Journal of Biological Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown, P. 108329 - 108329
Published: Feb. 1, 2025
Adaptations
to
fluctuating
environmental
conditions
require
bacteria
make
large
scale
proteomic
shifts
on
short
timescales.
We
previously
characterised
the
tri-partite
RimABK
protein
complex
responsible
for
post
translational
modification
of
ribosome
in
response
cues.
Regulated
control
RpsF
polyglutamylation
by
RimK
rapidly
influenced
proteome
Pseudomonas
fluorescens
cells
facilitate
colonisation
plant
rhizosphere.
Here,
we
conduct
a
detailed
investigation
RimB
protease.
show
be
bifunctional
retropepsin-like
aspartic
endopeptidase
that
uniquely
recognises
and
removes
glutamate
residues
from
polyglutamated
stimulates
poly-α-L-glutamate
synthesis
RimK.
determine
minimal
recognition
requirements
proteolysis
identify
catalytic
aspartate
residue
required
function.
Further,
novel
hybrid
enzyme
composed
domains
also
possesses
protease
activity.
Phylogenetic
analysis
accessions
encoding
either
or
individual
proteins
reveals
pattern
rim
gene
evolution
is
distinct
host
organisms
potential
alternative
targets
RimB.
Virulence,
Journal Year:
2021,
Volume and Issue:
12(1), P. 1122 - 1144
Published: April 12, 2021
Bacteria
of
the
genus
Legionella
are
natural
pathogens
amoebae
that
can
cause
a
severe
pneumonia
in
humans
called
Legionnaires'
Disease.
Human
disease
results
from
inhalation
Legionella-contaminated
aerosols
and
subsequent
bacterial
replication
within
alveolar
macrophages.
pathogenicity
has
resulted
extensive
co-evolution
with
diverse
genera
amoebae.
To
replicate
intracellularly,
generates
replication-permissive
compartment
Legionella-containing
vacuole
(LCV)
through
concerted
action
hundreds
Dot/Icm-translocated
effector
proteins.
In
this
review,
we
present
collective
overview
including
infection
mechanisms,
secretion
systems,
translocated
function.
We
also
discuss
innate
adaptive
immune
responses
to
L.
pneumophila,
implications
genome
diversity
future
avenues
for
field.
Proceedings of the National Academy of Sciences,
Journal Year:
2019,
Volume and Issue:
116(47), P. 23518 - 23526
Published: Nov. 5, 2019
Posttranslational
protein
modification
by
ubiquitin
(Ub)
is
a
central
eukaryotic
mechanism
that
regulates
plethora
of
physiological
processes.
Recent
studies
unveiled
an
unconventional
type
ubiquitination
mediated
the
SidE
family
Legionella
pneumophila
effectors,
such
as
SdeA,
catalyzes
conjugation
Ub
to
serine
residue
target
proteins
via
phosphoribosyl
linker
(hence
named
PR-ubiquitination).
Comparable
deubiquitinases
in
canonical
pathway,
here
we
show
2
paralogous
Lpg2154
(DupA;
deubiquitinase
for
PR-ubiquitination)
and
Lpg2509
(DupB),
reverse
PR-ubiquitination
specific
removal
phosphoribosyl-Ub
from
substrates.
Both
DupA
DupB
are
fully
capable
rescuing
Golgi
fragmentation
phenotype
caused
exogenous
expression
SdeA
mammalian
cells.
We
further
deletion
these
genes
results
significant
accumulation
PR-ubiquitinated
species
host
cells
infected
with
In
addition,
have
identified
list
targets
play
role
modulating
association
Legionella-containing
vacuoles.
Together,
our
data
establish
complete
deubiquitination
cycle
demonstrate
intricate
control
has
over
this
unusual
Ub-dependent
posttranslational
modification.
Cell,
Journal Year:
2022,
Volume and Issue:
185(13), P. 2354 - 2369.e17
Published: May 13, 2022
Interferons
(IFNs)
induce
an
antimicrobial
state,
protecting
tissues
from
infection.
Many
viruses
inhibit
IFN
signaling,
but
whether
bacterial
pathogens
evade
responses
remains
unclear.
Here,
we
demonstrate
that
the
Shigella
OspC
family
of
type-III-secreted
effectors
blocks
signaling
independently
its
cell
death
inhibitory
activity.
Rather,
inhibition
was
mediated
by
binding
OspC1
and
OspC3
to
Ca2+
sensor
calmodulin
(CaM),
blocking
CaM
kinase
II
downstream
JAK/STAT
signaling.
The
growth
lacking
attenuated
in
epithelial
cells
a
murine
model
This
phenotype
rescued
both
models
depletion
receptors.
homologs
conserved
additional
not
only
bound
also
inhibited
IFN,
suggesting
widespread
virulence
strategy.
These
findings
reveal
previously
undescribed
molecular
mechanism
critical
role
targeting
pathogenesis.
Through
coevolution
with
host
cells,
microorganisms
have
acquired
mechanisms
to
avoid
the
detection
by
surveillance
system
and
use
cell’s
supplies
establish
themselves.
Indeed,
certain
pathogens
evolved
proteins
that
imitate
specific
eukaryotic
cell
proteins,
allowing
them
manipulate
pathways,
a
phenomenon
termed
molecular
mimicry.
Bacterial
“eukaryotic-like
proteins”
are
remarkable
example
of
They
defined
as
strongly
resemble
or
carry
domains
predominantly
present
in
eukaryotes
generally
absent
from
prokaryotes.
Legionella
pneumophila
extensively
modulates
the
host
ubiquitin
network
to
create
Legionella-containing
vacuole
(LCV)
for
its
replication.
Many
of
virulence
factors
function
as
ligases
or
deubiquitinases
(DUBs).
Here,
we
identify
Lem27
a
DUB
that
displays
preference
diubiquitin
formed
by
K6,
K11,
K48.
is
associated
with
LCV
where
it
regulates
Rab10
ubiquitination
in
concert
SidC
and
SdcA,
two
bacterial
E3
ligases.
Structural
analysis
complex
an
active
fragment
substrate-based
suicide
inhibitor
ubiquitin-propargylamide
(PA)
reveals
harbors
fold
resembling
those
OTU1
subfamily
Cys-His
catalytic
dyad
recognizes
via
extensive
hydrogen
bonding
at
six
contact
sites.
Our
results
establish
functions
regulate
protein
on
L.
phagosomes
counteracting
activity
Computational and Structural Biotechnology Journal,
Journal Year:
2021,
Volume and Issue:
19, P. 2366 - 2383
Published: Jan. 1, 2021
ADP-ribosylation
is
an
ancient
posttranslational
modification
present
in
all
kingdoms
of
life.
The
system
likely
originated
bacteria
where
it
functions
inter-
and
intra-species
conflict,
stress
response
pathogenicity.
It
was
repeatedly
adopted
via
lateral
transfer
by
eukaryotes,
including
humans,
has
a
pivotal
role
epigenetics,
DNA-damage
repair,
apoptosis,
other
crucial
pathways
the
immune
to
pathogenic
viruses.
In
words,
same
ammunition
used
pathogens
adapted
eukaryotes
fight
back.
While
we
know
quite
lot
about
eukaryotic
system,
expanding
rather
patchy
knowledge
on
bacterial
viral
would
give
us
not
only
better
understanding
as
whole
but
fighting
advantage
this
constant
arms
race.
By
writing
review
hope
put
into
focus
available
information
perspective
how
works
can
be
exploited
search
for
therapeutic
targets
future.
relevance
subject
especially
highlighted
current
situation
being
amid
world
pandemic
caused
virus
harbouring
dependent
representative
such
system.
Microbiology,
Journal Year:
2022,
Volume and Issue:
168(5)
Published: May 23, 2022
To
prevail
in
the
interaction
with
eukaryotic
hosts,
many
bacterial
pathogens
use
protein
secretion
systems
to
release
virulence
factors
at
host–pathogen
interface
and/or
deliver
them
directly
into
host
cells.
An
outstanding
example
of
complexity
and
sophistication
diversity
their
substrates,
effectors,
is
Defective
organelle
trafficking/Intracellular
multiplication
(Dot/Icm)
Type
IVB
system
(T4BSS)
Legionella
pneumophila
related
species.
species
are
facultative
intracellular
environmental
protozoa
opportunistic
human
respiratory
pathogens.
The
Dot/Icm
T4BSS
translocates
an
exceptionally
large
number
more
than
300
per
L.
strain,
essential
for
evasion
phagolysosomal
degradation
exploitation
macrophages
as
replicative
niches.
Recent
technological
advancements
imaging
complexes
have
provided
new
insight
architecture
allowed
us
propose
models
transport
mechanism.
At
same
time,
significant
progress
has
been
made
assigning
functions
about
a
third
discovering
unprecedented
enzymatic
activities
concepts
subversion.
In
this
review,
we
describe
current
knowledge
workings
machinery
provide
overview
to-date
characterized
effectors
Legionella
pneumophila
promotes
its
survival
and
replication
in
phagocytes
by
actively
modulating
cellular
processes
using
effectors
injected
into
host
cells
Dot/Icm
type
IV
secretion
system.
Many
of
these
function
to
manipulate
the
ubiquitin
network
infected
cells,
thus
contributing
biogenesis
Legionella-containing
vacuole
(LCV),
which
is
permissive
for
bacterial
replication.
Among
these,
members
SidE
effector
family
(SidEs)
catalyze
ubiquitination
functionally
diverse
proteins
a
mechanism
that
chemically
distinct
from
canonical
three-enzyme
cascade.
The
activity
SidEs
regulated
two
mechanisms:
reversal
phosphoribosyl
DupA
DupB
direct
inactivation
SidJ,
calmodulin-dependent
glutamylase.
In
many
L.
strains,
SidJ
belongs
two-member
protein
family.
Its
homolog
SdjA
appears
differently
despite
high-level
similarity
their
primary
sequences.
Here,
we
found
bifunctional
enzyme
exhibits
activities
toward
It
inhibits
SdeB
SdeC
glutamylation.
Unexpectedly,
it
also
functions
as
deglutamylase
reverses
SidJ-induced
glutamylation
on
SdeA.
Our
results
reveal
an
can
completely
opposite
biochemical
reactions,
highlights
regulation
IMPORTANCE
One
unique
feature
existence
families
with
similarity.
Whereas
some
are
redundant,
suggested
sequences,
relationship
between
SdjA,
family,
has
remained
mysterious.
Despite
sharing
57%
identity,
sdjA
cannot
complement
defects
virulence
displayed
mutant
lacking
sidJ.
calmodulin
(CaM)-dependent
glutamylase
activity.
dual
protein:
CaM-dependent
against
but
SdeA
been
modified
indicating
fine-tune
SidEs.
These
findings
have
paved
way
future
structural
functional
analysis
may
novel
isopeptide
bond
cleavage
provide
insights
study
evolution.