Extracellular signals regulate the biogenesis of extracellular vesicles DOI Creative Commons
Yong Jin,

Lele Ma,

Wanying Zhang

et al.

Biological Research, Journal Year: 2022, Volume and Issue: 55(1)

Published: Nov. 26, 2022

Abstract Extracellular vesicles (EVs) are naturally released membrane that act as carriers of proteins and RNAs for intercellular communication. With various biomolecules specific ligands, EV has represented a novel form information transfer, which possesses extremely outstanding efficiency specificity compared to the classical signal transduction. In addition, extended concept transduction aspect by working collection extracellular information. Therefore, functions EVs have been extensively characterized exhibit an exciting prospect clinical applications. However, biogenesis and, in particular, regulation this process signals, essential conduct further studies support optimal utility, remain unclear. Here, we review current understanding EVs, focus on signals discuss their therapeutic value.

Language: Английский

Exosomes as mediators of intercellular crosstalk in metabolism DOI

Roi Isaac,

Felipe C.G. Reis, Wei Ying

et al.

Cell Metabolism, Journal Year: 2021, Volume and Issue: 33(9), P. 1744 - 1762

Published: Sept. 1, 2021

Language: Английский

Citations

466
Exosome Processing and Characterization Approaches for Research and Technology Development DOI Creative Commons
James J. Lai, Zoe L. Chau,

Sheng‐You Chen

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 9(15)

Published: March 25, 2022

Exosomes are extracellular vesicles that share components of their parent cells and attractive in biotechnology biomedical research as potential disease biomarkers well therapeutic agents. Crucial to realizing this is the ability manufacture high-quality exosomes; however, unlike biologics such proteins, exosomes lack standardized Good Manufacturing Practices for processing characterization. Furthermore, there a well-characterized reference exosome materials aid selection methods isolation, purification, analysis. This review informs technology development by comparing characterization recommending workflows. also provides detailed introduction exosomes, including physical chemical properties, roles normal biological processes progression, summarizes some on-going clinical trials.

Language: Английский

Citations

302
Small extracellular vesicles isolation and separation: Current techniques, pending questions and clinical applications DOI Creative Commons

Yuanwang Jia,

Yu Li, Tieliang Ma

et al.

Theranostics, Journal Year: 2022, Volume and Issue: 12(15), P. 6548 - 6575

Published: Jan. 1, 2022

Extracellular vesicles, especially small extracellular vesicles (sEVs) are now accepted as important messengers in cell-to-cell communication and a promising drug delivery platform.They involved nearly all physiological pathological processes disease diagnosis therapy.However, their heterogeneity of physicochemical properties functions is not fully understood, which hinders further clinical applications.To obtain highly bioactive sEVs with both high yield purity, will certainly facilitate future study application.This review informs up-to-date research on frequently-used cutting-edge technologies isolation makes deep comparison analysis different methods, including advantages, limitations applications.Pending questions about the inherent property these well strategies discussed.Additionally, an overview applications treatment, some on-going trials, also reviewed.

Language: Английский

Citations

172
Exosome engineering in cell therapy and drug delivery DOI Open Access
Somayeh Sadeghi, Fahimeh Ramezani Tehrani, Safa Tahmasebi

et al.

Inflammopharmacology, Journal Year: 2023, Volume and Issue: 31(1), P. 145 - 169

Published: Jan. 7, 2023

Language: Английский

Citations

163
Innate immune suppression by SARS-CoV-2 mRNA vaccinations: The role of G-quadruplexes, exosomes, and MicroRNAs DOI Creative Commons
Stephanie Seneff, Greg Nigh, Anthony M. Kyriakopoulos

et al.

Food and Chemical Toxicology, Journal Year: 2022, Volume and Issue: 164, P. 113008 - 113008

Published: April 15, 2022

The mRNA SARS-CoV-2 vaccines were brought to market in response the public health crises of Covid-19. utilization context infectious disease has no precedent. many alterations vaccine hide from cellular defenses and promote a longer biological half-life high production spike protein. However, immune is very different that infection. In this paper, we present evidence vaccination induces profound impairment type I interferon signaling, which diverse adverse consequences human health. Immune cells have taken up nanoparticles release into circulation large numbers exosomes containing protein along with critical microRNAs induce signaling recipient at distant sites. We also identify potential disturbances regulatory control synthesis cancer surveillance. These potentially causal link neurodegenerative disease, myocarditis, thrombocytopenia, Bell's palsy, liver impaired adaptive immunity, DNA damage tumorigenesis. show VAERS database supporting our hypothesis. believe comprehensive risk/benefit assessment questions them as positive contributors

Language: Английский

Citations

151
Circulating tumor nucleic acids: biology, release mechanisms, and clinical relevance DOI Creative Commons
Pavel Stejskal, Hani Goodarzi, Josef Srovnal

et al.

Molecular Cancer, Journal Year: 2023, Volume and Issue: 22(1)

Published: Jan. 21, 2023

Despite advances in early detection and therapies, cancer is still one of the most common causes death worldwide. Since each tumor unique, there a need to implement personalized care develop robust tools for monitoring treatment response assess drug efficacy prevent disease relapse.

Language: Английский

Citations

131
The role of Exosomal miRNAs in cancer DOI Creative Commons
Chuanyun Li, Tong Zhou, Jing Chen

et al.

Journal of Translational Medicine, Journal Year: 2022, Volume and Issue: 20(1)

Published: Jan. 3, 2022

Exosomal miRNAs have attracted much attention due to their critical role in regulating genes and the altered expression of virtually all cancers affecting humans (Sun et al. Mol Cancer 17(1):14, 2018). modulate processes that interfere with cancer immunity microenvironment, are significantly involved tumor growth, invasion, metastasis, angiogenesis drug resistance. Fully investigating detailed mechanism occurrence development various could help not only treatment but also prevention malignant diseases. The current review highlighted recently published advances regarding cancer-derived exosomes, e.g., sorting delivery mechanisms for RNAs. cell-to-cell communication, impacting angiogenesis, metastasis multiple biological features, were discussed. Finally, potential exosomal as diagnostic prognostic molecular markers was summarized, well usefulness detecting resistance therapeutic agents.

Language: Английский

Citations

104
The Emerging Role of Plant-Derived Exosomes-Like Nanoparticles in Immune Regulation and Periodontitis Treatment DOI Creative Commons
Zeyu Zhang, Yang Yu,

Guanxiong Zhu

et al.

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: June 10, 2022

Periodontitis is an infectious oral disease, which leads to the destruction of periodontal tissues and tooth loss. Although treatment periodontitis has improved recently, effective periodontitis-affected still a challenge. Therefore, it urgent explore new therapeutic strategies for periodontitis. Natural products show anti-microbial, anti-inflammatory, anti-oxidant bone protective effects most these natural are safe cost-effective. Among these, plant-derived exosome-like nanoparticles (PELNs), type nanocarriers repleted with lipids, proteins, RNAs, other active molecules, ability enter mammalian cells regulate cellular activities. Reports from literature indicate great potential PELNs in regulation immune functions, inflammation, microbiome, tissue regeneration. Moreover, can also be used as drug carriers enhance stability uptake vivo. Since function, regeneration key phenomena usually targeted during treatment, hold promising treatment. This review summarizes recent advances PELNs-related research that related periodontitis-destructed underlying mechanisms. We discuss existing challenges prospects application PELNs-based approaches

Language: Английский

Citations

80
Exosome-Based Drug Delivery: Translation from Bench to Clinic DOI Creative Commons
Hee Byung Koh, Hyo Jeong Kim, Shin‐Wook Kang

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(8), P. 2042 - 2042

Published: July 29, 2023

Exosome-based drug delivery is emerging as a promising field with the potential to revolutionize therapeutic interventions. Exosomes, which are small extracellular vesicles released by various cell types, have attracted significant attention due their unique properties and natural ability transport bioactive molecules. These nano-sized vesicles, ranging in size from 30 150 nm, can effectively variety of cargoes, including proteins, nucleic acids, lipids. Compared traditional systems, exosomes exhibit biocompatibility, low immunogenicity, reduced toxicity. In addition, be designed tailored improve targeting efficiency, cargo loading capacity, stability, paving way for personalized medicine precision therapy. However, despite exosome-based delivery, its clinical application remains challenging limitations exosome isolation purification, efficiency insufficient targeted rapid elimination circulation. This comprehensive review focuses on transition bench clinic, highlighting key aspects, such structure biogenesis, methods, surface engineering techniques, applications. It also discusses challenges prospects this field.

Language: Английский

Citations

78
Extracellular vesicles engineering by silicates-activated endothelial progenitor cells for myocardial infarction treatment in male mice DOI Creative Commons
Bin Yu,

Hekai Li,

Zhaowenbin Zhang

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: April 13, 2023

Abstract Extracellular vesicles have shown good potential in disease treatments including ischemic injury such as myocardial infarction. However, the efficient production of highly active extracellular is one critical limitations for their clinical applications. Here, we demonstrate a biomaterial-based approach to prepare high amounts with bioactivity from endothelial progenitor cells (EPCs) by stimulation silicate ions derived bioactive ceramics. We further show that hydrogel microspheres containing engineered are effective treatment infarction male mice significantly enhancing angiogenesis. This therapeutic effect attributed enhanced revascularization content miR-126a-3p and angiogenic factors VEGF SDF-1, CXCR4 eNOS vesicles, which not only activate but also recruit EPCs circulatory system.

Language: Английский

Citations

48