The astrocyte-enriched gene deathstar plays a crucial role in the development, locomotion, and lifespan of D. melanogaster DOI Creative Commons
Xiaoli Zhang,

Dongyu Sun,

Kyle Wong

et al.

Fly, Journal Year: 2024, Volume and Issue: 18(1)

Published: June 17, 2024

The Drosophila melanogaster brain is a complex organ with various cell types, orchestrating the development, physiology, and behaviors of fly. While each type in known to express unique gene set, their complete genetic profile still unknown. Advances RNA sequencing techniques at single-cell resolution facilitate identifying novel markers and/or re-examining specificity available ones. In this study, exploiting data optic lobe, we categorized cells based on expression pattern for markers, then genes enriched astrocytes were identified. CG11000 was identified as comparable Eaat1 gene, an astrocyte marker, every individual inside lobe midbrain, well entire throughout its development. Consistent our bioinformatics data, immunostaining brains dissected from transgenic adult flies showed co-expression set single corresponding brain. Physiologically, inhibiting through interference disrupted normal development male D. melanogaster, while having no impact females. Expression suppression led decreased locomotion activity also shortened lifespan specifically astrocytes, indicating gene's significance astrocytes. We designated 'deathstar' due crucial role maintaining star-like shape glial cells, into stage.

Language: Английский

Defective cortex glia plasma membrane structure underlies light-induced epilepsy in cpes mutants DOI Creative Commons
Kunduri Govind, Daniel B. Turner‐Evans, Yutaka Konya

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2018, Volume and Issue: 115(38)

Published: Sept. 5, 2018

Seizures induced by visual stimulation (photosensitive epilepsy; PSE) represent a common type of epilepsy in humans, but the molecular mechanisms and genetic drivers underlying PSE remain unknown, no good animal models have been identified as yet. Here, we show an model PSE, Drosophila, owing to defective cortex glia. The glial membranes are severely compromised ceramide phosphoethanolamine synthase (cpes)-null mutants fail encapsulate neuronal cell bodies Drosophila cortex. Expression human sphingomyelin 1, which synthesizes closely related phosphocholine (sphingomyelin), rescues abnormalities underscoring evolutionarily conserved role these lipids membranes. Further, compromise plasma membrane structure that underlies collapse cpes leads phenotype.

Language: Английский

Citations

37
Pvr receptor tyrosine kinase signaling promotes post-embryonic morphogenesis, and survival of glia and neural progenitor cells in Drosophila DOI Open Access
Renee Read

Development, Journal Year: 2018, Volume and Issue: 145(23)

Published: Oct. 16, 2018

Stem cells reside in specialized microenvironments, called niches, that regulate their development and the of progeny. However, maintenance niches are poorly understood. In Drosophila brain, cortex glial provide a niche promotes self-renewal proliferation neural stem cell-like (neuroblasts). central neuroblasts progeny control post-embryonic morphogenesis glia through PDGF-like ligands, this PDGFR receptor tyrosine kinase (RTK) signaling is required for expression DE-cadherin, which sustains neuroblasts. Thus, an RTK-dependent feed-forward loop, actively maintain each other. When EGFR RTK constitutively activated glia, they overexpress PDGF orthologs to stimulate autocrine signaling, uncouples growth survival from neuroblasts, drives neoplastic transformation elimination These results fundamental insights into regulation, show niche-neural cell becomes hijacked drive tumorigenesis.

Language: Английский

Citations

36
Glial Hedgehog signalling and lipid metabolism regulate neural stem cell proliferation in Drosophila DOI Creative Commons
Qian Dong, Michael Zavortink,

Francesca Froldi

et al.

EMBO Reports, Journal Year: 2021, Volume and Issue: 22(5)

Published: March 10, 2021

Language: Английский

Citations

26
Redundant functions of the SLC5A transporters Rumpel, Bumpel, and Kumpel in ensheathing glial cells DOI Creative Commons
Kerem Yildirim, Bente Winkler,

Nicole Pogodalla

et al.

Biology Open, Journal Year: 2021, Volume and Issue: 11(1)

Published: Dec. 13, 2021

Neuronal processing is energy demanding and relies on sugar metabolism. To nurture the Drosophila nervous system, blood-brain barrier forming glial cells take up trehalose from hemolymph then distribute metabolic products further to all neurons. This function provided by glucose lactate transporters of solute carrier (SLC) 5A family. Here we identified three SLC5A genes that are specifically expressed in overlapping sets CNS cells, rumpel, bumpel kumpel. We generated mutants viable fertile, lacking discernible phenotypes. Loss rumpel causes subtle locomotor phenotypes flies display increased daytime sleep. In addition, kumpel double mutants, an even greater extent triple oogenesis disrupted at onset vitollegenic phase. indicates a partially redundant between these genes. Rescue experiments exploring this effect indicate can be affected cells. Moreover, expression heterologous mammalian transporters, with known transport properties, suggest Bumpel and/or Kumpel or lactate. Overall, our results imply redundancy nutrient sensing functions affecting ovarian development behavior.

Language: Английский

Citations

24
An interplay between cellular growth and atypical fusion defines morphogenesis of a modular glial niche in Drosophila DOI Creative Commons
Maria A. Rujano, David Briand,

Bojana Ðelić

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Aug. 25, 2022

Abstract Neural stem cells (NSCs) live in an intricate cellular microenvironment supporting their activity, the niche. Whilst shape and function are inseparable, morphogenetic aspects of niche development poorly understood. Here, we use formation a glial to investigate acquisition architectural complexity. Cortex glia (CG) Drosophila regulate neurogenesis build reticular structure around NSCs. We first show that individual CG grow tremendously ensheath several NSC lineages, employing elaborate proliferative mechanisms which convert these into syncytia rich cytoplasmic bridges. further undergo homotypic cell–cell fusion, using defined cell surface receptors actin regulators. Cellular exchange is however dynamic space time. This atypical fusion remodels borders, restructuring syncytia. Ultimately, combined growth builds multi-level architecture niche, creates modular, spatial partition population. Our findings provide insights how forms organises while developing intimate contacts with

Language: Английский

Citations

18
GABAB receptors regulate the neural stem cell potential of Pkd2l1+ cerebrospinal fluid-contacting neurons via the PI3K/Akt signaling pathway DOI Creative Commons
Liang Cao,

Weihong Yan,

Wenjun Pi

et al.

Brain Research Bulletin, Journal Year: 2025, Volume and Issue: 221, P. 111217 - 111217

Published: Jan. 20, 2025

Language: Английский

Citations

0
Animal-based approaches to understanding neuroglia physiology in vitro and in vivo DOI
Davide Gobbo, Frank Kirchhoff

Handbook of clinical neurology, Journal Year: 2025, Volume and Issue: unknown, P. 229 - 263

Published: Jan. 1, 2025

Language: Английский

Citations

0
The Critical Balance Between Quiescence and Reactivation of Neural Stem Cells DOI Creative Commons
Adam M. Elkin, Sarah Robbins, Claudia S. Barros

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(5), P. 672 - 672

Published: May 6, 2025

Neural stem cells (NSC) are multipotent, self-renewing that give rise to all neural cell types within the central nervous system. During adulthood, most NSCs exist in a quiescent state which can be reactivated response metabolic and signalling changes, allowing for long-term continuous neurogenesis injury. Ensuring critical balance between quiescence reactivation is required maintain limited NSC reservoir replenishment throughout lifetime. The precise mechanisms pathways behind this at focus of current research. In review, we highlight discuss recent studies using Drosophila, mammalian zebrafish models contributing understanding molecular underlying NSCs.

Language: Английский

Citations

0
Hsp83/Hsp90 Physically Associates with Insulin Receptor to Promote Neural Stem Cell Reactivation DOI Creative Commons
Jiawen Huang, Hongyan Wang

Stem Cell Reports, Journal Year: 2018, Volume and Issue: 11(4), P. 883 - 896

Published: Sept. 20, 2018

Neural stem cells (NSCs) have the ability to exit quiescence and reactivate in response physiological stimuli. In Drosophila brain, insulin receptor (InR)/phosphatidylinositol 3-kinase (PI3K)/Akt pathway triggers NSC reactivation. However, intrinsic mechanisms that control InR/PI3K/Akt during reactivation remain unknown. Here, we identified heat shock protein 83 (Hsp83/Hsp90), a molecular chaperone, as an regulator of Hsp83 is both necessary sufficient for by promoting activation InR larval brains presence dietary amino acids. Both its co-chaperone Cdc37 physically associate with InR. Finally, defects observed depleted hsp83 were rescued over-activation pathway, suggesting functions upstream Given conservation our finding may provide insights into underlying mammalian

Language: Английский

Citations

28
Sexual dimorphism of niche architecture and regulation of theCaenorhabditis elegansgermline stem cell pool DOI Creative Commons

Sarah L. Crittenden,

ChangHwan Lee, Ipsita Mohanty

et al.

Molecular Biology of the Cell, Journal Year: 2019, Volume and Issue: 30(14), P. 1757 - 1769

Published: May 8, 2019

Stem cell maintenance by niche signaling is a common theme across phylogeny. In the Caenorhabditis elegans gonad, broad outlines of germline stem (GSC) regulation are same for both sexes: GLP-1/Notch from mesenchymal distal tip maintains GSCs in gonad sexes and does so via two key regulators, SYGL-1 LST-1. Yet most recent analyses GSC have focused on XX hermaphrodites, an essentially female sex making sperm larvae oocytes adults. Here we focus XO males. Sexual dimorphism architecture, reported previously, suggested that molecular responses to or numbers might also be sexually distinct. Remarkably, this not case. This work extends our understanding dimorphic but demonstrates niches drive similar response maintain number their pools.

Language: Английский

Citations

27