Cancer Informatics,
Journal Year:
2022,
Volume and Issue:
21
Published: Jan. 1, 2022
Nuclear
paraspeckles
are
subnuclear
bodies
contracted
by
nuclear-enriched
abundant
transcript
1
(NEAT1)
long
non-coding
RNA,
localised
in
the
interchromatin
space
of
mammalian
cell
nuclei.
Paraspeckles
have
been
critically
involved
tumour
progression,
metastasis
and
chemoresistance.
To
this
date,
there
limited
findings
to
suggest
that
paraspeckles,
NEAT1
heterogeneous
nuclear
ribonucleoproteins
(hnRNPs)
directly
or
indirectly
play
roles
osteosarcoma
progression.
Herein,
we
analysed
NEAT1,
paraspeckle
proteins
(SFPQ,
PSPC1
NONO)
hnRNP
members
(HNRNPK,
HNRNPM,
HNRNPR
HNRNPD)
gene
expression
6
tissues
using
single-cell
RNA-sequencing
method.
The
normalised
data
highlighted
transcripts
were
highly
osteoblastic
OS
cells,
except
which
was
expressed
myeloid
2
subpopulations.
Ageing Research Reviews,
Journal Year:
2023,
Volume and Issue:
86, P. 101878 - 101878
Published: Feb. 3, 2023
Neurodegenerative
diseases
are
the
most
common
causes
of
disability
worldwide.
Given
their
high
prevalence,
devastating
symptoms,
and
lack
definitive
diagnostic
tests,
there
is
an
urgent
need
to
identify
potential
biomarkers
new
therapeutic
targets.
Long
non-coding
RNAs
(lncRNAs)
have
recently
emerged
as
powerful
regulatory
molecules
in
neurodegenerative
diseases.
Among
them,
lncRNA
nuclear
paraspeckle
assembly
transcript
1
(NEAT1)
has
been
reported
be
upregulated
Alzheimer's
disease
(AD),
Parkinson's
(PD),
Huntington's
(HD),
amyotrophic
lateral
sclerosis
(ALS).
However,
whether
this
part
a
protective
or
harmful
mechanism
still
unclear.
This
review
summarizes
our
current
knowledge
role
NEAT1
its
association
with
characteristic
aggregation
misfolded
proteins:
amyloid-β
tau
AD,
α-synuclein
PD,
mutant
huntingtin
HD,
TAR
DNA-binding
protein-43
fused
sarcoma/translocated
liposarcoma
ALS.
The
aim
stimulate
further
research
on
more
precise
effective
treatments
for
PubMed,
Journal Year:
2024,
Volume and Issue:
23, P. 34 - 52
Published: Jan. 1, 2024
This
review
delves
into
the
pivotal
role
of
long
non-coding
RNA
NEAT1
in
cancer
biology,
particularly
lung
(LC).
It
emphasizes
NEAT1's
unique
subcellular
localization
and
active
involvement
gene
regulation
chromatin
remodeling.
The
highlights
impact
on
LC
development
progression,
including
cell
processes
such
as
proliferation,
migration,
invasion,
resistance
to
therapy,
positioning
it
a
potential
diagnostic
marker
therapeutic
target.
complex
web
regulatory
interactions
with
proteins
microRNAs
is
explored,
alongside
challenges
targeting
therapeutically.
concludes
optimistically,
suggesting
future
avenues
for
research
personalized
therapies,
shedding
light
crucial
LC.
See
also
Graphical
abstract(Fig.
1).
Cancer Communications,
Journal Year:
2024,
Volume and Issue:
44(4), P. 469 - 490
Published: March 21, 2024
Abstract
Background
Chemoresistance
is
a
major
cause
of
treatment
failure
in
gastric
cancer
(GC).
Heterogeneous
nuclear
ribonucleoprotein
A2B1
(hnRNPA2B1)
an
N6‐methyladenosine
(m
6
A)‐binding
protein
involved
variety
cancers.
However,
whether
m
A
modification
and
hnRNPA2B1
play
role
GC
chemoresistance
largely
unknown.
In
this
study,
we
aimed
to
investigate
the
downstream
mechanism
chemoresistance.
Methods
The
expression
among
public
datasets
were
analyzed
validated
by
quantitative
PCR
(qPCR),
Western
blotting,
immunofluorescence,
immunohistochemical
staining.
biological
functions
investigated
both
vitro
vivo.
RNA
sequencing,
methylated
immunoprecipitation,
stability
assay
performed
assess
association
between
binding
RNA.
maintenance
stemness
was
evaluated
bioinformatic
analysis,
qPCR,
sphere
formation
assays.
patterns
regulators
specimens
from
patients
who
received
adjuvant
chemotherapy
RNAscope
multiplex
immunohistochemistry.
Results
Elevated
found
cells
tissues,
especially
multidrug‐resistant
(MDR)
cell
lines.
associated
with
poor
outcomes
patients,
those
5‐fluorouracil
treatment.
Silencing
effectively
sensitized
inhibiting
proliferation
inducing
apoptosis
Mechanically,
interacted
stabilized
long
noncoding
NEAT1
A‐dependent
manner.
Furthermore,
worked
together
enhance
properties
via
Wnt/β‐catenin
signaling
pathway.
clinical
subjected
chemotherapy,
levels
hnRNPA2B1,
NEAT1,
CD133,
CD44
markedly
elevated
non‐responders
compared
responders.
Conclusion
Our
findings
indicated
that
interacts
stabilizes
lncRNA
which
contribute
property
pathway
exacerbate
GC.
Journal of Translational Medicine,
Journal Year:
2022,
Volume and Issue:
20(1)
Published: Feb. 5, 2022
Abstract
Background
Long
noncoding
RNA
NEAT1
has
been
implicated
in
glioma
progression.
However,
the
effect
of
on
glycolysis
cell
and
potential
mechanism
remain
unclear.
Methods
In
vitro
experiments,
including
CCK-8,
colony
formation,
ECAR,
lactate
detection
assays
were
performed
to
evaluate
proliferation
cell.
pulldown
RIP
identify
interaction
between
PGK1.
Truncated
mutation
PGK1
was
used
confirm
specific
interactive
domains
Animal
studies
analyze
NEAT1/PGK1
Results
knockdown
significantly
suppressed
cells.
could
specifically
interact
with
PGK1,
which
promotes
stability.
Hairpin
A
is
essential
for
M1
domain
Depletion
markedly
inhibited
tumor
growth
mice,
while
reverse
this
effect.
Higher
expression
associated
poor
overall
survival
GBM
patients.
Conclusions
over
progression
through
stabilizing
axis
a
candidate
therapeutic
target
treatment.
Journal of Inflammation Research,
Journal Year:
2022,
Volume and Issue:
Volume 15, P. 557 - 571
Published: Jan. 1, 2022
Abstract:
Nuclear
paraspeckle
assembly
transcript
1
(Neat1)
located
at
chromosome
11
is
a
long
non-coding
RNA
that
widely
expressed
in
mammalian
cell
types,
and
which
overexpressed
several
inflammation-related
disorders.
Inflammation
implies
plethora
of
mutual
interactions
between
both
soluble
factors
cells
due
to
various
stimuli
including
tissue
injury.
Although
there
no
doubt
inflammation
critically
involved
multiple
biological
pathological
processes
alike,
the
precise
mechanisms
being
are
still
open
for
debate.
In
this
context,
role
Neat1
as
regulator
inflammation,
microglial
activation,
lipid
accumulation
under
inflammatory
conditions
remains
elusive.
Herein,
we
review
regulation
how
it
modulates
expression
its
target
genes.
Thereafter,
will
impact
on
by
activating
or
inhibiting
signaling
pathways,
such
microRNAs,
AKT,
TLR4,
TRAF6,
NF-κB.
Keywords:
Neat1,
microglia,
lipids,
microRNA,
NF-κB
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(8), P. 4548 - 4548
Published: April 21, 2024
Colorectal
cancer
(CRC)
is
the
third
most
prevalent
to
be
diagnosed,
and
it
has
a
substantial
mortality
rate.
Despite
numerous
studies
being
conducted
on
CRC,
remains
significant
health
concern.
The
disease-free
survival
rates
notably
decrease
as
CRC
progresses,
emphasizing
urgency
for
effective
diagnostic
therapeutic
approaches.
development
caused
by
environmental
factors,
which
mostly
lead
disruption
of
signaling
pathways.
Among
these
pathways,
Wingless/Integrated
(Wnt)
pathway,
Phosphatidylinositol
3-kinase/protein
kinase
B/mammalian
target
rapamycin
(PI3K/AKT/mTOR)
Mitogen-Activated
Protein
Kinase
(MAPK)
Transforming
Growth
Factor-β
(TGF-β)
p53
pathway
are
considered
important.
These
pathways
also
regulated
non-coding
RNAs
(ncRNAs),
including
microRNAs
(miRNAs),
long
(lncRNAs),
circular
(circRNAs).
They
have
emerged
crucial
regulators
gene
expression
in
changing
their
levels.
altered
patterns
ncRNAs
been
implicated
progression
development,
suggesting
potential
targets.
This
review
provides
an
overview
five
key
regulation
involved
pathogenesis
that
studied
identify
promising
avenues
diagnosis
treatment
strategies.
Non-coding RNA Research,
Journal Year:
2024,
Volume and Issue:
9(2), P. 547 - 559
Published: Jan. 11, 2024
A
non-negligible
part
of
our
DNA
has
been
proven
to
be
transcribed
into
non-protein
coding
RNA
and
its
intricate
involvement
in
several
physiological
processes
highly
evidenced.
The
significant
biological
role
non-coding
RNAs
(ncRNAs),
including
long
(lncRNAs)
variously
reported.
In
the
current
review,
authors
highlight
multifaceted
myocardial
infarction-associated
transcript
(
