve oral microbiota support regeneration. integration with traditional novel approaches may pave way for innovative preventive or active treatments periodontology, aimed at reducing stress. Future needs focus on translating these findings into clinical applications promoting deeper understanding regeneration aging.

Language: Английский

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